Surgical Face Mask as an Air Sampling Device for Assessing Personal Exposure to Airborne Antimicrobial Resistance Gene-Bearing Bacteria
In this study, we assessed the feasibility of using a surgical face mask as a sampling device to collect airborne antimicrobial resistance genes (ARGs). The method entails collection of ARG-bearing microbes on face masks, followed by their DNA extraction and quantification by qPCR analysis. Analysis...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2024-10, Vol.96 (42), p.17021-17026 |
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| creator | Ham, Yat-Hing Cheng, Jinping Nagl, Stefan Yu, Jian Zhen Cai, Zongwei Chan, Wan |
| description | In this study, we assessed the feasibility of using a surgical face mask as a sampling device to collect airborne antimicrobial resistance genes (ARGs). The method entails collection of ARG-bearing microbes on face masks, followed by their DNA extraction and quantification by qPCR analysis. Analysis of masks worn by volunteers showed an apparent mask wearing time-dependent accumulation of 16S rRNA gene and select ARGs trapped on masks, highlighting the applicability of the method in monitoring personal ARG exposure through inhalation. The sampling method was then validated for reproducibility and compared with a filter-based sampling method before application in different environmental settings to further assess personal exposure to ARGs. In comparison with the filter-based method, our new sampling method does not require a sampling pump and is more user-friendly. More importantly, it records ARG exposure down to the personalized level; thus, it may be used in routine monitoring of occupational exposure and surveillance of ARG concentrations in indoor environments. |
| doi_str_mv | 10.1021/acs.analchem.4c04703 |
| format | Article |
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The method entails collection of ARG-bearing microbes on face masks, followed by their DNA extraction and quantification by qPCR analysis. Analysis of masks worn by volunteers showed an apparent mask wearing time-dependent accumulation of 16S rRNA gene and select ARGs trapped on masks, highlighting the applicability of the method in monitoring personal ARG exposure through inhalation. The sampling method was then validated for reproducibility and compared with a filter-based sampling method before application in different environmental settings to further assess personal exposure to ARGs. In comparison with the filter-based method, our new sampling method does not require a sampling pump and is more user-friendly. More importantly, it records ARG exposure down to the personalized level; thus, it may be used in routine monitoring of occupational exposure and surveillance of ARG concentrations in indoor environments.</description><identifier>ISSN: 0003-2700</identifier><identifier>ISSN: 1520-6882</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.4c04703</identifier><identifier>PMID: 39394980</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>air ; Air sampling ; analytical chemistry ; antibiotic resistance ; Antimicrobial agents ; Antimicrobial resistance ; DNA ; Drug resistance ; Environmental monitoring ; Exposure ; Face ; face masks ; Feasibility studies ; genes ; Indoor environments ; Inhalation ; Masks ; Monitoring ; Occupational exposure ; Occupational health ; Protective equipment ; Respiration ; rRNA 16S ; Samplers ; Sampling methods</subject><ispartof>Analytical chemistry (Washington), 2024-10, Vol.96 (42), p.17021-17026</ispartof><rights>2024 American Chemical Society</rights><rights>Copyright American Chemical Society Oct 22, 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a288t-8563a300fc81b96ee892350dc2d192d2497dcb8c9418a3f3b7a3949013acad93</cites><orcidid>0000-0002-6165-6500 ; 0000-0001-8479-3172 ; 0000-0001-5512-1174 ; 0000-0002-8724-7684 ; 0000-0003-3585-6645</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.4c04703$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.4c04703$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39394980$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ham, Yat-Hing</creatorcontrib><creatorcontrib>Cheng, Jinping</creatorcontrib><creatorcontrib>Nagl, Stefan</creatorcontrib><creatorcontrib>Yu, Jian Zhen</creatorcontrib><creatorcontrib>Cai, Zongwei</creatorcontrib><creatorcontrib>Chan, Wan</creatorcontrib><title>Surgical Face Mask as an Air Sampling Device for Assessing Personal Exposure to Airborne Antimicrobial Resistance Gene-Bearing Bacteria</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>In this study, we assessed the feasibility of using a surgical face mask as a sampling device to collect airborne antimicrobial resistance genes (ARGs). The method entails collection of ARG-bearing microbes on face masks, followed by their DNA extraction and quantification by qPCR analysis. Analysis of masks worn by volunteers showed an apparent mask wearing time-dependent accumulation of 16S rRNA gene and select ARGs trapped on masks, highlighting the applicability of the method in monitoring personal ARG exposure through inhalation. The sampling method was then validated for reproducibility and compared with a filter-based sampling method before application in different environmental settings to further assess personal exposure to ARGs. In comparison with the filter-based method, our new sampling method does not require a sampling pump and is more user-friendly. 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Cheng, Jinping ; Nagl, Stefan ; Yu, Jian Zhen ; Cai, Zongwei ; Chan, Wan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a288t-8563a300fc81b96ee892350dc2d192d2497dcb8c9418a3f3b7a3949013acad93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>air</topic><topic>Air sampling</topic><topic>analytical chemistry</topic><topic>antibiotic resistance</topic><topic>Antimicrobial agents</topic><topic>Antimicrobial resistance</topic><topic>DNA</topic><topic>Drug resistance</topic><topic>Environmental monitoring</topic><topic>Exposure</topic><topic>Face</topic><topic>face masks</topic><topic>Feasibility studies</topic><topic>genes</topic><topic>Indoor environments</topic><topic>Inhalation</topic><topic>Masks</topic><topic>Monitoring</topic><topic>Occupational exposure</topic><topic>Occupational health</topic><topic>Protective equipment</topic><topic>Respiration</topic><topic>rRNA 16S</topic><topic>Samplers</topic><topic>Sampling methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ham, Yat-Hing</creatorcontrib><creatorcontrib>Cheng, Jinping</creatorcontrib><creatorcontrib>Nagl, Stefan</creatorcontrib><creatorcontrib>Yu, Jian Zhen</creatorcontrib><creatorcontrib>Cai, Zongwei</creatorcontrib><creatorcontrib>Chan, Wan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ham, Yat-Hing</au><au>Cheng, Jinping</au><au>Nagl, Stefan</au><au>Yu, Jian Zhen</au><au>Cai, Zongwei</au><au>Chan, Wan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surgical Face Mask as an Air Sampling Device for Assessing Personal Exposure to Airborne Antimicrobial Resistance Gene-Bearing Bacteria</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. 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| source | ACS Publications |
| subjects | air Air sampling analytical chemistry antibiotic resistance Antimicrobial agents Antimicrobial resistance DNA Drug resistance Environmental monitoring Exposure Face face masks Feasibility studies genes Indoor environments Inhalation Masks Monitoring Occupational exposure Occupational health Protective equipment Respiration rRNA 16S Samplers Sampling methods |
| title | Surgical Face Mask as an Air Sampling Device for Assessing Personal Exposure to Airborne Antimicrobial Resistance Gene-Bearing Bacteria |
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