Molecular detection of Leishmania and blood meal analysis in sand flies from Corumbá, Mato Grosso do Sul, Brazil
•Detection of Leishmania braziliensis DNA in Lutzomyia cruzi.•Identification of three food sources in engorged females of sand flies.•Potential role of Lutzomia cruzi in transmitting Leishmania braziliensis. In this study, we investigated the presence of Leishmania in sand flies collected from a per...
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| Veröffentlicht in: | Acta tropica 2023-09, Vol.245, p.106961-106961, Article 106961 |
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| creator | Dutra-Rêgo, Felipe Lima, Mariana Alves Almeida, Giovana Luísa Pereira de Almeida, Paulo Silva Bastos, Grace Kelly Sguario do Valle Alexandre, Luiza Vilalva das Neves Samaniego, Rodrigo Daltro da Silva, Walkiria Arruda Ogaya, Alcides de Moraes Andrade-Filho, José Dilermando |
| description | •Detection of Leishmania braziliensis DNA in Lutzomyia cruzi.•Identification of three food sources in engorged females of sand flies.•Potential role of Lutzomia cruzi in transmitting Leishmania braziliensis.
In this study, we investigated the presence of Leishmania in sand flies collected from a peridomestic area in Corumbá, Mato Grosso do Sul, after an autochthonous case of cutaneous leishmaniasis was confirmed. A total of 1,542 sand flies belonging to seven species were collected, with Lu. cruzi being the most prevalent (94.3%). We detected the presence of DNA from Le. infantum (7 pools) and Le. braziliensis (3 pools) by sequencing the ITS1 amplicon in ten pools, all of which were composed of engorged (3) and non-engorged (7) females of Lu. cruzi. We collected 24 engorged females, with Homo sapiens being the most common blood meal source (91.6%), followed by Dasyprocta azarae and Canis lupus familiaris (4.2% each). To our knowledge, this is the first molecular evidence of Le. braziliensis in wild-caught Lu. cruzi in Brazil, suggesting its potential role as a vector for this parasite. |
| doi_str_mv | 10.1016/j.actatropica.2023.106961 |
| format | Article |
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In this study, we investigated the presence of Leishmania in sand flies collected from a peridomestic area in Corumbá, Mato Grosso do Sul, after an autochthonous case of cutaneous leishmaniasis was confirmed. A total of 1,542 sand flies belonging to seven species were collected, with Lu. cruzi being the most prevalent (94.3%). We detected the presence of DNA from Le. infantum (7 pools) and Le. braziliensis (3 pools) by sequencing the ITS1 amplicon in ten pools, all of which were composed of engorged (3) and non-engorged (7) females of Lu. cruzi. We collected 24 engorged females, with Homo sapiens being the most common blood meal source (91.6%), followed by Dasyprocta azarae and Canis lupus familiaris (4.2% each). To our knowledge, this is the first molecular evidence of Le. braziliensis in wild-caught Lu. cruzi in Brazil, suggesting its potential role as a vector for this parasite.</description><identifier>ISSN: 0001-706X</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/j.actatropica.2023.106961</identifier><identifier>PMID: 37268196</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>blood meal ; Brazil ; Canis lupus ; Cutaneous leishmaniasis ; Dasyprocta ; DNA ; Leishmania ; Leishmania infantum ; Lutzomyia ; Molecular detection ; parasites ; sand ; Sand fly ; sibling species ; species</subject><ispartof>Acta tropica, 2023-09, Vol.245, p.106961-106961, Article 106961</ispartof><rights>2023</rights><rights>Copyright © 2023. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c269t-1e5794476c489d508bab18de5138d80646acb03dfb9ff9f190bd0886719974093</cites><orcidid>0000-0002-7418-8208 ; 0000-0002-2799-8267</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0001706X23001481$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37268196$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dutra-Rêgo, Felipe</creatorcontrib><creatorcontrib>Lima, Mariana Alves</creatorcontrib><creatorcontrib>Almeida, Giovana Luísa Pereira</creatorcontrib><creatorcontrib>de Almeida, Paulo Silva</creatorcontrib><creatorcontrib>Bastos, Grace Kelly Sguario do Valle</creatorcontrib><creatorcontrib>Alexandre, Luiza Vilalva das Neves</creatorcontrib><creatorcontrib>Samaniego, Rodrigo Daltro</creatorcontrib><creatorcontrib>da Silva, Walkiria Arruda</creatorcontrib><creatorcontrib>Ogaya, Alcides de Moraes</creatorcontrib><creatorcontrib>Andrade-Filho, José Dilermando</creatorcontrib><title>Molecular detection of Leishmania and blood meal analysis in sand flies from Corumbá, Mato Grosso do Sul, Brazil</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>•Detection of Leishmania braziliensis DNA in Lutzomyia cruzi.•Identification of three food sources in engorged females of sand flies.•Potential role of Lutzomia cruzi in transmitting Leishmania braziliensis.
In this study, we investigated the presence of Leishmania in sand flies collected from a peridomestic area in Corumbá, Mato Grosso do Sul, after an autochthonous case of cutaneous leishmaniasis was confirmed. A total of 1,542 sand flies belonging to seven species were collected, with Lu. cruzi being the most prevalent (94.3%). We detected the presence of DNA from Le. infantum (7 pools) and Le. braziliensis (3 pools) by sequencing the ITS1 amplicon in ten pools, all of which were composed of engorged (3) and non-engorged (7) females of Lu. cruzi. We collected 24 engorged females, with Homo sapiens being the most common blood meal source (91.6%), followed by Dasyprocta azarae and Canis lupus familiaris (4.2% each). To our knowledge, this is the first molecular evidence of Le. braziliensis in wild-caught Lu. cruzi in Brazil, suggesting its potential role as a vector for this parasite.</description><subject>blood meal</subject><subject>Brazil</subject><subject>Canis lupus</subject><subject>Cutaneous leishmaniasis</subject><subject>Dasyprocta</subject><subject>DNA</subject><subject>Leishmania</subject><subject>Leishmania infantum</subject><subject>Lutzomyia</subject><subject>Molecular detection</subject><subject>parasites</subject><subject>sand</subject><subject>Sand fly</subject><subject>sibling species</subject><subject>species</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqNkc1u1DAQgC0EotvCKyBz49Bs_ZP45wgraJG24gBI3CzHHguvnHhrJ5XK2_AsfTGy2oK4tafRjL-ZkedD6C0la0qouNitrZvsVPI-OrtmhPGlLrSgz9CKKskbwbr2OVoRQmgjifhxgk5r3S0Zkx17iU64ZEJRLVbo5joncHOyBXuYwE0xjzgHvIVYfw52jBbb0eM-5ezxADYtqU13NVYcR1wPbyFFqDiUPOBNLvPQ3_8-x9d2yviy5Foz9hl_ndM5_lDsr5heoRfBpgqvH-IZ-v7p47fNVbP9cvl5837bOCb01FDopG5bKVyrtO-I6m1PlYeOcuUVEa2wrifch16HoAPVpPdEKSGp1rIlmp-hd8e5-5JvZqiTGWJ1kJIdIc_VcNpxxSnR9FGUKca41LrtFlQfUXf4W4Fg9iUOttwZSsxBjtmZ_-SYgxxzlLP0vnlYM_cD-H-df20swOYIwHKX2wjFVBdhdOBjWdQYn-MT1vwBJ_WmGg</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Dutra-Rêgo, Felipe</creator><creator>Lima, Mariana Alves</creator><creator>Almeida, Giovana Luísa Pereira</creator><creator>de Almeida, Paulo Silva</creator><creator>Bastos, Grace Kelly Sguario do Valle</creator><creator>Alexandre, Luiza Vilalva das Neves</creator><creator>Samaniego, Rodrigo Daltro</creator><creator>da Silva, Walkiria Arruda</creator><creator>Ogaya, Alcides de Moraes</creator><creator>Andrade-Filho, José Dilermando</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-7418-8208</orcidid><orcidid>https://orcid.org/0000-0002-2799-8267</orcidid></search><sort><creationdate>20230901</creationdate><title>Molecular detection of Leishmania and blood meal analysis in sand flies from Corumbá, Mato Grosso do Sul, Brazil</title><author>Dutra-Rêgo, Felipe ; Lima, Mariana Alves ; Almeida, Giovana Luísa Pereira ; de Almeida, Paulo Silva ; Bastos, Grace Kelly Sguario do Valle ; Alexandre, Luiza Vilalva das Neves ; Samaniego, Rodrigo Daltro ; da Silva, Walkiria Arruda ; Ogaya, Alcides de Moraes ; Andrade-Filho, José Dilermando</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c269t-1e5794476c489d508bab18de5138d80646acb03dfb9ff9f190bd0886719974093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>blood meal</topic><topic>Brazil</topic><topic>Canis lupus</topic><topic>Cutaneous leishmaniasis</topic><topic>Dasyprocta</topic><topic>DNA</topic><topic>Leishmania</topic><topic>Leishmania infantum</topic><topic>Lutzomyia</topic><topic>Molecular detection</topic><topic>parasites</topic><topic>sand</topic><topic>Sand fly</topic><topic>sibling species</topic><topic>species</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dutra-Rêgo, Felipe</creatorcontrib><creatorcontrib>Lima, Mariana Alves</creatorcontrib><creatorcontrib>Almeida, Giovana Luísa Pereira</creatorcontrib><creatorcontrib>de Almeida, Paulo Silva</creatorcontrib><creatorcontrib>Bastos, Grace Kelly Sguario do Valle</creatorcontrib><creatorcontrib>Alexandre, Luiza Vilalva das Neves</creatorcontrib><creatorcontrib>Samaniego, Rodrigo Daltro</creatorcontrib><creatorcontrib>da Silva, Walkiria Arruda</creatorcontrib><creatorcontrib>Ogaya, Alcides de Moraes</creatorcontrib><creatorcontrib>Andrade-Filho, José Dilermando</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dutra-Rêgo, Felipe</au><au>Lima, Mariana Alves</au><au>Almeida, Giovana Luísa Pereira</au><au>de Almeida, Paulo Silva</au><au>Bastos, Grace Kelly Sguario do Valle</au><au>Alexandre, Luiza Vilalva das Neves</au><au>Samaniego, Rodrigo Daltro</au><au>da Silva, Walkiria Arruda</au><au>Ogaya, Alcides de Moraes</au><au>Andrade-Filho, José Dilermando</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular detection of Leishmania and blood meal analysis in sand flies from Corumbá, Mato Grosso do Sul, Brazil</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>2023-09-01</date><risdate>2023</risdate><volume>245</volume><spage>106961</spage><epage>106961</epage><pages>106961-106961</pages><artnum>106961</artnum><issn>0001-706X</issn><eissn>1873-6254</eissn><abstract>•Detection of Leishmania braziliensis DNA in Lutzomyia cruzi.•Identification of three food sources in engorged females of sand flies.•Potential role of Lutzomia cruzi in transmitting Leishmania braziliensis.
In this study, we investigated the presence of Leishmania in sand flies collected from a peridomestic area in Corumbá, Mato Grosso do Sul, after an autochthonous case of cutaneous leishmaniasis was confirmed. A total of 1,542 sand flies belonging to seven species were collected, with Lu. cruzi being the most prevalent (94.3%). We detected the presence of DNA from Le. infantum (7 pools) and Le. braziliensis (3 pools) by sequencing the ITS1 amplicon in ten pools, all of which were composed of engorged (3) and non-engorged (7) females of Lu. cruzi. We collected 24 engorged females, with Homo sapiens being the most common blood meal source (91.6%), followed by Dasyprocta azarae and Canis lupus familiaris (4.2% each). To our knowledge, this is the first molecular evidence of Le. braziliensis in wild-caught Lu. cruzi in Brazil, suggesting its potential role as a vector for this parasite.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>37268196</pmid><doi>10.1016/j.actatropica.2023.106961</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-7418-8208</orcidid><orcidid>https://orcid.org/0000-0002-2799-8267</orcidid></addata></record> |
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| subjects | blood meal Brazil Canis lupus Cutaneous leishmaniasis Dasyprocta DNA Leishmania Leishmania infantum Lutzomyia Molecular detection parasites sand Sand fly sibling species species |
| title | Molecular detection of Leishmania and blood meal analysis in sand flies from Corumbá, Mato Grosso do Sul, Brazil |
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