Potential Growth of Anammox Bacteria under Aerobic Conditions
Anammox bacteria are obligate anaerobic bacteria that exist widely in nature with sufficient amounts of dissolved oxygen. However, whether anammox bacteria can grow under aerobic conditions remains unclear. In this study, we found that the production of nitrate in the anammox system under aerobic co...
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| Veröffentlicht in: | Environmental science & technology 2024-10, Vol.58 (41), p.18244-18254 |
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| creator | Sun, Jingqi Feng, Yiming Zheng, Ru Wu, Xiaogang Kong, Lingrui Zhang, Kuo Liu, Sitong |
| description | Anammox bacteria are obligate anaerobic bacteria that exist widely in nature with sufficient amounts of dissolved oxygen. However, whether anammox bacteria can grow under aerobic conditions remains unclear. In this study, we found that the production of nitrate in the anammox system under aerobic conditions was significantly higher than that under anaerobic conditions without total nitrogen loss. Anammox bacteria can grow by oxidizing nitrite and dehydrogenating hydrazine to produce electrons for carbon fixation. The hydrazine dehydrogenase in anammox bacteria was inhibited under aerobic conditions, and the nitrite oxidoreductase transcription expression of anammox bacteria increased by 2.7 times compared to that under anaerobic conditions, which was the main way for anammox bacteria perform carbon fixation. DNA-stable isotope probing with 13C bicarbonate found the existence of anammox bacteria with 13C isotopes in aerobic cultivation, further proving that anammox bacteria can grow under aerobic condition. More than half of the pathways in glycolysis, the Wood-Ljungdahl pathway, and the tricarboxylic acid cycle were upregulated in anammox bacteria in aerobic condition. Large amounts of bacterioferritins are the important antioxidative enzymes in anammox bacteria in the aerobic environment, which contributes to their stronger oxygen adaptation than other anaerobes. This study expands our understanding of the growth mechanism of anammox bacteria as well as the oxygen adaptation strategies of obligate anaerobic bacteria. |
| doi_str_mv | 10.1021/acs.est.4c06413 |
| format | Article |
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However, whether anammox bacteria can grow under aerobic conditions remains unclear. In this study, we found that the production of nitrate in the anammox system under aerobic conditions was significantly higher than that under anaerobic conditions without total nitrogen loss. Anammox bacteria can grow by oxidizing nitrite and dehydrogenating hydrazine to produce electrons for carbon fixation. The hydrazine dehydrogenase in anammox bacteria was inhibited under aerobic conditions, and the nitrite oxidoreductase transcription expression of anammox bacteria increased by 2.7 times compared to that under anaerobic conditions, which was the main way for anammox bacteria perform carbon fixation. DNA-stable isotope probing with 13C bicarbonate found the existence of anammox bacteria with 13C isotopes in aerobic cultivation, further proving that anammox bacteria can grow under aerobic condition. More than half of the pathways in glycolysis, the Wood-Ljungdahl pathway, and the tricarboxylic acid cycle were upregulated in anammox bacteria in aerobic condition. Large amounts of bacterioferritins are the important antioxidative enzymes in anammox bacteria in the aerobic environment, which contributes to their stronger oxygen adaptation than other anaerobes. This study expands our understanding of the growth mechanism of anammox bacteria as well as the oxygen adaptation strategies of obligate anaerobic bacteria.</description><identifier>ISSN: 0013-936X</identifier><identifier>ISSN: 1520-5851</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/acs.est.4c06413</identifier><identifier>PMID: 39352194</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Adaptation ; Aerobic conditions ; Ammonia-oxidizing bacteria ; Anaerobes ; anaerobic ammonium oxidation ; Anaerobic bacteria ; Anaerobic conditions ; Bacteria ; Bicarbonates ; Bioremediation and Biotechnology ; Carbon 13 ; carbon dioxide fixation ; Carbon fixation ; Dehydrogenation ; Dissolved oxygen ; environmental science ; Gene expression ; Glycolysis ; Hydrazine ; Hydrazines ; Isotopes ; nitrates ; nitrites ; Oxidoreductase ; oxidoreductases ; Oxygen ; Planctomycetota ; Stable isotopes ; technology ; total nitrogen ; Tricarboxylic acid cycle</subject><ispartof>Environmental science & technology, 2024-10, Vol.58 (41), p.18244-18254</ispartof><rights>2024 American Chemical Society</rights><rights>Copyright American Chemical Society Oct 15, 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a278t-cf534c7432553c8fc1869ceaf907b7b315618eebcb4a0cbdf79a1aaa0cc07d543</cites><orcidid>0000-0001-6849-8704</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.est.4c06413$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.est.4c06413$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39352194$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Jingqi</creatorcontrib><creatorcontrib>Feng, Yiming</creatorcontrib><creatorcontrib>Zheng, Ru</creatorcontrib><creatorcontrib>Wu, Xiaogang</creatorcontrib><creatorcontrib>Kong, Lingrui</creatorcontrib><creatorcontrib>Zhang, Kuo</creatorcontrib><creatorcontrib>Liu, Sitong</creatorcontrib><title>Potential Growth of Anammox Bacteria under Aerobic Conditions</title><title>Environmental science & technology</title><addtitle>Environ. Sci. Technol</addtitle><description>Anammox bacteria are obligate anaerobic bacteria that exist widely in nature with sufficient amounts of dissolved oxygen. However, whether anammox bacteria can grow under aerobic conditions remains unclear. In this study, we found that the production of nitrate in the anammox system under aerobic conditions was significantly higher than that under anaerobic conditions without total nitrogen loss. Anammox bacteria can grow by oxidizing nitrite and dehydrogenating hydrazine to produce electrons for carbon fixation. The hydrazine dehydrogenase in anammox bacteria was inhibited under aerobic conditions, and the nitrite oxidoreductase transcription expression of anammox bacteria increased by 2.7 times compared to that under anaerobic conditions, which was the main way for anammox bacteria perform carbon fixation. DNA-stable isotope probing with 13C bicarbonate found the existence of anammox bacteria with 13C isotopes in aerobic cultivation, further proving that anammox bacteria can grow under aerobic condition. More than half of the pathways in glycolysis, the Wood-Ljungdahl pathway, and the tricarboxylic acid cycle were upregulated in anammox bacteria in aerobic condition. Large amounts of bacterioferritins are the important antioxidative enzymes in anammox bacteria in the aerobic environment, which contributes to their stronger oxygen adaptation than other anaerobes. This study expands our understanding of the growth mechanism of anammox bacteria as well as the oxygen adaptation strategies of obligate anaerobic bacteria.</description><subject>Adaptation</subject><subject>Aerobic conditions</subject><subject>Ammonia-oxidizing bacteria</subject><subject>Anaerobes</subject><subject>anaerobic ammonium oxidation</subject><subject>Anaerobic bacteria</subject><subject>Anaerobic conditions</subject><subject>Bacteria</subject><subject>Bicarbonates</subject><subject>Bioremediation and Biotechnology</subject><subject>Carbon 13</subject><subject>carbon dioxide fixation</subject><subject>Carbon fixation</subject><subject>Dehydrogenation</subject><subject>Dissolved oxygen</subject><subject>environmental science</subject><subject>Gene expression</subject><subject>Glycolysis</subject><subject>Hydrazine</subject><subject>Hydrazines</subject><subject>Isotopes</subject><subject>nitrates</subject><subject>nitrites</subject><subject>Oxidoreductase</subject><subject>oxidoreductases</subject><subject>Oxygen</subject><subject>Planctomycetota</subject><subject>Stable isotopes</subject><subject>technology</subject><subject>total nitrogen</subject><subject>Tricarboxylic acid cycle</subject><issn>0013-936X</issn><issn>1520-5851</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqNkcFLwzAUxoMobk7P3qTgRZBu7zVJmxw8zKFTGOhBwVtJ0xQ71kaTFvW_N2NzB0Hw9N7h932P732EnCKMERKcKO3HxndjpiFlSPfIEHkCMRcc98kQAGksafoyIEfeLwEgoSAOyYBKyhOUbEiuHm1n2q5Wq2ju7Ef3GtkqmraqaexndK10Z1ytor4tjYumxtmi1tHMtmXd1bb1x-SgUitvTrZzRJ5vb55md_HiYX4_my5ilWSii3XFKdMZownnVItKo0ilNqqSkBVZQZGnKIwpdMEU6KKsMqlQqbBryErO6IhcbHzfnH3vQ-C8qb02q5Vqje19HhyoYBSY_AeKmNJUUBrQ81_o0vauDUEClYBEyXkaqMmG0s5670yVv7m6Ue4rR8jXJeShhHyt3pYQFGdb375oTLnjf74egMsNsFbubv5l9w3_jZDW</recordid><startdate>20241015</startdate><enddate>20241015</enddate><creator>Sun, Jingqi</creator><creator>Feng, Yiming</creator><creator>Zheng, Ru</creator><creator>Wu, Xiaogang</creator><creator>Kong, Lingrui</creator><creator>Zhang, Kuo</creator><creator>Liu, Sitong</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-6849-8704</orcidid></search><sort><creationdate>20241015</creationdate><title>Potential Growth of Anammox Bacteria under Aerobic Conditions</title><author>Sun, Jingqi ; Feng, Yiming ; Zheng, Ru ; Wu, Xiaogang ; Kong, Lingrui ; Zhang, Kuo ; Liu, Sitong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a278t-cf534c7432553c8fc1869ceaf907b7b315618eebcb4a0cbdf79a1aaa0cc07d543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adaptation</topic><topic>Aerobic conditions</topic><topic>Ammonia-oxidizing bacteria</topic><topic>Anaerobes</topic><topic>anaerobic ammonium oxidation</topic><topic>Anaerobic bacteria</topic><topic>Anaerobic conditions</topic><topic>Bacteria</topic><topic>Bicarbonates</topic><topic>Bioremediation and Biotechnology</topic><topic>Carbon 13</topic><topic>carbon dioxide fixation</topic><topic>Carbon fixation</topic><topic>Dehydrogenation</topic><topic>Dissolved oxygen</topic><topic>environmental science</topic><topic>Gene expression</topic><topic>Glycolysis</topic><topic>Hydrazine</topic><topic>Hydrazines</topic><topic>Isotopes</topic><topic>nitrates</topic><topic>nitrites</topic><topic>Oxidoreductase</topic><topic>oxidoreductases</topic><topic>Oxygen</topic><topic>Planctomycetota</topic><topic>Stable isotopes</topic><topic>technology</topic><topic>total nitrogen</topic><topic>Tricarboxylic acid cycle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Jingqi</creatorcontrib><creatorcontrib>Feng, Yiming</creatorcontrib><creatorcontrib>Zheng, Ru</creatorcontrib><creatorcontrib>Wu, Xiaogang</creatorcontrib><creatorcontrib>Kong, Lingrui</creatorcontrib><creatorcontrib>Zhang, Kuo</creatorcontrib><creatorcontrib>Liu, Sitong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Environmental science & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Jingqi</au><au>Feng, Yiming</au><au>Zheng, Ru</au><au>Wu, Xiaogang</au><au>Kong, Lingrui</au><au>Zhang, Kuo</au><au>Liu, Sitong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potential Growth of Anammox Bacteria under Aerobic Conditions</atitle><jtitle>Environmental science & technology</jtitle><addtitle>Environ. Sci. Technol</addtitle><date>2024-10-15</date><risdate>2024</risdate><volume>58</volume><issue>41</issue><spage>18244</spage><epage>18254</epage><pages>18244-18254</pages><issn>0013-936X</issn><issn>1520-5851</issn><eissn>1520-5851</eissn><abstract>Anammox bacteria are obligate anaerobic bacteria that exist widely in nature with sufficient amounts of dissolved oxygen. However, whether anammox bacteria can grow under aerobic conditions remains unclear. In this study, we found that the production of nitrate in the anammox system under aerobic conditions was significantly higher than that under anaerobic conditions without total nitrogen loss. Anammox bacteria can grow by oxidizing nitrite and dehydrogenating hydrazine to produce electrons for carbon fixation. The hydrazine dehydrogenase in anammox bacteria was inhibited under aerobic conditions, and the nitrite oxidoreductase transcription expression of anammox bacteria increased by 2.7 times compared to that under anaerobic conditions, which was the main way for anammox bacteria perform carbon fixation. DNA-stable isotope probing with 13C bicarbonate found the existence of anammox bacteria with 13C isotopes in aerobic cultivation, further proving that anammox bacteria can grow under aerobic condition. More than half of the pathways in glycolysis, the Wood-Ljungdahl pathway, and the tricarboxylic acid cycle were upregulated in anammox bacteria in aerobic condition. Large amounts of bacterioferritins are the important antioxidative enzymes in anammox bacteria in the aerobic environment, which contributes to their stronger oxygen adaptation than other anaerobes. This study expands our understanding of the growth mechanism of anammox bacteria as well as the oxygen adaptation strategies of obligate anaerobic bacteria.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>39352194</pmid><doi>10.1021/acs.est.4c06413</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-6849-8704</orcidid></addata></record> |
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| subjects | Adaptation Aerobic conditions Ammonia-oxidizing bacteria Anaerobes anaerobic ammonium oxidation Anaerobic bacteria Anaerobic conditions Bacteria Bicarbonates Bioremediation and Biotechnology Carbon 13 carbon dioxide fixation Carbon fixation Dehydrogenation Dissolved oxygen environmental science Gene expression Glycolysis Hydrazine Hydrazines Isotopes nitrates nitrites Oxidoreductase oxidoreductases Oxygen Planctomycetota Stable isotopes technology total nitrogen Tricarboxylic acid cycle |
| title | Potential Growth of Anammox Bacteria under Aerobic Conditions |
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