Expression of fatty acid binding proteins in mesenteric adipose tissue
Adipose is a complex tissue comprised of adipocytes, immune cells, endothelial and progenitor stem cells. In humans, there are at least nine defined adipose depots, each containing variable numbers of genetically identified adipocyte clusters suggesting remarkable heterogeneity and potential functio...
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description | Adipose is a complex tissue comprised of adipocytes, immune cells, endothelial and progenitor stem cells. In humans, there are at least nine defined adipose depots, each containing variable numbers of genetically identified adipocyte clusters suggesting remarkable heterogeneity and potential functionality in each depot with respect to lipid metabolism. Although subcutaneous and visceral depots are commonly analyzed for biochemical and molecular functions, the mesenteric depot has been overlooked yet strongly implicated in lipid mediated immune surveillance. Since fatty acid binding proteins (FABPs) are primary cellular conduits to lipid trafficking, we evaluated the expression patterns for four major fatty acid binding proteins (FABP1, FABP3, FABP4 and FABP5) using a combination of gene expression, immunoblotting, and immunofluorescence in mesenteric fat from both young and old, male and female C57Bl/6J mice. All four FABPs were expressed at the mRNA and protein level in murine mesenteric adipose tissue. While there was no statistical change in expression of mesenteric FABP isoforms with sex or age, the expression of mesenteric FABP1 was increased, and FABP4 decreased, in both males and females as compared to perigonadal and inguinal depots. Surprisingly, immunofluorescence staining revealed that compared to subcutaneous or perigonadal depots, mesenteric fat expresses FABP3, but little FABP5, in adipocytes. These results highlight the diversity in adipose tissue and the importance of evaluating the mesenteric depot in the context of lipid transport and metabolism.
•FABP3 is expressed to high levels in mesenteric adipocytes.•FABP5 is expressed in the mesenteric stromal vascular fraction but undetectable in mesenteric adipocytes.•FABP1 expression is highest in mesenteric fat compared to FABP1 expression in inguinal or perigonadal fat.•FABP4 expression in highest expressing FABP in mesenteric adipose tissue. |
doi_str_mv | 10.1016/j.bbrc.2025.151346 |
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•FABP3 is expressed to high levels in mesenteric adipocytes.•FABP5 is expressed in the mesenteric stromal vascular fraction but undetectable in mesenteric adipocytes.•FABP1 expression is highest in mesenteric fat compared to FABP1 expression in inguinal or perigonadal fat.•FABP4 expression in highest expressing FABP in mesenteric adipose tissue.</description><identifier>ISSN: 0006-291X</identifier><identifier>ISSN: 1090-2104</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2025.151346</identifier><identifier>PMID: 39855040</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adipocytes - metabolism ; Adipose Tissue - metabolism ; Animals ; Fatty Acid Binding Protein 3 - genetics ; Fatty Acid Binding Protein 3 - metabolism ; Fatty acid binding proteins ; Fatty Acid-Binding Proteins - genetics ; Fatty Acid-Binding Proteins - metabolism ; Female ; Lipid trafficking ; Male ; Mesentery ; Mesentery - metabolism ; Mice ; Mice, Inbred C57BL ; Neoplasm Proteins ; Visceral adipose</subject><ispartof>Biochemical and biophysical research communications, 2025-02, Vol.749, p.151346, Article 151346</ispartof><rights>2025 Elsevier Inc.</rights><rights>Copyright © 2025 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1520-820dc4ea3df745155fa95824ab53516be8515b864df42f5dc8a8106187ac33e03</cites><orcidid>0009-0008-6032-6105 ; 0000-0002-9172-7939</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X25000609$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39855040$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fish, Shayla R.</creatorcontrib><creatorcontrib>Halley, Catherine L.</creatorcontrib><creatorcontrib>Dileepan, Mythili</creatorcontrib><creatorcontrib>Hertzel, Ann V.</creatorcontrib><creatorcontrib>Dickey, Deborah M.</creatorcontrib><creatorcontrib>Bernlohr, David A.</creatorcontrib><title>Expression of fatty acid binding proteins in mesenteric adipose tissue</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Adipose is a complex tissue comprised of adipocytes, immune cells, endothelial and progenitor stem cells. In humans, there are at least nine defined adipose depots, each containing variable numbers of genetically identified adipocyte clusters suggesting remarkable heterogeneity and potential functionality in each depot with respect to lipid metabolism. Although subcutaneous and visceral depots are commonly analyzed for biochemical and molecular functions, the mesenteric depot has been overlooked yet strongly implicated in lipid mediated immune surveillance. Since fatty acid binding proteins (FABPs) are primary cellular conduits to lipid trafficking, we evaluated the expression patterns for four major fatty acid binding proteins (FABP1, FABP3, FABP4 and FABP5) using a combination of gene expression, immunoblotting, and immunofluorescence in mesenteric fat from both young and old, male and female C57Bl/6J mice. All four FABPs were expressed at the mRNA and protein level in murine mesenteric adipose tissue. While there was no statistical change in expression of mesenteric FABP isoforms with sex or age, the expression of mesenteric FABP1 was increased, and FABP4 decreased, in both males and females as compared to perigonadal and inguinal depots. Surprisingly, immunofluorescence staining revealed that compared to subcutaneous or perigonadal depots, mesenteric fat expresses FABP3, but little FABP5, in adipocytes. These results highlight the diversity in adipose tissue and the importance of evaluating the mesenteric depot in the context of lipid transport and metabolism.
•FABP3 is expressed to high levels in mesenteric adipocytes.•FABP5 is expressed in the mesenteric stromal vascular fraction but undetectable in mesenteric adipocytes.•FABP1 expression is highest in mesenteric fat compared to FABP1 expression in inguinal or perigonadal fat.•FABP4 expression in highest expressing FABP in mesenteric adipose tissue.</description><subject>Adipocytes - metabolism</subject><subject>Adipose Tissue - metabolism</subject><subject>Animals</subject><subject>Fatty Acid Binding Protein 3 - genetics</subject><subject>Fatty Acid Binding Protein 3 - metabolism</subject><subject>Fatty acid binding proteins</subject><subject>Fatty Acid-Binding Proteins - genetics</subject><subject>Fatty Acid-Binding Proteins - metabolism</subject><subject>Female</subject><subject>Lipid trafficking</subject><subject>Male</subject><subject>Mesentery</subject><subject>Mesentery - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Neoplasm Proteins</subject><subject>Visceral adipose</subject><issn>0006-291X</issn><issn>1090-2104</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFKAzEQhoMotlZfwIPk6GXrTDbZZsGLlFaFghcFbyGbzEpKu1uTrdi3d0urR08Dw_f_zHyMXSOMEbC4W46rKrqxAKHGqDCXxQkbIpSQCQR5yoYAUGSixPcBu0hpCYAoi_KcDfJSKwUShmw--95ESim0DW9rXtuu23HrgudVaHxoPvgmth2FJvHQ8DUlajqKwXHrw6ZNxLuQ0pYu2VltV4mujnPE3uaz1-lTtnh5fJ4-LDKHSkCmBXgnyea-nkiFStW2VFpIW6lcYVGR7peVLqSvpaiVd9pqhAL1xLo8J8hH7PbQ21_1uaXUmXVIjlYr21C7TSZHVU5K3f_co-KAutimFKk2mxjWNu4Mgtn7M0uz92f2_szBXx-6OfZvqzX5v8ivsB64PwDUf_kVKJrkAjWOfIjkOuPb8F__D22RgF0</recordid><startdate>20250216</startdate><enddate>20250216</enddate><creator>Fish, Shayla R.</creator><creator>Halley, Catherine L.</creator><creator>Dileepan, Mythili</creator><creator>Hertzel, Ann V.</creator><creator>Dickey, Deborah M.</creator><creator>Bernlohr, David A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0008-6032-6105</orcidid><orcidid>https://orcid.org/0000-0002-9172-7939</orcidid></search><sort><creationdate>20250216</creationdate><title>Expression of fatty acid binding proteins in mesenteric adipose tissue</title><author>Fish, Shayla R. ; Halley, Catherine L. ; Dileepan, Mythili ; Hertzel, Ann V. ; Dickey, Deborah M. ; Bernlohr, David A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1520-820dc4ea3df745155fa95824ab53516be8515b864df42f5dc8a8106187ac33e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Adipocytes - metabolism</topic><topic>Adipose Tissue - metabolism</topic><topic>Animals</topic><topic>Fatty Acid Binding Protein 3 - genetics</topic><topic>Fatty Acid Binding Protein 3 - metabolism</topic><topic>Fatty acid binding proteins</topic><topic>Fatty Acid-Binding Proteins - genetics</topic><topic>Fatty Acid-Binding Proteins - metabolism</topic><topic>Female</topic><topic>Lipid trafficking</topic><topic>Male</topic><topic>Mesentery</topic><topic>Mesentery - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Neoplasm Proteins</topic><topic>Visceral adipose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fish, Shayla R.</creatorcontrib><creatorcontrib>Halley, Catherine L.</creatorcontrib><creatorcontrib>Dileepan, Mythili</creatorcontrib><creatorcontrib>Hertzel, Ann V.</creatorcontrib><creatorcontrib>Dickey, Deborah M.</creatorcontrib><creatorcontrib>Bernlohr, David A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fish, Shayla R.</au><au>Halley, Catherine L.</au><au>Dileepan, Mythili</au><au>Hertzel, Ann V.</au><au>Dickey, Deborah M.</au><au>Bernlohr, David A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of fatty acid binding proteins in mesenteric adipose tissue</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2025-02-16</date><risdate>2025</risdate><volume>749</volume><spage>151346</spage><pages>151346-</pages><artnum>151346</artnum><issn>0006-291X</issn><issn>1090-2104</issn><eissn>1090-2104</eissn><abstract>Adipose is a complex tissue comprised of adipocytes, immune cells, endothelial and progenitor stem cells. In humans, there are at least nine defined adipose depots, each containing variable numbers of genetically identified adipocyte clusters suggesting remarkable heterogeneity and potential functionality in each depot with respect to lipid metabolism. Although subcutaneous and visceral depots are commonly analyzed for biochemical and molecular functions, the mesenteric depot has been overlooked yet strongly implicated in lipid mediated immune surveillance. Since fatty acid binding proteins (FABPs) are primary cellular conduits to lipid trafficking, we evaluated the expression patterns for four major fatty acid binding proteins (FABP1, FABP3, FABP4 and FABP5) using a combination of gene expression, immunoblotting, and immunofluorescence in mesenteric fat from both young and old, male and female C57Bl/6J mice. All four FABPs were expressed at the mRNA and protein level in murine mesenteric adipose tissue. While there was no statistical change in expression of mesenteric FABP isoforms with sex or age, the expression of mesenteric FABP1 was increased, and FABP4 decreased, in both males and females as compared to perigonadal and inguinal depots. Surprisingly, immunofluorescence staining revealed that compared to subcutaneous or perigonadal depots, mesenteric fat expresses FABP3, but little FABP5, in adipocytes. These results highlight the diversity in adipose tissue and the importance of evaluating the mesenteric depot in the context of lipid transport and metabolism.
•FABP3 is expressed to high levels in mesenteric adipocytes.•FABP5 is expressed in the mesenteric stromal vascular fraction but undetectable in mesenteric adipocytes.•FABP1 expression is highest in mesenteric fat compared to FABP1 expression in inguinal or perigonadal fat.•FABP4 expression in highest expressing FABP in mesenteric adipose tissue.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>39855040</pmid><doi>10.1016/j.bbrc.2025.151346</doi><orcidid>https://orcid.org/0009-0008-6032-6105</orcidid><orcidid>https://orcid.org/0000-0002-9172-7939</orcidid></addata></record> |
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subjects | Adipocytes - metabolism Adipose Tissue - metabolism Animals Fatty Acid Binding Protein 3 - genetics Fatty Acid Binding Protein 3 - metabolism Fatty acid binding proteins Fatty Acid-Binding Proteins - genetics Fatty Acid-Binding Proteins - metabolism Female Lipid trafficking Male Mesentery Mesentery - metabolism Mice Mice, Inbred C57BL Neoplasm Proteins Visceral adipose |
title | Expression of fatty acid binding proteins in mesenteric adipose tissue |
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