Evaluating insulindysregulation in horses: A two-step insulin-tolerance test using porcine zinc insulin

In insulin dysregulation, hyperinsulinemia (HI) can be accompanied by peripheral insulin resistance (IR) in horses, which can be diagnosed with an insulin-tolerance test (ITT). The administration of 0.1 IU/kg body weight of recombinant regular human insulin (RHI) should elicit a 50 % reduction of th...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The veterinary journal (1997) 2024-08, Vol.306, p.106172, Article 106172
Hauptverfasser: Grob, A.J., Delarocque, J., Feige, K., Warnken, T.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page 106172
container_title The veterinary journal (1997)
container_volume 306
creator Grob, A.J.
Delarocque, J.
Feige, K.
Warnken, T.
description In insulin dysregulation, hyperinsulinemia (HI) can be accompanied by peripheral insulin resistance (IR) in horses, which can be diagnosed with an insulin-tolerance test (ITT). The administration of 0.1 IU/kg body weight of recombinant regular human insulin (RHI) should elicit a 50 % reduction of the initial blood glucose concentration at 30 min after insulin administration in insulin sensitive horses. Compared to RHI, porcine zinc insulin (PZI) is veterinary-approved and therefore easier accessible for many practitioners. The aim of this study was to compare the insulin and glucose dynamics during a standard ITT with RHI to an ITT performed with PZI. Twelve Icelandic horses were subjected to an ITT with RHI (ITT-RHI) and with PZI (ITT-PZI) at same dosages in a randomised crossover design. The insulin and glucose dynamics that resulted from these tests were compared, and the consistency of classification into insulin-sensitive and IR categories was evaluated. No complications were observed with the use of either RHI or PZI in ITT. A good correlation of the test results was observed (r = 0.88; P < 0.001). The blood glucose concentrations and the percentage reduction in glucose concentration did not differ significantly between the two tests (P = 0.053), but four out of twelve horses were classified as IR in the ITT-RHI whereas with the ITT-PZI seven out of twelve horses were classified as IR with the 50 % glucose reduction from baseline. Based on the Youden index, when using the ITT-PZI, an adjusted cut-off value for blood glucose reduction of 40 % at 30 min resulted in better test performance. With consideration for the seemingly weaker effect of PZI and the adjusted cut-off value, PZI can be an appropriate substitute to RHI in an ITT. •Zinc insulin (PZI) could be used in an insulin-response test (IRT) in horses.•PZI used intravenously seemed less effective compared to regular insulin.•A 40 % blood glucose reduction was indicative of insulin resistance in IRT with PZI.
doi_str_mv 10.1016/j.tvjl.2024.106172
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153737950</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1090023324001114</els_id><sourcerecordid>3071512958</sourcerecordid><originalsourceid>FETCH-LOGICAL-c384t-f43f5c91a2e5125e5de559567c3b23258708816d5ded4d7021f33da0ff66cc873</originalsourceid><addsrcrecordid>eNqFkUtPwzAQhC0EoqXwBzigHLmk-BHHCeJSVeUhVeICZyu1N8VVGhfbKSq_HkdtOcJpV6NvR6sZhK4JHhNM8rvVOGxXzZhimkUhJ4KeoCHhjKa0FOQ07rjEKaaMDdCF9yuMcZll9BwNWFHiiGRDtJxtq6argmmXiWl915hW77yDZddE0bZRTD6s8-Dvk0kSvmzqA2yOaBpsA65qFSQBfEg63_tsrFOmheTbtOpIXqKzumo8XB3mCL0_zt6mz-n89ellOpmnihVZSOuM1VyVpKLACeXANXBe8lwotqCM8kLgoiC5jrrOtMCU1IzpCtd1nitVCDZCt3vfjbOfXfxJro1X0DRVC7bzksV8BBMlx_-jWJD4RMmLiNI9qpz1MZ1abpxZV24nCZZ9F3Il-y5k34XcdxGPbg7-3WIN-vfkGH4EHvYAxEC2Bpz0ykAMUxsHKkhtzV_-P5LAm8k</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3071512958</pqid></control><display><type>article</type><title>Evaluating insulindysregulation in horses: A two-step insulin-tolerance test using porcine zinc insulin</title><source>Elsevier ScienceDirect Journals</source><creator>Grob, A.J. ; Delarocque, J. ; Feige, K. ; Warnken, T.</creator><creatorcontrib>Grob, A.J. ; Delarocque, J. ; Feige, K. ; Warnken, T.</creatorcontrib><description>In insulin dysregulation, hyperinsulinemia (HI) can be accompanied by peripheral insulin resistance (IR) in horses, which can be diagnosed with an insulin-tolerance test (ITT). The administration of 0.1 IU/kg body weight of recombinant regular human insulin (RHI) should elicit a 50 % reduction of the initial blood glucose concentration at 30 min after insulin administration in insulin sensitive horses. Compared to RHI, porcine zinc insulin (PZI) is veterinary-approved and therefore easier accessible for many practitioners. The aim of this study was to compare the insulin and glucose dynamics during a standard ITT with RHI to an ITT performed with PZI. Twelve Icelandic horses were subjected to an ITT with RHI (ITT-RHI) and with PZI (ITT-PZI) at same dosages in a randomised crossover design. The insulin and glucose dynamics that resulted from these tests were compared, and the consistency of classification into insulin-sensitive and IR categories was evaluated. No complications were observed with the use of either RHI or PZI in ITT. A good correlation of the test results was observed (r = 0.88; P &lt; 0.001). The blood glucose concentrations and the percentage reduction in glucose concentration did not differ significantly between the two tests (P = 0.053), but four out of twelve horses were classified as IR in the ITT-RHI whereas with the ITT-PZI seven out of twelve horses were classified as IR with the 50 % glucose reduction from baseline. Based on the Youden index, when using the ITT-PZI, an adjusted cut-off value for blood glucose reduction of 40 % at 30 min resulted in better test performance. With consideration for the seemingly weaker effect of PZI and the adjusted cut-off value, PZI can be an appropriate substitute to RHI in an ITT. •Zinc insulin (PZI) could be used in an insulin-response test (IRT) in horses.•PZI used intravenously seemed less effective compared to regular insulin.•A 40 % blood glucose reduction was indicative of insulin resistance in IRT with PZI.</description><identifier>ISSN: 1090-0233</identifier><identifier>ISSN: 1532-2971</identifier><identifier>EISSN: 1532-2971</identifier><identifier>DOI: 10.1016/j.tvjl.2024.106172</identifier><identifier>PMID: 38909714</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>blood glucose ; body weight ; cross-over studies ; EMS ; glucose ; humans ; hyperinsulinemia ; insulin ; Insulin dysregulation ; Insulin resistance ; Insulin-tolerance test ; Porcine-zinc-insulin ; swine ; zinc</subject><ispartof>The veterinary journal (1997), 2024-08, Vol.306, p.106172, Article 106172</ispartof><rights>2024 The Authors</rights><rights>Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c384t-f43f5c91a2e5125e5de559567c3b23258708816d5ded4d7021f33da0ff66cc873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1090023324001114$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38909714$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grob, A.J.</creatorcontrib><creatorcontrib>Delarocque, J.</creatorcontrib><creatorcontrib>Feige, K.</creatorcontrib><creatorcontrib>Warnken, T.</creatorcontrib><title>Evaluating insulindysregulation in horses: A two-step insulin-tolerance test using porcine zinc insulin</title><title>The veterinary journal (1997)</title><addtitle>Vet J</addtitle><description>In insulin dysregulation, hyperinsulinemia (HI) can be accompanied by peripheral insulin resistance (IR) in horses, which can be diagnosed with an insulin-tolerance test (ITT). The administration of 0.1 IU/kg body weight of recombinant regular human insulin (RHI) should elicit a 50 % reduction of the initial blood glucose concentration at 30 min after insulin administration in insulin sensitive horses. Compared to RHI, porcine zinc insulin (PZI) is veterinary-approved and therefore easier accessible for many practitioners. The aim of this study was to compare the insulin and glucose dynamics during a standard ITT with RHI to an ITT performed with PZI. Twelve Icelandic horses were subjected to an ITT with RHI (ITT-RHI) and with PZI (ITT-PZI) at same dosages in a randomised crossover design. The insulin and glucose dynamics that resulted from these tests were compared, and the consistency of classification into insulin-sensitive and IR categories was evaluated. No complications were observed with the use of either RHI or PZI in ITT. A good correlation of the test results was observed (r = 0.88; P &lt; 0.001). The blood glucose concentrations and the percentage reduction in glucose concentration did not differ significantly between the two tests (P = 0.053), but four out of twelve horses were classified as IR in the ITT-RHI whereas with the ITT-PZI seven out of twelve horses were classified as IR with the 50 % glucose reduction from baseline. Based on the Youden index, when using the ITT-PZI, an adjusted cut-off value for blood glucose reduction of 40 % at 30 min resulted in better test performance. With consideration for the seemingly weaker effect of PZI and the adjusted cut-off value, PZI can be an appropriate substitute to RHI in an ITT. •Zinc insulin (PZI) could be used in an insulin-response test (IRT) in horses.•PZI used intravenously seemed less effective compared to regular insulin.•A 40 % blood glucose reduction was indicative of insulin resistance in IRT with PZI.</description><subject>blood glucose</subject><subject>body weight</subject><subject>cross-over studies</subject><subject>EMS</subject><subject>glucose</subject><subject>humans</subject><subject>hyperinsulinemia</subject><subject>insulin</subject><subject>Insulin dysregulation</subject><subject>Insulin resistance</subject><subject>Insulin-tolerance test</subject><subject>Porcine-zinc-insulin</subject><subject>swine</subject><subject>zinc</subject><issn>1090-0233</issn><issn>1532-2971</issn><issn>1532-2971</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkUtPwzAQhC0EoqXwBzigHLmk-BHHCeJSVeUhVeICZyu1N8VVGhfbKSq_HkdtOcJpV6NvR6sZhK4JHhNM8rvVOGxXzZhimkUhJ4KeoCHhjKa0FOQ07rjEKaaMDdCF9yuMcZll9BwNWFHiiGRDtJxtq6argmmXiWl915hW77yDZddE0bZRTD6s8-Dvk0kSvmzqA2yOaBpsA65qFSQBfEg63_tsrFOmheTbtOpIXqKzumo8XB3mCL0_zt6mz-n89ellOpmnihVZSOuM1VyVpKLACeXANXBe8lwotqCM8kLgoiC5jrrOtMCU1IzpCtd1nitVCDZCt3vfjbOfXfxJro1X0DRVC7bzksV8BBMlx_-jWJD4RMmLiNI9qpz1MZ1abpxZV24nCZZ9F3Il-y5k34XcdxGPbg7-3WIN-vfkGH4EHvYAxEC2Bpz0ykAMUxsHKkhtzV_-P5LAm8k</recordid><startdate>20240801</startdate><enddate>20240801</enddate><creator>Grob, A.J.</creator><creator>Delarocque, J.</creator><creator>Feige, K.</creator><creator>Warnken, T.</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240801</creationdate><title>Evaluating insulindysregulation in horses: A two-step insulin-tolerance test using porcine zinc insulin</title><author>Grob, A.J. ; Delarocque, J. ; Feige, K. ; Warnken, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-f43f5c91a2e5125e5de559567c3b23258708816d5ded4d7021f33da0ff66cc873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>blood glucose</topic><topic>body weight</topic><topic>cross-over studies</topic><topic>EMS</topic><topic>glucose</topic><topic>humans</topic><topic>hyperinsulinemia</topic><topic>insulin</topic><topic>Insulin dysregulation</topic><topic>Insulin resistance</topic><topic>Insulin-tolerance test</topic><topic>Porcine-zinc-insulin</topic><topic>swine</topic><topic>zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grob, A.J.</creatorcontrib><creatorcontrib>Delarocque, J.</creatorcontrib><creatorcontrib>Feige, K.</creatorcontrib><creatorcontrib>Warnken, T.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>The veterinary journal (1997)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grob, A.J.</au><au>Delarocque, J.</au><au>Feige, K.</au><au>Warnken, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluating insulindysregulation in horses: A two-step insulin-tolerance test using porcine zinc insulin</atitle><jtitle>The veterinary journal (1997)</jtitle><addtitle>Vet J</addtitle><date>2024-08-01</date><risdate>2024</risdate><volume>306</volume><spage>106172</spage><pages>106172-</pages><artnum>106172</artnum><issn>1090-0233</issn><issn>1532-2971</issn><eissn>1532-2971</eissn><abstract>In insulin dysregulation, hyperinsulinemia (HI) can be accompanied by peripheral insulin resistance (IR) in horses, which can be diagnosed with an insulin-tolerance test (ITT). The administration of 0.1 IU/kg body weight of recombinant regular human insulin (RHI) should elicit a 50 % reduction of the initial blood glucose concentration at 30 min after insulin administration in insulin sensitive horses. Compared to RHI, porcine zinc insulin (PZI) is veterinary-approved and therefore easier accessible for many practitioners. The aim of this study was to compare the insulin and glucose dynamics during a standard ITT with RHI to an ITT performed with PZI. Twelve Icelandic horses were subjected to an ITT with RHI (ITT-RHI) and with PZI (ITT-PZI) at same dosages in a randomised crossover design. The insulin and glucose dynamics that resulted from these tests were compared, and the consistency of classification into insulin-sensitive and IR categories was evaluated. No complications were observed with the use of either RHI or PZI in ITT. A good correlation of the test results was observed (r = 0.88; P &lt; 0.001). The blood glucose concentrations and the percentage reduction in glucose concentration did not differ significantly between the two tests (P = 0.053), but four out of twelve horses were classified as IR in the ITT-RHI whereas with the ITT-PZI seven out of twelve horses were classified as IR with the 50 % glucose reduction from baseline. Based on the Youden index, when using the ITT-PZI, an adjusted cut-off value for blood glucose reduction of 40 % at 30 min resulted in better test performance. With consideration for the seemingly weaker effect of PZI and the adjusted cut-off value, PZI can be an appropriate substitute to RHI in an ITT. •Zinc insulin (PZI) could be used in an insulin-response test (IRT) in horses.•PZI used intravenously seemed less effective compared to regular insulin.•A 40 % blood glucose reduction was indicative of insulin resistance in IRT with PZI.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>38909714</pmid><doi>10.1016/j.tvjl.2024.106172</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1090-0233
ispartof The veterinary journal (1997), 2024-08, Vol.306, p.106172, Article 106172
issn 1090-0233
1532-2971
1532-2971
language eng
recordid cdi_proquest_miscellaneous_3153737950
source Elsevier ScienceDirect Journals
subjects blood glucose
body weight
cross-over studies
EMS
glucose
humans
hyperinsulinemia
insulin
Insulin dysregulation
Insulin resistance
Insulin-tolerance test
Porcine-zinc-insulin
swine
zinc
title Evaluating insulindysregulation in horses: A two-step insulin-tolerance test using porcine zinc insulin
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T05%3A20%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluating%20insulindysregulation%20in%20horses:%20A%20two-step%20insulin-tolerance%20test%20using%20porcine%20zinc%20insulin&rft.jtitle=The%20veterinary%20journal%20(1997)&rft.au=Grob,%20A.J.&rft.date=2024-08-01&rft.volume=306&rft.spage=106172&rft.pages=106172-&rft.artnum=106172&rft.issn=1090-0233&rft.eissn=1532-2971&rft_id=info:doi/10.1016/j.tvjl.2024.106172&rft_dat=%3Cproquest_cross%3E3071512958%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3071512958&rft_id=info:pmid/38909714&rft_els_id=S1090023324001114&rfr_iscdi=true