A new hormonal protocol supports early development of in vitro‐produced embryos after transfer to anoestrus mares
The present study aimed to evaluate whether primed anoestrus mares are suitable recipients for embryos produced by intracytoplasmic sperm injection (ICSI). Anoestrus was confirmed in four mares and daily doses of oestradiol benzoate (6 mg in total) over 5 days were administered; after 3 days of rest...
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| Veröffentlicht in: | Reproduction in domestic animals 2024-10, Vol.59 (S3), p.e14622-n/a |
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| container_title | Reproduction in domestic animals |
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| creator | Muñoz‐García, Carmen C. Luis‐Calero, Marcos Fernández‐Hernández, Pablo Pizarro‐Hernández, Alba Fernández‐González, Raúl Gutiérrez‐Adán, Alfonso Sánchez‐Calabuig, María Jesús Cuervo‐Arango, Juan González‐Fernández, Lauro Macías‐García, Beatriz |
| description | The present study aimed to evaluate whether primed anoestrus mares are suitable recipients for embryos produced by intracytoplasmic sperm injection (ICSI). Anoestrus was confirmed in four mares and daily doses of oestradiol benzoate (6 mg in total) over 5 days were administered; after 3 days of rest, oral altrenogest was administered at 0.088 mg/kg and embryos (1 to 5 embryos per mare; 15 in total) were transferred 3.5 days after progesterone onset. Uterine lavage was conducted 48 h after transfer. The results revealed an 80% embryo recovery rate, and among the retrieved embryos, 67% showed evident intrauterine development. Hence, ICSI‐derived embryos can be successfully transferred to primed anoestrus mares, but more studies are required to ensure further embryo development and foaling. |
| doi_str_mv | 10.1111/rda.14622 |
| format | Article |
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Anoestrus was confirmed in four mares and daily doses of oestradiol benzoate (6 mg in total) over 5 days were administered; after 3 days of rest, oral altrenogest was administered at 0.088 mg/kg and embryos (1 to 5 embryos per mare; 15 in total) were transferred 3.5 days after progesterone onset. Uterine lavage was conducted 48 h after transfer. The results revealed an 80% embryo recovery rate, and among the retrieved embryos, 67% showed evident intrauterine development. Hence, ICSI‐derived embryos can be successfully transferred to primed anoestrus mares, but more studies are required to ensure further embryo development and foaling.</description><identifier>ISSN: 0936-6768</identifier><identifier>ISSN: 1439-0531</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.14622</identifier><identifier>PMID: 39396857</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>anestrus ; Anestrus - drug effects ; Animals ; anoestrus recipient ; Benzoates ; Benzoic acid ; early development ; Embryo Transfer - veterinary ; embryogenesis ; Embryonic Development - drug effects ; Embryos ; estradiol ; Estradiol - administration & dosage ; Estradiol - analogs & derivatives ; Estradiol - pharmacology ; Female ; Horses - embryology ; in vitro embryo ; intracytoplasmic sperm injection ; mare ; mares ; Pregnancy ; Progesterone ; Progesterone - pharmacology ; Sperm Injections, Intracytoplasmic - veterinary ; transfer ; Trenbolone Acetate - administration & dosage ; Trenbolone Acetate - analogs & derivatives ; Trenbolone Acetate - pharmacology</subject><ispartof>Reproduction in domestic animals, 2024-10, Vol.59 (S3), p.e14622-n/a</ispartof><rights>2024 The Author(s). published by Wiley‐VCH GmbH.</rights><rights>2024 The Author(s). Reproduction in Domestic Animals published by Wiley‐VCH GmbH.</rights><rights>2024. This article is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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Anoestrus was confirmed in four mares and daily doses of oestradiol benzoate (6 mg in total) over 5 days were administered; after 3 days of rest, oral altrenogest was administered at 0.088 mg/kg and embryos (1 to 5 embryos per mare; 15 in total) were transferred 3.5 days after progesterone onset. Uterine lavage was conducted 48 h after transfer. The results revealed an 80% embryo recovery rate, and among the retrieved embryos, 67% showed evident intrauterine development. Hence, ICSI‐derived embryos can be successfully transferred to primed anoestrus mares, but more studies are required to ensure further embryo development and foaling.</description><subject>anestrus</subject><subject>Anestrus - drug effects</subject><subject>Animals</subject><subject>anoestrus recipient</subject><subject>Benzoates</subject><subject>Benzoic acid</subject><subject>early development</subject><subject>Embryo Transfer - veterinary</subject><subject>embryogenesis</subject><subject>Embryonic Development - drug effects</subject><subject>Embryos</subject><subject>estradiol</subject><subject>Estradiol - administration & dosage</subject><subject>Estradiol - analogs & derivatives</subject><subject>Estradiol - pharmacology</subject><subject>Female</subject><subject>Horses - embryology</subject><subject>in vitro embryo</subject><subject>intracytoplasmic sperm injection</subject><subject>mare</subject><subject>mares</subject><subject>Pregnancy</subject><subject>Progesterone</subject><subject>Progesterone - pharmacology</subject><subject>Sperm Injections, Intracytoplasmic - veterinary</subject><subject>transfer</subject><subject>Trenbolone Acetate - administration & dosage</subject><subject>Trenbolone Acetate - analogs & derivatives</subject><subject>Trenbolone Acetate - pharmacology</subject><issn>0936-6768</issn><issn>1439-0531</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNqNkc1q3DAQx0Vp6G7THvoCQdBLe_CuPmzZOi75amEhEJKzkaUR9WJbjmRv2FseoY-QZ8mj9Emi1JscAoEIBs3Ab_7MzB-hb5QsaHxLb9SCpoKxD2hOUy4TknH6Ec2J5CIRuShm6HMIG0JoVuT5JzTjkktRZPkcDSvcwS3-43zrOtXg3rvBadfgMPa980PAoHyzwwa20Li-hW7AzuK6e7jf1oN3_-7-xhYzajAY2srvXMDKDuDx4FUX7FPisOochMGPAbfKQ_iCDqxqAnzd_4fo-uz06vhXsr44_328Wiea5YIlAgqAlADVlSU5F1KkmkklCmaISY2NRW6trKjkqbQGbKUzrVOtVSFAAvBD9GPSjSPejHGCsq2DhqZRHbgxlJxmvGBC8uIdKBWci5zRiH5_hW7c6OPxJirlMVikfk6U9i4ED7bsfR2335WUlE-uldG18r9rkT3aK45VC-aFfLYpAssJuK0b2L2tVF6erCbJR6iUpSg</recordid><startdate>202410</startdate><enddate>202410</enddate><creator>Muñoz‐García, Carmen C.</creator><creator>Luis‐Calero, Marcos</creator><creator>Fernández‐Hernández, Pablo</creator><creator>Pizarro‐Hernández, Alba</creator><creator>Fernández‐González, Raúl</creator><creator>Gutiérrez‐Adán, Alfonso</creator><creator>Sánchez‐Calabuig, María Jesús</creator><creator>Cuervo‐Arango, Juan</creator><creator>González‐Fernández, Lauro</creator><creator>Macías‐García, Beatriz</creator><general>Blackwell Publishing Ltd</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-4280-992X</orcidid><orcidid>https://orcid.org/0000-0001-5445-1630</orcidid><orcidid>https://orcid.org/0000-0001-5327-4230</orcidid><orcidid>https://orcid.org/0000-0001-5331-9793</orcidid><orcidid>https://orcid.org/0009-0009-6733-4834</orcidid><orcidid>https://orcid.org/0000-0001-5568-548X</orcidid></search><sort><creationdate>202410</creationdate><title>A new hormonal protocol supports early development of in vitro‐produced embryos after transfer to anoestrus mares</title><author>Muñoz‐García, Carmen C. ; 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| fulltext | fulltext |
| identifier | ISSN: 0936-6768 |
| ispartof | Reproduction in domestic animals, 2024-10, Vol.59 (S3), p.e14622-n/a |
| issn | 0936-6768 1439-0531 1439-0531 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | anestrus Anestrus - drug effects Animals anoestrus recipient Benzoates Benzoic acid early development Embryo Transfer - veterinary embryogenesis Embryonic Development - drug effects Embryos estradiol Estradiol - administration & dosage Estradiol - analogs & derivatives Estradiol - pharmacology Female Horses - embryology in vitro embryo intracytoplasmic sperm injection mare mares Pregnancy Progesterone Progesterone - pharmacology Sperm Injections, Intracytoplasmic - veterinary transfer Trenbolone Acetate - administration & dosage Trenbolone Acetate - analogs & derivatives Trenbolone Acetate - pharmacology |
| title | A new hormonal protocol supports early development of in vitro‐produced embryos after transfer to anoestrus mares |
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