Elucidation of a specific alginate lyase Aly7Rm: The products demonstrated the strict recognition of G residue at subsites ±2

An alginate lyase Aly7Rm with novel subsite specificity was cloned, expressed and characterized in this article. Aly7Rm was a G-specific alginate lyase, exhibiting optimum reaction conditions at 30 °C and pH 5.0. UPSEC-VWD-MS analysis revealed that Aly7Rm degraded polysaccharides in a random endo-ac...

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Veröffentlicht in:	Food bioscience 2024-12, Vol.62, p.105196, Article 105196
Hauptverfasser:	Li, Jiajing, Liu, Guanchen, Song, Xiao, Zhang, Yuying, Zhou, Jinhang, Chang, Yaoguang, Xue, Changhu
Format:	Artikel
Sprache:	eng
Schlagworte:	Action pattern Alginate lyase alginates Oligosaccharide products Subsite specificity trisaccharides
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