Matrix Metalloproteinase 11 is Overexpressed in Various Histotypes of Canine Mammary Tumors
Matrix metalloproteinase 11 (MMP-11) belongs to the family of matrix metalloproteinases whose serum level has been reported to be elevated in dogs with mammary tumors. The present investigation focuses on assessing the tissue level expression of MMP-11 in various histotypes of canine mammary tumors...
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| Veröffentlicht in: | National Academy science letters 2024-08, Vol.47 (4), p.433-436 |
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| creator | Sethi, Shefali Sunil Kumar, B. V. |
| description | Matrix metalloproteinase 11 (MMP-11) belongs to the family of matrix metalloproteinases whose serum level has been reported to be elevated in dogs with mammary tumors. The present investigation focuses on assessing the tissue level expression of MMP-11 in various histotypes of canine mammary tumors (CMT). In this study, 20 CMT tissue paraffin blocks preserved in the laboratory were used. Slides were prepared for histopathological examination and immunohistochemistry. Hyperimmune serum raised against the canine recombinant MMP-11 (rMMP-11) in mice was used to standardize immunohistochemistry to assess expression of MMP-11 protein in various histotypes of CMT. Based on the histopathological examination of hematoxylin and eosin (H&E)-stained tissue sections, the CMT tissues were classified into complex carcinoma, mixed mammary tumor, papillary carcinoma and anaplastic carcinoma. A strong MMP-11 expression was observed in 75% of the histotypes of CMT under study. The findings suggest that MMP-11 could act as molecular marker for the immunohistochemistry-based diagnosis of mammary tumors in dogs. |
| doi_str_mv | 10.1007/s40009-024-01393-5 |
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A strong MMP-11 expression was observed in 75% of the histotypes of CMT under study. 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V.</creatorcontrib><title>Matrix Metalloproteinase 11 is Overexpressed in Various Histotypes of Canine Mammary Tumors</title><title>National Academy science letters</title><addtitle>Natl. Acad. Sci. Lett</addtitle><description>Matrix metalloproteinase 11 (MMP-11) belongs to the family of matrix metalloproteinases whose serum level has been reported to be elevated in dogs with mammary tumors. The present investigation focuses on assessing the tissue level expression of MMP-11 in various histotypes of canine mammary tumors (CMT). In this study, 20 CMT tissue paraffin blocks preserved in the laboratory were used. Slides were prepared for histopathological examination and immunohistochemistry. Hyperimmune serum raised against the canine recombinant MMP-11 (rMMP-11) in mice was used to standardize immunohistochemistry to assess expression of MMP-11 protein in various histotypes of CMT. Based on the histopathological examination of hematoxylin and eosin (H&E)-stained tissue sections, the CMT tissues were classified into complex carcinoma, mixed mammary tumor, papillary carcinoma and anaplastic carcinoma. A strong MMP-11 expression was observed in 75% of the histotypes of CMT under study. The findings suggest that MMP-11 could act as molecular marker for the immunohistochemistry-based diagnosis of mammary tumors in dogs.</description><subject>antiserum</subject><subject>blood serum</subject><subject>Cancer</subject><subject>carcinoma</subject><subject>Dogs</subject><subject>eosin</subject><subject>family</subject><subject>genetic markers</subject><subject>histopathology</subject><subject>History of Science</subject><subject>Humanities and Social Sciences</subject><subject>Immunohistochemistry</subject><subject>Mammary gland</subject><subject>mammary neoplasms (animal)</subject><subject>Matrix metalloproteinase</subject><subject>Matrix metalloproteinases</subject><subject>Metalloproteinase</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Short Communication</subject><subject>stromelysin-3</subject><subject>Tumors</subject><issn>0250-541X</issn><issn>2250-1754</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kEFLw0AQhRdRsNT-AU8LXrxEZ7O72eQoRa3Q0ksVwcOyTSayJU3qTiLtvzexguDB08zhe4_3HmOXAm4EgLklBQBZBLGKQMhMRvqEjeJYQySMVqdsBMOvlXg9ZxOiTU-DTrQW8Yi9LVwb_J4vsHVV1exC06KvHSEXgnviy08MuN8FJMKC-5q_uOCbjvjMU9u0hx0Sb0o-dbWvkS_cduvCga-6bRPogp2VriKc_Nwxe364X01n0Xz5-DS9m0e5BNlGGWg0xkkVFwJTUTrtyiRXa1UmCkWWF2sV961AJYWURSlLlaZr1Ab1GkyWajlm10ffPv1Hh9Taraccq8rV2Ee1UmhpDGgpevTqD7ppulD36ayETOvMKDNQ8ZHKQ0MUsLS74IdiVoAdJrfHyW0_uf2e3A4p5FFEPVy_Y_i1_kf1BV2zgzc</recordid><startdate>20240801</startdate><enddate>20240801</enddate><creator>Sethi, Shefali</creator><creator>Sunil Kumar, B. 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V.</creatorcontrib><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>National Academy science letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sethi, Shefali</au><au>Sunil Kumar, B. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Matrix Metalloproteinase 11 is Overexpressed in Various Histotypes of Canine Mammary Tumors</atitle><jtitle>National Academy science letters</jtitle><stitle>Natl. Acad. Sci. Lett</stitle><date>2024-08-01</date><risdate>2024</risdate><volume>47</volume><issue>4</issue><spage>433</spage><epage>436</epage><pages>433-436</pages><issn>0250-541X</issn><eissn>2250-1754</eissn><abstract>Matrix metalloproteinase 11 (MMP-11) belongs to the family of matrix metalloproteinases whose serum level has been reported to be elevated in dogs with mammary tumors. The present investigation focuses on assessing the tissue level expression of MMP-11 in various histotypes of canine mammary tumors (CMT). In this study, 20 CMT tissue paraffin blocks preserved in the laboratory were used. Slides were prepared for histopathological examination and immunohistochemistry. Hyperimmune serum raised against the canine recombinant MMP-11 (rMMP-11) in mice was used to standardize immunohistochemistry to assess expression of MMP-11 protein in various histotypes of CMT. Based on the histopathological examination of hematoxylin and eosin (H&E)-stained tissue sections, the CMT tissues were classified into complex carcinoma, mixed mammary tumor, papillary carcinoma and anaplastic carcinoma. A strong MMP-11 expression was observed in 75% of the histotypes of CMT under study. The findings suggest that MMP-11 could act as molecular marker for the immunohistochemistry-based diagnosis of mammary tumors in dogs.</abstract><cop>New Delhi</cop><pub>Springer India</pub><doi>10.1007/s40009-024-01393-5</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0003-2641-6859</orcidid></addata></record> |
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| subjects | antiserum blood serum Cancer carcinoma Dogs eosin family genetic markers histopathology History of Science Humanities and Social Sciences Immunohistochemistry Mammary gland mammary neoplasms (animal) Matrix metalloproteinase Matrix metalloproteinases Metalloproteinase multidisciplinary Science Science (multidisciplinary) Short Communication stromelysin-3 Tumors |
| title | Matrix Metalloproteinase 11 is Overexpressed in Various Histotypes of Canine Mammary Tumors |
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