Combination effects of ultrasound and citral nanoemulsion against Shigella flexneri and the preservation effect on fresh-cut carrots
This present study evaluated the effect of ultrasound (US), citral nanoemulsion (CLON) and combined treatments on the inactivate effectiveness of Shigella flexneri in phosphate-buffered saline (PBS) and on the surface of fresh-cut carrots. In this study, CLON was produced by ultrasonic nanoemulsific...
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| Veröffentlicht in: | Food control 2024-01, Vol.155, p.110069, Article 110069 |
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| creator | Song, Luyi Yang, Hui Cheng, Shuai Zhang, Zhen Zhang, Lechi Su, Ruiying Li, Yuxi Zhan, Xiangjun Yang, Baowei Lin, Lin Shi, Chao |
| description | This present study evaluated the effect of ultrasound (US), citral nanoemulsion (CLON) and combined treatments on the inactivate effectiveness of Shigella flexneri in phosphate-buffered saline (PBS) and on the surface of fresh-cut carrots. In this study, CLON was produced by ultrasonic nanoemulsification. Results demonstrated that US + CLON treatment significantly (P |
| doi_str_mv | 10.1016/j.foodcont.2023.110069 |
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| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3154156737</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0956713523004693</els_id><sourcerecordid>3154156737</sourcerecordid><originalsourceid>FETCH-LOGICAL-c345t-da6c07251a83eaae20a55bc7b1c2152ff174d754acfd795fe898e8dcbe0003773</originalsourceid><addsrcrecordid>eNqFUD1PwzAU9AASpfAXkEeWBDuJ42QDVXxJlRiA2XKc59ZVYhfbqWDnh5MQkNgYnt6H7k7vDqELSlJKaHm1S7VzrXI2phnJ8pRSQsr6CC1IzcqE05ydoNMQdoRQTihZoM-V6xtjZTTOYtAaVAzYaTx00cvgBttiOZYy49phK62DfujChJYbaWyI-HlrNtB1EusO3i14882IW8B7DwH84a84Hic9nreJGiJW0nsXwxk61rILcP7Tl-j17vZl9ZCsn-4fVzfrROUFi0krS0V4xqiscpASMiIZaxRvqMooy7SmvGg5K6TSLa-ZhqquoGpVA4SQnPN8iS5n3b13bwOEKHoT1PS7BTcEkVNWUFbyfIKWM1R5F4IHLfbe9NJ_CErEFLXYid-oxRS1mKMeidczEUYjBwNeBGXAKmiNH_2L1pn_JL4ABJ6RAA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3154156737</pqid></control><display><type>article</type><title>Combination effects of ultrasound and citral nanoemulsion against Shigella flexneri and the preservation effect on fresh-cut carrots</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Song, Luyi ; Yang, Hui ; Cheng, Shuai ; Zhang, Zhen ; Zhang, Lechi ; Su, Ruiying ; Li, Yuxi ; Zhan, Xiangjun ; Yang, Baowei ; Lin, Lin ; Shi, Chao</creator><creatorcontrib>Song, Luyi ; Yang, Hui ; Cheng, Shuai ; Zhang, Zhen ; Zhang, Lechi ; Su, Ruiying ; Li, Yuxi ; Zhan, Xiangjun ; Yang, Baowei ; Lin, Lin ; Shi, Chao</creatorcontrib><description>This present study evaluated the effect of ultrasound (US), citral nanoemulsion (CLON) and combined treatments on the inactivate effectiveness of Shigella flexneri in phosphate-buffered saline (PBS) and on the surface of fresh-cut carrots. In this study, CLON was produced by ultrasonic nanoemulsification. Results demonstrated that US + CLON treatment significantly (P < 0.05) improved the bactericidal effect against Sh. flexneri, especially 0.15 mg/mL of CLON combined with US (20 kHz, 345 W/cm2) treatment for 9 min decreased the number of Sh. flexneri by 8.55 log CFU/mL. The assay of reactive oxygen species (ROS) and malondialdehyde (MDA) levels indicated that US + CLON aggravated oxidative stress and lipid peroxidation in cell membranes. The leakage of cell constituents (proteins and nucleotide) demonstrated that the permeability and integrity of the cell membrane was significantly (P < 0.05) impaired. Field-emission scanning electron microscopy further showed that US + CLON exacerbates the disruption of the bacteria morphology. Confocal laser scanning microscopic and flow cytometry observed US + CLON treatment enhanced the changes of bacterial cell membrane integrity. Meanwhile, US + CLON was effective in reducing the presence of bacteria on the surface of fresh-cut carrots, and had no negative effects on the quality of fresh-cut carrots in terms of colour, hardness and weight during storage. Overall, this present study revealed ultrasound combined with CLON is a promising and viable cleaning method for improving the microbiological safety of fresh produce.
[Display omitted]
•Ultrasound (US) and citral nanoemulsion (CLON) had synergistic antibacterial effects against Sh. flexneri.•US combined with CLON significantly damaged the permeability and integrity of cell membrane.•US combined with CLON increased the levels of radicalized oxidative stress and lipid peroxidation of bacterial cells.•US combined with CLON reduced the amount of Sh. flexneri attached to the surface of fresh-cut carrots.•US combined with CLON maintained important quality-related attributes of fresh-cut carrots.</description><identifier>ISSN: 0956-7135</identifier><identifier>DOI: 10.1016/j.foodcont.2023.110069</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Antibacterial activity ; antibacterial properties ; bacteria ; Cell membrane ; cell membranes ; citral ; Citral nanoemulsion ; color ; electron microscopy ; emulsifying ; flow cytometry ; food safety ; fresh produce ; Fresh-cut carrots ; hardness ; lipid peroxidation ; malondialdehyde ; microbiological quality ; nanoemulsions ; oxidative stress ; permeability ; reactive oxygen species ; Shigella flexneri ; ultrasonics ; Ultrasound</subject><ispartof>Food control, 2024-01, Vol.155, p.110069, Article 110069</ispartof><rights>2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-da6c07251a83eaae20a55bc7b1c2152ff174d754acfd795fe898e8dcbe0003773</citedby><cites>FETCH-LOGICAL-c345t-da6c07251a83eaae20a55bc7b1c2152ff174d754acfd795fe898e8dcbe0003773</cites><orcidid>0000-0001-6374-2165 ; 0000-0003-3950-4268</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956713523004693$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids></links><search><creatorcontrib>Song, Luyi</creatorcontrib><creatorcontrib>Yang, Hui</creatorcontrib><creatorcontrib>Cheng, Shuai</creatorcontrib><creatorcontrib>Zhang, Zhen</creatorcontrib><creatorcontrib>Zhang, Lechi</creatorcontrib><creatorcontrib>Su, Ruiying</creatorcontrib><creatorcontrib>Li, Yuxi</creatorcontrib><creatorcontrib>Zhan, Xiangjun</creatorcontrib><creatorcontrib>Yang, Baowei</creatorcontrib><creatorcontrib>Lin, Lin</creatorcontrib><creatorcontrib>Shi, Chao</creatorcontrib><title>Combination effects of ultrasound and citral nanoemulsion against Shigella flexneri and the preservation effect on fresh-cut carrots</title><title>Food control</title><description>This present study evaluated the effect of ultrasound (US), citral nanoemulsion (CLON) and combined treatments on the inactivate effectiveness of Shigella flexneri in phosphate-buffered saline (PBS) and on the surface of fresh-cut carrots. In this study, CLON was produced by ultrasonic nanoemulsification. Results demonstrated that US + CLON treatment significantly (P < 0.05) improved the bactericidal effect against Sh. flexneri, especially 0.15 mg/mL of CLON combined with US (20 kHz, 345 W/cm2) treatment for 9 min decreased the number of Sh. flexneri by 8.55 log CFU/mL. The assay of reactive oxygen species (ROS) and malondialdehyde (MDA) levels indicated that US + CLON aggravated oxidative stress and lipid peroxidation in cell membranes. The leakage of cell constituents (proteins and nucleotide) demonstrated that the permeability and integrity of the cell membrane was significantly (P < 0.05) impaired. Field-emission scanning electron microscopy further showed that US + CLON exacerbates the disruption of the bacteria morphology. Confocal laser scanning microscopic and flow cytometry observed US + CLON treatment enhanced the changes of bacterial cell membrane integrity. Meanwhile, US + CLON was effective in reducing the presence of bacteria on the surface of fresh-cut carrots, and had no negative effects on the quality of fresh-cut carrots in terms of colour, hardness and weight during storage. Overall, this present study revealed ultrasound combined with CLON is a promising and viable cleaning method for improving the microbiological safety of fresh produce.
[Display omitted]
•Ultrasound (US) and citral nanoemulsion (CLON) had synergistic antibacterial effects against Sh. flexneri.•US combined with CLON significantly damaged the permeability and integrity of cell membrane.•US combined with CLON increased the levels of radicalized oxidative stress and lipid peroxidation of bacterial cells.•US combined with CLON reduced the amount of Sh. flexneri attached to the surface of fresh-cut carrots.•US combined with CLON maintained important quality-related attributes of fresh-cut carrots.</description><subject>Antibacterial activity</subject><subject>antibacterial properties</subject><subject>bacteria</subject><subject>Cell membrane</subject><subject>cell membranes</subject><subject>citral</subject><subject>Citral nanoemulsion</subject><subject>color</subject><subject>electron microscopy</subject><subject>emulsifying</subject><subject>flow cytometry</subject><subject>food safety</subject><subject>fresh produce</subject><subject>Fresh-cut carrots</subject><subject>hardness</subject><subject>lipid peroxidation</subject><subject>malondialdehyde</subject><subject>microbiological quality</subject><subject>nanoemulsions</subject><subject>oxidative stress</subject><subject>permeability</subject><subject>reactive oxygen species</subject><subject>Shigella flexneri</subject><subject>ultrasonics</subject><subject>Ultrasound</subject><issn>0956-7135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFUD1PwzAU9AASpfAXkEeWBDuJ42QDVXxJlRiA2XKc59ZVYhfbqWDnh5MQkNgYnt6H7k7vDqELSlJKaHm1S7VzrXI2phnJ8pRSQsr6CC1IzcqE05ydoNMQdoRQTihZoM-V6xtjZTTOYtAaVAzYaTx00cvgBttiOZYy49phK62DfujChJYbaWyI-HlrNtB1EusO3i14882IW8B7DwH84a84Hic9nreJGiJW0nsXwxk61rILcP7Tl-j17vZl9ZCsn-4fVzfrROUFi0krS0V4xqiscpASMiIZaxRvqMooy7SmvGg5K6TSLa-ZhqquoGpVA4SQnPN8iS5n3b13bwOEKHoT1PS7BTcEkVNWUFbyfIKWM1R5F4IHLfbe9NJ_CErEFLXYid-oxRS1mKMeidczEUYjBwNeBGXAKmiNH_2L1pn_JL4ABJ6RAA</recordid><startdate>202401</startdate><enddate>202401</enddate><creator>Song, Luyi</creator><creator>Yang, Hui</creator><creator>Cheng, Shuai</creator><creator>Zhang, Zhen</creator><creator>Zhang, Lechi</creator><creator>Su, Ruiying</creator><creator>Li, Yuxi</creator><creator>Zhan, Xiangjun</creator><creator>Yang, Baowei</creator><creator>Lin, Lin</creator><creator>Shi, Chao</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-6374-2165</orcidid><orcidid>https://orcid.org/0000-0003-3950-4268</orcidid></search><sort><creationdate>202401</creationdate><title>Combination effects of ultrasound and citral nanoemulsion against Shigella flexneri and the preservation effect on fresh-cut carrots</title><author>Song, Luyi ; Yang, Hui ; Cheng, Shuai ; Zhang, Zhen ; Zhang, Lechi ; Su, Ruiying ; Li, Yuxi ; Zhan, Xiangjun ; Yang, Baowei ; Lin, Lin ; Shi, Chao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c345t-da6c07251a83eaae20a55bc7b1c2152ff174d754acfd795fe898e8dcbe0003773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antibacterial activity</topic><topic>antibacterial properties</topic><topic>bacteria</topic><topic>Cell membrane</topic><topic>cell membranes</topic><topic>citral</topic><topic>Citral nanoemulsion</topic><topic>color</topic><topic>electron microscopy</topic><topic>emulsifying</topic><topic>flow cytometry</topic><topic>food safety</topic><topic>fresh produce</topic><topic>Fresh-cut carrots</topic><topic>hardness</topic><topic>lipid peroxidation</topic><topic>malondialdehyde</topic><topic>microbiological quality</topic><topic>nanoemulsions</topic><topic>oxidative stress</topic><topic>permeability</topic><topic>reactive oxygen species</topic><topic>Shigella flexneri</topic><topic>ultrasonics</topic><topic>Ultrasound</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Luyi</creatorcontrib><creatorcontrib>Yang, Hui</creatorcontrib><creatorcontrib>Cheng, Shuai</creatorcontrib><creatorcontrib>Zhang, Zhen</creatorcontrib><creatorcontrib>Zhang, Lechi</creatorcontrib><creatorcontrib>Su, Ruiying</creatorcontrib><creatorcontrib>Li, Yuxi</creatorcontrib><creatorcontrib>Zhan, Xiangjun</creatorcontrib><creatorcontrib>Yang, Baowei</creatorcontrib><creatorcontrib>Lin, Lin</creatorcontrib><creatorcontrib>Shi, Chao</creatorcontrib><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Food control</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Luyi</au><au>Yang, Hui</au><au>Cheng, Shuai</au><au>Zhang, Zhen</au><au>Zhang, Lechi</au><au>Su, Ruiying</au><au>Li, Yuxi</au><au>Zhan, Xiangjun</au><au>Yang, Baowei</au><au>Lin, Lin</au><au>Shi, Chao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combination effects of ultrasound and citral nanoemulsion against Shigella flexneri and the preservation effect on fresh-cut carrots</atitle><jtitle>Food control</jtitle><date>2024-01</date><risdate>2024</risdate><volume>155</volume><spage>110069</spage><pages>110069-</pages><artnum>110069</artnum><issn>0956-7135</issn><abstract>This present study evaluated the effect of ultrasound (US), citral nanoemulsion (CLON) and combined treatments on the inactivate effectiveness of Shigella flexneri in phosphate-buffered saline (PBS) and on the surface of fresh-cut carrots. In this study, CLON was produced by ultrasonic nanoemulsification. Results demonstrated that US + CLON treatment significantly (P < 0.05) improved the bactericidal effect against Sh. flexneri, especially 0.15 mg/mL of CLON combined with US (20 kHz, 345 W/cm2) treatment for 9 min decreased the number of Sh. flexneri by 8.55 log CFU/mL. The assay of reactive oxygen species (ROS) and malondialdehyde (MDA) levels indicated that US + CLON aggravated oxidative stress and lipid peroxidation in cell membranes. The leakage of cell constituents (proteins and nucleotide) demonstrated that the permeability and integrity of the cell membrane was significantly (P < 0.05) impaired. Field-emission scanning electron microscopy further showed that US + CLON exacerbates the disruption of the bacteria morphology. Confocal laser scanning microscopic and flow cytometry observed US + CLON treatment enhanced the changes of bacterial cell membrane integrity. Meanwhile, US + CLON was effective in reducing the presence of bacteria on the surface of fresh-cut carrots, and had no negative effects on the quality of fresh-cut carrots in terms of colour, hardness and weight during storage. Overall, this present study revealed ultrasound combined with CLON is a promising and viable cleaning method for improving the microbiological safety of fresh produce.
[Display omitted]
•Ultrasound (US) and citral nanoemulsion (CLON) had synergistic antibacterial effects against Sh. flexneri.•US combined with CLON significantly damaged the permeability and integrity of cell membrane.•US combined with CLON increased the levels of radicalized oxidative stress and lipid peroxidation of bacterial cells.•US combined with CLON reduced the amount of Sh. flexneri attached to the surface of fresh-cut carrots.•US combined with CLON maintained important quality-related attributes of fresh-cut carrots.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.foodcont.2023.110069</doi><orcidid>https://orcid.org/0000-0001-6374-2165</orcidid><orcidid>https://orcid.org/0000-0003-3950-4268</orcidid></addata></record> |
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| subjects | Antibacterial activity antibacterial properties bacteria Cell membrane cell membranes citral Citral nanoemulsion color electron microscopy emulsifying flow cytometry food safety fresh produce Fresh-cut carrots hardness lipid peroxidation malondialdehyde microbiological quality nanoemulsions oxidative stress permeability reactive oxygen species Shigella flexneri ultrasonics Ultrasound |
| title | Combination effects of ultrasound and citral nanoemulsion against Shigella flexneri and the preservation effect on fresh-cut carrots |
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