Anti-TMV Activity and Functional Mechanisms of Two Diterpenoid Alkaloids Isolated from Dendrobium findlayanum
Tobacco mosaic virus (TMV) is a major threat to crops, making the discovery of green biopesticides essential. Herein, we present two active ingredients derived from the medicinal plant Dendrobium findlayanum, findlayine A (1) and dendrofindline B (2), as promising precursor compounds for TMV inhibit...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2025-01, Vol.73 (4), p.2443-2450 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Song, Cheng-Gang Yang, Yang Cui, Wenjing Chen, Wei Wang, Shouxian Qin, Jianchun Liu, Jinliang |
| description | Tobacco mosaic virus (TMV) is a major threat to crops, making the discovery of green biopesticides essential. Herein, we present two active ingredients derived from the medicinal plant Dendrobium findlayanum, findlayine A (1) and dendrofindline B (2), as promising precursor compounds for TMV inhibitors. Among them, 2 inhibited TMV infestation on tobacco leaves at a rate of 38.6%, which was close to that of the commercial antiviral agent ningnanmycin (43.1%). Both 1 and 2 could effectively alleviate the destruction of tobacco leaf protoplasts by TMV infestation, and enhance the resistance of tobacco to TMV by affecting the activities of phenylalaninammo-nialyase (PAL), peroxidase (POD) and superoxide dismutase (SOD). Additionally, 1 and 2 significantly down-regulated the expression of TMV coat protein (CP) and inhibited CP-mediated aggregation of TMV, thereby reducing its infestation capacity. This study systematically investigated the mechanism of anti-TMV activity of diterpenoid alkaloids from D. findlayanum, with a view to providing new insights into the creation of novel green antiviral agents. |
| doi_str_mv | 10.1021/acs.jafc.4c12988 |
| format | Article |
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Herein, we present two active ingredients derived from the medicinal plant Dendrobium findlayanum, findlayine A (1) and dendrofindline B (2), as promising precursor compounds for TMV inhibitors. Among them, 2 inhibited TMV infestation on tobacco leaves at a rate of 38.6%, which was close to that of the commercial antiviral agent ningnanmycin (43.1%). Both 1 and 2 could effectively alleviate the destruction of tobacco leaf protoplasts by TMV infestation, and enhance the resistance of tobacco to TMV by affecting the activities of phenylalaninammo-nialyase (PAL), peroxidase (POD) and superoxide dismutase (SOD). Additionally, 1 and 2 significantly down-regulated the expression of TMV coat protein (CP) and inhibited CP-mediated aggregation of TMV, thereby reducing its infestation capacity. This study systematically investigated the mechanism of anti-TMV activity of diterpenoid alkaloids from D. findlayanum, with a view to providing new insights into the creation of novel green antiviral agents.</description><identifier>ISSN: 0021-8561</identifier><identifier>ISSN: 1520-5118</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.4c12988</identifier><identifier>PMID: 39810463</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Alkaloids - chemistry ; Alkaloids - pharmacology ; Antiviral Agents - chemistry ; Antiviral Agents - pharmacology ; Bioactive Constituents, Metabolites, and Functions ; Dendrobium - chemistry ; Diterpenes - chemistry ; Diterpenes - pharmacology ; Nicotiana - chemistry ; Nicotiana - parasitology ; Plant Diseases - parasitology ; Plant Diseases - virology ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Superoxide Dismutase - metabolism ; Tobacco Mosaic Virus - drug effects ; Tobacco Mosaic Virus - physiology</subject><ispartof>Journal of agricultural and food chemistry, 2025-01, Vol.73 (4), p.2443-2450</ispartof><rights>2025 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a219t-dbf0c8c650187115e36cff6867871b449485f8813a2769aa81148d09eb37fbd03</cites><orcidid>0000-0002-5903-2272</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.4c12988$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.4c12988$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39810463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Cheng-Gang</creatorcontrib><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Cui, Wenjing</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><creatorcontrib>Wang, Shouxian</creatorcontrib><creatorcontrib>Qin, Jianchun</creatorcontrib><creatorcontrib>Liu, Jinliang</creatorcontrib><title>Anti-TMV Activity and Functional Mechanisms of Two Diterpenoid Alkaloids Isolated from Dendrobium findlayanum</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Tobacco mosaic virus (TMV) is a major threat to crops, making the discovery of green biopesticides essential. Herein, we present two active ingredients derived from the medicinal plant Dendrobium findlayanum, findlayine A (1) and dendrofindline B (2), as promising precursor compounds for TMV inhibitors. Among them, 2 inhibited TMV infestation on tobacco leaves at a rate of 38.6%, which was close to that of the commercial antiviral agent ningnanmycin (43.1%). Both 1 and 2 could effectively alleviate the destruction of tobacco leaf protoplasts by TMV infestation, and enhance the resistance of tobacco to TMV by affecting the activities of phenylalaninammo-nialyase (PAL), peroxidase (POD) and superoxide dismutase (SOD). Additionally, 1 and 2 significantly down-regulated the expression of TMV coat protein (CP) and inhibited CP-mediated aggregation of TMV, thereby reducing its infestation capacity. This study systematically investigated the mechanism of anti-TMV activity of diterpenoid alkaloids from D. findlayanum, with a view to providing new insights into the creation of novel green antiviral agents.</description><subject>Alkaloids - chemistry</subject><subject>Alkaloids - pharmacology</subject><subject>Antiviral Agents - chemistry</subject><subject>Antiviral Agents - pharmacology</subject><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>Dendrobium - chemistry</subject><subject>Diterpenes - chemistry</subject><subject>Diterpenes - pharmacology</subject><subject>Nicotiana - chemistry</subject><subject>Nicotiana - parasitology</subject><subject>Plant Diseases - parasitology</subject><subject>Plant Diseases - virology</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Tobacco Mosaic Virus - drug effects</subject><subject>Tobacco Mosaic Virus - physiology</subject><issn>0021-8561</issn><issn>1520-5118</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDtPwzAUhS0EglLYmZBHBlJ8kzhxxqrlJbViKayR44cwJHaxE1D_PS4tbEz3Huk7Z_gQugAyAZLCDRdh8sa1mOQC0oqxAzQCmpKEArBDNCKRSRgt4ASdhvBGCGG0JMfoJKsYkLzIRqib2t4kq-ULnorefJp-g7mV-G6wMTrLW7xU4pVbE7qAncarL4fnpld-rawzEk_bd97GJ-DH4FreK4m1dx2eKyu9a8zQYW2sbPmG26E7Q0eat0Gd7-8YPd_drmYPyeLp_nE2XSQ8hapPZKOJYKKgBFgJQFVWCK0LVpQxNnle5YxqxiDjaVlUnDOAnElSqSYrdSNJNkZXu921dx-DCn3dmSBU23Kr3BDqDCgtgaWsjCjZocK7ELzS9dqbjvtNDaTeSq6j5Horud5LjpXL_frQdEr-FX6tRuB6B_xU3eCjx_D_3jcGyYis</recordid><startdate>20250129</startdate><enddate>20250129</enddate><creator>Song, Cheng-Gang</creator><creator>Yang, Yang</creator><creator>Cui, Wenjing</creator><creator>Chen, Wei</creator><creator>Wang, Shouxian</creator><creator>Qin, Jianchun</creator><creator>Liu, Jinliang</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5903-2272</orcidid></search><sort><creationdate>20250129</creationdate><title>Anti-TMV Activity and Functional Mechanisms of Two Diterpenoid Alkaloids Isolated from Dendrobium findlayanum</title><author>Song, Cheng-Gang ; Yang, Yang ; Cui, Wenjing ; Chen, Wei ; Wang, Shouxian ; Qin, Jianchun ; Liu, Jinliang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a219t-dbf0c8c650187115e36cff6867871b449485f8813a2769aa81148d09eb37fbd03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Alkaloids - chemistry</topic><topic>Alkaloids - pharmacology</topic><topic>Antiviral Agents - chemistry</topic><topic>Antiviral Agents - pharmacology</topic><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>Dendrobium - chemistry</topic><topic>Diterpenes - chemistry</topic><topic>Diterpenes - pharmacology</topic><topic>Nicotiana - chemistry</topic><topic>Nicotiana - parasitology</topic><topic>Plant Diseases - parasitology</topic><topic>Plant Diseases - virology</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Tobacco Mosaic Virus - drug effects</topic><topic>Tobacco Mosaic Virus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Cheng-Gang</creatorcontrib><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Cui, Wenjing</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><creatorcontrib>Wang, Shouxian</creatorcontrib><creatorcontrib>Qin, Jianchun</creatorcontrib><creatorcontrib>Liu, Jinliang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Cheng-Gang</au><au>Yang, Yang</au><au>Cui, Wenjing</au><au>Chen, Wei</au><au>Wang, Shouxian</au><au>Qin, Jianchun</au><au>Liu, Jinliang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-TMV Activity and Functional Mechanisms of Two Diterpenoid Alkaloids Isolated from Dendrobium findlayanum</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2025-01-29</date><risdate>2025</risdate><volume>73</volume><issue>4</issue><spage>2443</spage><epage>2450</epage><pages>2443-2450</pages><issn>0021-8561</issn><issn>1520-5118</issn><eissn>1520-5118</eissn><abstract>Tobacco mosaic virus (TMV) is a major threat to crops, making the discovery of green biopesticides essential. Herein, we present two active ingredients derived from the medicinal plant Dendrobium findlayanum, findlayine A (1) and dendrofindline B (2), as promising precursor compounds for TMV inhibitors. Among them, 2 inhibited TMV infestation on tobacco leaves at a rate of 38.6%, which was close to that of the commercial antiviral agent ningnanmycin (43.1%). Both 1 and 2 could effectively alleviate the destruction of tobacco leaf protoplasts by TMV infestation, and enhance the resistance of tobacco to TMV by affecting the activities of phenylalaninammo-nialyase (PAL), peroxidase (POD) and superoxide dismutase (SOD). Additionally, 1 and 2 significantly down-regulated the expression of TMV coat protein (CP) and inhibited CP-mediated aggregation of TMV, thereby reducing its infestation capacity. This study systematically investigated the mechanism of anti-TMV activity of diterpenoid alkaloids from D. findlayanum, with a view to providing new insights into the creation of novel green antiviral agents.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>39810463</pmid><doi>10.1021/acs.jafc.4c12988</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-5903-2272</orcidid></addata></record> |
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| subjects | Alkaloids - chemistry Alkaloids - pharmacology Antiviral Agents - chemistry Antiviral Agents - pharmacology Bioactive Constituents, Metabolites, and Functions Dendrobium - chemistry Diterpenes - chemistry Diterpenes - pharmacology Nicotiana - chemistry Nicotiana - parasitology Plant Diseases - parasitology Plant Diseases - virology Plant Extracts - chemistry Plant Extracts - pharmacology Plant Leaves - chemistry Superoxide Dismutase - metabolism Tobacco Mosaic Virus - drug effects Tobacco Mosaic Virus - physiology |
| title | Anti-TMV Activity and Functional Mechanisms of Two Diterpenoid Alkaloids Isolated from Dendrobium findlayanum |
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