Neutralization of Centruroides tecomanus scorpion venom by the use of two human recombinant antibody fragments
The first toxic component identified against mammals in the venom from Centruroides tecomanus scorpion from Colima, Mexico was Ct1a toxin, which was neutralized by human single chain variable fragment (scFv) RAS27. Venom characterization from these scorpions collected on the Pacific coast of Colima,...
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Veröffentlicht in: | Molecular immunology 2023-12, Vol.164, p.79-87 |
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creator | Valencia-Martínez, Hugo Riaño-Umbarila, Lidia Olamendi-Portugal, Timoteo Romero-Moreno, José Alberto Possani, Lourival D Becerril, Baltazar |
description | The first toxic component identified against mammals in the venom from Centruroides tecomanus scorpion from Colima, Mexico was Ct1a toxin, which was neutralized by human single chain variable fragment (scFv) RAS27. Venom characterization from these scorpions collected on the Pacific coast of Colima, enabled the identification of a second component of medical importance named Ct71 toxin. Amino acid sequence of Ct71 shares a high identity with Chui5 toxin from C. huichol scorpion, which was neutralized by scFv HV. For this reason, the kinetic parameters of interaction between Ct71 toxin and scFv HV were determined by surface plasmon resonance. Results showed a significantly higher affinity for Ct71 as compared to Chui5. As expected, this toxin was neutralized by scFv HV. The injection of a mixture of scFvs HV and RAS27, resulted in the neutralization of C. tecomanus venom, corroborating that human recombinant antibody fragments can efficiently contribute to the neutralization of medically important toxins and their respective venoms from Mexican scorpions. |
doi_str_mv | 10.1016/j.molimm.2023.11.001 |
format | Article |
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Venom characterization from these scorpions collected on the Pacific coast of Colima, enabled the identification of a second component of medical importance named Ct71 toxin. Amino acid sequence of Ct71 shares a high identity with Chui5 toxin from C. huichol scorpion, which was neutralized by scFv HV. For this reason, the kinetic parameters of interaction between Ct71 toxin and scFv HV were determined by surface plasmon resonance. Results showed a significantly higher affinity for Ct71 as compared to Chui5. As expected, this toxin was neutralized by scFv HV. The injection of a mixture of scFvs HV and RAS27, resulted in the neutralization of C. tecomanus venom, corroborating that human recombinant antibody fragments can efficiently contribute to the neutralization of medically important toxins and their respective venoms from Mexican scorpions.</description><identifier>ISSN: 0161-5890</identifier><identifier>EISSN: 1872-9142</identifier><identifier>DOI: 10.1016/j.molimm.2023.11.001</identifier><identifier>PMID: 37980772</identifier><language>eng</language><publisher>England</publisher><subject>amino acid sequences ; Animals ; Centruroides ; coasts ; Humans ; Mammals ; Mexico ; neutralization ; recombinant antibodies ; Recombinant Proteins - chemistry ; Scorpion Venoms ; Scorpions ; Single-Chain Antibodies ; surface plasmon resonance ; toxicity ; toxins</subject><ispartof>Molecular immunology, 2023-12, Vol.164, p.79-87</ispartof><rights>Copyright © 2023 Elsevier Ltd. 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Venom characterization from these scorpions collected on the Pacific coast of Colima, enabled the identification of a second component of medical importance named Ct71 toxin. Amino acid sequence of Ct71 shares a high identity with Chui5 toxin from C. huichol scorpion, which was neutralized by scFv HV. For this reason, the kinetic parameters of interaction between Ct71 toxin and scFv HV were determined by surface plasmon resonance. Results showed a significantly higher affinity for Ct71 as compared to Chui5. As expected, this toxin was neutralized by scFv HV. The injection of a mixture of scFvs HV and RAS27, resulted in the neutralization of C. tecomanus venom, corroborating that human recombinant antibody fragments can efficiently contribute to the neutralization of medically important toxins and their respective venoms from Mexican scorpions.</description><subject>amino acid sequences</subject><subject>Animals</subject><subject>Centruroides</subject><subject>coasts</subject><subject>Humans</subject><subject>Mammals</subject><subject>Mexico</subject><subject>neutralization</subject><subject>recombinant antibodies</subject><subject>Recombinant Proteins - chemistry</subject><subject>Scorpion Venoms</subject><subject>Scorpions</subject><subject>Single-Chain Antibodies</subject><subject>surface plasmon resonance</subject><subject>toxicity</subject><subject>toxins</subject><issn>0161-5890</issn><issn>1872-9142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1P3DAQhq2qFSwf_6CqfOwl6YztfPiIVtAioXKBs2U7TskqjhfbabX99SRa4MphNJfnfWekh5CvCCUC1j92pQ_j4H3JgPESsQTAT2SDbcMKiYJ9JpsFw6JqJZySs5R2AFBDXZ2QU97IFpqGbcj028056nH4r_MQJhp6unVTjnMMQ-cSzc4Gr6c50WRD3K_IXzcFT82B5idH5-TWTP4X6NO8gDSuATNMesp0mcGE7kD7qP_4pTZdkC-9HpO7fN3n5PHm-mH7q7i7_3m7vborLGtlLoyGCrrWGNmBMV1bWa4ROO-BG2eFNoJZy6Uw0lS6RmvbWshGdI2ptbCy5-fk-7F3H8Pz7FJWfkjWjaOeXJiT4lgJrCVrqw_R5SEGiEzyBRVH1MaQUnS92sfB63hQCGqVonbqKEWtUhSiWqQssW-vF2bjXfceerPAXwB3HI0A</recordid><startdate>202312</startdate><enddate>202312</enddate><creator>Valencia-Martínez, Hugo</creator><creator>Riaño-Umbarila, Lidia</creator><creator>Olamendi-Portugal, Timoteo</creator><creator>Romero-Moreno, José Alberto</creator><creator>Possani, Lourival D</creator><creator>Becerril, Baltazar</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-8348-8029</orcidid></search><sort><creationdate>202312</creationdate><title>Neutralization of Centruroides tecomanus scorpion venom by the use of two human recombinant antibody fragments</title><author>Valencia-Martínez, Hugo ; Riaño-Umbarila, Lidia ; Olamendi-Portugal, Timoteo ; Romero-Moreno, José Alberto ; Possani, Lourival D ; Becerril, Baltazar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c289t-ba050d8bb9d0bbd85c3a1033f03bec4ab42cc394b9b5a61cc864974d7b6a4c9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>amino acid sequences</topic><topic>Animals</topic><topic>Centruroides</topic><topic>coasts</topic><topic>Humans</topic><topic>Mammals</topic><topic>Mexico</topic><topic>neutralization</topic><topic>recombinant antibodies</topic><topic>Recombinant Proteins - chemistry</topic><topic>Scorpion Venoms</topic><topic>Scorpions</topic><topic>Single-Chain Antibodies</topic><topic>surface plasmon resonance</topic><topic>toxicity</topic><topic>toxins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valencia-Martínez, Hugo</creatorcontrib><creatorcontrib>Riaño-Umbarila, Lidia</creatorcontrib><creatorcontrib>Olamendi-Portugal, Timoteo</creatorcontrib><creatorcontrib>Romero-Moreno, José Alberto</creatorcontrib><creatorcontrib>Possani, Lourival D</creatorcontrib><creatorcontrib>Becerril, Baltazar</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valencia-Martínez, Hugo</au><au>Riaño-Umbarila, Lidia</au><au>Olamendi-Portugal, Timoteo</au><au>Romero-Moreno, José Alberto</au><au>Possani, Lourival D</au><au>Becerril, Baltazar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutralization of Centruroides tecomanus scorpion venom by the use of two human recombinant antibody fragments</atitle><jtitle>Molecular immunology</jtitle><addtitle>Mol Immunol</addtitle><date>2023-12</date><risdate>2023</risdate><volume>164</volume><spage>79</spage><epage>87</epage><pages>79-87</pages><issn>0161-5890</issn><eissn>1872-9142</eissn><abstract>The first toxic component identified against mammals in the venom from Centruroides tecomanus scorpion from Colima, Mexico was Ct1a toxin, which was neutralized by human single chain variable fragment (scFv) RAS27. Venom characterization from these scorpions collected on the Pacific coast of Colima, enabled the identification of a second component of medical importance named Ct71 toxin. Amino acid sequence of Ct71 shares a high identity with Chui5 toxin from C. huichol scorpion, which was neutralized by scFv HV. For this reason, the kinetic parameters of interaction between Ct71 toxin and scFv HV were determined by surface plasmon resonance. Results showed a significantly higher affinity for Ct71 as compared to Chui5. As expected, this toxin was neutralized by scFv HV. The injection of a mixture of scFvs HV and RAS27, resulted in the neutralization of C. tecomanus venom, corroborating that human recombinant antibody fragments can efficiently contribute to the neutralization of medically important toxins and their respective venoms from Mexican scorpions.</abstract><cop>England</cop><pmid>37980772</pmid><doi>10.1016/j.molimm.2023.11.001</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-8348-8029</orcidid></addata></record> |
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subjects | amino acid sequences Animals Centruroides coasts Humans Mammals Mexico neutralization recombinant antibodies Recombinant Proteins - chemistry Scorpion Venoms Scorpions Single-Chain Antibodies surface plasmon resonance toxicity toxins |
title | Neutralization of Centruroides tecomanus scorpion venom by the use of two human recombinant antibody fragments |
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