Growth hormone stimulates the in vitro development and establishment of Haemonchus contortus in sheep
The physiologic increase in some sex hormones has been associated with an increase in the parasite load caused by Haemonchus contortus in ewes, especially prolactin. In lambs that are especially susceptible to hemonchosis, the levels of sex hormones are low; in contrast, the levels of another pituit...
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| Veröffentlicht in: | Veterinary parasitology 2024-06, Vol.328, p.110166, Article 110166 |
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| creator | Sánchez-Paredes, Adolfo Cuenca-Verde, César Prado-Ochoa, María Guadalupe Morales-Montor, Jorge Alba-Hurtado, Fernando Muñoz-Guzmán, Marco Antonio |
| description | The physiologic increase in some sex hormones has been associated with an increase in the parasite load caused by Haemonchus contortus in ewes, especially prolactin. In lambs that are especially susceptible to hemonchosis, the levels of sex hormones are low; in contrast, the levels of another pituitary hormone, growth hormone (GH), which is structurally very similar to prolactin, are high. In this study, the in vitro and in vivo effects of GH on H. contortus larvae development and establishment were evaluated. The addition of 20 ng/mL GH for 5 and 10 days to cultures of H. contortus larvae induced an enlargement (p |
| doi_str_mv | 10.1016/j.vetpar.2024.110166 |
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•In vitro and in vivo effects of growth hormone (GH) on H. contortus were evaluated.•In vitro exposure to GH increased elargement and molting in H. contortus larvae.•3.8% of cells of larvae of H. contortus showed like-receptors for GH.•GH increased parasite load and antibodies levels in lambs infected with H.contortus.</description><identifier>ISSN: 0304-4017</identifier><identifier>ISSN: 1873-2550</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2024.110166</identifier><identifier>PMID: 38508098</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>abomasum ; adults ; Animals ; confocal microscopy ; Experimental infection ; Female ; females ; flow cytometry ; fluorescent antibody technique ; Growth hormone ; Growth Hormone - pharmacology ; Haemonchiasis - parasitology ; Haemonchiasis - veterinary ; Haemonchus - drug effects ; Haemonchus contortus ; Hormonal receptor ; intestines ; Larva - drug effects ; Larva - growth & development ; Male ; parasite load ; parasites ; prolactin ; Receptors, Somatotropin - metabolism ; Sheep ; Sheep Diseases - parasitology ; somatotropin ; veterinary parasitology</subject><ispartof>Veterinary parasitology, 2024-06, Vol.328, p.110166, Article 110166</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c377t-e4d3c181ce76a1d0003c6117d15e3082af321c6d51b83e8ae7738c2c769273e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304401724000542$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38508098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sánchez-Paredes, Adolfo</creatorcontrib><creatorcontrib>Cuenca-Verde, César</creatorcontrib><creatorcontrib>Prado-Ochoa, María Guadalupe</creatorcontrib><creatorcontrib>Morales-Montor, Jorge</creatorcontrib><creatorcontrib>Alba-Hurtado, Fernando</creatorcontrib><creatorcontrib>Muñoz-Guzmán, Marco Antonio</creatorcontrib><title>Growth hormone stimulates the in vitro development and establishment of Haemonchus contortus in sheep</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>The physiologic increase in some sex hormones has been associated with an increase in the parasite load caused by Haemonchus contortus in ewes, especially prolactin. In lambs that are especially susceptible to hemonchosis, the levels of sex hormones are low; in contrast, the levels of another pituitary hormone, growth hormone (GH), which is structurally very similar to prolactin, are high. In this study, the in vitro and in vivo effects of GH on H. contortus larvae development and establishment were evaluated. The addition of 20 ng/mL GH for 5 and 10 days to cultures of H. contortus larvae induced an enlargement (p<0.01) and an L3/L4 molting rate (p<0.03) greater than that of untreated larvae or those treated with other concentrations of the hormone. Flow cytometry showed that 3.8% of the largest and most complex cells of newly obtained larvae of H. contortus were positive for the GH receptor, and by immunofluorescence with confocal microscopy, it was observed that these receptors are located in the intestinal region larvae. In the in vivo assay, the administration of recombinant GH to gonadectomized lambs produced an increase in FEC (p<0.03), the number of female adult worms in the abomasum (p<0.05) and the levels of specific antibodies (p<0.04) in relation to the control lambs; however, it did not affect the fertility of H. contortus females. Although many factors affect the development and implantation of H. contortus in the abomasum of sheep, the results of this study strongly suggest that GH participates in the development and establishment of the parasite in sheep, mainly in young sheep.
[Display omitted]
•In vitro and in vivo effects of growth hormone (GH) on H. contortus were evaluated.•In vitro exposure to GH increased elargement and molting in H. contortus larvae.•3.8% of cells of larvae of H. contortus showed like-receptors for GH.•GH increased parasite load and antibodies levels in lambs infected with H.contortus.</description><subject>abomasum</subject><subject>adults</subject><subject>Animals</subject><subject>confocal microscopy</subject><subject>Experimental infection</subject><subject>Female</subject><subject>females</subject><subject>flow cytometry</subject><subject>fluorescent antibody technique</subject><subject>Growth hormone</subject><subject>Growth Hormone - pharmacology</subject><subject>Haemonchiasis - parasitology</subject><subject>Haemonchiasis - veterinary</subject><subject>Haemonchus - drug effects</subject><subject>Haemonchus contortus</subject><subject>Hormonal receptor</subject><subject>intestines</subject><subject>Larva - drug effects</subject><subject>Larva - growth & development</subject><subject>Male</subject><subject>parasite load</subject><subject>parasites</subject><subject>prolactin</subject><subject>Receptors, Somatotropin - metabolism</subject><subject>Sheep</subject><subject>Sheep Diseases - parasitology</subject><subject>somatotropin</subject><subject>veterinary parasitology</subject><issn>0304-4017</issn><issn>1873-2550</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhi1ERZeWN0DIRy5ZPHZiOxckVJUWqRKX9mx57VnFqyQOtrOItychhSNwGmv0_TMjf4S8BbYHBvLDaX_GMtm054zXe1h78gXZgVai4k3DXpIdE6yuagbqkrzO-cQYq5lUr8il0A3TrNU7gncpfi8d7WIa4og0lzDMvS2YaemQhpGeQ0mRejxjH6cBx0Lt6CnmYg99yN2vTjzSe4vLANfNmbo4lpjK8lriuUOcrsnF0fYZ3zzXK_L0-fbx5r56-Hr35ebTQ-WEUqXC2gsHGhwqacEv9wonAZSHBgXT3B4FByd9AwctUFtUSmjHnZItVwK5uCLvt7lTit_m5UYzhOyw7-2Icc5GQCOUBgntf6GNbNfyL5S3SgBrar2i9Ya6FHNOeDRTCoNNPwwwsyoyJ7NpM6s2s2lbYu-eN8yHAf2f0G9PC_BxA3D5vXPAZLILODr0IaErxsfw9w0_AYARqsI</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Sánchez-Paredes, Adolfo</creator><creator>Cuenca-Verde, César</creator><creator>Prado-Ochoa, María Guadalupe</creator><creator>Morales-Montor, Jorge</creator><creator>Alba-Hurtado, Fernando</creator><creator>Muñoz-Guzmán, Marco Antonio</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240601</creationdate><title>Growth hormone stimulates the in vitro development and establishment of Haemonchus contortus in sheep</title><author>Sánchez-Paredes, Adolfo ; Cuenca-Verde, César ; Prado-Ochoa, María Guadalupe ; Morales-Montor, Jorge ; Alba-Hurtado, Fernando ; Muñoz-Guzmán, Marco Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-e4d3c181ce76a1d0003c6117d15e3082af321c6d51b83e8ae7738c2c769273e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>abomasum</topic><topic>adults</topic><topic>Animals</topic><topic>confocal microscopy</topic><topic>Experimental infection</topic><topic>Female</topic><topic>females</topic><topic>flow cytometry</topic><topic>fluorescent antibody technique</topic><topic>Growth hormone</topic><topic>Growth Hormone - pharmacology</topic><topic>Haemonchiasis - parasitology</topic><topic>Haemonchiasis - veterinary</topic><topic>Haemonchus - drug effects</topic><topic>Haemonchus contortus</topic><topic>Hormonal receptor</topic><topic>intestines</topic><topic>Larva - drug effects</topic><topic>Larva - growth & development</topic><topic>Male</topic><topic>parasite load</topic><topic>parasites</topic><topic>prolactin</topic><topic>Receptors, Somatotropin - metabolism</topic><topic>Sheep</topic><topic>Sheep Diseases - parasitology</topic><topic>somatotropin</topic><topic>veterinary parasitology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sánchez-Paredes, Adolfo</creatorcontrib><creatorcontrib>Cuenca-Verde, César</creatorcontrib><creatorcontrib>Prado-Ochoa, María Guadalupe</creatorcontrib><creatorcontrib>Morales-Montor, Jorge</creatorcontrib><creatorcontrib>Alba-Hurtado, Fernando</creatorcontrib><creatorcontrib>Muñoz-Guzmán, Marco Antonio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sánchez-Paredes, Adolfo</au><au>Cuenca-Verde, César</au><au>Prado-Ochoa, María Guadalupe</au><au>Morales-Montor, Jorge</au><au>Alba-Hurtado, Fernando</au><au>Muñoz-Guzmán, Marco Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth hormone stimulates the in vitro development and establishment of Haemonchus contortus in sheep</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2024-06-01</date><risdate>2024</risdate><volume>328</volume><spage>110166</spage><pages>110166-</pages><artnum>110166</artnum><issn>0304-4017</issn><issn>1873-2550</issn><eissn>1873-2550</eissn><abstract>The physiologic increase in some sex hormones has been associated with an increase in the parasite load caused by Haemonchus contortus in ewes, especially prolactin. In lambs that are especially susceptible to hemonchosis, the levels of sex hormones are low; in contrast, the levels of another pituitary hormone, growth hormone (GH), which is structurally very similar to prolactin, are high. In this study, the in vitro and in vivo effects of GH on H. contortus larvae development and establishment were evaluated. The addition of 20 ng/mL GH for 5 and 10 days to cultures of H. contortus larvae induced an enlargement (p<0.01) and an L3/L4 molting rate (p<0.03) greater than that of untreated larvae or those treated with other concentrations of the hormone. Flow cytometry showed that 3.8% of the largest and most complex cells of newly obtained larvae of H. contortus were positive for the GH receptor, and by immunofluorescence with confocal microscopy, it was observed that these receptors are located in the intestinal region larvae. In the in vivo assay, the administration of recombinant GH to gonadectomized lambs produced an increase in FEC (p<0.03), the number of female adult worms in the abomasum (p<0.05) and the levels of specific antibodies (p<0.04) in relation to the control lambs; however, it did not affect the fertility of H. contortus females. Although many factors affect the development and implantation of H. contortus in the abomasum of sheep, the results of this study strongly suggest that GH participates in the development and establishment of the parasite in sheep, mainly in young sheep.
[Display omitted]
•In vitro and in vivo effects of growth hormone (GH) on H. contortus were evaluated.•In vitro exposure to GH increased elargement and molting in H. contortus larvae.•3.8% of cells of larvae of H. contortus showed like-receptors for GH.•GH increased parasite load and antibodies levels in lambs infected with H.contortus.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>38508098</pmid><doi>10.1016/j.vetpar.2024.110166</doi></addata></record> |
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| subjects | abomasum adults Animals confocal microscopy Experimental infection Female females flow cytometry fluorescent antibody technique Growth hormone Growth Hormone - pharmacology Haemonchiasis - parasitology Haemonchiasis - veterinary Haemonchus - drug effects Haemonchus contortus Hormonal receptor intestines Larva - drug effects Larva - growth & development Male parasite load parasites prolactin Receptors, Somatotropin - metabolism Sheep Sheep Diseases - parasitology somatotropin veterinary parasitology |
| title | Growth hormone stimulates the in vitro development and establishment of Haemonchus contortus in sheep |
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