GmIRT1.1 from soybean (Glycine max L.) is involved in transporting Fe, Mn and Cd
Soybean is one of the most important crops for producing high quality oil and protein. Mineral nutrient deficiencies are frequently observed in soybeans. However, there are few studies to understand the absorption process of mineral nutrients in soybeans. Here, we investigated the functions of soybe...
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| Veröffentlicht in: | Plant physiology and biochemistry 2024-10, Vol.215, p.109010, Article 109010 |
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| creator | Gong, Changyi Yin, Xinghua Cheng, Liqing Huang, Yunfeng Shi, Renkui Xie, Mengya Yang, Guang Kong, Linghui Zhang, Wei Chen, Xi |
| description | Soybean is one of the most important crops for producing high quality oil and protein. Mineral nutrient deficiencies are frequently observed in soybeans. However, there are few studies to understand the absorption process of mineral nutrients in soybeans. Here, we investigated the functions of soybean (Glycine max L.) IRT1.1 (IRON-REGULATED TRANSPORTER 1.1) in the transportation of mineral elements. Heterologous expression of GmIRT1.1 in yeast mutants revealed that GmIRT1.1 compensated for the growth defects of Δfet3fet4 and Δsmf1 mutants under iron (Fe) and manganese (Mn) deficiency conditions, respectively, and enhanced the sensitivity of the Δycf1 mutant to cadmium (Cd) toxicity. Expression analysis revealed that GmIRT1.1 was only significantly induced by Fe deficiency and was primarily expressed in roots. Furthermore, the GmIRT1.1 overexpression lines enhanced Arabidopsis tolerance to Fe deficiency, leading to increased accumulation of Fe in the roots and shoots. Additionally, the transgenic lines increased the sensitivity to Mn and Cd toxicity. Subcellular localization analysis revealed that GmIRT1.1 was localized on the plasma membrane. Moreover, the results obtained from the soybean hairy roots system indicated that the localization of GmIRT1.1 was dependent on the regulation of Fe homeostasis in plant. Consequently, these results suggested that GmIRT1.1 was responsible for the transportation of Fe, Mn and Cd.
•Heterologous expression of GmIRT1.1 in yeast mutants revealed that GmIRT1.1 could transport Fe, Mn and Cd.•Subcellular localization analysis indicated that GmIRT1.1 was localized on the plasma membrane.•Expression analysis revealed that GmIRT1.1 was only significantly induced by Fe deficiency and was primarily expressed in roots.•Results obtained from the soybean hairy roots system revealed that the localization of GmIRT1.1 was dependent on the regulation of Fe homeostasis in plant.•Heterologous expression of GmIRT1.1 in Arabidopsis can enhance plant growth in condition of Fe deficiency, and increase plant sensitivity to Mn and Cd toxicity. |
| doi_str_mv | 10.1016/j.plaphy.2024.109010 |
| format | Article |
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•Heterologous expression of GmIRT1.1 in yeast mutants revealed that GmIRT1.1 could transport Fe, Mn and Cd.•Subcellular localization analysis indicated that GmIRT1.1 was localized on the plasma membrane.•Expression analysis revealed that GmIRT1.1 was only significantly induced by Fe deficiency and was primarily expressed in roots.•Results obtained from the soybean hairy roots system revealed that the localization of GmIRT1.1 was dependent on the regulation of Fe homeostasis in plant.•Heterologous expression of GmIRT1.1 in Arabidopsis can enhance plant growth in condition of Fe deficiency, and increase plant sensitivity to Mn and Cd toxicity.</description><identifier>ISSN: 0981-9428</identifier><identifier>ISSN: 1873-2690</identifier><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/j.plaphy.2024.109010</identifier><identifier>PMID: 39146910</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>absorption ; Arabidopsis ; Cadmium ; genetically modified organisms ; Glycine max ; Glycine max L ; heterologous gene expression ; homeostasis ; Iron ; Manganese ; mutants ; nutrient deficiencies ; oils ; plant physiology ; plasma membrane ; soybeans ; toxicity ; Transport ; transportation ; yeasts</subject><ispartof>Plant physiology and biochemistry, 2024-10, Vol.215, p.109010, Article 109010</ispartof><rights>2024 Elsevier Masson SAS</rights><rights>Copyright © 2024 Elsevier Masson SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c274t-f1a596491bac2771e9eba2b100d788c47c90d5c7c4533556f058a918770c46563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0981942824006788$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39146910$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gong, Changyi</creatorcontrib><creatorcontrib>Yin, Xinghua</creatorcontrib><creatorcontrib>Cheng, Liqing</creatorcontrib><creatorcontrib>Huang, Yunfeng</creatorcontrib><creatorcontrib>Shi, Renkui</creatorcontrib><creatorcontrib>Xie, Mengya</creatorcontrib><creatorcontrib>Yang, Guang</creatorcontrib><creatorcontrib>Kong, Linghui</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><title>GmIRT1.1 from soybean (Glycine max L.) is involved in transporting Fe, Mn and Cd</title><title>Plant physiology and biochemistry</title><addtitle>Plant Physiol Biochem</addtitle><description>Soybean is one of the most important crops for producing high quality oil and protein. Mineral nutrient deficiencies are frequently observed in soybeans. However, there are few studies to understand the absorption process of mineral nutrients in soybeans. Here, we investigated the functions of soybean (Glycine max L.) IRT1.1 (IRON-REGULATED TRANSPORTER 1.1) in the transportation of mineral elements. Heterologous expression of GmIRT1.1 in yeast mutants revealed that GmIRT1.1 compensated for the growth defects of Δfet3fet4 and Δsmf1 mutants under iron (Fe) and manganese (Mn) deficiency conditions, respectively, and enhanced the sensitivity of the Δycf1 mutant to cadmium (Cd) toxicity. Expression analysis revealed that GmIRT1.1 was only significantly induced by Fe deficiency and was primarily expressed in roots. Furthermore, the GmIRT1.1 overexpression lines enhanced Arabidopsis tolerance to Fe deficiency, leading to increased accumulation of Fe in the roots and shoots. Additionally, the transgenic lines increased the sensitivity to Mn and Cd toxicity. Subcellular localization analysis revealed that GmIRT1.1 was localized on the plasma membrane. Moreover, the results obtained from the soybean hairy roots system indicated that the localization of GmIRT1.1 was dependent on the regulation of Fe homeostasis in plant. Consequently, these results suggested that GmIRT1.1 was responsible for the transportation of Fe, Mn and Cd.
•Heterologous expression of GmIRT1.1 in yeast mutants revealed that GmIRT1.1 could transport Fe, Mn and Cd.•Subcellular localization analysis indicated that GmIRT1.1 was localized on the plasma membrane.•Expression analysis revealed that GmIRT1.1 was only significantly induced by Fe deficiency and was primarily expressed in roots.•Results obtained from the soybean hairy roots system revealed that the localization of GmIRT1.1 was dependent on the regulation of Fe homeostasis in plant.•Heterologous expression of GmIRT1.1 in Arabidopsis can enhance plant growth in condition of Fe deficiency, and increase plant sensitivity to Mn and Cd toxicity.</description><subject>absorption</subject><subject>Arabidopsis</subject><subject>Cadmium</subject><subject>genetically modified organisms</subject><subject>Glycine max</subject><subject>Glycine max L</subject><subject>heterologous gene expression</subject><subject>homeostasis</subject><subject>Iron</subject><subject>Manganese</subject><subject>mutants</subject><subject>nutrient deficiencies</subject><subject>oils</subject><subject>plant physiology</subject><subject>plasma membrane</subject><subject>soybeans</subject><subject>toxicity</subject><subject>Transport</subject><subject>transportation</subject><subject>yeasts</subject><issn>0981-9428</issn><issn>1873-2690</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkFGL1DAQx4Mo3rr6DUTyeIKtmSZpmhdBFm89WFHkfA5pOtUsbVqT7uJ-e3P09FGfZhh-M3_mR8hLYCUwqN8ey3mw849LWbFK5JFmwB6RDTSKF1Wt2WOyYbqBQouquSLPUjoylknFn5IrrkHUGtiGfNmPt1_voATax2mkabq0aAO93g8X5wPS0f6ih_I19Yn6cJ6GM3a5oUu0Ic1TXHz4Tm_wDf0UqA0d3XXPyZPeDglfPNQt-Xbz4W73sTh83t_u3h8KVymxFD1YqWuhobV5oAA1trZqgbFONY0TymnWSaeckJxLWfdMNlbn5xRzopY135Lr9e4cp58nTIsZfXI4DDbgdEqGg-RKqAb0_1GmudSVzklbIlbUxSmliL2Zox9tvBhg5l67OZpVu7nXblbtee3VQ8KpHbH7u_THcwberQBmJWeP0STnMTjsfES3mG7y_074DRdHkPw</recordid><startdate>20241001</startdate><enddate>20241001</enddate><creator>Gong, Changyi</creator><creator>Yin, Xinghua</creator><creator>Cheng, Liqing</creator><creator>Huang, Yunfeng</creator><creator>Shi, Renkui</creator><creator>Xie, Mengya</creator><creator>Yang, Guang</creator><creator>Kong, Linghui</creator><creator>Zhang, Wei</creator><creator>Chen, Xi</creator><general>Elsevier Masson SAS</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20241001</creationdate><title>GmIRT1.1 from soybean (Glycine max L.) is involved in transporting Fe, Mn and Cd</title><author>Gong, Changyi ; Yin, Xinghua ; Cheng, Liqing ; Huang, Yunfeng ; Shi, Renkui ; Xie, Mengya ; Yang, Guang ; Kong, Linghui ; Zhang, Wei ; Chen, Xi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c274t-f1a596491bac2771e9eba2b100d788c47c90d5c7c4533556f058a918770c46563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>absorption</topic><topic>Arabidopsis</topic><topic>Cadmium</topic><topic>genetically modified organisms</topic><topic>Glycine max</topic><topic>Glycine max L</topic><topic>heterologous gene expression</topic><topic>homeostasis</topic><topic>Iron</topic><topic>Manganese</topic><topic>mutants</topic><topic>nutrient deficiencies</topic><topic>oils</topic><topic>plant physiology</topic><topic>plasma membrane</topic><topic>soybeans</topic><topic>toxicity</topic><topic>Transport</topic><topic>transportation</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gong, Changyi</creatorcontrib><creatorcontrib>Yin, Xinghua</creatorcontrib><creatorcontrib>Cheng, Liqing</creatorcontrib><creatorcontrib>Huang, Yunfeng</creatorcontrib><creatorcontrib>Shi, Renkui</creatorcontrib><creatorcontrib>Xie, Mengya</creatorcontrib><creatorcontrib>Yang, Guang</creatorcontrib><creatorcontrib>Kong, Linghui</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Changyi</au><au>Yin, Xinghua</au><au>Cheng, Liqing</au><au>Huang, Yunfeng</au><au>Shi, Renkui</au><au>Xie, Mengya</au><au>Yang, Guang</au><au>Kong, Linghui</au><au>Zhang, Wei</au><au>Chen, Xi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GmIRT1.1 from soybean (Glycine max L.) is involved in transporting Fe, Mn and Cd</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2024-10-01</date><risdate>2024</risdate><volume>215</volume><spage>109010</spage><pages>109010-</pages><artnum>109010</artnum><issn>0981-9428</issn><issn>1873-2690</issn><eissn>1873-2690</eissn><abstract>Soybean is one of the most important crops for producing high quality oil and protein. Mineral nutrient deficiencies are frequently observed in soybeans. However, there are few studies to understand the absorption process of mineral nutrients in soybeans. Here, we investigated the functions of soybean (Glycine max L.) IRT1.1 (IRON-REGULATED TRANSPORTER 1.1) in the transportation of mineral elements. Heterologous expression of GmIRT1.1 in yeast mutants revealed that GmIRT1.1 compensated for the growth defects of Δfet3fet4 and Δsmf1 mutants under iron (Fe) and manganese (Mn) deficiency conditions, respectively, and enhanced the sensitivity of the Δycf1 mutant to cadmium (Cd) toxicity. Expression analysis revealed that GmIRT1.1 was only significantly induced by Fe deficiency and was primarily expressed in roots. Furthermore, the GmIRT1.1 overexpression lines enhanced Arabidopsis tolerance to Fe deficiency, leading to increased accumulation of Fe in the roots and shoots. Additionally, the transgenic lines increased the sensitivity to Mn and Cd toxicity. Subcellular localization analysis revealed that GmIRT1.1 was localized on the plasma membrane. Moreover, the results obtained from the soybean hairy roots system indicated that the localization of GmIRT1.1 was dependent on the regulation of Fe homeostasis in plant. Consequently, these results suggested that GmIRT1.1 was responsible for the transportation of Fe, Mn and Cd.
•Heterologous expression of GmIRT1.1 in yeast mutants revealed that GmIRT1.1 could transport Fe, Mn and Cd.•Subcellular localization analysis indicated that GmIRT1.1 was localized on the plasma membrane.•Expression analysis revealed that GmIRT1.1 was only significantly induced by Fe deficiency and was primarily expressed in roots.•Results obtained from the soybean hairy roots system revealed that the localization of GmIRT1.1 was dependent on the regulation of Fe homeostasis in plant.•Heterologous expression of GmIRT1.1 in Arabidopsis can enhance plant growth in condition of Fe deficiency, and increase plant sensitivity to Mn and Cd toxicity.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>39146910</pmid><doi>10.1016/j.plaphy.2024.109010</doi></addata></record> |
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| subjects | absorption Arabidopsis Cadmium genetically modified organisms Glycine max Glycine max L heterologous gene expression homeostasis Iron Manganese mutants nutrient deficiencies oils plant physiology plasma membrane soybeans toxicity Transport transportation yeasts |
| title | GmIRT1.1 from soybean (Glycine max L.) is involved in transporting Fe, Mn and Cd |
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