Establishment and identification of the gill cell line from the blunt snout bream (Megalobrama amblycephala) and its application in studying gill remodeling under hypoxia
To probe the mechanisms of gill remodeling in blunt snout bream under hypoxic conditions, we selected gill tissue for primary cell culture to establish and characterize the first blunt snout bream gill cell line, named MAG. The gill cells were efficiently passaged in M199 medium supplemented with 8%...
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| creator | Xu, Wenya Feng, Yahui Chen, Songlin Wang, Huihu Wen, Jian Zheng, Guodong Wang, Ganxiang Zou, Shuming |
| description | To probe the mechanisms of gill remodeling in blunt snout bream under hypoxic conditions, we selected gill tissue for primary cell culture to establish and characterize the first blunt snout bream gill cell line, named MAG. The gill cells were efficiently passaged in M199 medium supplemented with 8% antibiotics and 15% fetal bovine serum at 28 °C, exhibiting primarily an epithelial-fibroblast mixed type. Additionally, the MAG cells (17th generation) were subjected to four experimental conditions—normoxia, hypoxia 12 h, hypoxia 24 h, and reoxygenation 24 h (R24h)—to evaluate the effects of hypoxia and reoxygenation on MAG cells during gill remodeling. We found that the MAG cell morphology underwent shrinkage and mitochondrial potential gradually lost, even leading to gradual apoptosis with increasing hypoxia duration and increased reactive oxygen species (ROS) activity. Upon reoxygenation, MAG cells gradually regain cellular homeostasis, accompanied by a decrease in ROS activity. Analysis of superoxide dismutase (SOD), glutathione (GSH), lactate dehydrogenase (LDH), catalase (CAT), anti-superoxide anion, and other enzyme activities revealed enhanced antioxidant enzyme activity in MAG cells during hypoxia, aiding in adapting to hypoxic stress and preserving cell morphology. After reoxygenation, the cells gradually returned to normoxic levels. Our findings underscore the MAG cells can be used to study hypoxic cell apoptosis during gill remodeling. Therefore, the MAG cell line will serve as a vital in vitro model for exploring gill remodeling in blunt snout bream under hypoxia. |
| doi_str_mv | 10.1007/s10695-024-01393-8 |
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The gill cells were efficiently passaged in M199 medium supplemented with 8% antibiotics and 15% fetal bovine serum at 28 °C, exhibiting primarily an epithelial-fibroblast mixed type. Additionally, the MAG cells (17th generation) were subjected to four experimental conditions—normoxia, hypoxia 12 h, hypoxia 24 h, and reoxygenation 24 h (R24h)—to evaluate the effects of hypoxia and reoxygenation on MAG cells during gill remodeling. We found that the MAG cell morphology underwent shrinkage and mitochondrial potential gradually lost, even leading to gradual apoptosis with increasing hypoxia duration and increased reactive oxygen species (ROS) activity. Upon reoxygenation, MAG cells gradually regain cellular homeostasis, accompanied by a decrease in ROS activity. Analysis of superoxide dismutase (SOD), glutathione (GSH), lactate dehydrogenase (LDH), catalase (CAT), anti-superoxide anion, and other enzyme activities revealed enhanced antioxidant enzyme activity in MAG cells during hypoxia, aiding in adapting to hypoxic stress and preserving cell morphology. After reoxygenation, the cells gradually returned to normoxic levels. Our findings underscore the MAG cells can be used to study hypoxic cell apoptosis during gill remodeling. Therefore, the MAG cell line will serve as a vital in vitro model for exploring gill remodeling in blunt snout bream under hypoxia.</description><identifier>ISSN: 0920-1742</identifier><identifier>ISSN: 1573-5168</identifier><identifier>EISSN: 1573-5168</identifier><identifier>DOI: 10.1007/s10695-024-01393-8</identifier><identifier>PMID: 39222227</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Animal Physiology ; Animals ; Anions ; Antibiotics ; antioxidant enzymes ; Apoptosis ; Apoptosis - drug effects ; Biomedical and Life Sciences ; Bream ; Catalase ; Catalase - metabolism ; Cell culture ; Cell Hypoxia ; Cell Line ; Cell lines ; Cell morphology ; Cells ; Cyprinidae - physiology ; Enzymatic activity ; Enzyme activity ; Enzymes ; fetal bovine serum ; fish ; Freshwater & Marine Ecology ; Gills - cytology ; Gills - drug effects ; Gills - pathology ; Glutathione ; Histology ; Homeostasis ; Hypoxia ; L-Lactate dehydrogenase ; Lactate ; Lactate dehydrogenase ; Life Sciences ; Megalobrama amblycephala ; mitochondria ; Morphology ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; shrinkage ; Superoxide anions ; Superoxide dismutase ; Superoxide Dismutase - metabolism ; Tissue culture ; Zoology</subject><ispartof>Fish physiology and biochemistry, 2024-12, Vol.50 (6), p.2475-2488</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. 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The gill cells were efficiently passaged in M199 medium supplemented with 8% antibiotics and 15% fetal bovine serum at 28 °C, exhibiting primarily an epithelial-fibroblast mixed type. Additionally, the MAG cells (17th generation) were subjected to four experimental conditions—normoxia, hypoxia 12 h, hypoxia 24 h, and reoxygenation 24 h (R24h)—to evaluate the effects of hypoxia and reoxygenation on MAG cells during gill remodeling. We found that the MAG cell morphology underwent shrinkage and mitochondrial potential gradually lost, even leading to gradual apoptosis with increasing hypoxia duration and increased reactive oxygen species (ROS) activity. Upon reoxygenation, MAG cells gradually regain cellular homeostasis, accompanied by a decrease in ROS activity. Analysis of superoxide dismutase (SOD), glutathione (GSH), lactate dehydrogenase (LDH), catalase (CAT), anti-superoxide anion, and other enzyme activities revealed enhanced antioxidant enzyme activity in MAG cells during hypoxia, aiding in adapting to hypoxic stress and preserving cell morphology. After reoxygenation, the cells gradually returned to normoxic levels. Our findings underscore the MAG cells can be used to study hypoxic cell apoptosis during gill remodeling. Therefore, the MAG cell line will serve as a vital in vitro model for exploring gill remodeling in blunt snout bream under hypoxia.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animal Physiology</subject><subject>Animals</subject><subject>Anions</subject><subject>Antibiotics</subject><subject>antioxidant enzymes</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biomedical and Life Sciences</subject><subject>Bream</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Cell culture</subject><subject>Cell Hypoxia</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell morphology</subject><subject>Cells</subject><subject>Cyprinidae - physiology</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>fetal bovine serum</subject><subject>fish</subject><subject>Freshwater & Marine Ecology</subject><subject>Gills - 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Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Fish physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Wenya</au><au>Feng, Yahui</au><au>Chen, Songlin</au><au>Wang, Huihu</au><au>Wen, Jian</au><au>Zheng, Guodong</au><au>Wang, Ganxiang</au><au>Zou, Shuming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment and identification of the gill cell line from the blunt snout bream (Megalobrama amblycephala) and its application in studying gill remodeling under hypoxia</atitle><jtitle>Fish physiology and biochemistry</jtitle><stitle>Fish Physiol Biochem</stitle><addtitle>Fish Physiol Biochem</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>50</volume><issue>6</issue><spage>2475</spage><epage>2488</epage><pages>2475-2488</pages><issn>0920-1742</issn><issn>1573-5168</issn><eissn>1573-5168</eissn><abstract>To probe the mechanisms of gill remodeling in blunt snout bream under hypoxic conditions, we selected gill tissue for primary cell culture to establish and characterize the first blunt snout bream gill cell line, named MAG. The gill cells were efficiently passaged in M199 medium supplemented with 8% antibiotics and 15% fetal bovine serum at 28 °C, exhibiting primarily an epithelial-fibroblast mixed type. Additionally, the MAG cells (17th generation) were subjected to four experimental conditions—normoxia, hypoxia 12 h, hypoxia 24 h, and reoxygenation 24 h (R24h)—to evaluate the effects of hypoxia and reoxygenation on MAG cells during gill remodeling. We found that the MAG cell morphology underwent shrinkage and mitochondrial potential gradually lost, even leading to gradual apoptosis with increasing hypoxia duration and increased reactive oxygen species (ROS) activity. Upon reoxygenation, MAG cells gradually regain cellular homeostasis, accompanied by a decrease in ROS activity. Analysis of superoxide dismutase (SOD), glutathione (GSH), lactate dehydrogenase (LDH), catalase (CAT), anti-superoxide anion, and other enzyme activities revealed enhanced antioxidant enzyme activity in MAG cells during hypoxia, aiding in adapting to hypoxic stress and preserving cell morphology. After reoxygenation, the cells gradually returned to normoxic levels. Our findings underscore the MAG cells can be used to study hypoxic cell apoptosis during gill remodeling. Therefore, the MAG cell line will serve as a vital in vitro model for exploring gill remodeling in blunt snout bream under hypoxia.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>39222227</pmid><doi>10.1007/s10695-024-01393-8</doi><tpages>14</tpages></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Animal Physiology Animals Anions Antibiotics antioxidant enzymes Apoptosis Apoptosis - drug effects Biomedical and Life Sciences Bream Catalase Catalase - metabolism Cell culture Cell Hypoxia Cell Line Cell lines Cell morphology Cells Cyprinidae - physiology Enzymatic activity Enzyme activity Enzymes fetal bovine serum fish Freshwater & Marine Ecology Gills - cytology Gills - drug effects Gills - pathology Glutathione Histology Homeostasis Hypoxia L-Lactate dehydrogenase Lactate Lactate dehydrogenase Life Sciences Megalobrama amblycephala mitochondria Morphology Reactive oxygen species Reactive Oxygen Species - metabolism shrinkage Superoxide anions Superoxide dismutase Superoxide Dismutase - metabolism Tissue culture Zoology |
| title | Establishment and identification of the gill cell line from the blunt snout bream (Megalobrama amblycephala) and its application in studying gill remodeling under hypoxia |
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