CRISPR/Cas12a and Hybridization Chain Reaction-Coregulated Magnetic Relaxation Switching Biosensor for Sensitive Detection of Viable Salmonella in Animal-Derived Foods

We combined a CRISPR/Cas12a system with a hybridization chain reaction (HCR) to develop an ultrasensitive magnetic relaxation switching (MRS) biosensor for detecting viable Salmonella typhimurium (S. typhimurium). Magnetic nanoparticles of two sizes (30 and 1000 nm: MNP30 and MNP1000, respectively)...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2024-09, Vol.72 (36), p.20130-20139
Hauptverfasser:	Gu, Aoting, Dong, Yongzhen, Li, Letian, Yu, Deyang, Zhang, Jiangjiang, Chen, Yiping
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Biosensing Techniques - instrumentation Biosensing Techniques - methods biosensors CRISPR-Cas Systems detection limit food chemistry Food Contamination - analysis Food Microbiology - methods genes hybridization hybridization chain reaction Limit of Detection magnetic separation magnetism nanoparticles New Analytical Methods Nucleic Acid Hybridization Salmonella Typhimurium Salmonella typhimurium - genetics Salmonella typhimurium - isolation & purification Swine
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