Methanolic and hydroalcoholic extract of stevia stems have antihyperglycemic and antilipid activity
The use of Stevia rebaudiana stems leaves can negatively interfere with the process of obtaining and purifying sweeteners. If separated, they account for 30 % of the plant's biomass, besides being used as a supplement to animal feed. This pioneering study performed commonly used extractions fro...
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| Veröffentlicht in: | Food bioscience 2024-04, Vol.58, p.103690, Article 103690 |
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| creator | Nogueira, Natani Caroline Cabeça, Cynthia Letícia Serra Siciliano, Paulo Leonardo Marotti Pereira, Betânea Campangolli Zorzenon, Maria Rosa Trentin Dacome, Antonio Sergio Souza, Felipe de Oliveira Pilau, Eduardo Jorge Enokida, Milena Kazue Oliveira, Adan Rodrigues de Silva, Pauline Godoi Costa, Silvio Claudio da Fernandes, Paula Gimenez Milani |
| description | The use of Stevia rebaudiana stems leaves can negatively interfere with the process of obtaining and purifying sweeteners. If separated, they account for 30 % of the plant's biomass, besides being used as a supplement to animal feed. This pioneering study performed commonly used extractions from the leaves. We characterized the extracts (methanolic and hydroalcoholic) and their fractions (ethyl acetate) by UHPLC-MS/MS. Besides, we evaluated their antioxidant, antidiabetic, and antiobesity capacity in vitro. The extracts had 17 % of phenolics, with antioxidant activity of about 65 %. The fraction with 26 % of phenolics showed an antioxidant capacity of 89 %. The lipase inhibition rate was higher than the fraction, about 2.5 times. The same happened with the enzymes α-amylase and α-glucosidase, where the hydroalcoholic extract showed 30 and 90 % inhibition, respectively. These results show that the stem is a valuable co-product for the Stevia production chain. |
| doi_str_mv | 10.1016/j.fbio.2024.103690 |
| format | Article |
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If separated, they account for 30 % of the plant's biomass, besides being used as a supplement to animal feed. This pioneering study performed commonly used extractions from the leaves. We characterized the extracts (methanolic and hydroalcoholic) and their fractions (ethyl acetate) by UHPLC-MS/MS. Besides, we evaluated their antioxidant, antidiabetic, and antiobesity capacity in vitro. The extracts had 17 % of phenolics, with antioxidant activity of about 65 %. The fraction with 26 % of phenolics showed an antioxidant capacity of 89 %. The lipase inhibition rate was higher than the fraction, about 2.5 times. The same happened with the enzymes α-amylase and α-glucosidase, where the hydroalcoholic extract showed 30 and 90 % inhibition, respectively. 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Cabeça, Cynthia Letícia Serra ; Siciliano, Paulo Leonardo Marotti ; Pereira, Betânea Campangolli ; Zorzenon, Maria Rosa Trentin ; Dacome, Antonio Sergio ; Souza, Felipe de Oliveira ; Pilau, Eduardo Jorge ; Enokida, Milena Kazue ; Oliveira, Adan Rodrigues de ; Silva, Pauline Godoi ; Costa, Silvio Claudio da ; Fernandes, Paula Gimenez Milani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c284t-8c5197e6852d68689dc28caccce0819af464012c8781f0c1a5872754cbf97ff13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>alpha-amylase</topic><topic>antioxidant activity</topic><topic>antioxidants</topic><topic>biomass</topic><topic>carboxylic ester hydrolases</topic><topic>coproducts</topic><topic>ethyl acetate</topic><topic>feeds</topic><topic>glycemic effect</topic><topic>Lipase</topic><topic>Phenolic compounds</topic><topic>Stem ethyl acetate fraction</topic><topic>Stevia rebaudiana</topic><topic>α-amylase</topic><topic>α-glucosidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nogueira, Natani Caroline</creatorcontrib><creatorcontrib>Cabeça, Cynthia Letícia Serra</creatorcontrib><creatorcontrib>Siciliano, Paulo Leonardo Marotti</creatorcontrib><creatorcontrib>Pereira, Betânea Campangolli</creatorcontrib><creatorcontrib>Zorzenon, Maria Rosa Trentin</creatorcontrib><creatorcontrib>Dacome, Antonio Sergio</creatorcontrib><creatorcontrib>Souza, Felipe de Oliveira</creatorcontrib><creatorcontrib>Pilau, Eduardo Jorge</creatorcontrib><creatorcontrib>Enokida, Milena Kazue</creatorcontrib><creatorcontrib>Oliveira, Adan Rodrigues de</creatorcontrib><creatorcontrib>Silva, Pauline Godoi</creatorcontrib><creatorcontrib>Costa, Silvio Claudio da</creatorcontrib><creatorcontrib>Fernandes, Paula Gimenez Milani</creatorcontrib><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Food bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nogueira, Natani Caroline</au><au>Cabeça, Cynthia Letícia Serra</au><au>Siciliano, Paulo Leonardo Marotti</au><au>Pereira, Betânea Campangolli</au><au>Zorzenon, Maria Rosa Trentin</au><au>Dacome, Antonio Sergio</au><au>Souza, Felipe de Oliveira</au><au>Pilau, Eduardo Jorge</au><au>Enokida, Milena Kazue</au><au>Oliveira, Adan Rodrigues de</au><au>Silva, Pauline Godoi</au><au>Costa, Silvio Claudio da</au><au>Fernandes, Paula Gimenez Milani</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methanolic and hydroalcoholic extract of stevia stems have antihyperglycemic and antilipid activity</atitle><jtitle>Food bioscience</jtitle><date>2024-04</date><risdate>2024</risdate><volume>58</volume><spage>103690</spage><pages>103690-</pages><artnum>103690</artnum><issn>2212-4292</issn><eissn>2212-4306</eissn><abstract>The use of Stevia rebaudiana stems leaves can negatively interfere with the process of obtaining and purifying sweeteners. If separated, they account for 30 % of the plant's biomass, besides being used as a supplement to animal feed. This pioneering study performed commonly used extractions from the leaves. We characterized the extracts (methanolic and hydroalcoholic) and their fractions (ethyl acetate) by UHPLC-MS/MS. Besides, we evaluated their antioxidant, antidiabetic, and antiobesity capacity in vitro. The extracts had 17 % of phenolics, with antioxidant activity of about 65 %. The fraction with 26 % of phenolics showed an antioxidant capacity of 89 %. The lipase inhibition rate was higher than the fraction, about 2.5 times. The same happened with the enzymes α-amylase and α-glucosidase, where the hydroalcoholic extract showed 30 and 90 % inhibition, respectively. 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| ispartof | Food bioscience, 2024-04, Vol.58, p.103690, Article 103690 |
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| language | eng |
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| subjects | alpha-amylase antioxidant activity antioxidants biomass carboxylic ester hydrolases coproducts ethyl acetate feeds glycemic effect Lipase Phenolic compounds Stem ethyl acetate fraction Stevia rebaudiana α-amylase α-glucosidase |
| title | Methanolic and hydroalcoholic extract of stevia stems have antihyperglycemic and antilipid activity |
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