2D-CEX–FcRn–MS to Study Structure/Function Relation of mAb Charge Variants

The automated elucidation of the interplay between monoclonal antibody (mAb) structure and function using two-dimensional liquid chromatography–mass spectrometry (2D-LC–MS) is reported. Charge variants, induced through forced degradation, are resolved by first-dimension (1D) cation-exchange chromato...

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Veröffentlicht in:	Analytical chemistry (Washington) 2024-11, Vol.96 (45), p.18122-18131
Hauptverfasser:	Verscheure, Liesa, Vandenheede, Isabel, De Rore, Eline, Meersseman, Mabelle, Hanssens, Valerie, Meerschaert, Kris, Stals, Hilde, Sandra, Pat, Lynen, Frederic, Borgions, Filip, Sandra, Koen
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Affinity chromatography analytical chemistry Antibodies, Monoclonal - analysis Antibodies, Monoclonal - chemistry automation Binding Cation exchanging Cation-exchange chromatography Chromatography Chromatography, Ion Exchange - methods Chromatography, Liquid - methods deamidation Drug development Enzyme-linked immunosorbent assay Glycosylation Histocompatibility Antigens Class I - chemistry Histocompatibility Antigens Class I - metabolism Humans Liquid chromatography Mass Spectrometry Mass spectroscopy Monoclonal antibodies Neonates Oxidation Receptors, Fc - chemistry Receptors, Fc - metabolism Sample preparation Structure-Activity Relationship Structure-function relationships therapeutics Workflow
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