Neutralization of Centruroides tecomanus scorpion venom by the use of two human recombinant antibody fragments

The first toxic component identified against mammals in the venom from Centruroides tecomanus scorpion from Colima, Mexico was Ct1a toxin, which was neutralized by human single chain variable fragment (scFv) RAS27. Venom characterization from these scorpions collected on the Pacific coast of Colima,...

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Veröffentlicht in:Molecular immunology 2023-12, Vol.164, p.79-87
Hauptverfasser: Valencia-Martínez, Hugo, Riaño-Umbarila, Lidia, Olamendi-Portugal, Timoteo, Romero-Moreno, José Alberto, Possani, Lourival D, Becerril, Baltazar
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container_end_page 87
container_issue
container_start_page 79
container_title Molecular immunology
container_volume 164
creator Valencia-Martínez, Hugo
Riaño-Umbarila, Lidia
Olamendi-Portugal, Timoteo
Romero-Moreno, José Alberto
Possani, Lourival D
Becerril, Baltazar
description The first toxic component identified against mammals in the venom from Centruroides tecomanus scorpion from Colima, Mexico was Ct1a toxin, which was neutralized by human single chain variable fragment (scFv) RAS27. Venom characterization from these scorpions collected on the Pacific coast of Colima, enabled the identification of a second component of medical importance named Ct71 toxin. Amino acid sequence of Ct71 shares a high identity with Chui5 toxin from C. huichol scorpion, which was neutralized by scFv HV. For this reason, the kinetic parameters of interaction between Ct71 toxin and scFv HV were determined by surface plasmon resonance. Results showed a significantly higher affinity for Ct71 as compared to Chui5. As expected, this toxin was neutralized by scFv HV. The injection of a mixture of scFvs HV and RAS27, resulted in the neutralization of C. tecomanus venom, corroborating that human recombinant antibody fragments can efficiently contribute to the neutralization of medically important toxins and their respective venoms from Mexican scorpions.
doi_str_mv 10.1016/j.molimm.2023.11.001
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subjects amino acid sequences
Animals
Centruroides
coasts
Humans
Mammals
Mexico
neutralization
recombinant antibodies
Recombinant Proteins - chemistry
Scorpion Venoms
Scorpions
Single-Chain Antibodies
surface plasmon resonance
toxicity
toxins
title Neutralization of Centruroides tecomanus scorpion venom by the use of two human recombinant antibody fragments
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