Dendrobine Ameliorates Glucocorticoid-Induced Osteoporosis by Promoting Osteogenesis through JNK/p38 MAPK Pathway Activation and GR Nuclear Translocation Inhibition
Glucocorticoid-induced osteoporosis (GIOP) is the common reason for secondary osteoporosis. Dendrobine (DEN) is the major biologically active component of Dendrobium officinale with anti-inflammatory and antiaging properties. Whether DEN could alleviate osteogenic inhibition in GIOP rats is still un...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2024-07, Vol.72 (30), p.16739-16748 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Li, Xufeng Gao, Chenyi Zhou, Ke Gan, Kaifeng Ye, Tianjie Zhao, Jun Li, Jin Ma, Chi |
| description | Glucocorticoid-induced osteoporosis (GIOP) is the common reason for secondary osteoporosis. Dendrobine (DEN) is the major biologically active component of Dendrobium officinale with anti-inflammatory and antiaging properties. Whether DEN could alleviate osteogenic inhibition in GIOP rats is still unknown. The influence on osteogenic function caused by DEN on dexamethasone-treated bone marrow mesenchymal stem cells and rats was observed. The in vitro results showed that DEN reversed the inhibition of osteogenic differentiation by dexamethasone. Moreover, DEN supplementation attenuated dexamethasone-induced bone loss in vivo. DEN activated JNK and p38 MAPK pathways and restrained GR nuclear translocation, which could be prevented by the JNK (SP600125) or p38 (SB203580) pathway inhibitor. This study verified that DEN alleviated dexamethasone-induced nuclear translocation of GR, and inhibition of osteogenesis via JNK and p38 pathways, laying the foundation for DEN as a therapeutic agent for GIOP. |
| doi_str_mv | 10.1021/acs.jafc.4c02798 |
| format | Article |
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Dendrobine (DEN) is the major biologically active component of Dendrobium officinale with anti-inflammatory and antiaging properties. Whether DEN could alleviate osteogenic inhibition in GIOP rats is still unknown. The influence on osteogenic function caused by DEN on dexamethasone-treated bone marrow mesenchymal stem cells and rats was observed. The in vitro results showed that DEN reversed the inhibition of osteogenic differentiation by dexamethasone. Moreover, DEN supplementation attenuated dexamethasone-induced bone loss in vivo. DEN activated JNK and p38 MAPK pathways and restrained GR nuclear translocation, which could be prevented by the JNK (SP600125) or p38 (SB203580) pathway inhibitor. This study verified that DEN alleviated dexamethasone-induced nuclear translocation of GR, and inhibition of osteogenesis via JNK and p38 pathways, laying the foundation for DEN as a therapeutic agent for GIOP.</description><identifier>ISSN: 0021-8561</identifier><identifier>ISSN: 1520-5118</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.4c02798</identifier><identifier>PMID: 39033544</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Bioactive Constituents, Metabolites, and Functions ; bone formation ; bone marrow ; bone resorption ; Cell Differentiation - drug effects ; Dendrobium officinale ; dexamethasone ; Dexamethasone - adverse effects ; food chemistry ; Glucocorticoids - adverse effects ; Humans ; JNK Mitogen-Activated Protein Kinases - genetics ; JNK Mitogen-Activated Protein Kinases - metabolism ; Male ; MAP Kinase Signaling System - drug effects ; Mesenchymal Stem Cells - drug effects ; Mesenchymal Stem Cells - metabolism ; mitogen-activated protein kinase ; Osteogenesis - drug effects ; osteoporosis ; Osteoporosis - chemically induced ; Osteoporosis - drug therapy ; Osteoporosis - metabolism ; Osteoporosis - prevention & control ; p38 Mitogen-Activated Protein Kinases - genetics ; p38 Mitogen-Activated Protein Kinases - metabolism ; Plant Extracts - pharmacology ; Rats ; Rats, Sprague-Dawley ; Receptors, Glucocorticoid - genetics ; Receptors, Glucocorticoid - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2024-07, Vol.72 (30), p.16739-16748</ispartof><rights>2024 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a322t-5dd8068704f8cf9a783ff9dc44aae8ce6ceb443aa0aa1c85e93fdc1d4f9160d23</cites><orcidid>0000-0003-3491-8891</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.4c02798$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.4c02798$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39033544$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xufeng</creatorcontrib><creatorcontrib>Gao, Chenyi</creatorcontrib><creatorcontrib>Zhou, Ke</creatorcontrib><creatorcontrib>Gan, Kaifeng</creatorcontrib><creatorcontrib>Ye, Tianjie</creatorcontrib><creatorcontrib>Zhao, Jun</creatorcontrib><creatorcontrib>Li, Jin</creatorcontrib><creatorcontrib>Ma, Chi</creatorcontrib><title>Dendrobine Ameliorates Glucocorticoid-Induced Osteoporosis by Promoting Osteogenesis through JNK/p38 MAPK Pathway Activation and GR Nuclear Translocation Inhibition</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Glucocorticoid-induced osteoporosis (GIOP) is the common reason for secondary osteoporosis. Dendrobine (DEN) is the major biologically active component of Dendrobium officinale with anti-inflammatory and antiaging properties. Whether DEN could alleviate osteogenic inhibition in GIOP rats is still unknown. The influence on osteogenic function caused by DEN on dexamethasone-treated bone marrow mesenchymal stem cells and rats was observed. The in vitro results showed that DEN reversed the inhibition of osteogenic differentiation by dexamethasone. Moreover, DEN supplementation attenuated dexamethasone-induced bone loss in vivo. DEN activated JNK and p38 MAPK pathways and restrained GR nuclear translocation, which could be prevented by the JNK (SP600125) or p38 (SB203580) pathway inhibitor. This study verified that DEN alleviated dexamethasone-induced nuclear translocation of GR, and inhibition of osteogenesis via JNK and p38 pathways, laying the foundation for DEN as a therapeutic agent for GIOP.</description><subject>Animals</subject><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>bone formation</subject><subject>bone marrow</subject><subject>bone resorption</subject><subject>Cell Differentiation - drug effects</subject><subject>Dendrobium officinale</subject><subject>dexamethasone</subject><subject>Dexamethasone - adverse effects</subject><subject>food chemistry</subject><subject>Glucocorticoids - adverse effects</subject><subject>Humans</subject><subject>JNK Mitogen-Activated Protein Kinases - genetics</subject><subject>JNK Mitogen-Activated Protein Kinases - metabolism</subject><subject>Male</subject><subject>MAP Kinase Signaling System - drug effects</subject><subject>Mesenchymal Stem Cells - drug effects</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>mitogen-activated protein kinase</subject><subject>Osteogenesis - drug effects</subject><subject>osteoporosis</subject><subject>Osteoporosis - chemically induced</subject><subject>Osteoporosis - drug therapy</subject><subject>Osteoporosis - metabolism</subject><subject>Osteoporosis - prevention & control</subject><subject>p38 Mitogen-Activated Protein Kinases - genetics</subject><subject>p38 Mitogen-Activated Protein Kinases - metabolism</subject><subject>Plant Extracts - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Glucocorticoid - genetics</subject><subject>Receptors, Glucocorticoid - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv0zAYhi0EYmVw54R85EA6O3Ya51gNVsrGVqFxjr7YX1pPid3ZDlP_Dz-UlBRuiJMtvc_7Hr6HkLeczTnL-QXoOH-AVs-lZnlZqWdkxoucZQXn6jmZsZHJVLHgZ-RVjA-MMVWU7CU5ExUTopByRn5-RGeCb6xDuuyxsz5AwkhX3aC99iFZ7a3J1s4MGg29iwn93gcfbaTNgW6C732ybjslW3R4TNIu-GG7o19ury_2QtGvy8013UDaPcGBLnWyPyBZ7yg4Q1ff6O2gO4RA7wO42Hk9hWu3s409fl-TFy10Ed-c3nPy_erT_eXn7OZutb5c3mQg8jxlhTGKLVTJZKt0W0GpRNtWRksJgErjQmMjpQBgAFyrAivRGs2NbCu-YCYX5-T9tLsP_nHAmOreRo1dBw79EGvBC1EKWXH5f5QpkY-rJR9RNqF6PFsM2Nb7YHsIh5qz-qixHjXWR431SeNYeXdaH5oezd_CH28j8GECflf9ENx4l3_v_QKAqqyo</recordid><startdate>20240731</startdate><enddate>20240731</enddate><creator>Li, Xufeng</creator><creator>Gao, Chenyi</creator><creator>Zhou, Ke</creator><creator>Gan, Kaifeng</creator><creator>Ye, Tianjie</creator><creator>Zhao, Jun</creator><creator>Li, Jin</creator><creator>Ma, Chi</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-3491-8891</orcidid></search><sort><creationdate>20240731</creationdate><title>Dendrobine Ameliorates Glucocorticoid-Induced Osteoporosis by Promoting Osteogenesis through JNK/p38 MAPK Pathway Activation and GR Nuclear Translocation Inhibition</title><author>Li, Xufeng ; Gao, Chenyi ; Zhou, Ke ; Gan, Kaifeng ; Ye, Tianjie ; Zhao, Jun ; Li, Jin ; Ma, Chi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a322t-5dd8068704f8cf9a783ff9dc44aae8ce6ceb443aa0aa1c85e93fdc1d4f9160d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>bone formation</topic><topic>bone marrow</topic><topic>bone resorption</topic><topic>Cell Differentiation - drug effects</topic><topic>Dendrobium officinale</topic><topic>dexamethasone</topic><topic>Dexamethasone - adverse effects</topic><topic>food chemistry</topic><topic>Glucocorticoids - adverse effects</topic><topic>Humans</topic><topic>JNK Mitogen-Activated Protein Kinases - genetics</topic><topic>JNK Mitogen-Activated Protein Kinases - metabolism</topic><topic>Male</topic><topic>MAP Kinase Signaling System - drug effects</topic><topic>Mesenchymal Stem Cells - drug effects</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>mitogen-activated protein kinase</topic><topic>Osteogenesis - drug effects</topic><topic>osteoporosis</topic><topic>Osteoporosis - chemically induced</topic><topic>Osteoporosis - drug therapy</topic><topic>Osteoporosis - metabolism</topic><topic>Osteoporosis - prevention & control</topic><topic>p38 Mitogen-Activated Protein Kinases - genetics</topic><topic>p38 Mitogen-Activated Protein Kinases - metabolism</topic><topic>Plant Extracts - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Glucocorticoid - genetics</topic><topic>Receptors, Glucocorticoid - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xufeng</creatorcontrib><creatorcontrib>Gao, Chenyi</creatorcontrib><creatorcontrib>Zhou, Ke</creatorcontrib><creatorcontrib>Gan, Kaifeng</creatorcontrib><creatorcontrib>Ye, Tianjie</creatorcontrib><creatorcontrib>Zhao, Jun</creatorcontrib><creatorcontrib>Li, Jin</creatorcontrib><creatorcontrib>Ma, Chi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xufeng</au><au>Gao, Chenyi</au><au>Zhou, Ke</au><au>Gan, Kaifeng</au><au>Ye, Tianjie</au><au>Zhao, Jun</au><au>Li, Jin</au><au>Ma, Chi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dendrobine Ameliorates Glucocorticoid-Induced Osteoporosis by Promoting Osteogenesis through JNK/p38 MAPK Pathway Activation and GR Nuclear Translocation Inhibition</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2024-07-31</date><risdate>2024</risdate><volume>72</volume><issue>30</issue><spage>16739</spage><epage>16748</epage><pages>16739-16748</pages><issn>0021-8561</issn><issn>1520-5118</issn><eissn>1520-5118</eissn><abstract>Glucocorticoid-induced osteoporosis (GIOP) is the common reason for secondary osteoporosis. Dendrobine (DEN) is the major biologically active component of Dendrobium officinale with anti-inflammatory and antiaging properties. Whether DEN could alleviate osteogenic inhibition in GIOP rats is still unknown. The influence on osteogenic function caused by DEN on dexamethasone-treated bone marrow mesenchymal stem cells and rats was observed. The in vitro results showed that DEN reversed the inhibition of osteogenic differentiation by dexamethasone. Moreover, DEN supplementation attenuated dexamethasone-induced bone loss in vivo. DEN activated JNK and p38 MAPK pathways and restrained GR nuclear translocation, which could be prevented by the JNK (SP600125) or p38 (SB203580) pathway inhibitor. This study verified that DEN alleviated dexamethasone-induced nuclear translocation of GR, and inhibition of osteogenesis via JNK and p38 pathways, laying the foundation for DEN as a therapeutic agent for GIOP.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>39033544</pmid><doi>10.1021/acs.jafc.4c02798</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-3491-8891</orcidid></addata></record> |
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| subjects | Animals Bioactive Constituents, Metabolites, and Functions bone formation bone marrow bone resorption Cell Differentiation - drug effects Dendrobium officinale dexamethasone Dexamethasone - adverse effects food chemistry Glucocorticoids - adverse effects Humans JNK Mitogen-Activated Protein Kinases - genetics JNK Mitogen-Activated Protein Kinases - metabolism Male MAP Kinase Signaling System - drug effects Mesenchymal Stem Cells - drug effects Mesenchymal Stem Cells - metabolism mitogen-activated protein kinase Osteogenesis - drug effects osteoporosis Osteoporosis - chemically induced Osteoporosis - drug therapy Osteoporosis - metabolism Osteoporosis - prevention & control p38 Mitogen-Activated Protein Kinases - genetics p38 Mitogen-Activated Protein Kinases - metabolism Plant Extracts - pharmacology Rats Rats, Sprague-Dawley Receptors, Glucocorticoid - genetics Receptors, Glucocorticoid - metabolism |
| title | Dendrobine Ameliorates Glucocorticoid-Induced Osteoporosis by Promoting Osteogenesis through JNK/p38 MAPK Pathway Activation and GR Nuclear Translocation Inhibition |
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