Loss of ADP-glucose transporter in barley sex1 mutant caused shrunken endosperm but with elevated protein and β-glucan content in whole meal
Grain shape and plumpness affect barley yield. Despite numerous studies on shrunken endosperm mutants in barley, their molecular mechanism and application potential in the food industry are largely unknown. Here, map-based cloning, co-segregation analyses, and allelic variant validation revealed tha...
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| Veröffentlicht in: | International journal of biological macromolecules 2023-11, Vol.251, p.126365, Article 126365 |
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| creator | Liu, Hang Zou, Yaya Xuan, Qijing Tian, Rong Zhu, Jing Qu, Xiangru Sun, Min Liu, Yanlin Tang, Huaping Deng, Mei Jiang, Qiantao Xu, Qiang Peng, Yuanying Chen, Guoyue Li, Wei Pu, Zhien Jiang, Yunfeng Wang, Jirui Qi, Pengfi Zhang, Yazhou Zheng, Youliang Wei, Yuming Ma, Jian |
| description | Grain shape and plumpness affect barley yield. Despite numerous studies on shrunken endosperm mutants in barley, their molecular mechanism and application potential in the food industry are largely unknown. Here, map-based cloning, co-segregation analyses, and allelic variant validation revealed that the loss of HORVU6Hr1G037950 encoding an ADP-glucose transporter caused the shrunken endosperm in sex1. Haplotype analysis suggested that hap4 in the promoter sequence was positively related to the hundred-grain weight showing a breeding potential. A pair of near-isogenic lines targeting HORVU6Hr1G037950 was produced and characterized to investigate molecular mechanisms that SEX1 regulates endosperm development. Results presented that the absence of the SEX1 gene led to the decrease of starch content and A-type granules size, the increase of β-glucan, protein, gelatinization temperature, soluble sugar content, amylopectin A chains, and B1 chains. Enzymatic activity, transcriptome and metabolome analyses revealed the loss of SEX1 results in an impaired ADP-glucose-to-starch conversion process, consequently leading to higher soluble sugar contents and lower starch accumulation, thereby inducing a shrunken-endosperm phenotype in sex1. Taken together, this study provides new insights into barley grain development, and the elevated protein and β-glucan contents of the whole meal in sex1 imply its promising application in the food industry. |
| doi_str_mv | 10.1016/j.ijbiomac.2023.126365 |
| format | Article |
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Despite numerous studies on shrunken endosperm mutants in barley, their molecular mechanism and application potential in the food industry are largely unknown. Here, map-based cloning, co-segregation analyses, and allelic variant validation revealed that the loss of HORVU6Hr1G037950 encoding an ADP-glucose transporter caused the shrunken endosperm in sex1. Haplotype analysis suggested that hap4 in the promoter sequence was positively related to the hundred-grain weight showing a breeding potential. A pair of near-isogenic lines targeting HORVU6Hr1G037950 was produced and characterized to investigate molecular mechanisms that SEX1 regulates endosperm development. Results presented that the absence of the SEX1 gene led to the decrease of starch content and A-type granules size, the increase of β-glucan, protein, gelatinization temperature, soluble sugar content, amylopectin A chains, and B1 chains. Enzymatic activity, transcriptome and metabolome analyses revealed the loss of SEX1 results in an impaired ADP-glucose-to-starch conversion process, consequently leading to higher soluble sugar contents and lower starch accumulation, thereby inducing a shrunken-endosperm phenotype in sex1. Taken together, this study provides new insights into barley grain development, and the elevated protein and β-glucan contents of the whole meal in sex1 imply its promising application in the food industry.</description><identifier>ISSN: 0141-8130</identifier><identifier>ISSN: 1879-0003</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2023.126365</identifier><identifier>PMID: 37591421</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Alleles ; amylopectin ; barley ; beta-Glucans - metabolism ; endosperm ; Endosperm - genetics ; Endosperm - metabolism ; enzyme activity ; food industry ; gelatinization temperature ; Gene Expression Regulation, Plant ; genes ; haplotypes ; Hordeum - genetics ; Hordeum - metabolism ; metabolome ; mutants ; Mutation ; Phenotype ; Plant Proteins - genetics ; Plant Proteins - metabolism ; promoter regions ; seed development ; Starch - metabolism ; sugar content ; sugars ; transcriptome</subject><ispartof>International journal of biological macromolecules, 2023-11, Vol.251, p.126365, Article 126365</ispartof><rights>Copyright © 2023. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c291t-8182a6264a9f421eb7d00d00a8fc21d3330573d26b9652f7d383c4f08c0ebf643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37591421$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Hang</creatorcontrib><creatorcontrib>Zou, Yaya</creatorcontrib><creatorcontrib>Xuan, Qijing</creatorcontrib><creatorcontrib>Tian, Rong</creatorcontrib><creatorcontrib>Zhu, Jing</creatorcontrib><creatorcontrib>Qu, Xiangru</creatorcontrib><creatorcontrib>Sun, Min</creatorcontrib><creatorcontrib>Liu, Yanlin</creatorcontrib><creatorcontrib>Tang, Huaping</creatorcontrib><creatorcontrib>Deng, Mei</creatorcontrib><creatorcontrib>Jiang, Qiantao</creatorcontrib><creatorcontrib>Xu, Qiang</creatorcontrib><creatorcontrib>Peng, Yuanying</creatorcontrib><creatorcontrib>Chen, Guoyue</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Pu, Zhien</creatorcontrib><creatorcontrib>Jiang, Yunfeng</creatorcontrib><creatorcontrib>Wang, Jirui</creatorcontrib><creatorcontrib>Qi, Pengfi</creatorcontrib><creatorcontrib>Zhang, Yazhou</creatorcontrib><creatorcontrib>Zheng, Youliang</creatorcontrib><creatorcontrib>Wei, Yuming</creatorcontrib><creatorcontrib>Ma, Jian</creatorcontrib><title>Loss of ADP-glucose transporter in barley sex1 mutant caused shrunken endosperm but with elevated protein and β-glucan content in whole meal</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Grain shape and plumpness affect barley yield. Despite numerous studies on shrunken endosperm mutants in barley, their molecular mechanism and application potential in the food industry are largely unknown. Here, map-based cloning, co-segregation analyses, and allelic variant validation revealed that the loss of HORVU6Hr1G037950 encoding an ADP-glucose transporter caused the shrunken endosperm in sex1. Haplotype analysis suggested that hap4 in the promoter sequence was positively related to the hundred-grain weight showing a breeding potential. A pair of near-isogenic lines targeting HORVU6Hr1G037950 was produced and characterized to investigate molecular mechanisms that SEX1 regulates endosperm development. Results presented that the absence of the SEX1 gene led to the decrease of starch content and A-type granules size, the increase of β-glucan, protein, gelatinization temperature, soluble sugar content, amylopectin A chains, and B1 chains. Enzymatic activity, transcriptome and metabolome analyses revealed the loss of SEX1 results in an impaired ADP-glucose-to-starch conversion process, consequently leading to higher soluble sugar contents and lower starch accumulation, thereby inducing a shrunken-endosperm phenotype in sex1. Taken together, this study provides new insights into barley grain development, and the elevated protein and β-glucan contents of the whole meal in sex1 imply its promising application in the food industry.</description><subject>Alleles</subject><subject>amylopectin</subject><subject>barley</subject><subject>beta-Glucans - metabolism</subject><subject>endosperm</subject><subject>Endosperm - genetics</subject><subject>Endosperm - metabolism</subject><subject>enzyme activity</subject><subject>food industry</subject><subject>gelatinization temperature</subject><subject>Gene Expression Regulation, Plant</subject><subject>genes</subject><subject>haplotypes</subject><subject>Hordeum - genetics</subject><subject>Hordeum - metabolism</subject><subject>metabolome</subject><subject>mutants</subject><subject>Mutation</subject><subject>Phenotype</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>promoter regions</subject><subject>seed development</subject><subject>Starch - metabolism</subject><subject>sugar content</subject><subject>sugars</subject><subject>transcriptome</subject><issn>0141-8130</issn><issn>1879-0003</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9O3DAQxq2qVVmgr4B87CWL7Ukc54ho-SOtRA_lbDnOpJttYm9tp8BD8DI8SJ8JLwu9Ilmy5Pnm-2b8I-SEsyVnXJ5ulsOmHfxk7FIwAUsuJMjqA1lwVTcFYww-kgXjJS8UB3ZADmPc5FdZcfWZHEBdNbwUfEEeVz5G6nt69u1H8WucrY9IUzAubn1IGOjgaGvCiA804j2n05yMS9SaOWJH4zrM7jc6iq7zcYthou2c6N2Q1hRH_GtSFm2DT5htjOvov6eXEOOo9S5hdsqFu7UfkU5oxmPyqTdjxC-v9xG5vfj-8_yqWN1cXp-frQorGp7ySkoYKWRpmj5vgW3dMZaPUb0VvAMAVtXQCdk2shJ93YECW_ZMWYZtL0s4Il_3vnm2PzPGpKchWhxH49DPUQOvSl4pgOZdqVAVNCVr1E4q91Ib8qcG7PU2DJMJD5ozvaOmN_qNmt5R03tqufHkNWNuJ-z-t71hgmcJd5fS</recordid><startdate>20231101</startdate><enddate>20231101</enddate><creator>Liu, Hang</creator><creator>Zou, Yaya</creator><creator>Xuan, Qijing</creator><creator>Tian, Rong</creator><creator>Zhu, Jing</creator><creator>Qu, Xiangru</creator><creator>Sun, Min</creator><creator>Liu, Yanlin</creator><creator>Tang, Huaping</creator><creator>Deng, Mei</creator><creator>Jiang, Qiantao</creator><creator>Xu, Qiang</creator><creator>Peng, Yuanying</creator><creator>Chen, Guoyue</creator><creator>Li, Wei</creator><creator>Pu, Zhien</creator><creator>Jiang, Yunfeng</creator><creator>Wang, Jirui</creator><creator>Qi, Pengfi</creator><creator>Zhang, Yazhou</creator><creator>Zheng, Youliang</creator><creator>Wei, Yuming</creator><creator>Ma, Jian</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20231101</creationdate><title>Loss of ADP-glucose transporter in barley sex1 mutant caused shrunken endosperm but with elevated protein and β-glucan content in whole meal</title><author>Liu, Hang ; Zou, Yaya ; Xuan, Qijing ; Tian, Rong ; Zhu, Jing ; Qu, Xiangru ; Sun, Min ; Liu, Yanlin ; Tang, Huaping ; Deng, Mei ; Jiang, Qiantao ; Xu, Qiang ; Peng, Yuanying ; Chen, Guoyue ; Li, Wei ; Pu, Zhien ; Jiang, Yunfeng ; Wang, Jirui ; Qi, Pengfi ; Zhang, Yazhou ; Zheng, Youliang ; Wei, Yuming ; Ma, Jian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c291t-8182a6264a9f421eb7d00d00a8fc21d3330573d26b9652f7d383c4f08c0ebf643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alleles</topic><topic>amylopectin</topic><topic>barley</topic><topic>beta-Glucans - metabolism</topic><topic>endosperm</topic><topic>Endosperm - genetics</topic><topic>Endosperm - metabolism</topic><topic>enzyme activity</topic><topic>food industry</topic><topic>gelatinization temperature</topic><topic>Gene Expression Regulation, Plant</topic><topic>genes</topic><topic>haplotypes</topic><topic>Hordeum - genetics</topic><topic>Hordeum - metabolism</topic><topic>metabolome</topic><topic>mutants</topic><topic>Mutation</topic><topic>Phenotype</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>promoter regions</topic><topic>seed development</topic><topic>Starch - metabolism</topic><topic>sugar content</topic><topic>sugars</topic><topic>transcriptome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Hang</creatorcontrib><creatorcontrib>Zou, Yaya</creatorcontrib><creatorcontrib>Xuan, Qijing</creatorcontrib><creatorcontrib>Tian, Rong</creatorcontrib><creatorcontrib>Zhu, Jing</creatorcontrib><creatorcontrib>Qu, Xiangru</creatorcontrib><creatorcontrib>Sun, Min</creatorcontrib><creatorcontrib>Liu, Yanlin</creatorcontrib><creatorcontrib>Tang, Huaping</creatorcontrib><creatorcontrib>Deng, Mei</creatorcontrib><creatorcontrib>Jiang, Qiantao</creatorcontrib><creatorcontrib>Xu, Qiang</creatorcontrib><creatorcontrib>Peng, Yuanying</creatorcontrib><creatorcontrib>Chen, Guoyue</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Pu, Zhien</creatorcontrib><creatorcontrib>Jiang, Yunfeng</creatorcontrib><creatorcontrib>Wang, Jirui</creatorcontrib><creatorcontrib>Qi, Pengfi</creatorcontrib><creatorcontrib>Zhang, Yazhou</creatorcontrib><creatorcontrib>Zheng, Youliang</creatorcontrib><creatorcontrib>Wei, Yuming</creatorcontrib><creatorcontrib>Ma, Jian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Hang</au><au>Zou, Yaya</au><au>Xuan, Qijing</au><au>Tian, Rong</au><au>Zhu, Jing</au><au>Qu, Xiangru</au><au>Sun, Min</au><au>Liu, Yanlin</au><au>Tang, Huaping</au><au>Deng, Mei</au><au>Jiang, Qiantao</au><au>Xu, Qiang</au><au>Peng, Yuanying</au><au>Chen, Guoyue</au><au>Li, Wei</au><au>Pu, Zhien</au><au>Jiang, Yunfeng</au><au>Wang, Jirui</au><au>Qi, Pengfi</au><au>Zhang, Yazhou</au><au>Zheng, Youliang</au><au>Wei, Yuming</au><au>Ma, Jian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Loss of ADP-glucose transporter in barley sex1 mutant caused shrunken endosperm but with elevated protein and β-glucan content in whole meal</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2023-11-01</date><risdate>2023</risdate><volume>251</volume><spage>126365</spage><pages>126365-</pages><artnum>126365</artnum><issn>0141-8130</issn><issn>1879-0003</issn><eissn>1879-0003</eissn><abstract>Grain shape and plumpness affect barley yield. Despite numerous studies on shrunken endosperm mutants in barley, their molecular mechanism and application potential in the food industry are largely unknown. Here, map-based cloning, co-segregation analyses, and allelic variant validation revealed that the loss of HORVU6Hr1G037950 encoding an ADP-glucose transporter caused the shrunken endosperm in sex1. Haplotype analysis suggested that hap4 in the promoter sequence was positively related to the hundred-grain weight showing a breeding potential. A pair of near-isogenic lines targeting HORVU6Hr1G037950 was produced and characterized to investigate molecular mechanisms that SEX1 regulates endosperm development. Results presented that the absence of the SEX1 gene led to the decrease of starch content and A-type granules size, the increase of β-glucan, protein, gelatinization temperature, soluble sugar content, amylopectin A chains, and B1 chains. Enzymatic activity, transcriptome and metabolome analyses revealed the loss of SEX1 results in an impaired ADP-glucose-to-starch conversion process, consequently leading to higher soluble sugar contents and lower starch accumulation, thereby inducing a shrunken-endosperm phenotype in sex1. Taken together, this study provides new insights into barley grain development, and the elevated protein and β-glucan contents of the whole meal in sex1 imply its promising application in the food industry.</abstract><cop>Netherlands</cop><pmid>37591421</pmid><doi>10.1016/j.ijbiomac.2023.126365</doi></addata></record> |
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| subjects | Alleles amylopectin barley beta-Glucans - metabolism endosperm Endosperm - genetics Endosperm - metabolism enzyme activity food industry gelatinization temperature Gene Expression Regulation, Plant genes haplotypes Hordeum - genetics Hordeum - metabolism metabolome mutants Mutation Phenotype Plant Proteins - genetics Plant Proteins - metabolism promoter regions seed development Starch - metabolism sugar content sugars transcriptome |
| title | Loss of ADP-glucose transporter in barley sex1 mutant caused shrunken endosperm but with elevated protein and β-glucan content in whole meal |
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