Calprotectin’s Protein Structure Shields Ni–N(His) Bonds from Competing Agents

The Ni–N­(His) coordination bond, formed between the nickel ion and histidine residues, is essential for recombinant protein purification, especially in Ni-NTA-based systems for selectively binding polyhistidine-tagged (Histag) proteins. While previous studies have explored its bond strength in a sy...

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Veröffentlicht in:	The journal of physical chemistry letters 2025-02, Vol.16 (5), p.1282-1287
Hauptverfasser:	Lu, Zhuojian, Nie, Jingyuan, Wang, Ziling, Wang, Ziyi, Zhang, Panke, Jiang, Yajun, Zheng, Peng
Format:	Artikel
Sprache:	eng
Schlagworte:	Histidine - chemistry Imidazoles - chemistry Kinetics Leukocyte L1 Antigen Complex - chemistry Microscopy, Atomic Force Nickel - chemistry Physical Insights into Materials and Molecular Properties Protein Conformation
Online-Zugang:	Volltext
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