Stepwise processing of Chlorella sorokiniana confers plant biostimulant that reduces mineral fertilizer requirements
[Display omitted] •Stepwise processing rendered algal fractions with various biostimulant activity.•A blend of fractions enhanced C and N metabolism in tomato plants.•Application of the blend decreased mineral fertilizer use by 25 %.•The method results in ample residual biomass for a future biorefin...
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| Veröffentlicht in: | Bioresource technology 2025-02, Vol.418, p.131923, Article 131923 |
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| creator | Chovancek, Erik Poque, Sylvain Bayram, Engin Borhan, Emren Jokel, Martina Rantanen, Iida-Maria Haznedaroglu, Berat Z. Himanen, Kristiina Sirin, Sema Allahverdiyeva, Yagut |
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•Stepwise processing rendered algal fractions with various biostimulant activity.•A blend of fractions enhanced C and N metabolism in tomato plants.•Application of the blend decreased mineral fertilizer use by 25 %.•The method results in ample residual biomass for a future biorefinery approach.
We developed a stepwise method to transform Chlorella sorokiniana microalgal biomass into a potent biostimulant. The method, including maceration, high-pressure homogenization, and enzymatic hydrolysis, preserves the bioactive properties of the biomass as a biostimulant while minimizing plant inhibitory effects. Fractions were characterized individually, and optimal concentrations were determined using a rapid Arabidopsis root assay. A blend of optimal concentrations of fractions was identified as the most stimulating extract, increasing the root elongation by 25 %. When applied to tomato plants and monitored using high-throughput plant phenotyping, the blend displayed a 25 % reduction in mineral fertilizer use. Metabolomic analysis of the tomato plants showed significantly enhanced carbon and nitrogen metabolism in the leaves. Our findings indicate that the stepwise processing not only produces an effective biostimulant but also generates substantial residual biomass for a potential multiproduct biorefinery approach that can improve the overall techno-economic outlook. |
| doi_str_mv | 10.1016/j.biortech.2024.131923 |
| format | Article |
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•Stepwise processing rendered algal fractions with various biostimulant activity.•A blend of fractions enhanced C and N metabolism in tomato plants.•Application of the blend decreased mineral fertilizer use by 25 %.•The method results in ample residual biomass for a future biorefinery approach.
We developed a stepwise method to transform Chlorella sorokiniana microalgal biomass into a potent biostimulant. The method, including maceration, high-pressure homogenization, and enzymatic hydrolysis, preserves the bioactive properties of the biomass as a biostimulant while minimizing plant inhibitory effects. Fractions were characterized individually, and optimal concentrations were determined using a rapid Arabidopsis root assay. A blend of optimal concentrations of fractions was identified as the most stimulating extract, increasing the root elongation by 25 %. When applied to tomato plants and monitored using high-throughput plant phenotyping, the blend displayed a 25 % reduction in mineral fertilizer use. Metabolomic analysis of the tomato plants showed significantly enhanced carbon and nitrogen metabolism in the leaves. Our findings indicate that the stepwise processing not only produces an effective biostimulant but also generates substantial residual biomass for a potential multiproduct biorefinery approach that can improve the overall techno-economic outlook.</description><identifier>ISSN: 0960-8524</identifier><identifier>ISSN: 1873-2976</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2024.131923</identifier><identifier>PMID: 39626806</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Arabidopsis ; biomass ; biorefining ; carbon ; Chlorella sorokiniana ; Enzymatic hydrolysis ; fertilizer application ; High-pressure homogenization ; homogenization ; Maceration ; metabolomics ; Microalgae ; mineral fertilizers ; nitrogen metabolism ; phenotype ; Plant phenotyping ; root growth ; Tomato ; tomatoes</subject><ispartof>Bioresource technology, 2025-02, Vol.418, p.131923, Article 131923</ispartof><rights>2024 The Authors</rights><rights>Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c278t-cdb4dc5fc062acb3bb777149f02cad7412c1b80407106cc31dc574f6682776ec3</cites><orcidid>0000-0001-9195-7577 ; 0009-0002-9913-4422 ; 0000-0002-9262-1757 ; 0000-0003-4575-8395 ; 0000-0002-0081-8801 ; 0000-0002-2695-8052 ; 0000-0003-4975-9467 ; 0000-0002-5368-0738 ; 0000-0001-8332-6695</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960852424016274$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39626806$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chovancek, Erik</creatorcontrib><creatorcontrib>Poque, Sylvain</creatorcontrib><creatorcontrib>Bayram, Engin</creatorcontrib><creatorcontrib>Borhan, Emren</creatorcontrib><creatorcontrib>Jokel, Martina</creatorcontrib><creatorcontrib>Rantanen, Iida-Maria</creatorcontrib><creatorcontrib>Haznedaroglu, Berat Z.</creatorcontrib><creatorcontrib>Himanen, Kristiina</creatorcontrib><creatorcontrib>Sirin, Sema</creatorcontrib><creatorcontrib>Allahverdiyeva, Yagut</creatorcontrib><title>Stepwise processing of Chlorella sorokiniana confers plant biostimulant that reduces mineral fertilizer requirements</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>[Display omitted]
•Stepwise processing rendered algal fractions with various biostimulant activity.•A blend of fractions enhanced C and N metabolism in tomato plants.•Application of the blend decreased mineral fertilizer use by 25 %.•The method results in ample residual biomass for a future biorefinery approach.
We developed a stepwise method to transform Chlorella sorokiniana microalgal biomass into a potent biostimulant. The method, including maceration, high-pressure homogenization, and enzymatic hydrolysis, preserves the bioactive properties of the biomass as a biostimulant while minimizing plant inhibitory effects. Fractions were characterized individually, and optimal concentrations were determined using a rapid Arabidopsis root assay. A blend of optimal concentrations of fractions was identified as the most stimulating extract, increasing the root elongation by 25 %. When applied to tomato plants and monitored using high-throughput plant phenotyping, the blend displayed a 25 % reduction in mineral fertilizer use. Metabolomic analysis of the tomato plants showed significantly enhanced carbon and nitrogen metabolism in the leaves. Our findings indicate that the stepwise processing not only produces an effective biostimulant but also generates substantial residual biomass for a potential multiproduct biorefinery approach that can improve the overall techno-economic outlook.</description><subject>Arabidopsis</subject><subject>biomass</subject><subject>biorefining</subject><subject>carbon</subject><subject>Chlorella sorokiniana</subject><subject>Enzymatic hydrolysis</subject><subject>fertilizer application</subject><subject>High-pressure homogenization</subject><subject>homogenization</subject><subject>Maceration</subject><subject>metabolomics</subject><subject>Microalgae</subject><subject>mineral fertilizers</subject><subject>nitrogen metabolism</subject><subject>phenotype</subject><subject>Plant phenotyping</subject><subject>root growth</subject><subject>Tomato</subject><subject>tomatoes</subject><issn>0960-8524</issn><issn>1873-2976</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><recordid>eNqNkU1v1DAQhi1ERZfCX6h85JKtv9ZObqAVBaRKHNqeLWcyYb0k9tZ2qODX42VbrnCyLD_zjmceQi45W3PG9dV-3fuYCsJuLZhQay55J-QLsuKtkY3ojH5JVqzTrGk3Qp2T1znvGWOSG_GKnMtOC90yvSLltuDh0WekhxQBc_bhG40j3e6mmHCaHM0xxe8-eBcchRhGTJkeJhcKrT_Ixc_Ln0vZuUITDksNobMPmNxEK1z85H9hqk8Pi084Yyj5DTkb3ZTx7dN5Qe6vP95tPzc3Xz992X64aUCYtjQw9GqAzQhMCwe97HtjDFfdyAS4wSgugPctU8xwpgEkr7BRo9atMEYjyAvy7pRbZ3tYMBc7-wzHqQLGJVvJN0poJbX4D1SxrsbyI6pPKKSYc8LRHpKfXfppObNHOXZvn-XYoxx7klMLL596LP2Mw9-yZxsVeH8CsC7lh8dkM3gMgEPdHBQ7RP-vHr8BS6CmoQ</recordid><startdate>20250201</startdate><enddate>20250201</enddate><creator>Chovancek, Erik</creator><creator>Poque, Sylvain</creator><creator>Bayram, Engin</creator><creator>Borhan, Emren</creator><creator>Jokel, Martina</creator><creator>Rantanen, Iida-Maria</creator><creator>Haznedaroglu, Berat Z.</creator><creator>Himanen, Kristiina</creator><creator>Sirin, Sema</creator><creator>Allahverdiyeva, Yagut</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-9195-7577</orcidid><orcidid>https://orcid.org/0009-0002-9913-4422</orcidid><orcidid>https://orcid.org/0000-0002-9262-1757</orcidid><orcidid>https://orcid.org/0000-0003-4575-8395</orcidid><orcidid>https://orcid.org/0000-0002-0081-8801</orcidid><orcidid>https://orcid.org/0000-0002-2695-8052</orcidid><orcidid>https://orcid.org/0000-0003-4975-9467</orcidid><orcidid>https://orcid.org/0000-0002-5368-0738</orcidid><orcidid>https://orcid.org/0000-0001-8332-6695</orcidid></search><sort><creationdate>20250201</creationdate><title>Stepwise processing of Chlorella sorokiniana confers plant biostimulant that reduces mineral fertilizer requirements</title><author>Chovancek, Erik ; Poque, Sylvain ; Bayram, Engin ; Borhan, Emren ; Jokel, Martina ; Rantanen, Iida-Maria ; Haznedaroglu, Berat Z. ; Himanen, Kristiina ; Sirin, Sema ; Allahverdiyeva, Yagut</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c278t-cdb4dc5fc062acb3bb777149f02cad7412c1b80407106cc31dc574f6682776ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Arabidopsis</topic><topic>biomass</topic><topic>biorefining</topic><topic>carbon</topic><topic>Chlorella sorokiniana</topic><topic>Enzymatic hydrolysis</topic><topic>fertilizer application</topic><topic>High-pressure homogenization</topic><topic>homogenization</topic><topic>Maceration</topic><topic>metabolomics</topic><topic>Microalgae</topic><topic>mineral fertilizers</topic><topic>nitrogen metabolism</topic><topic>phenotype</topic><topic>Plant phenotyping</topic><topic>root growth</topic><topic>Tomato</topic><topic>tomatoes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chovancek, Erik</creatorcontrib><creatorcontrib>Poque, Sylvain</creatorcontrib><creatorcontrib>Bayram, Engin</creatorcontrib><creatorcontrib>Borhan, Emren</creatorcontrib><creatorcontrib>Jokel, Martina</creatorcontrib><creatorcontrib>Rantanen, Iida-Maria</creatorcontrib><creatorcontrib>Haznedaroglu, Berat Z.</creatorcontrib><creatorcontrib>Himanen, Kristiina</creatorcontrib><creatorcontrib>Sirin, Sema</creatorcontrib><creatorcontrib>Allahverdiyeva, Yagut</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chovancek, Erik</au><au>Poque, Sylvain</au><au>Bayram, Engin</au><au>Borhan, Emren</au><au>Jokel, Martina</au><au>Rantanen, Iida-Maria</au><au>Haznedaroglu, Berat Z.</au><au>Himanen, Kristiina</au><au>Sirin, Sema</au><au>Allahverdiyeva, Yagut</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stepwise processing of Chlorella sorokiniana confers plant biostimulant that reduces mineral fertilizer requirements</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2025-02-01</date><risdate>2025</risdate><volume>418</volume><spage>131923</spage><pages>131923-</pages><artnum>131923</artnum><issn>0960-8524</issn><issn>1873-2976</issn><eissn>1873-2976</eissn><abstract>[Display omitted]
•Stepwise processing rendered algal fractions with various biostimulant activity.•A blend of fractions enhanced C and N metabolism in tomato plants.•Application of the blend decreased mineral fertilizer use by 25 %.•The method results in ample residual biomass for a future biorefinery approach.
We developed a stepwise method to transform Chlorella sorokiniana microalgal biomass into a potent biostimulant. The method, including maceration, high-pressure homogenization, and enzymatic hydrolysis, preserves the bioactive properties of the biomass as a biostimulant while minimizing plant inhibitory effects. Fractions were characterized individually, and optimal concentrations were determined using a rapid Arabidopsis root assay. A blend of optimal concentrations of fractions was identified as the most stimulating extract, increasing the root elongation by 25 %. When applied to tomato plants and monitored using high-throughput plant phenotyping, the blend displayed a 25 % reduction in mineral fertilizer use. Metabolomic analysis of the tomato plants showed significantly enhanced carbon and nitrogen metabolism in the leaves. Our findings indicate that the stepwise processing not only produces an effective biostimulant but also generates substantial residual biomass for a potential multiproduct biorefinery approach that can improve the overall techno-economic outlook.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>39626806</pmid><doi>10.1016/j.biortech.2024.131923</doi><orcidid>https://orcid.org/0000-0001-9195-7577</orcidid><orcidid>https://orcid.org/0009-0002-9913-4422</orcidid><orcidid>https://orcid.org/0000-0002-9262-1757</orcidid><orcidid>https://orcid.org/0000-0003-4575-8395</orcidid><orcidid>https://orcid.org/0000-0002-0081-8801</orcidid><orcidid>https://orcid.org/0000-0002-2695-8052</orcidid><orcidid>https://orcid.org/0000-0003-4975-9467</orcidid><orcidid>https://orcid.org/0000-0002-5368-0738</orcidid><orcidid>https://orcid.org/0000-0001-8332-6695</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Arabidopsis biomass biorefining carbon Chlorella sorokiniana Enzymatic hydrolysis fertilizer application High-pressure homogenization homogenization Maceration metabolomics Microalgae mineral fertilizers nitrogen metabolism phenotype Plant phenotyping root growth Tomato tomatoes |
| title | Stepwise processing of Chlorella sorokiniana confers plant biostimulant that reduces mineral fertilizer requirements |
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