Expression of fatty acid binding proteins in mesenteric adipose tissue

Adipose is a complex tissue comprised of adipocytes, immune cells, endothelial and progenitor stem cells. In humans, there are at least nine defined adipose depots, each containing variable numbers of genetically identified adipocyte clusters suggesting remarkable heterogeneity and potential functio...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biochemical and biophysical research communications 2025-02, Vol.749, p.151346, Article 151346
Hauptverfasser: Fish, Shayla R., Halley, Catherine L., Dileepan, Mythili, Hertzel, Ann V., Dickey, Deborah M., Bernlohr, David A.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page 151346
container_title Biochemical and biophysical research communications
container_volume 749
creator Fish, Shayla R.
Halley, Catherine L.
Dileepan, Mythili
Hertzel, Ann V.
Dickey, Deborah M.
Bernlohr, David A.
description Adipose is a complex tissue comprised of adipocytes, immune cells, endothelial and progenitor stem cells. In humans, there are at least nine defined adipose depots, each containing variable numbers of genetically identified adipocyte clusters suggesting remarkable heterogeneity and potential functionality in each depot with respect to lipid metabolism. Although subcutaneous and visceral depots are commonly analyzed for biochemical and molecular functions, the mesenteric depot has been overlooked yet strongly implicated in lipid mediated immune surveillance. Since fatty acid binding proteins (FABPs) are primary cellular conduits to lipid trafficking, we evaluated the expression patterns for four major fatty acid binding proteins (FABP1, FABP3, FABP4 and FABP5) using a combination of gene expression, immunoblotting, and immunofluorescence in mesenteric fat from both young and old, male and female C57Bl/6J mice. All four FABPs were expressed at the mRNA and protein level in murine mesenteric adipose tissue. While there was no statistical change in expression of mesenteric FABP isoforms with sex or age, the expression of mesenteric FABP1 was increased, and FABP4 decreased, in both males and females as compared to perigonadal and inguinal depots. Surprisingly, immunofluorescence staining revealed that compared to subcutaneous or perigonadal depots, mesenteric fat expresses FABP3, but little FABP5, in adipocytes. These results highlight the diversity in adipose tissue and the importance of evaluating the mesenteric depot in the context of lipid transport and metabolism. •FABP3 is expressed to high levels in mesenteric adipocytes.•FABP5 is expressed in the mesenteric stromal vascular fraction but undetectable in mesenteric adipocytes.•FABP1 expression is highest in mesenteric fat compared to FABP1 expression in inguinal or perigonadal fat.•FABP4 expression in highest expressing FABP in mesenteric adipose tissue.
doi_str_mv 10.1016/j.bbrc.2025.151346
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3159798291</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X25000609</els_id><sourcerecordid>3159798291</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1520-820dc4ea3df745155fa95824ab53516be8515b864df42f5dc8a8106187ac33e03</originalsourceid><addsrcrecordid>eNp9kMFKAzEQhoMotlZfwIPk6GXrTDbZZsGLlFaFghcFbyGbzEpKu1uTrdi3d0urR08Dw_f_zHyMXSOMEbC4W46rKrqxAKHGqDCXxQkbIpSQCQR5yoYAUGSixPcBu0hpCYAoi_KcDfJSKwUShmw--95ESim0DW9rXtuu23HrgudVaHxoPvgmth2FJvHQ8DUlajqKwXHrw6ZNxLuQ0pYu2VltV4mujnPE3uaz1-lTtnh5fJ4-LDKHSkCmBXgnyea-nkiFStW2VFpIW6lcYVGR7peVLqSvpaiVd9pqhAL1xLo8J8hH7PbQ21_1uaXUmXVIjlYr21C7TSZHVU5K3f_co-KAutimFKk2mxjWNu4Mgtn7M0uz92f2_szBXx-6OfZvqzX5v8ivsB64PwDUf_kVKJrkAjWOfIjkOuPb8F__D22RgF0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3159798291</pqid></control><display><type>article</type><title>Expression of fatty acid binding proteins in mesenteric adipose tissue</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Fish, Shayla R. ; Halley, Catherine L. ; Dileepan, Mythili ; Hertzel, Ann V. ; Dickey, Deborah M. ; Bernlohr, David A.</creator><creatorcontrib>Fish, Shayla R. ; Halley, Catherine L. ; Dileepan, Mythili ; Hertzel, Ann V. ; Dickey, Deborah M. ; Bernlohr, David A.</creatorcontrib><description>Adipose is a complex tissue comprised of adipocytes, immune cells, endothelial and progenitor stem cells. In humans, there are at least nine defined adipose depots, each containing variable numbers of genetically identified adipocyte clusters suggesting remarkable heterogeneity and potential functionality in each depot with respect to lipid metabolism. Although subcutaneous and visceral depots are commonly analyzed for biochemical and molecular functions, the mesenteric depot has been overlooked yet strongly implicated in lipid mediated immune surveillance. Since fatty acid binding proteins (FABPs) are primary cellular conduits to lipid trafficking, we evaluated the expression patterns for four major fatty acid binding proteins (FABP1, FABP3, FABP4 and FABP5) using a combination of gene expression, immunoblotting, and immunofluorescence in mesenteric fat from both young and old, male and female C57Bl/6J mice. All four FABPs were expressed at the mRNA and protein level in murine mesenteric adipose tissue. While there was no statistical change in expression of mesenteric FABP isoforms with sex or age, the expression of mesenteric FABP1 was increased, and FABP4 decreased, in both males and females as compared to perigonadal and inguinal depots. Surprisingly, immunofluorescence staining revealed that compared to subcutaneous or perigonadal depots, mesenteric fat expresses FABP3, but little FABP5, in adipocytes. These results highlight the diversity in adipose tissue and the importance of evaluating the mesenteric depot in the context of lipid transport and metabolism. •FABP3 is expressed to high levels in mesenteric adipocytes.•FABP5 is expressed in the mesenteric stromal vascular fraction but undetectable in mesenteric adipocytes.•FABP1 expression is highest in mesenteric fat compared to FABP1 expression in inguinal or perigonadal fat.•FABP4 expression in highest expressing FABP in mesenteric adipose tissue.</description><identifier>ISSN: 0006-291X</identifier><identifier>ISSN: 1090-2104</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2025.151346</identifier><identifier>PMID: 39855040</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adipocytes - metabolism ; Adipose Tissue - metabolism ; Animals ; Fatty Acid Binding Protein 3 - genetics ; Fatty Acid Binding Protein 3 - metabolism ; Fatty acid binding proteins ; Fatty Acid-Binding Proteins - genetics ; Fatty Acid-Binding Proteins - metabolism ; Female ; Lipid trafficking ; Male ; Mesentery ; Mesentery - metabolism ; Mice ; Mice, Inbred C57BL ; Neoplasm Proteins ; Visceral adipose</subject><ispartof>Biochemical and biophysical research communications, 2025-02, Vol.749, p.151346, Article 151346</ispartof><rights>2025 Elsevier Inc.</rights><rights>Copyright © 2025 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1520-820dc4ea3df745155fa95824ab53516be8515b864df42f5dc8a8106187ac33e03</cites><orcidid>0009-0008-6032-6105 ; 0000-0002-9172-7939</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X25000609$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39855040$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fish, Shayla R.</creatorcontrib><creatorcontrib>Halley, Catherine L.</creatorcontrib><creatorcontrib>Dileepan, Mythili</creatorcontrib><creatorcontrib>Hertzel, Ann V.</creatorcontrib><creatorcontrib>Dickey, Deborah M.</creatorcontrib><creatorcontrib>Bernlohr, David A.</creatorcontrib><title>Expression of fatty acid binding proteins in mesenteric adipose tissue</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Adipose is a complex tissue comprised of adipocytes, immune cells, endothelial and progenitor stem cells. In humans, there are at least nine defined adipose depots, each containing variable numbers of genetically identified adipocyte clusters suggesting remarkable heterogeneity and potential functionality in each depot with respect to lipid metabolism. Although subcutaneous and visceral depots are commonly analyzed for biochemical and molecular functions, the mesenteric depot has been overlooked yet strongly implicated in lipid mediated immune surveillance. Since fatty acid binding proteins (FABPs) are primary cellular conduits to lipid trafficking, we evaluated the expression patterns for four major fatty acid binding proteins (FABP1, FABP3, FABP4 and FABP5) using a combination of gene expression, immunoblotting, and immunofluorescence in mesenteric fat from both young and old, male and female C57Bl/6J mice. All four FABPs were expressed at the mRNA and protein level in murine mesenteric adipose tissue. While there was no statistical change in expression of mesenteric FABP isoforms with sex or age, the expression of mesenteric FABP1 was increased, and FABP4 decreased, in both males and females as compared to perigonadal and inguinal depots. Surprisingly, immunofluorescence staining revealed that compared to subcutaneous or perigonadal depots, mesenteric fat expresses FABP3, but little FABP5, in adipocytes. These results highlight the diversity in adipose tissue and the importance of evaluating the mesenteric depot in the context of lipid transport and metabolism. •FABP3 is expressed to high levels in mesenteric adipocytes.•FABP5 is expressed in the mesenteric stromal vascular fraction but undetectable in mesenteric adipocytes.•FABP1 expression is highest in mesenteric fat compared to FABP1 expression in inguinal or perigonadal fat.•FABP4 expression in highest expressing FABP in mesenteric adipose tissue.</description><subject>Adipocytes - metabolism</subject><subject>Adipose Tissue - metabolism</subject><subject>Animals</subject><subject>Fatty Acid Binding Protein 3 - genetics</subject><subject>Fatty Acid Binding Protein 3 - metabolism</subject><subject>Fatty acid binding proteins</subject><subject>Fatty Acid-Binding Proteins - genetics</subject><subject>Fatty Acid-Binding Proteins - metabolism</subject><subject>Female</subject><subject>Lipid trafficking</subject><subject>Male</subject><subject>Mesentery</subject><subject>Mesentery - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Neoplasm Proteins</subject><subject>Visceral adipose</subject><issn>0006-291X</issn><issn>1090-2104</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFKAzEQhoMotlZfwIPk6GXrTDbZZsGLlFaFghcFbyGbzEpKu1uTrdi3d0urR08Dw_f_zHyMXSOMEbC4W46rKrqxAKHGqDCXxQkbIpSQCQR5yoYAUGSixPcBu0hpCYAoi_KcDfJSKwUShmw--95ESim0DW9rXtuu23HrgudVaHxoPvgmth2FJvHQ8DUlajqKwXHrw6ZNxLuQ0pYu2VltV4mujnPE3uaz1-lTtnh5fJ4-LDKHSkCmBXgnyea-nkiFStW2VFpIW6lcYVGR7peVLqSvpaiVd9pqhAL1xLo8J8hH7PbQ21_1uaXUmXVIjlYr21C7TSZHVU5K3f_co-KAutimFKk2mxjWNu4Mgtn7M0uz92f2_szBXx-6OfZvqzX5v8ivsB64PwDUf_kVKJrkAjWOfIjkOuPb8F__D22RgF0</recordid><startdate>20250216</startdate><enddate>20250216</enddate><creator>Fish, Shayla R.</creator><creator>Halley, Catherine L.</creator><creator>Dileepan, Mythili</creator><creator>Hertzel, Ann V.</creator><creator>Dickey, Deborah M.</creator><creator>Bernlohr, David A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0008-6032-6105</orcidid><orcidid>https://orcid.org/0000-0002-9172-7939</orcidid></search><sort><creationdate>20250216</creationdate><title>Expression of fatty acid binding proteins in mesenteric adipose tissue</title><author>Fish, Shayla R. ; Halley, Catherine L. ; Dileepan, Mythili ; Hertzel, Ann V. ; Dickey, Deborah M. ; Bernlohr, David A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1520-820dc4ea3df745155fa95824ab53516be8515b864df42f5dc8a8106187ac33e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Adipocytes - metabolism</topic><topic>Adipose Tissue - metabolism</topic><topic>Animals</topic><topic>Fatty Acid Binding Protein 3 - genetics</topic><topic>Fatty Acid Binding Protein 3 - metabolism</topic><topic>Fatty acid binding proteins</topic><topic>Fatty Acid-Binding Proteins - genetics</topic><topic>Fatty Acid-Binding Proteins - metabolism</topic><topic>Female</topic><topic>Lipid trafficking</topic><topic>Male</topic><topic>Mesentery</topic><topic>Mesentery - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Neoplasm Proteins</topic><topic>Visceral adipose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fish, Shayla R.</creatorcontrib><creatorcontrib>Halley, Catherine L.</creatorcontrib><creatorcontrib>Dileepan, Mythili</creatorcontrib><creatorcontrib>Hertzel, Ann V.</creatorcontrib><creatorcontrib>Dickey, Deborah M.</creatorcontrib><creatorcontrib>Bernlohr, David A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fish, Shayla R.</au><au>Halley, Catherine L.</au><au>Dileepan, Mythili</au><au>Hertzel, Ann V.</au><au>Dickey, Deborah M.</au><au>Bernlohr, David A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of fatty acid binding proteins in mesenteric adipose tissue</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2025-02-16</date><risdate>2025</risdate><volume>749</volume><spage>151346</spage><pages>151346-</pages><artnum>151346</artnum><issn>0006-291X</issn><issn>1090-2104</issn><eissn>1090-2104</eissn><abstract>Adipose is a complex tissue comprised of adipocytes, immune cells, endothelial and progenitor stem cells. In humans, there are at least nine defined adipose depots, each containing variable numbers of genetically identified adipocyte clusters suggesting remarkable heterogeneity and potential functionality in each depot with respect to lipid metabolism. Although subcutaneous and visceral depots are commonly analyzed for biochemical and molecular functions, the mesenteric depot has been overlooked yet strongly implicated in lipid mediated immune surveillance. Since fatty acid binding proteins (FABPs) are primary cellular conduits to lipid trafficking, we evaluated the expression patterns for four major fatty acid binding proteins (FABP1, FABP3, FABP4 and FABP5) using a combination of gene expression, immunoblotting, and immunofluorescence in mesenteric fat from both young and old, male and female C57Bl/6J mice. All four FABPs were expressed at the mRNA and protein level in murine mesenteric adipose tissue. While there was no statistical change in expression of mesenteric FABP isoforms with sex or age, the expression of mesenteric FABP1 was increased, and FABP4 decreased, in both males and females as compared to perigonadal and inguinal depots. Surprisingly, immunofluorescence staining revealed that compared to subcutaneous or perigonadal depots, mesenteric fat expresses FABP3, but little FABP5, in adipocytes. These results highlight the diversity in adipose tissue and the importance of evaluating the mesenteric depot in the context of lipid transport and metabolism. •FABP3 is expressed to high levels in mesenteric adipocytes.•FABP5 is expressed in the mesenteric stromal vascular fraction but undetectable in mesenteric adipocytes.•FABP1 expression is highest in mesenteric fat compared to FABP1 expression in inguinal or perigonadal fat.•FABP4 expression in highest expressing FABP in mesenteric adipose tissue.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>39855040</pmid><doi>10.1016/j.bbrc.2025.151346</doi><orcidid>https://orcid.org/0009-0008-6032-6105</orcidid><orcidid>https://orcid.org/0000-0002-9172-7939</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0006-291X
ispartof Biochemical and biophysical research communications, 2025-02, Vol.749, p.151346, Article 151346
issn 0006-291X
1090-2104
1090-2104
language eng
recordid cdi_proquest_miscellaneous_3159798291
source MEDLINE; Elsevier ScienceDirect Journals
subjects Adipocytes - metabolism
Adipose Tissue - metabolism
Animals
Fatty Acid Binding Protein 3 - genetics
Fatty Acid Binding Protein 3 - metabolism
Fatty acid binding proteins
Fatty Acid-Binding Proteins - genetics
Fatty Acid-Binding Proteins - metabolism
Female
Lipid trafficking
Male
Mesentery
Mesentery - metabolism
Mice
Mice, Inbred C57BL
Neoplasm Proteins
Visceral adipose
title Expression of fatty acid binding proteins in mesenteric adipose tissue
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T18%3A39%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20fatty%20acid%20binding%20proteins%20in%20mesenteric%20adipose%20tissue&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Fish,%20Shayla%20R.&rft.date=2025-02-16&rft.volume=749&rft.spage=151346&rft.pages=151346-&rft.artnum=151346&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2025.151346&rft_dat=%3Cproquest_cross%3E3159798291%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3159798291&rft_id=info:pmid/39855040&rft_els_id=S0006291X25000609&rfr_iscdi=true