Isolation and Characterization of Antimicrobial Peptides Isolated from Brevibacillus halotolerans 7WMA2 for the Activity Against Multidrug-Resistant Pathogens
Multidrug resistance to pathogens has posed a severe threat to public health. The threat could be addressed by antimicrobial peptides (AMPs) with broad-spectrum suppression. In this study, Brevibacillus halotolerans 7WMA2, isolated from marine sediment, produced AMPs against Gram-positive and Gram-n...
Gespeichert in:
| Veröffentlicht in: | Molecular biotechnology 2024-12, Vol.66 (12), p.3618-3627 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3627 |
|---|---|
| container_issue | 12 |
| container_start_page | 3618 |
| container_title | Molecular biotechnology |
| container_volume | 66 |
| creator | Le Han, Ho Pham, Phu Tran Vinh Kim, Song-Gun Chan, Sook Sin Khoo, Kuan Shiong Chew, Kit Wayne Show, Pau Loke Tran, Thi Ngoc Thu Nguyen, Hai Thi Viet Nguyen, Phuong Thi Dong |
| description | Multidrug resistance to pathogens has posed a severe threat to public health. The threat could be addressed by antimicrobial peptides (AMPs) with broad-spectrum suppression. In this study,
Brevibacillus halotolerans
7WMA2, isolated from marine sediment, produced AMPs against Gram-positive and Gram-negative bacteria. The AMPs were precipitated by ammonium sulfate 30% (w/v) from culture broth and dialyzed by a 1 kDa membrane. Tryptone Soy Agar (TSA) was used for the cultivation and resulted in the largest bacteria-inhibiting zones under aerobic conditions at 25 °C, 48 h. An SDS-PAGE gel overlay test revealed that strain 7WMA2 could produce AMPs of 5–10 kDa and showed no degradation when held at 121 °C for 30 min at a wide pH 2–12 range. The AMPs did not cause toxicity to HeLa cells with concentrations up to 500 µg/mL while increasing the arbitrary unit up to eight times. The study showed that the AMPs produced were unique, with broad-spectrum antimicrobial ability.
Graphical Abstract |
| doi_str_mv | 10.1007/s12033-023-00963-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3154149478</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2896805869</sourcerecordid><originalsourceid>FETCH-LOGICAL-c359t-3d4e66ff5da901c19d956e0fc582b35732b0d9a7ead4d5e1efd97d3a1192a97c3</originalsourceid><addsrcrecordid>eNqFkctu1TAQhiMEohd4ARbIEhs2AV_iOF6Go0IrtaJCIJbRJJ6c48rHPthOpfIwPCuGFJBYwMIXjb__t2b-qnrG6CtGqXqdGKdC1JSXRXVb9gfVMZNS11RQ-bDcqRJ1Szt5VJ2kdEMpZ7IRj6sj0dGGK6mOq28XKTjINngC3pDNDiJMGaP9uhbDTHqf7d5OMYwWHLnGQ7YGE1mFaMgcw568iXhrR5isc0siO3AhB4cRfCLq81XPyRwiyTsk_ZTtrc13pN-C9SmTq8UVw7hs6w-YbMrgM7mGvAtb9OlJ9WgGl_Dp_XlafXp79nFzXl--f3ex6S_rSUida2EabNt5lgY0ZRPTRssW6TzJjo9CKsFHajQoBNMYiQxno5URwJjmoNUkTquXq-8hhi8LpjzsbZrQOfAYljSIMjjW6EZ1_0V5p9uOyq7VBX3xF3oTluhLI8WQd02jecsLxVeqjDiliPNwiHYP8W5gdPgR9LAGPZSgh59BD7SInt9bL-MezW_Jr2QLIFYglSe_xfjn73_Yfgc7n7X6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3128449262</pqid></control><display><type>article</type><title>Isolation and Characterization of Antimicrobial Peptides Isolated from Brevibacillus halotolerans 7WMA2 for the Activity Against Multidrug-Resistant Pathogens</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Le Han, Ho ; Pham, Phu Tran Vinh ; Kim, Song-Gun ; Chan, Sook Sin ; Khoo, Kuan Shiong ; Chew, Kit Wayne ; Show, Pau Loke ; Tran, Thi Ngoc Thu ; Nguyen, Hai Thi Viet ; Nguyen, Phuong Thi Dong</creator><creatorcontrib>Le Han, Ho ; Pham, Phu Tran Vinh ; Kim, Song-Gun ; Chan, Sook Sin ; Khoo, Kuan Shiong ; Chew, Kit Wayne ; Show, Pau Loke ; Tran, Thi Ngoc Thu ; Nguyen, Hai Thi Viet ; Nguyen, Phuong Thi Dong</creatorcontrib><description>Multidrug resistance to pathogens has posed a severe threat to public health. The threat could be addressed by antimicrobial peptides (AMPs) with broad-spectrum suppression. In this study,
Brevibacillus halotolerans
7WMA2, isolated from marine sediment, produced AMPs against Gram-positive and Gram-negative bacteria. The AMPs were precipitated by ammonium sulfate 30% (w/v) from culture broth and dialyzed by a 1 kDa membrane. Tryptone Soy Agar (TSA) was used for the cultivation and resulted in the largest bacteria-inhibiting zones under aerobic conditions at 25 °C, 48 h. An SDS-PAGE gel overlay test revealed that strain 7WMA2 could produce AMPs of 5–10 kDa and showed no degradation when held at 121 °C for 30 min at a wide pH 2–12 range. The AMPs did not cause toxicity to HeLa cells with concentrations up to 500 µg/mL while increasing the arbitrary unit up to eight times. The study showed that the AMPs produced were unique, with broad-spectrum antimicrobial ability.
Graphical Abstract</description><identifier>ISSN: 1073-6085</identifier><identifier>ISSN: 1559-0305</identifier><identifier>EISSN: 1559-0305</identifier><identifier>DOI: 10.1007/s12033-023-00963-0</identifier><identifier>PMID: 38042757</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aerobic conditions ; agar ; Ammonium ; Ammonium sulfate ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - isolation & purification ; Anti-Bacterial Agents - pharmacology ; Antimicrobial peptides ; Antimicrobial Peptides - chemistry ; Antimicrobial Peptides - isolation & purification ; Antimicrobial Peptides - pharmacology ; Bacteria ; Biochemistry ; Biological Techniques ; Biotechnology ; Brevibacillus ; Cell Biology ; Cell culture ; Chemistry ; Chemistry and Materials Science ; culture media ; Drug resistance ; Drug Resistance, Multiple, Bacterial - drug effects ; Gram-negative bacteria ; Gram-Negative Bacteria - drug effects ; Gram-positive bacteria ; Gram-Positive Bacteria - drug effects ; HeLa Cells ; Human Genetics ; Humans ; Marine sediments ; Microbial Sensitivity Tests ; Multidrug resistance ; Multidrug resistant organisms ; multiple drug resistance ; Original Paper ; Pathogens ; Peptides ; polyacrylamide gel electrophoresis ; Protein Science ; Public health ; Toxicity ; tryptones</subject><ispartof>Molecular biotechnology, 2024-12, Vol.66 (12), p.3618-3627</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-3d4e66ff5da901c19d956e0fc582b35732b0d9a7ead4d5e1efd97d3a1192a97c3</cites><orcidid>0000-0002-5369-2675</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12033-023-00963-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12033-023-00963-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38042757$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Le Han, Ho</creatorcontrib><creatorcontrib>Pham, Phu Tran Vinh</creatorcontrib><creatorcontrib>Kim, Song-Gun</creatorcontrib><creatorcontrib>Chan, Sook Sin</creatorcontrib><creatorcontrib>Khoo, Kuan Shiong</creatorcontrib><creatorcontrib>Chew, Kit Wayne</creatorcontrib><creatorcontrib>Show, Pau Loke</creatorcontrib><creatorcontrib>Tran, Thi Ngoc Thu</creatorcontrib><creatorcontrib>Nguyen, Hai Thi Viet</creatorcontrib><creatorcontrib>Nguyen, Phuong Thi Dong</creatorcontrib><title>Isolation and Characterization of Antimicrobial Peptides Isolated from Brevibacillus halotolerans 7WMA2 for the Activity Against Multidrug-Resistant Pathogens</title><title>Molecular biotechnology</title><addtitle>Mol Biotechnol</addtitle><addtitle>Mol Biotechnol</addtitle><description>Multidrug resistance to pathogens has posed a severe threat to public health. The threat could be addressed by antimicrobial peptides (AMPs) with broad-spectrum suppression. In this study,
Brevibacillus halotolerans
7WMA2, isolated from marine sediment, produced AMPs against Gram-positive and Gram-negative bacteria. The AMPs were precipitated by ammonium sulfate 30% (w/v) from culture broth and dialyzed by a 1 kDa membrane. Tryptone Soy Agar (TSA) was used for the cultivation and resulted in the largest bacteria-inhibiting zones under aerobic conditions at 25 °C, 48 h. An SDS-PAGE gel overlay test revealed that strain 7WMA2 could produce AMPs of 5–10 kDa and showed no degradation when held at 121 °C for 30 min at a wide pH 2–12 range. The AMPs did not cause toxicity to HeLa cells with concentrations up to 500 µg/mL while increasing the arbitrary unit up to eight times. The study showed that the AMPs produced were unique, with broad-spectrum antimicrobial ability.
Graphical Abstract</description><subject>Aerobic conditions</subject><subject>agar</subject><subject>Ammonium</subject><subject>Ammonium sulfate</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - isolation & purification</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antimicrobial peptides</subject><subject>Antimicrobial Peptides - chemistry</subject><subject>Antimicrobial Peptides - isolation & purification</subject><subject>Antimicrobial Peptides - pharmacology</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biological Techniques</subject><subject>Biotechnology</subject><subject>Brevibacillus</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>culture media</subject><subject>Drug resistance</subject><subject>Drug Resistance, Multiple, Bacterial - drug effects</subject><subject>Gram-negative bacteria</subject><subject>Gram-Negative Bacteria - drug effects</subject><subject>Gram-positive bacteria</subject><subject>Gram-Positive Bacteria - drug effects</subject><subject>HeLa Cells</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Marine sediments</subject><subject>Microbial Sensitivity Tests</subject><subject>Multidrug resistance</subject><subject>Multidrug resistant organisms</subject><subject>multiple drug resistance</subject><subject>Original Paper</subject><subject>Pathogens</subject><subject>Peptides</subject><subject>polyacrylamide gel electrophoresis</subject><subject>Protein Science</subject><subject>Public health</subject><subject>Toxicity</subject><subject>tryptones</subject><issn>1073-6085</issn><issn>1559-0305</issn><issn>1559-0305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1TAQhiMEohd4ARbIEhs2AV_iOF6Go0IrtaJCIJbRJJ6c48rHPthOpfIwPCuGFJBYwMIXjb__t2b-qnrG6CtGqXqdGKdC1JSXRXVb9gfVMZNS11RQ-bDcqRJ1Szt5VJ2kdEMpZ7IRj6sj0dGGK6mOq28XKTjINngC3pDNDiJMGaP9uhbDTHqf7d5OMYwWHLnGQ7YGE1mFaMgcw568iXhrR5isc0siO3AhB4cRfCLq81XPyRwiyTsk_ZTtrc13pN-C9SmTq8UVw7hs6w-YbMrgM7mGvAtb9OlJ9WgGl_Dp_XlafXp79nFzXl--f3ex6S_rSUida2EabNt5lgY0ZRPTRssW6TzJjo9CKsFHajQoBNMYiQxno5URwJjmoNUkTquXq-8hhi8LpjzsbZrQOfAYljSIMjjW6EZ1_0V5p9uOyq7VBX3xF3oTluhLI8WQd02jecsLxVeqjDiliPNwiHYP8W5gdPgR9LAGPZSgh59BD7SInt9bL-MezW_Jr2QLIFYglSe_xfjn73_Yfgc7n7X6</recordid><startdate>202412</startdate><enddate>202412</enddate><creator>Le Han, Ho</creator><creator>Pham, Phu Tran Vinh</creator><creator>Kim, Song-Gun</creator><creator>Chan, Sook Sin</creator><creator>Khoo, Kuan Shiong</creator><creator>Chew, Kit Wayne</creator><creator>Show, Pau Loke</creator><creator>Tran, Thi Ngoc Thu</creator><creator>Nguyen, Hai Thi Viet</creator><creator>Nguyen, Phuong Thi Dong</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-5369-2675</orcidid></search><sort><creationdate>202412</creationdate><title>Isolation and Characterization of Antimicrobial Peptides Isolated from Brevibacillus halotolerans 7WMA2 for the Activity Against Multidrug-Resistant Pathogens</title><author>Le Han, Ho ; Pham, Phu Tran Vinh ; Kim, Song-Gun ; Chan, Sook Sin ; Khoo, Kuan Shiong ; Chew, Kit Wayne ; Show, Pau Loke ; Tran, Thi Ngoc Thu ; Nguyen, Hai Thi Viet ; Nguyen, Phuong Thi Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-3d4e66ff5da901c19d956e0fc582b35732b0d9a7ead4d5e1efd97d3a1192a97c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aerobic conditions</topic><topic>agar</topic><topic>Ammonium</topic><topic>Ammonium sulfate</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - isolation & purification</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antimicrobial peptides</topic><topic>Antimicrobial Peptides - chemistry</topic><topic>Antimicrobial Peptides - isolation & purification</topic><topic>Antimicrobial Peptides - pharmacology</topic><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biological Techniques</topic><topic>Biotechnology</topic><topic>Brevibacillus</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>culture media</topic><topic>Drug resistance</topic><topic>Drug Resistance, Multiple, Bacterial - drug effects</topic><topic>Gram-negative bacteria</topic><topic>Gram-Negative Bacteria - drug effects</topic><topic>Gram-positive bacteria</topic><topic>Gram-Positive Bacteria - drug effects</topic><topic>HeLa Cells</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Marine sediments</topic><topic>Microbial Sensitivity Tests</topic><topic>Multidrug resistance</topic><topic>Multidrug resistant organisms</topic><topic>multiple drug resistance</topic><topic>Original Paper</topic><topic>Pathogens</topic><topic>Peptides</topic><topic>polyacrylamide gel electrophoresis</topic><topic>Protein Science</topic><topic>Public health</topic><topic>Toxicity</topic><topic>tryptones</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Le Han, Ho</creatorcontrib><creatorcontrib>Pham, Phu Tran Vinh</creatorcontrib><creatorcontrib>Kim, Song-Gun</creatorcontrib><creatorcontrib>Chan, Sook Sin</creatorcontrib><creatorcontrib>Khoo, Kuan Shiong</creatorcontrib><creatorcontrib>Chew, Kit Wayne</creatorcontrib><creatorcontrib>Show, Pau Loke</creatorcontrib><creatorcontrib>Tran, Thi Ngoc Thu</creatorcontrib><creatorcontrib>Nguyen, Hai Thi Viet</creatorcontrib><creatorcontrib>Nguyen, Phuong Thi Dong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Le Han, Ho</au><au>Pham, Phu Tran Vinh</au><au>Kim, Song-Gun</au><au>Chan, Sook Sin</au><au>Khoo, Kuan Shiong</au><au>Chew, Kit Wayne</au><au>Show, Pau Loke</au><au>Tran, Thi Ngoc Thu</au><au>Nguyen, Hai Thi Viet</au><au>Nguyen, Phuong Thi Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Characterization of Antimicrobial Peptides Isolated from Brevibacillus halotolerans 7WMA2 for the Activity Against Multidrug-Resistant Pathogens</atitle><jtitle>Molecular biotechnology</jtitle><stitle>Mol Biotechnol</stitle><addtitle>Mol Biotechnol</addtitle><date>2024-12</date><risdate>2024</risdate><volume>66</volume><issue>12</issue><spage>3618</spage><epage>3627</epage><pages>3618-3627</pages><issn>1073-6085</issn><issn>1559-0305</issn><eissn>1559-0305</eissn><abstract>Multidrug resistance to pathogens has posed a severe threat to public health. The threat could be addressed by antimicrobial peptides (AMPs) with broad-spectrum suppression. In this study,
Brevibacillus halotolerans
7WMA2, isolated from marine sediment, produced AMPs against Gram-positive and Gram-negative bacteria. The AMPs were precipitated by ammonium sulfate 30% (w/v) from culture broth and dialyzed by a 1 kDa membrane. Tryptone Soy Agar (TSA) was used for the cultivation and resulted in the largest bacteria-inhibiting zones under aerobic conditions at 25 °C, 48 h. An SDS-PAGE gel overlay test revealed that strain 7WMA2 could produce AMPs of 5–10 kDa and showed no degradation when held at 121 °C for 30 min at a wide pH 2–12 range. The AMPs did not cause toxicity to HeLa cells with concentrations up to 500 µg/mL while increasing the arbitrary unit up to eight times. The study showed that the AMPs produced were unique, with broad-spectrum antimicrobial ability.
Graphical Abstract</abstract><cop>New York</cop><pub>Springer US</pub><pmid>38042757</pmid><doi>10.1007/s12033-023-00963-0</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-5369-2675</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1073-6085 |
| ispartof | Molecular biotechnology, 2024-12, Vol.66 (12), p.3618-3627 |
| issn | 1073-6085 1559-0305 1559-0305 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3154149478 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Aerobic conditions agar Ammonium Ammonium sulfate Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - isolation & purification Anti-Bacterial Agents - pharmacology Antimicrobial peptides Antimicrobial Peptides - chemistry Antimicrobial Peptides - isolation & purification Antimicrobial Peptides - pharmacology Bacteria Biochemistry Biological Techniques Biotechnology Brevibacillus Cell Biology Cell culture Chemistry Chemistry and Materials Science culture media Drug resistance Drug Resistance, Multiple, Bacterial - drug effects Gram-negative bacteria Gram-Negative Bacteria - drug effects Gram-positive bacteria Gram-Positive Bacteria - drug effects HeLa Cells Human Genetics Humans Marine sediments Microbial Sensitivity Tests Multidrug resistance Multidrug resistant organisms multiple drug resistance Original Paper Pathogens Peptides polyacrylamide gel electrophoresis Protein Science Public health Toxicity tryptones |
| title | Isolation and Characterization of Antimicrobial Peptides Isolated from Brevibacillus halotolerans 7WMA2 for the Activity Against Multidrug-Resistant Pathogens |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T17%3A12%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20Characterization%20of%20Antimicrobial%20Peptides%20Isolated%20from%20Brevibacillus%20halotolerans%207WMA2%20for%20the%20Activity%20Against%20Multidrug-Resistant%20Pathogens&rft.jtitle=Molecular%20biotechnology&rft.au=Le%20Han,%20Ho&rft.date=2024-12&rft.volume=66&rft.issue=12&rft.spage=3618&rft.epage=3627&rft.pages=3618-3627&rft.issn=1073-6085&rft.eissn=1559-0305&rft_id=info:doi/10.1007/s12033-023-00963-0&rft_dat=%3Cproquest_cross%3E2896805869%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3128449262&rft_id=info:pmid/38042757&rfr_iscdi=true |