Exogenous dsRNA-Mediated RNAi: Mechanisms, Applications, Delivery Methods and Challenges in the Induction of Viral Disease Resistance in Plants
The increasing challenges posed by plant viral diseases demand innovative and sustainable management strategies to minimize agricultural losses. Exogenous double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) represents a transformative approach to combat plant viral pathogens without the nee...
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| Veröffentlicht in: | Viruses 2024-12, Vol.17 (1), p.49 |
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| creator | Venu, Emmadi Ramya, Akurathi Babu, Pedapudi Lokesh Srinivas, Bhukya Kumar, Sathiyaseelan Reddy, Namburi Karunakar Babu, Yeluru Mohan Majumdar, Anik Manik, Suryakant |
| description | The increasing challenges posed by plant viral diseases demand innovative and sustainable management strategies to minimize agricultural losses. Exogenous double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) represents a transformative approach to combat plant viral pathogens without the need for genetic transformation. This review explores the mechanisms underlying dsRNA-induced RNAi, highlighting its ability to silence specific viral genes through small interfering RNAs (siRNAs). Key advancements in dsRNA production, including cost-effective microbial synthesis and in vitro methods, are examined alongside delivery techniques such as spray-induced gene silencing (SIGS) and nanocarrier-based systems. Strategies for enhancing dsRNA stability, including the use of nanomaterials like layered double hydroxide nanosheets and carbon dots, are discussed to address environmental degradation challenges. Practical applications of this technology against various plant viruses and its potential to ensure food security are emphasized. The review also delves into regulatory considerations, risk assessments, and the challenges associated with off-target effects and pathogen resistance. By evaluating both opportunities and limitations, this review underscores the role of exogenous dsRNA as a sustainable solution for achieving viral disease resistance in plants. |
| doi_str_mv | 10.3390/v17010049 |
| format | Article |
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Exogenous double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) represents a transformative approach to combat plant viral pathogens without the need for genetic transformation. This review explores the mechanisms underlying dsRNA-induced RNAi, highlighting its ability to silence specific viral genes through small interfering RNAs (siRNAs). Key advancements in dsRNA production, including cost-effective microbial synthesis and in vitro methods, are examined alongside delivery techniques such as spray-induced gene silencing (SIGS) and nanocarrier-based systems. Strategies for enhancing dsRNA stability, including the use of nanomaterials like layered double hydroxide nanosheets and carbon dots, are discussed to address environmental degradation challenges. Practical applications of this technology against various plant viruses and its potential to ensure food security are emphasized. 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| subjects | Acids Agriculture Cotton Crop diseases Disease resistance Disease Resistance - genetics Double-stranded RNA dsRNA stability E coli Environmental degradation exogenous dsRNA Food plants Food security Gene expression Gene silencing Genetic transformation Methods MicroRNAs nanocarriers Nanotechnology Pathogens Phages Plant Diseases - genetics Plant Diseases - immunology Plant Diseases - prevention & control Plant Diseases - virology Plant resistance plant viral diseases Plant viruses Plant Viruses - genetics Plants - genetics Plants - virology Review Reviews RNA Interference RNA interference (RNAi) RNA polymerase RNA, Double-Stranded - genetics RNA, Small Interfering - genetics RNA-mediated interference siRNA spray-induced gene silencing (SIGS) Viruses |
| title | Exogenous dsRNA-Mediated RNAi: Mechanisms, Applications, Delivery Methods and Challenges in the Induction of Viral Disease Resistance in Plants |
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