Structural and Genetic Relationship of the O-Antigens of the Type Strains Azospirillum agricola CC-HIH038 and Azospirillum doebereinerae GSF71
A lipopolysaccharide was isolated from cells of the type strain of a rhizobacterium Azospirillum agricola CC-HIH038 by phenol extraction. The O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide followed by chromatographic fractionation. Monosaccharide analysis, i...
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| Veröffentlicht in: | Microbiology (New York) 2024-12, Vol.93 (6), p.855-863 |
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| creator | Sigida, E. N. Grinev, V. S. Kokoulin, M. S. Konnova, S. A. Fedonenko, Y. P. |
| description | A lipopolysaccharide was isolated from cells of the type strain of a rhizobacterium Azospirillum agricola CC-HIH038 by phenol extraction. The O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide followed by chromatographic fractionation. Monosaccharide analysis, including determination of absolute configurations and 1D and 2D ¹H and ¹³C NMR spectroscopy, revealed the following structure of the O-specific polysaccharide repeating unit of A. agricola CC-HIH038ᵀ: →3)-α-L-Rhap2Ac-(1 → 3)-α-L-Rhap-(1 → 3)-α-L-Rhap-(1 → 3)-β-D-GlcpNAc6Ac-(1→. This unit is structurally related to that of A. doebereinerae GSF71ᵀ. Based on the analysis of full-genome sequencing data for strains A. agricola CC-HIH038ᵀ and A. doebereinerae GSF71ᵀ, the loci for O-specific polysaccharide biosynthesis have been identified. They were characterized by similar organization and a high level of gene homology, confirming the common structure of the O-antigens of these strains. |
| doi_str_mv | 10.1134/S0026261724606948 |
| format | Article |
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N. ; Grinev, V. S. ; Kokoulin, M. S. ; Konnova, S. A. ; Fedonenko, Y. P.</creator><creatorcontrib>Sigida, E. N. ; Grinev, V. S. ; Kokoulin, M. S. ; Konnova, S. A. ; Fedonenko, Y. P.</creatorcontrib><description>A lipopolysaccharide was isolated from cells of the type strain of a rhizobacterium Azospirillum agricola CC-HIH038 by phenol extraction. The O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide followed by chromatographic fractionation. Monosaccharide analysis, including determination of absolute configurations and 1D and 2D ¹H and ¹³C NMR spectroscopy, revealed the following structure of the O-specific polysaccharide repeating unit of A. agricola CC-HIH038ᵀ: →3)-α-L-Rhap2Ac-(1 → 3)-α-L-Rhap-(1 → 3)-α-L-Rhap-(1 → 3)-β-D-GlcpNAc6Ac-(1→. This unit is structurally related to that of A. doebereinerae GSF71ᵀ. Based on the analysis of full-genome sequencing data for strains A. agricola CC-HIH038ᵀ and A. doebereinerae GSF71ᵀ, the loci for O-specific polysaccharide biosynthesis have been identified. They were characterized by similar organization and a high level of gene homology, confirming the common structure of the O-antigens of these strains.</description><identifier>ISSN: 0026-2617</identifier><identifier>EISSN: 1608-3237</identifier><identifier>DOI: 10.1134/S0026261724606948</identifier><language>eng</language><subject>acid hydrolysis ; Azospirillum doebereinerae ; biosynthesis ; chromatography ; fractionation ; genes ; genetic relationships ; microbiology ; nuclear magnetic resonance spectroscopy ; O-antigens ; phenol</subject><ispartof>Microbiology (New York), 2024-12, Vol.93 (6), p.855-863</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c758-50ab8e41c23fce9edc218c81a031b4d29736d1b9c22439473c3487e7098b44463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Sigida, E. N.</creatorcontrib><creatorcontrib>Grinev, V. S.</creatorcontrib><creatorcontrib>Kokoulin, M. S.</creatorcontrib><creatorcontrib>Konnova, S. A.</creatorcontrib><creatorcontrib>Fedonenko, Y. P.</creatorcontrib><title>Structural and Genetic Relationship of the O-Antigens of the Type Strains Azospirillum agricola CC-HIH038 and Azospirillum doebereinerae GSF71</title><title>Microbiology (New York)</title><description>A lipopolysaccharide was isolated from cells of the type strain of a rhizobacterium Azospirillum agricola CC-HIH038 by phenol extraction. The O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide followed by chromatographic fractionation. Monosaccharide analysis, including determination of absolute configurations and 1D and 2D ¹H and ¹³C NMR spectroscopy, revealed the following structure of the O-specific polysaccharide repeating unit of A. agricola CC-HIH038ᵀ: →3)-α-L-Rhap2Ac-(1 → 3)-α-L-Rhap-(1 → 3)-α-L-Rhap-(1 → 3)-β-D-GlcpNAc6Ac-(1→. This unit is structurally related to that of A. doebereinerae GSF71ᵀ. Based on the analysis of full-genome sequencing data for strains A. agricola CC-HIH038ᵀ and A. doebereinerae GSF71ᵀ, the loci for O-specific polysaccharide biosynthesis have been identified. They were characterized by similar organization and a high level of gene homology, confirming the common structure of the O-antigens of these strains.</description><subject>acid hydrolysis</subject><subject>Azospirillum doebereinerae</subject><subject>biosynthesis</subject><subject>chromatography</subject><subject>fractionation</subject><subject>genes</subject><subject>genetic relationships</subject><subject>microbiology</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>O-antigens</subject><subject>phenol</subject><issn>0026-2617</issn><issn>1608-3237</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNplkLtOwzAYhS0EEqXwAGweWQK-JXbGKqIXCakS7R45zp_WyI2DnQzlIXhmUgoDYjrSOZ--4SB0T8kjpVw8bQhhGcuoZCIjWS7UBZrQjKiEMy4v0eQ0J6f9Gt3E-EYISVmaTtDnpg-D6YegHdZtjRfQQm8NfgWne-vbuLcd9g3u94DXyazt7Q7a-Ntsjx3g0aDt2M0-fOxssM4NB6x3wRrvNC6KZLlaEq6-9X-Y2kMFAWwLQQNebOaS3qKrRrsIdz85Rdv587ZYJi_rxaqYvSRGpipJia4UCGoYbwzkUBtGlVFUE04rUbNc8qymVW4YEzwXkhsulARJclUJITI-RQ9nbRf8-wCxLw82GnBOt-CHWHKaivFORtSI0jNqgo8xQFN2wR50OJaUlKfry3_X8y-BwHX8</recordid><startdate>202412</startdate><enddate>202412</enddate><creator>Sigida, E. 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P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758-50ab8e41c23fce9edc218c81a031b4d29736d1b9c22439473c3487e7098b44463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>acid hydrolysis</topic><topic>Azospirillum doebereinerae</topic><topic>biosynthesis</topic><topic>chromatography</topic><topic>fractionation</topic><topic>genes</topic><topic>genetic relationships</topic><topic>microbiology</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>O-antigens</topic><topic>phenol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sigida, E. N.</creatorcontrib><creatorcontrib>Grinev, V. S.</creatorcontrib><creatorcontrib>Kokoulin, M. S.</creatorcontrib><creatorcontrib>Konnova, S. A.</creatorcontrib><creatorcontrib>Fedonenko, Y. 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The O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide followed by chromatographic fractionation. Monosaccharide analysis, including determination of absolute configurations and 1D and 2D ¹H and ¹³C NMR spectroscopy, revealed the following structure of the O-specific polysaccharide repeating unit of A. agricola CC-HIH038ᵀ: →3)-α-L-Rhap2Ac-(1 → 3)-α-L-Rhap-(1 → 3)-α-L-Rhap-(1 → 3)-β-D-GlcpNAc6Ac-(1→. This unit is structurally related to that of A. doebereinerae GSF71ᵀ. Based on the analysis of full-genome sequencing data for strains A. agricola CC-HIH038ᵀ and A. doebereinerae GSF71ᵀ, the loci for O-specific polysaccharide biosynthesis have been identified. They were characterized by similar organization and a high level of gene homology, confirming the common structure of the O-antigens of these strains.</abstract><doi>10.1134/S0026261724606948</doi><tpages>9</tpages></addata></record> |
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| source | Springer Nature - Complete Springer Journals |
| subjects | acid hydrolysis Azospirillum doebereinerae biosynthesis chromatography fractionation genes genetic relationships microbiology nuclear magnetic resonance spectroscopy O-antigens phenol |
| title | Structural and Genetic Relationship of the O-Antigens of the Type Strains Azospirillum agricola CC-HIH038 and Azospirillum doebereinerae GSF71 |
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