Effects of phosphorylation on CsTT12 transport function: A comparative phosphoproteomic analysis of flavonoid biosynthesis in tea plants (Camellia sinensis)
SUMMARY Monomeric flavan‐3‐ols and their oligomeric forms, proanthocyanidins (PAs), are closely related to the bitterness of tea beverages. Monomeric flavan‐3‐ols are characteristic flavor compounds in tea. Increasing the content of PAs and anthocyanins enhances the resistance of tea plants to patho...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2024-12, Vol.120 (6), p.2420-2436 |
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| creator | Wang, Na‐Na Xiu, Ke‐Yan Deng, Min Liu, Qi‐Yun Jin, Di‐Di Zhao, Qiao‐Mei Su, Huang‐Qiang Qiu, Ting‐ting Wang, Hai‐Yan Liu, Ya‐Jun Jiang, Xiao‐Lan Xia, Tao Gao, Li‐Ping |
| description | SUMMARY
Monomeric flavan‐3‐ols and their oligomeric forms, proanthocyanidins (PAs), are closely related to the bitterness of tea beverages. Monomeric flavan‐3‐ols are characteristic flavor compounds in tea. Increasing the content of PAs and anthocyanins enhances the resistance of tea plants to pathogen invasion but decreases the quality of tea beverages. MATE family transporters play a critical role in transferring monomeric flavan‐3‐ols and anthocyanins into vacuoles for storage or subsequent condensation into PAs. Their activities modulate the ratio of monomeric flavan‐3‐ols to PAs and increase anthocyanin content in tea plants. In this study, it was observed that the gene expression and protein phosphorylation level of the MATE transporter CsTT12, a vacuole‐localized flavonoid transporter, were notably upregulated following exogenous sucrose treatment, promoting PA synthesis in tea plants. Further analysis revealed that overexpression of CsTT12 and CsTT12S17D significantly increased the content of anthocyanins and PAs in plants, whereas CsTT12S17A did not. In CsTT12 knockdown plants, PA's accumulation decreased significantly, while monomeric catechin content increased. Moreover, phosphorylation modification enhanced the vacuolar membrane localization of CsTT12, whereas dephosphorylation weakened its vacuolar membrane localization. This study uncovers the crucial role of phosphorylation in flavonoid biosynthesis and provides insights into balancing quality improvements and resistance enhancement.
Significance Statement
CsTT12 promotes flavonoid biosynthesis by functioning as a MATE transporter, facilitating the transfer of monomeric flavan‐3‐ols into the vacuole for proanthocyanidin (PA) accumulation. Phosphorylation of CsTT12 at Ser‐17 is crucial for its localization to the tonoplast, which subsequently influences its transport function in tea plants. |
| doi_str_mv | 10.1111/tpj.17120 |
| format | Article |
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Monomeric flavan‐3‐ols and their oligomeric forms, proanthocyanidins (PAs), are closely related to the bitterness of tea beverages. Monomeric flavan‐3‐ols are characteristic flavor compounds in tea. Increasing the content of PAs and anthocyanins enhances the resistance of tea plants to pathogen invasion but decreases the quality of tea beverages. MATE family transporters play a critical role in transferring monomeric flavan‐3‐ols and anthocyanins into vacuoles for storage or subsequent condensation into PAs. Their activities modulate the ratio of monomeric flavan‐3‐ols to PAs and increase anthocyanin content in tea plants. In this study, it was observed that the gene expression and protein phosphorylation level of the MATE transporter CsTT12, a vacuole‐localized flavonoid transporter, were notably upregulated following exogenous sucrose treatment, promoting PA synthesis in tea plants. Further analysis revealed that overexpression of CsTT12 and CsTT12S17D significantly increased the content of anthocyanins and PAs in plants, whereas CsTT12S17A did not. In CsTT12 knockdown plants, PA's accumulation decreased significantly, while monomeric catechin content increased. Moreover, phosphorylation modification enhanced the vacuolar membrane localization of CsTT12, whereas dephosphorylation weakened its vacuolar membrane localization. This study uncovers the crucial role of phosphorylation in flavonoid biosynthesis and provides insights into balancing quality improvements and resistance enhancement.
Significance Statement
CsTT12 promotes flavonoid biosynthesis by functioning as a MATE transporter, facilitating the transfer of monomeric flavan‐3‐ols into the vacuole for proanthocyanidin (PA) accumulation. Phosphorylation of CsTT12 at Ser‐17 is crucial for its localization to the tonoplast, which subsequently influences its transport function in tea plants.</description><identifier>ISSN: 0960-7412</identifier><identifier>ISSN: 1365-313X</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/tpj.17120</identifier><identifier>PMID: 39488740</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Anthocyanins ; Anthocyanins - metabolism ; Aroma compounds ; Beverages ; Biological Transport ; Biosynthesis ; Bitterness ; Camellia sinensis ; Camellia sinensis - genetics ; Camellia sinensis - metabolism ; Catechin ; Catechin - metabolism ; Dephosphorylation ; family ; flavan‐3‐ols, proanthocyanidins (PAs) ; flavonoid transport ; Flavonoids ; Flavonoids - metabolism ; Flavor compounds ; Gene expression ; Gene Expression Regulation, Plant ; Localization ; Membranes ; multidrug and toxic compound extrusion (MATE) transporters ; pathogens ; phosphoproteomics ; Phosphorylation ; Plant Proteins - genetics ; Plant Proteins - metabolism ; proanthocyanidins ; Proanthocyanidins - metabolism ; protein phosphorylation ; Protein transport ; Proteomics ; Sucrose ; Tea ; tea plants (Camellia sinensis) ; Vacuoles ; Vacuoles - metabolism</subject><ispartof>The Plant journal : for cell and molecular biology, 2024-12, Vol.120 (6), p.2420-2436</ispartof><rights>2024 Society for Experimental Biology and John Wiley & Sons Ltd.</rights><rights>Copyright © 2024 Society for Experimental Biology and John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2760-842e36da717c0ad6b583adf7aafed49b6c2670f7b593c1c7fcd000156f55fa623</cites><orcidid>0000-0002-3940-688X ; 0000-0002-5109-8285</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftpj.17120$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftpj.17120$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39488740$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Na‐Na</creatorcontrib><creatorcontrib>Xiu, Ke‐Yan</creatorcontrib><creatorcontrib>Deng, Min</creatorcontrib><creatorcontrib>Liu, Qi‐Yun</creatorcontrib><creatorcontrib>Jin, Di‐Di</creatorcontrib><creatorcontrib>Zhao, Qiao‐Mei</creatorcontrib><creatorcontrib>Su, Huang‐Qiang</creatorcontrib><creatorcontrib>Qiu, Ting‐ting</creatorcontrib><creatorcontrib>Wang, Hai‐Yan</creatorcontrib><creatorcontrib>Liu, Ya‐Jun</creatorcontrib><creatorcontrib>Jiang, Xiao‐Lan</creatorcontrib><creatorcontrib>Xia, Tao</creatorcontrib><creatorcontrib>Gao, Li‐Ping</creatorcontrib><title>Effects of phosphorylation on CsTT12 transport function: A comparative phosphoproteomic analysis of flavonoid biosynthesis in tea plants (Camellia sinensis)</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>SUMMARY
Monomeric flavan‐3‐ols and their oligomeric forms, proanthocyanidins (PAs), are closely related to the bitterness of tea beverages. Monomeric flavan‐3‐ols are characteristic flavor compounds in tea. Increasing the content of PAs and anthocyanins enhances the resistance of tea plants to pathogen invasion but decreases the quality of tea beverages. MATE family transporters play a critical role in transferring monomeric flavan‐3‐ols and anthocyanins into vacuoles for storage or subsequent condensation into PAs. Their activities modulate the ratio of monomeric flavan‐3‐ols to PAs and increase anthocyanin content in tea plants. In this study, it was observed that the gene expression and protein phosphorylation level of the MATE transporter CsTT12, a vacuole‐localized flavonoid transporter, were notably upregulated following exogenous sucrose treatment, promoting PA synthesis in tea plants. Further analysis revealed that overexpression of CsTT12 and CsTT12S17D significantly increased the content of anthocyanins and PAs in plants, whereas CsTT12S17A did not. In CsTT12 knockdown plants, PA's accumulation decreased significantly, while monomeric catechin content increased. Moreover, phosphorylation modification enhanced the vacuolar membrane localization of CsTT12, whereas dephosphorylation weakened its vacuolar membrane localization. This study uncovers the crucial role of phosphorylation in flavonoid biosynthesis and provides insights into balancing quality improvements and resistance enhancement.
Significance Statement
CsTT12 promotes flavonoid biosynthesis by functioning as a MATE transporter, facilitating the transfer of monomeric flavan‐3‐ols into the vacuole for proanthocyanidin (PA) accumulation. Phosphorylation of CsTT12 at Ser‐17 is crucial for its localization to the tonoplast, which subsequently influences its transport function in tea plants.</description><subject>Anthocyanins</subject><subject>Anthocyanins - metabolism</subject><subject>Aroma compounds</subject><subject>Beverages</subject><subject>Biological Transport</subject><subject>Biosynthesis</subject><subject>Bitterness</subject><subject>Camellia sinensis</subject><subject>Camellia sinensis - genetics</subject><subject>Camellia sinensis - metabolism</subject><subject>Catechin</subject><subject>Catechin - metabolism</subject><subject>Dephosphorylation</subject><subject>family</subject><subject>flavan‐3‐ols, proanthocyanidins (PAs)</subject><subject>flavonoid transport</subject><subject>Flavonoids</subject><subject>Flavonoids - metabolism</subject><subject>Flavor compounds</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Localization</subject><subject>Membranes</subject><subject>multidrug and toxic compound extrusion (MATE) transporters</subject><subject>pathogens</subject><subject>phosphoproteomics</subject><subject>Phosphorylation</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>proanthocyanidins</subject><subject>Proanthocyanidins - metabolism</subject><subject>protein phosphorylation</subject><subject>Protein transport</subject><subject>Proteomics</subject><subject>Sucrose</subject><subject>Tea</subject><subject>tea plants (Camellia sinensis)</subject><subject>Vacuoles</subject><subject>Vacuoles - metabolism</subject><issn>0960-7412</issn><issn>1365-313X</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9qFTEUxoMo9lpd-AIScNMubpv_meuuXGq1FHRxBXfDmUxCc5lJxmSmMg8j9Fn6ZM3tbbsQBMMJWZzf-fhyPoTeU3JCyzkdh-0J1ZSRF2hBuZJLTvnPl2hBVoostaDsAL3JeUsI1VyJ1-iAr0RVaUEW6M-5c9aMGUeHh-uYy01zB6OPAZda582GMjwmCHmIacRuCmbX_ITP7m5N7AdIBb6xT8NDiqONvTcYAnRz9g_KroObGKJvceNjnsN4bXcdH_BoAQ8dhOLgaA297ToPOPtgQwGO36JXDrps3z2-h-jH5_PN-svy6tvF1_XZ1dIwXb5YCWa5akFTbQi0qpEVh9ZpAGdbsWqUYUoTpxu54oYa7UxLyjKkclI6UIwfoqO9brH_a7J5rHufTTEDwcYp15xKwbhmvPoPlHEpiahkQT_-hW7jlMpadpTQRDMleKGO95RJMedkXT0k30Oaa0rqXbx1ibd-iLewHx4Vp6a37TP5lGcBTvfAb9_Z-d9K9eb75V7yHmiqsmA</recordid><startdate>202412</startdate><enddate>202412</enddate><creator>Wang, Na‐Na</creator><creator>Xiu, Ke‐Yan</creator><creator>Deng, Min</creator><creator>Liu, Qi‐Yun</creator><creator>Jin, Di‐Di</creator><creator>Zhao, Qiao‐Mei</creator><creator>Su, Huang‐Qiang</creator><creator>Qiu, Ting‐ting</creator><creator>Wang, Hai‐Yan</creator><creator>Liu, Ya‐Jun</creator><creator>Jiang, Xiao‐Lan</creator><creator>Xia, Tao</creator><creator>Gao, Li‐Ping</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-3940-688X</orcidid><orcidid>https://orcid.org/0000-0002-5109-8285</orcidid></search><sort><creationdate>202412</creationdate><title>Effects of phosphorylation on CsTT12 transport function: A comparative phosphoproteomic analysis of flavonoid biosynthesis in tea plants (Camellia sinensis)</title><author>Wang, Na‐Na ; Xiu, Ke‐Yan ; Deng, Min ; Liu, Qi‐Yun ; Jin, Di‐Di ; Zhao, Qiao‐Mei ; Su, Huang‐Qiang ; Qiu, Ting‐ting ; Wang, Hai‐Yan ; Liu, Ya‐Jun ; Jiang, Xiao‐Lan ; Xia, Tao ; Gao, Li‐Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2760-842e36da717c0ad6b583adf7aafed49b6c2670f7b593c1c7fcd000156f55fa623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Anthocyanins</topic><topic>Anthocyanins - metabolism</topic><topic>Aroma compounds</topic><topic>Beverages</topic><topic>Biological Transport</topic><topic>Biosynthesis</topic><topic>Bitterness</topic><topic>Camellia sinensis</topic><topic>Camellia sinensis - genetics</topic><topic>Camellia sinensis - metabolism</topic><topic>Catechin</topic><topic>Catechin - metabolism</topic><topic>Dephosphorylation</topic><topic>family</topic><topic>flavan‐3‐ols, proanthocyanidins (PAs)</topic><topic>flavonoid transport</topic><topic>Flavonoids</topic><topic>Flavonoids - metabolism</topic><topic>Flavor compounds</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Localization</topic><topic>Membranes</topic><topic>multidrug and toxic compound extrusion (MATE) transporters</topic><topic>pathogens</topic><topic>phosphoproteomics</topic><topic>Phosphorylation</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>proanthocyanidins</topic><topic>Proanthocyanidins - metabolism</topic><topic>protein phosphorylation</topic><topic>Protein transport</topic><topic>Proteomics</topic><topic>Sucrose</topic><topic>Tea</topic><topic>tea plants (Camellia sinensis)</topic><topic>Vacuoles</topic><topic>Vacuoles - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Na‐Na</creatorcontrib><creatorcontrib>Xiu, Ke‐Yan</creatorcontrib><creatorcontrib>Deng, Min</creatorcontrib><creatorcontrib>Liu, Qi‐Yun</creatorcontrib><creatorcontrib>Jin, Di‐Di</creatorcontrib><creatorcontrib>Zhao, Qiao‐Mei</creatorcontrib><creatorcontrib>Su, Huang‐Qiang</creatorcontrib><creatorcontrib>Qiu, Ting‐ting</creatorcontrib><creatorcontrib>Wang, Hai‐Yan</creatorcontrib><creatorcontrib>Liu, Ya‐Jun</creatorcontrib><creatorcontrib>Jiang, Xiao‐Lan</creatorcontrib><creatorcontrib>Xia, Tao</creatorcontrib><creatorcontrib>Gao, Li‐Ping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Na‐Na</au><au>Xiu, Ke‐Yan</au><au>Deng, Min</au><au>Liu, Qi‐Yun</au><au>Jin, Di‐Di</au><au>Zhao, Qiao‐Mei</au><au>Su, Huang‐Qiang</au><au>Qiu, Ting‐ting</au><au>Wang, Hai‐Yan</au><au>Liu, Ya‐Jun</au><au>Jiang, Xiao‐Lan</au><au>Xia, Tao</au><au>Gao, Li‐Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of phosphorylation on CsTT12 transport function: A comparative phosphoproteomic analysis of flavonoid biosynthesis in tea plants (Camellia sinensis)</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2024-12</date><risdate>2024</risdate><volume>120</volume><issue>6</issue><spage>2420</spage><epage>2436</epage><pages>2420-2436</pages><issn>0960-7412</issn><issn>1365-313X</issn><eissn>1365-313X</eissn><abstract>SUMMARY
Monomeric flavan‐3‐ols and their oligomeric forms, proanthocyanidins (PAs), are closely related to the bitterness of tea beverages. Monomeric flavan‐3‐ols are characteristic flavor compounds in tea. Increasing the content of PAs and anthocyanins enhances the resistance of tea plants to pathogen invasion but decreases the quality of tea beverages. MATE family transporters play a critical role in transferring monomeric flavan‐3‐ols and anthocyanins into vacuoles for storage or subsequent condensation into PAs. Their activities modulate the ratio of monomeric flavan‐3‐ols to PAs and increase anthocyanin content in tea plants. In this study, it was observed that the gene expression and protein phosphorylation level of the MATE transporter CsTT12, a vacuole‐localized flavonoid transporter, were notably upregulated following exogenous sucrose treatment, promoting PA synthesis in tea plants. Further analysis revealed that overexpression of CsTT12 and CsTT12S17D significantly increased the content of anthocyanins and PAs in plants, whereas CsTT12S17A did not. In CsTT12 knockdown plants, PA's accumulation decreased significantly, while monomeric catechin content increased. Moreover, phosphorylation modification enhanced the vacuolar membrane localization of CsTT12, whereas dephosphorylation weakened its vacuolar membrane localization. This study uncovers the crucial role of phosphorylation in flavonoid biosynthesis and provides insights into balancing quality improvements and resistance enhancement.
Significance Statement
CsTT12 promotes flavonoid biosynthesis by functioning as a MATE transporter, facilitating the transfer of monomeric flavan‐3‐ols into the vacuole for proanthocyanidin (PA) accumulation. Phosphorylation of CsTT12 at Ser‐17 is crucial for its localization to the tonoplast, which subsequently influences its transport function in tea plants.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>39488740</pmid><doi>10.1111/tpj.17120</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0002-3940-688X</orcidid><orcidid>https://orcid.org/0000-0002-5109-8285</orcidid></addata></record> |
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| ispartof | The Plant journal : for cell and molecular biology, 2024-12, Vol.120 (6), p.2420-2436 |
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| subjects | Anthocyanins Anthocyanins - metabolism Aroma compounds Beverages Biological Transport Biosynthesis Bitterness Camellia sinensis Camellia sinensis - genetics Camellia sinensis - metabolism Catechin Catechin - metabolism Dephosphorylation family flavan‐3‐ols, proanthocyanidins (PAs) flavonoid transport Flavonoids Flavonoids - metabolism Flavor compounds Gene expression Gene Expression Regulation, Plant Localization Membranes multidrug and toxic compound extrusion (MATE) transporters pathogens phosphoproteomics Phosphorylation Plant Proteins - genetics Plant Proteins - metabolism proanthocyanidins Proanthocyanidins - metabolism protein phosphorylation Protein transport Proteomics Sucrose Tea tea plants (Camellia sinensis) Vacuoles Vacuoles - metabolism |
| title | Effects of phosphorylation on CsTT12 transport function: A comparative phosphoproteomic analysis of flavonoid biosynthesis in tea plants (Camellia sinensis) |
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