Jannaschia ovalis sp. nov., a marine bacterium isolated from a tidal flat sediment in the Republic of Korea

A Gram-stain-negative, non-motile, aerobic, pale-yellow coloured and oval-shaped bacterium designated GRR-S6-38 T was isolated from a tidal flat sediment that collected from Garorim Bay of the Yellow Sea, Republic of Korea. Strain GRR-S6-38 T grew at 15–40 °C (optimum, 30 °C) at pH 6–9 (optimum, pH...

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Veröffentlicht in:Antonie van Leeuwenhoek 2025-01, Vol.118 (1), p.36-36, Article 36
Hauptverfasser: Kwon, Dae Young, Lee, Sang Hyun, Khim, Jong Seong, Xu, Xiaoyue, Kim, Bogun, Choi, In-Geol, Yoo, Yeonjae, Kim, Jae-Jin
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container_title Antonie van Leeuwenhoek
container_volume 118
creator Kwon, Dae Young
Lee, Sang Hyun
Khim, Jong Seong
Xu, Xiaoyue
Kim, Bogun
Choi, In-Geol
Yoo, Yeonjae
Kim, Jae-Jin
description A Gram-stain-negative, non-motile, aerobic, pale-yellow coloured and oval-shaped bacterium designated GRR-S6-38 T was isolated from a tidal flat sediment that collected from Garorim Bay of the Yellow Sea, Republic of Korea. Strain GRR-S6-38 T grew at 15–40 °C (optimum, 30 °C) at pH 6–9 (optimum, pH 7) and at 2–6% (w/v) NaCl (optimum, 2% NaCl). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GRR-S6-38 T was closely related to those of J. seosinensis CL-SP26 T (98.1%), J. helgolandensis DSM 14858 T (97.4%), J. rubra CECT 5088 T (97.4%), J. faecimaris DSM 100420 T (97.4%), J. marina SHC163 T (97.3%), J. donghaensis CECT 7802 T (97.2%) and J. pohangensis DSM 19073 T (97.1%). The average nucleotide identity and digital DNA-DNA hybridization value between GRR-S6-38 T and related type strains were 71.47–78.59% and 17.80–21.40%. Strain GRR-S6-38 T was characterized as having Q-10 as the predominant respiratory quinone and the major principle fatty acids (> 10%) were SF 8 (C 18:1 ω 7 c /C 18:1 ω 6 c , 39.3%), C 19:0 ω 8 c cyclo (13.2%) and C 18:1 ω 7 c 11-methyl (10.6%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified lipid, two unidentified aminolipids and two unidentified phospholipids. The assembled genome of strain GRR-S6-38 T has a total length of 3.32 Mbp, and a G + C content is 69.98%. Based on the polyphasic taxonomic evidence presented in this study, GRR-S6-38 T is regarded to represent a novel species within the genus Jannaschia , for which name Jannaschia ovalis sp. nov. is proposed. The type strain is GRR-S6-38 T (= KCTC 82518 = KACC 22240 = JCM 36187).
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Strain GRR-S6-38 T grew at 15–40 °C (optimum, 30 °C) at pH 6–9 (optimum, pH 7) and at 2–6% (w/v) NaCl (optimum, 2% NaCl). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GRR-S6-38 T was closely related to those of J. seosinensis CL-SP26 T (98.1%), J. helgolandensis DSM 14858 T (97.4%), J. rubra CECT 5088 T (97.4%), J. faecimaris DSM 100420 T (97.4%), J. marina SHC163 T (97.3%), J. donghaensis CECT 7802 T (97.2%) and J. pohangensis DSM 19073 T (97.1%). The average nucleotide identity and digital DNA-DNA hybridization value between GRR-S6-38 T and related type strains were 71.47–78.59% and 17.80–21.40%. Strain GRR-S6-38 T was characterized as having Q-10 as the predominant respiratory quinone and the major principle fatty acids (&gt; 10%) were SF 8 (C 18:1 ω 7 c /C 18:1 ω 6 c , 39.3%), C 19:0 ω 8 c cyclo (13.2%) and C 18:1 ω 7 c 11-methyl (10.6%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified lipid, two unidentified aminolipids and two unidentified phospholipids. The assembled genome of strain GRR-S6-38 T has a total length of 3.32 Mbp, and a G + C content is 69.98%. Based on the polyphasic taxonomic evidence presented in this study, GRR-S6-38 T is regarded to represent a novel species within the genus Jannaschia , for which name Jannaschia ovalis sp. nov. is proposed. 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Jan 2025</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c289t-940a0acd833c379b049d753d1b8afe3b5fd719bd549d7e530cf432b659e71a0f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10482-024-02044-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10482-024-02044-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39612060$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kwon, Dae Young</creatorcontrib><creatorcontrib>Lee, Sang Hyun</creatorcontrib><creatorcontrib>Khim, Jong Seong</creatorcontrib><creatorcontrib>Xu, Xiaoyue</creatorcontrib><creatorcontrib>Kim, Bogun</creatorcontrib><creatorcontrib>Choi, In-Geol</creatorcontrib><creatorcontrib>Yoo, Yeonjae</creatorcontrib><creatorcontrib>Kim, Jae-Jin</creatorcontrib><title>Jannaschia ovalis sp. nov., a marine bacterium isolated from a tidal flat sediment in the Republic of Korea</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie van Leeuwenhoek</addtitle><addtitle>Antonie Van Leeuwenhoek</addtitle><description>A Gram-stain-negative, non-motile, aerobic, pale-yellow coloured and oval-shaped bacterium designated GRR-S6-38 T was isolated from a tidal flat sediment that collected from Garorim Bay of the Yellow Sea, Republic of Korea. Strain GRR-S6-38 T grew at 15–40 °C (optimum, 30 °C) at pH 6–9 (optimum, pH 7) and at 2–6% (w/v) NaCl (optimum, 2% NaCl). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GRR-S6-38 T was closely related to those of J. seosinensis CL-SP26 T (98.1%), J. helgolandensis DSM 14858 T (97.4%), J. rubra CECT 5088 T (97.4%), J. faecimaris DSM 100420 T (97.4%), J. marina SHC163 T (97.3%), J. donghaensis CECT 7802 T (97.2%) and J. pohangensis DSM 19073 T (97.1%). The average nucleotide identity and digital DNA-DNA hybridization value between GRR-S6-38 T and related type strains were 71.47–78.59% and 17.80–21.40%. Strain GRR-S6-38 T was characterized as having Q-10 as the predominant respiratory quinone and the major principle fatty acids (&gt; 10%) were SF 8 (C 18:1 ω 7 c /C 18:1 ω 6 c , 39.3%), C 19:0 ω 8 c cyclo (13.2%) and C 18:1 ω 7 c 11-methyl (10.6%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified lipid, two unidentified aminolipids and two unidentified phospholipids. The assembled genome of strain GRR-S6-38 T has a total length of 3.32 Mbp, and a G + C content is 69.98%. Based on the polyphasic taxonomic evidence presented in this study, GRR-S6-38 T is regarded to represent a novel species within the genus Jannaschia , for which name Jannaschia ovalis sp. nov. is proposed. The type strain is GRR-S6-38 T (= KCTC 82518 = KACC 22240 = JCM 36187).</description><subject>aquatic bacteria</subject><subject>Bacteria</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cardiolipin</subject><subject>color</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Environmental science</subject><subject>Fatty Acids - analysis</subject><subject>Fatty Acids - chemistry</subject><subject>genome</subject><subject>Genomes</subject><subject>genus</subject><subject>Geologic Sediments - microbiology</subject><subject>Hybridization</subject><subject>Lecithin</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>New species</subject><subject>Nucleic Acid Hybridization</subject><subject>nucleotide sequences</subject><subject>Nucleotides</subject><subject>Original Paper</subject><subject>Phosphatidylcholine</subject><subject>phosphatidylcholines</subject><subject>Phosphatidylethanolamine</subject><subject>phosphatidylethanolamines</subject><subject>Phosphatidylglycerol</subject><subject>Phospholipids</subject><subject>Phospholipids - analysis</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Sciences</subject><subject>Quinones</subject><subject>Republic of Korea</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Seawater - microbiology</subject><subject>Sediments</subject><subject>Sequence Analysis, DNA</subject><subject>Sodium chloride</subject><subject>Soil Science &amp; Conservation</subject><subject>South Korea</subject><subject>species</subject><subject>Strain analysis</subject><subject>Taxonomy</subject><subject>Tidal flats</subject><subject>Yellow Sea</subject><issn>0003-6072</issn><issn>1572-9699</issn><issn>1572-9699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1rFTEUhoMo9lr9Ay4k4MaFcz35mMlkKcXWj4Igug6ZyYlNnUmuyUxp_725vVWhC3ERQk6e84ach5DnDLYMQL0pDGTPG-CyLpCyuX5ANqxVvNGd1g_JBgBE04HiR-RJKZf1qLtePSZHQneMQwcb8uOjjdGW8SJYmq7sFAotuy2N6Wr7mlo62xwi0sGOC-awzjSUNNkFHfU5zRVYgrMT9bVGC7owY1xoiHS5QPoFd-swhZEmTz-ljPYpeeTtVPDZ3X5Mvp2--3ryvjn_fPbh5O15M_JeL42WYMGOrhdiFEoPILVTrXBs6K1HMbTeKaYH1-7r2AoYvRR86FqNilnw4pi8OuTucvq5YlnMHMqI02QjprUYwVrJpVag_gMVEuow5R59eQ-9TGuO9SO3FNOsZbxS_ECNOZWS0ZtdDnWKN4aB2VszB2umWjO31sx1bXpxF70OM7o_Lb81VUAcgFKv4nfMf9_-R-wvO8KhaA</recordid><startdate>20250101</startdate><enddate>20250101</enddate><creator>Kwon, Dae Young</creator><creator>Lee, Sang Hyun</creator><creator>Khim, Jong Seong</creator><creator>Xu, Xiaoyue</creator><creator>Kim, Bogun</creator><creator>Choi, In-Geol</creator><creator>Yoo, Yeonjae</creator><creator>Kim, Jae-Jin</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20250101</creationdate><title>Jannaschia ovalis sp. nov., a marine bacterium isolated from a tidal flat sediment in the Republic of Korea</title><author>Kwon, Dae Young ; Lee, Sang Hyun ; Khim, Jong Seong ; Xu, Xiaoyue ; Kim, Bogun ; Choi, In-Geol ; Yoo, Yeonjae ; Kim, Jae-Jin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c289t-940a0acd833c379b049d753d1b8afe3b5fd719bd549d7e530cf432b659e71a0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>aquatic bacteria</topic><topic>Bacteria</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cardiolipin</topic><topic>color</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>Environmental science</topic><topic>Fatty Acids - analysis</topic><topic>Fatty Acids - chemistry</topic><topic>genome</topic><topic>Genomes</topic><topic>genus</topic><topic>Geologic Sediments - microbiology</topic><topic>Hybridization</topic><topic>Lecithin</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>New species</topic><topic>Nucleic Acid Hybridization</topic><topic>nucleotide sequences</topic><topic>Nucleotides</topic><topic>Original Paper</topic><topic>Phosphatidylcholine</topic><topic>phosphatidylcholines</topic><topic>Phosphatidylethanolamine</topic><topic>phosphatidylethanolamines</topic><topic>Phosphatidylglycerol</topic><topic>Phospholipids</topic><topic>Phospholipids - analysis</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Plant Sciences</topic><topic>Quinones</topic><topic>Republic of Korea</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Seawater - microbiology</topic><topic>Sediments</topic><topic>Sequence Analysis, DNA</topic><topic>Sodium chloride</topic><topic>Soil Science &amp; Conservation</topic><topic>South Korea</topic><topic>species</topic><topic>Strain analysis</topic><topic>Taxonomy</topic><topic>Tidal flats</topic><topic>Yellow Sea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kwon, Dae Young</creatorcontrib><creatorcontrib>Lee, Sang Hyun</creatorcontrib><creatorcontrib>Khim, Jong Seong</creatorcontrib><creatorcontrib>Xu, Xiaoyue</creatorcontrib><creatorcontrib>Kim, Bogun</creatorcontrib><creatorcontrib>Choi, In-Geol</creatorcontrib><creatorcontrib>Yoo, Yeonjae</creatorcontrib><creatorcontrib>Kim, Jae-Jin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; 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Strain GRR-S6-38 T grew at 15–40 °C (optimum, 30 °C) at pH 6–9 (optimum, pH 7) and at 2–6% (w/v) NaCl (optimum, 2% NaCl). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GRR-S6-38 T was closely related to those of J. seosinensis CL-SP26 T (98.1%), J. helgolandensis DSM 14858 T (97.4%), J. rubra CECT 5088 T (97.4%), J. faecimaris DSM 100420 T (97.4%), J. marina SHC163 T (97.3%), J. donghaensis CECT 7802 T (97.2%) and J. pohangensis DSM 19073 T (97.1%). The average nucleotide identity and digital DNA-DNA hybridization value between GRR-S6-38 T and related type strains were 71.47–78.59% and 17.80–21.40%. Strain GRR-S6-38 T was characterized as having Q-10 as the predominant respiratory quinone and the major principle fatty acids (&gt; 10%) were SF 8 (C 18:1 ω 7 c /C 18:1 ω 6 c , 39.3%), C 19:0 ω 8 c cyclo (13.2%) and C 18:1 ω 7 c 11-methyl (10.6%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified lipid, two unidentified aminolipids and two unidentified phospholipids. The assembled genome of strain GRR-S6-38 T has a total length of 3.32 Mbp, and a G + C content is 69.98%. Based on the polyphasic taxonomic evidence presented in this study, GRR-S6-38 T is regarded to represent a novel species within the genus Jannaschia , for which name Jannaschia ovalis sp. nov. is proposed. The type strain is GRR-S6-38 T (= KCTC 82518 = KACC 22240 = JCM 36187).</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>39612060</pmid><doi>10.1007/s10482-024-02044-x</doi><tpages>1</tpages></addata></record>
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subjects aquatic bacteria
Bacteria
Bacterial Typing Techniques
Base Composition
Biomedical and Life Sciences
Biotechnology
Cardiolipin
color
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
Environmental science
Fatty Acids - analysis
Fatty Acids - chemistry
genome
Genomes
genus
Geologic Sediments - microbiology
Hybridization
Lecithin
Life Sciences
Lipids
Medical Microbiology
Microbiology
New species
Nucleic Acid Hybridization
nucleotide sequences
Nucleotides
Original Paper
Phosphatidylcholine
phosphatidylcholines
Phosphatidylethanolamine
phosphatidylethanolamines
Phosphatidylglycerol
Phospholipids
Phospholipids - analysis
Phylogenetics
Phylogeny
Plant Sciences
Quinones
Republic of Korea
RNA, Ribosomal, 16S - genetics
rRNA 16S
Seawater - microbiology
Sediments
Sequence Analysis, DNA
Sodium chloride
Soil Science & Conservation
South Korea
species
Strain analysis
Taxonomy
Tidal flats
Yellow Sea
title Jannaschia ovalis sp. nov., a marine bacterium isolated from a tidal flat sediment in the Republic of Korea
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