Examining the stability of viral RNA and DNA in wastewater: Effects of storage time, temperature, and freeze-thaw cycles

•S gene of SARS-Cov-2 is more sensitive to freeze-thaw than other regions tested.•Phi6 is a stable surrogate for SARS-CoV-2 under different storage conditions.•Freeze-thawing raw wastewater negatively impacts next-generation sequencing metrics.•Historical (12–16 months) wastewater samples stored at...

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Veröffentlicht in:	Water research (Oxford) 2024-08, Vol.259, p.121879, Article 121879
Hauptverfasser:	Williams, Rachel C., Perry, William B., Lambert-Slosarska, Kathryn, Futcher, Ben, Pellett, Cameron, Richardson-O'Neill, India, Paterson, Steve, Grimsley, Jasmine M.S., Wade, Matthew J., Weightman, Andrew J., Farkas, Kata, Jones, Davey L.
Format:	Artikel
Sprache:	eng
Schlagworte:	antibiotic resistance bacteriophages Biobank Biorepository COVID-19 infection DNA electrophoresis epidemiology Freeze-thaw freeze-thaw cycles genes humans Nucleic acid preservation nucleocapsid public health quantitative polymerase chain reaction reverse transcriptase polymerase chain reaction RNA Sample storage Severe acute respiratory syndrome coronavirus 2 storage time temperature wastewater water WBE
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