From sequence to significance: A thorough investigation of the distinctive genome features uncovered in C. Werkmanii strain NIB003

•The strain NIB003 of C. werkmanii has been sequenced for the first time in Bangladesh.•The investigation of the pangenome uncovers distinct genetic characteristics in NIB003 strain.•NIB003 strain reveals the presence of specific virulence factors and antibiotic resistance genes.•The proteins involved in unique characteristics exhibit structural stability, as proven by molecular dynamics. Citrobacter werkmanii (C. werkmanii), an opportunistic urinary bacterium that causes diarrhea, is poorly understood. Our research focuses on genetic features that are crucial to disease development, such as pathogenic interactions, antibiotic resistance, virulence genes and genetic variation. Following its morphological, biochemical, and molecular identification, the whole genome of C. werkmanii strain NIB003 was sequenced in Bangladesh for the first time. Despite having around 80% whole genome conservation, the research shows that the Bangladeshi strain forms a separate phylogenetic cluster. This emphasises the genetic variability within C. werkmanii, resulting in particular modifications at the strain level and changes in its ability to cause disease. The results of the genetic diversity analysis indicate that the Bangladeshi sequenced genome is more diverse than the other strains due to the existence of unique features, such as the presence of t-RNA binding domain and N-6 adenine-specific DNA methylases.