The transcription factors NR5A1 and JUNB cooperate to activate the Axl promoter in mouse Sertoli cell lines
Background The Axl gene is a receptor tyrosine kinase essential for male fertility. With other Tyro3 family members, it regulates cell apoptosis and preserves the organization of seminiferous tubules. However, the regulation of the expression of Axl in testicular Sertoli cells is not entirely unders...
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| creator | Diawara, Mariama Martin, Luc J. |
| description | Background
The
Axl
gene is a receptor tyrosine kinase essential for male fertility. With other Tyro3 family members, it regulates cell apoptosis and preserves the organization of seminiferous tubules. However, the regulation of the expression of
Axl
in testicular Sertoli cells is not entirely understood. The transcription factors NR5A1 and JUNB are involved in several male fertility mechanisms such as sex development and steroidogenesis. We hypothesize that
Axl
promoter activity is regulated by cooperation between JUNB and NR5A1 in Sertoli cells.
Methods and Results
Following transfections of TM4 Sertoli cells with DsiRNA interference against
Axl
, our results show that cell morphology may be regulated by AXL
.
Using transfections of expression plasmids and reporter plasmids containing the
Axl
promoter, we report that
Axl
expression is highly activated by cooperation between NR5A1 and JUNB in TM4 and 15P-1 Sertoli cells. Chromatin immunoprecipitation and luciferase reporter assays with 5′ promoter deletions demonstrate that JUNB and NR5A1 are being recruited to DNA regulatory elements in the proximal region of the
Axl
promoter. The fourth intronic region of
Axl
also participates in the recruitment of JUNB.
Conclusion
Thus,
Axl
expression is regulated by a cooperation between the transcription factors JUNB and NR5A1 and influences the morphology of TM4 Sertoli cells. |
| doi_str_mv | 10.1007/s11033-024-09934-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153771634</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3153771634</sourcerecordid><originalsourceid>FETCH-LOGICAL-c289t-d700730cd6264ebc65c8beeb854c116fef9606e40fe20eeaab25c0eaaca58e7d3</originalsourceid><addsrcrecordid>eNqNkUtP3DAUha2qCKbAH-iistQNm8B1_EqW0xG0IDRIPNaW49y0mSbx1M4g-Pf1PApSF4jVvZK_c3yuDiGfGZwyAH0WGQPOM8hFBmXJRcY_kAmTmmei1MVHMgEOLBOFZAfkU4wLABBMy31ywMtcMynLCfl9_wvpGOwQXWiXY-sH2lg3-hDp_FZOGbVDTa8e5t-o836JwY4J9zQh7eNmT_LpU0eXwfd-xEDbgfZ-FZHeYRh911KHXUe7dsB4RPYa20U83s1D8nBxfj_7kV3ffL-cTa8zlxflmNU6HcfB1SpXAiunpCsqxKqQwjGmGmxKBQoFNJgDorVVLh2k6awsUNf8kJxsfVOoPyuMo-nbuI5hB0zRDGeSa80UF-9AQUheinyNfv0PXfhVGNIhG4orKNSayreUCz7GgI1Zhra34dkwMOvWzLY1k1ozm9YMT6IvO-tV1WP9IvlXUwL4FojpafiJ4fXvN2z_Ahqrof8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3104360864</pqid></control><display><type>article</type><title>The transcription factors NR5A1 and JUNB cooperate to activate the Axl promoter in mouse Sertoli cell lines</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Diawara, Mariama ; Martin, Luc J.</creator><creatorcontrib>Diawara, Mariama ; Martin, Luc J.</creatorcontrib><description>Background
The
Axl
gene is a receptor tyrosine kinase essential for male fertility. With other Tyro3 family members, it regulates cell apoptosis and preserves the organization of seminiferous tubules. However, the regulation of the expression of
Axl
in testicular Sertoli cells is not entirely understood. The transcription factors NR5A1 and JUNB are involved in several male fertility mechanisms such as sex development and steroidogenesis. We hypothesize that
Axl
promoter activity is regulated by cooperation between JUNB and NR5A1 in Sertoli cells.
Methods and Results
Following transfections of TM4 Sertoli cells with DsiRNA interference against
Axl
, our results show that cell morphology may be regulated by AXL
.
Using transfections of expression plasmids and reporter plasmids containing the
Axl
promoter, we report that
Axl
expression is highly activated by cooperation between NR5A1 and JUNB in TM4 and 15P-1 Sertoli cells. Chromatin immunoprecipitation and luciferase reporter assays with 5′ promoter deletions demonstrate that JUNB and NR5A1 are being recruited to DNA regulatory elements in the proximal region of the
Axl
promoter. The fourth intronic region of
Axl
also participates in the recruitment of JUNB.
Conclusion
Thus,
Axl
expression is regulated by a cooperation between the transcription factors JUNB and NR5A1 and influences the morphology of TM4 Sertoli cells.</description><identifier>ISSN: 0301-4851</identifier><identifier>ISSN: 1573-4978</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-024-09934-3</identifier><identifier>PMID: 39271559</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Animals ; Apoptosis ; Axl protein ; Axl Receptor Tyrosine Kinase ; Biomedical and Life Sciences ; Brief Report ; Cell Line ; Cell lines ; Cell morphology ; cell structures ; Chromatin ; chromatin immunoprecipitation ; Cooperation ; family ; Fertility ; Gene Expression Regulation ; Gene regulation ; Histology ; Immunoprecipitation ; introns ; JunB protein ; Kinases ; Life Sciences ; luciferase ; Male ; male fertility ; Mice ; Morphology ; Plasmids ; Promoter Regions, Genetic - genetics ; Protein-tyrosine kinase receptors ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins - metabolism ; receptor protein-tyrosine kinase ; Receptor Protein-Tyrosine Kinases - genetics ; Receptor Protein-Tyrosine Kinases - metabolism ; Regulatory sequences ; Sertoli cells ; Sertoli Cells - metabolism ; Steroidogenesis ; Steroidogenic Factor 1 - genetics ; Steroidogenic Factor 1 - metabolism ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Tubules</subject><ispartof>Molecular biology reports, 2024-12, Vol.51 (1), p.982-982, Article 982</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024 Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><rights>Copyright Springer Nature B.V. Dec 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c289t-d700730cd6264ebc65c8beeb854c116fef9606e40fe20eeaab25c0eaaca58e7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-024-09934-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-024-09934-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39271559$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Diawara, Mariama</creatorcontrib><creatorcontrib>Martin, Luc J.</creatorcontrib><title>The transcription factors NR5A1 and JUNB cooperate to activate the Axl promoter in mouse Sertoli cell lines</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
The
Axl
gene is a receptor tyrosine kinase essential for male fertility. With other Tyro3 family members, it regulates cell apoptosis and preserves the organization of seminiferous tubules. However, the regulation of the expression of
Axl
in testicular Sertoli cells is not entirely understood. The transcription factors NR5A1 and JUNB are involved in several male fertility mechanisms such as sex development and steroidogenesis. We hypothesize that
Axl
promoter activity is regulated by cooperation between JUNB and NR5A1 in Sertoli cells.
Methods and Results
Following transfections of TM4 Sertoli cells with DsiRNA interference against
Axl
, our results show that cell morphology may be regulated by AXL
.
Using transfections of expression plasmids and reporter plasmids containing the
Axl
promoter, we report that
Axl
expression is highly activated by cooperation between NR5A1 and JUNB in TM4 and 15P-1 Sertoli cells. Chromatin immunoprecipitation and luciferase reporter assays with 5′ promoter deletions demonstrate that JUNB and NR5A1 are being recruited to DNA regulatory elements in the proximal region of the
Axl
promoter. The fourth intronic region of
Axl
also participates in the recruitment of JUNB.
Conclusion
Thus,
Axl
expression is regulated by a cooperation between the transcription factors JUNB and NR5A1 and influences the morphology of TM4 Sertoli cells.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Axl protein</subject><subject>Axl Receptor Tyrosine Kinase</subject><subject>Biomedical and Life Sciences</subject><subject>Brief Report</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell morphology</subject><subject>cell structures</subject><subject>Chromatin</subject><subject>chromatin immunoprecipitation</subject><subject>Cooperation</subject><subject>family</subject><subject>Fertility</subject><subject>Gene Expression Regulation</subject><subject>Gene regulation</subject><subject>Histology</subject><subject>Immunoprecipitation</subject><subject>introns</subject><subject>JunB protein</subject><subject>Kinases</subject><subject>Life Sciences</subject><subject>luciferase</subject><subject>Male</subject><subject>male fertility</subject><subject>Mice</subject><subject>Morphology</subject><subject>Plasmids</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein-tyrosine kinase receptors</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>receptor protein-tyrosine kinase</subject><subject>Receptor Protein-Tyrosine Kinases - genetics</subject><subject>Receptor Protein-Tyrosine Kinases - metabolism</subject><subject>Regulatory sequences</subject><subject>Sertoli cells</subject><subject>Sertoli Cells - metabolism</subject><subject>Steroidogenesis</subject><subject>Steroidogenic Factor 1 - genetics</subject><subject>Steroidogenic Factor 1 - metabolism</subject><subject>Transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Tubules</subject><issn>0301-4851</issn><issn>1573-4978</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtP3DAUha2qCKbAH-iistQNm8B1_EqW0xG0IDRIPNaW49y0mSbx1M4g-Pf1PApSF4jVvZK_c3yuDiGfGZwyAH0WGQPOM8hFBmXJRcY_kAmTmmei1MVHMgEOLBOFZAfkU4wLABBMy31ywMtcMynLCfl9_wvpGOwQXWiXY-sH2lg3-hDp_FZOGbVDTa8e5t-o836JwY4J9zQh7eNmT_LpU0eXwfd-xEDbgfZ-FZHeYRh911KHXUe7dsB4RPYa20U83s1D8nBxfj_7kV3ffL-cTa8zlxflmNU6HcfB1SpXAiunpCsqxKqQwjGmGmxKBQoFNJgDorVVLh2k6awsUNf8kJxsfVOoPyuMo-nbuI5hB0zRDGeSa80UF-9AQUheinyNfv0PXfhVGNIhG4orKNSayreUCz7GgI1Zhra34dkwMOvWzLY1k1ozm9YMT6IvO-tV1WP9IvlXUwL4FojpafiJ4fXvN2z_Ahqrof8</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Diawara, Mariama</creator><creator>Martin, Luc J.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20241201</creationdate><title>The transcription factors NR5A1 and JUNB cooperate to activate the Axl promoter in mouse Sertoli cell lines</title><author>Diawara, Mariama ; Martin, Luc J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c289t-d700730cd6264ebc65c8beeb854c116fef9606e40fe20eeaab25c0eaaca58e7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Axl protein</topic><topic>Axl Receptor Tyrosine Kinase</topic><topic>Biomedical and Life Sciences</topic><topic>Brief Report</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cell morphology</topic><topic>cell structures</topic><topic>Chromatin</topic><topic>chromatin immunoprecipitation</topic><topic>Cooperation</topic><topic>family</topic><topic>Fertility</topic><topic>Gene Expression Regulation</topic><topic>Gene regulation</topic><topic>Histology</topic><topic>Immunoprecipitation</topic><topic>introns</topic><topic>JunB protein</topic><topic>Kinases</topic><topic>Life Sciences</topic><topic>luciferase</topic><topic>Male</topic><topic>male fertility</topic><topic>Mice</topic><topic>Morphology</topic><topic>Plasmids</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protein-tyrosine kinase receptors</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>receptor protein-tyrosine kinase</topic><topic>Receptor Protein-Tyrosine Kinases - genetics</topic><topic>Receptor Protein-Tyrosine Kinases - metabolism</topic><topic>Regulatory sequences</topic><topic>Sertoli cells</topic><topic>Sertoli Cells - metabolism</topic><topic>Steroidogenesis</topic><topic>Steroidogenic Factor 1 - genetics</topic><topic>Steroidogenic Factor 1 - metabolism</topic><topic>Transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Tubules</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Diawara, Mariama</creatorcontrib><creatorcontrib>Martin, Luc J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Diawara, Mariama</au><au>Martin, Luc J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The transcription factors NR5A1 and JUNB cooperate to activate the Axl promoter in mouse Sertoli cell lines</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>51</volume><issue>1</issue><spage>982</spage><epage>982</epage><pages>982-982</pages><artnum>982</artnum><issn>0301-4851</issn><issn>1573-4978</issn><eissn>1573-4978</eissn><abstract>Background
The
Axl
gene is a receptor tyrosine kinase essential for male fertility. With other Tyro3 family members, it regulates cell apoptosis and preserves the organization of seminiferous tubules. However, the regulation of the expression of
Axl
in testicular Sertoli cells is not entirely understood. The transcription factors NR5A1 and JUNB are involved in several male fertility mechanisms such as sex development and steroidogenesis. We hypothesize that
Axl
promoter activity is regulated by cooperation between JUNB and NR5A1 in Sertoli cells.
Methods and Results
Following transfections of TM4 Sertoli cells with DsiRNA interference against
Axl
, our results show that cell morphology may be regulated by AXL
.
Using transfections of expression plasmids and reporter plasmids containing the
Axl
promoter, we report that
Axl
expression is highly activated by cooperation between NR5A1 and JUNB in TM4 and 15P-1 Sertoli cells. Chromatin immunoprecipitation and luciferase reporter assays with 5′ promoter deletions demonstrate that JUNB and NR5A1 are being recruited to DNA regulatory elements in the proximal region of the
Axl
promoter. The fourth intronic region of
Axl
also participates in the recruitment of JUNB.
Conclusion
Thus,
Axl
expression is regulated by a cooperation between the transcription factors JUNB and NR5A1 and influences the morphology of TM4 Sertoli cells.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>39271559</pmid><doi>10.1007/s11033-024-09934-3</doi><tpages>1</tpages></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Animals Apoptosis Axl protein Axl Receptor Tyrosine Kinase Biomedical and Life Sciences Brief Report Cell Line Cell lines Cell morphology cell structures Chromatin chromatin immunoprecipitation Cooperation family Fertility Gene Expression Regulation Gene regulation Histology Immunoprecipitation introns JunB protein Kinases Life Sciences luciferase Male male fertility Mice Morphology Plasmids Promoter Regions, Genetic - genetics Protein-tyrosine kinase receptors Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism receptor protein-tyrosine kinase Receptor Protein-Tyrosine Kinases - genetics Receptor Protein-Tyrosine Kinases - metabolism Regulatory sequences Sertoli cells Sertoli Cells - metabolism Steroidogenesis Steroidogenic Factor 1 - genetics Steroidogenic Factor 1 - metabolism Transcription factors Transcription Factors - genetics Transcription Factors - metabolism Tubules |
| title | The transcription factors NR5A1 and JUNB cooperate to activate the Axl promoter in mouse Sertoli cell lines |
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