Expression of CD44 is regulated by ELF3 in 5-FU treated colorectal cancer cells
•The expression of CD44, including some other CSC markers were all increased after 5-FU treatment of CRC cells. The stemness properties of survived CRC cells were also observed to be enhanced.•RNA-seq analysis revealed that ELF3 was increased along with CD44 after 5-FU treatment in CRC cells.•The ex...
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| Veröffentlicht in: | Gene 2024-01, Vol.892, p.147896-147896, Article 147896 |
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| creator | Li, Fangzhou Qiu, Fen Fan, Xu Yu, Qingqing Liu, Shuaitong Guo, Yang Zhu, Yunhe Xi, Xueyan Du, Boyu |
| description | •The expression of CD44, including some other CSC markers were all increased after 5-FU treatment of CRC cells. The stemness properties of survived CRC cells were also observed to be enhanced.•RNA-seq analysis revealed that ELF3 was increased along with CD44 after 5-FU treatment in CRC cells.•The expression of ELF3 is significantly higher in CD44+ CRC cells. CD44 expression might be regulated by ELF3 and could be induced after 5-FU treatment in CRC cells.
The development of chemoresistance in colorectal cancer (CRC) cells was usually thought to be inevitable as a result of continuing exposure to chemotherapeutic drugs. The existence of cancer stem cells (CSCs) within CRC tissues was recently suggested to play importance roles for this process. In this study, in order to mimic a dose schedule used in clinic (continuous infusion), low dose of fluorouracil (IC10 of 5-FU) was used to treat CRC cells. Our results showed that the expression of CD44, including some other CSCs markers were all increased after 5-FU treatment. The stemness properties of survived CRC cells were also observed to be enhanced. RNA-seq analysis revealed that ELF3, one of the members of ETS (E26 transformation-specific) transcription activator family, was increased along with CD44 after 5-FU treatment of CRC cells. Results from dual-luciferase reporter assay revealed that the transcription of CD44 could be activated by ELF3 in CRC cells. The induced CD44 expression in 5-FU treated CRC cells could also be decreased after the expression of ELF3 was inhibited. Moreover, it could be observed that the expression of ELF3 is significantly higher in CD44+ CRC cells. Taken together, our results suggested that CD44 expression might be regulated by ELF3 and could be induced after 5-FU treatment of CRC cells. Inhibition of ELF3 might be a promising treatment method when it was used in combination with chemotherapeutics to overcome chemoresistance formation during CRC treatment in clinic. |
| doi_str_mv | 10.1016/j.gene.2023.147896 |
| format | Article |
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The development of chemoresistance in colorectal cancer (CRC) cells was usually thought to be inevitable as a result of continuing exposure to chemotherapeutic drugs. The existence of cancer stem cells (CSCs) within CRC tissues was recently suggested to play importance roles for this process. In this study, in order to mimic a dose schedule used in clinic (continuous infusion), low dose of fluorouracil (IC10 of 5-FU) was used to treat CRC cells. Our results showed that the expression of CD44, including some other CSCs markers were all increased after 5-FU treatment. The stemness properties of survived CRC cells were also observed to be enhanced. RNA-seq analysis revealed that ELF3, one of the members of ETS (E26 transformation-specific) transcription activator family, was increased along with CD44 after 5-FU treatment of CRC cells. Results from dual-luciferase reporter assay revealed that the transcription of CD44 could be activated by ELF3 in CRC cells. The induced CD44 expression in 5-FU treated CRC cells could also be decreased after the expression of ELF3 was inhibited. Moreover, it could be observed that the expression of ELF3 is significantly higher in CD44+ CRC cells. Taken together, our results suggested that CD44 expression might be regulated by ELF3 and could be induced after 5-FU treatment of CRC cells. Inhibition of ELF3 might be a promising treatment method when it was used in combination with chemotherapeutics to overcome chemoresistance formation during CRC treatment in clinic.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2023.147896</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>5-fluorouracil ; bioluminescence assay ; CD44 ; Chemotherapy ; Colorectal cancer ; colorectal neoplasms ; drug therapy ; ELF3 ; family ; fluorouracil ; genes ; sequence analysis</subject><ispartof>Gene, 2024-01, Vol.892, p.147896-147896, Article 147896</ispartof><rights>2023 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c317t-8f9146fb8711d49b7b9a2e319fba215bf9b6abb22bc29588d6c6f3806f69247b3</cites><orcidid>0000-0002-3164-9895</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378111923007370$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Li, Fangzhou</creatorcontrib><creatorcontrib>Qiu, Fen</creatorcontrib><creatorcontrib>Fan, Xu</creatorcontrib><creatorcontrib>Yu, Qingqing</creatorcontrib><creatorcontrib>Liu, Shuaitong</creatorcontrib><creatorcontrib>Guo, Yang</creatorcontrib><creatorcontrib>Zhu, Yunhe</creatorcontrib><creatorcontrib>Xi, Xueyan</creatorcontrib><creatorcontrib>Du, Boyu</creatorcontrib><title>Expression of CD44 is regulated by ELF3 in 5-FU treated colorectal cancer cells</title><title>Gene</title><description>•The expression of CD44, including some other CSC markers were all increased after 5-FU treatment of CRC cells. The stemness properties of survived CRC cells were also observed to be enhanced.•RNA-seq analysis revealed that ELF3 was increased along with CD44 after 5-FU treatment in CRC cells.•The expression of ELF3 is significantly higher in CD44+ CRC cells. CD44 expression might be regulated by ELF3 and could be induced after 5-FU treatment in CRC cells.
The development of chemoresistance in colorectal cancer (CRC) cells was usually thought to be inevitable as a result of continuing exposure to chemotherapeutic drugs. The existence of cancer stem cells (CSCs) within CRC tissues was recently suggested to play importance roles for this process. In this study, in order to mimic a dose schedule used in clinic (continuous infusion), low dose of fluorouracil (IC10 of 5-FU) was used to treat CRC cells. Our results showed that the expression of CD44, including some other CSCs markers were all increased after 5-FU treatment. The stemness properties of survived CRC cells were also observed to be enhanced. RNA-seq analysis revealed that ELF3, one of the members of ETS (E26 transformation-specific) transcription activator family, was increased along with CD44 after 5-FU treatment of CRC cells. Results from dual-luciferase reporter assay revealed that the transcription of CD44 could be activated by ELF3 in CRC cells. The induced CD44 expression in 5-FU treated CRC cells could also be decreased after the expression of ELF3 was inhibited. Moreover, it could be observed that the expression of ELF3 is significantly higher in CD44+ CRC cells. Taken together, our results suggested that CD44 expression might be regulated by ELF3 and could be induced after 5-FU treatment of CRC cells. Inhibition of ELF3 might be a promising treatment method when it was used in combination with chemotherapeutics to overcome chemoresistance formation during CRC treatment in clinic.</description><subject>5-fluorouracil</subject><subject>bioluminescence assay</subject><subject>CD44</subject><subject>Chemotherapy</subject><subject>Colorectal cancer</subject><subject>colorectal neoplasms</subject><subject>drug therapy</subject><subject>ELF3</subject><subject>family</subject><subject>fluorouracil</subject><subject>genes</subject><subject>sequence analysis</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkD1PwzAQhi0EEqXwB5g8siT4I4ltiQWVFpAqdaGzZTvnylWaFDtF8O9JCDPcctLpfU53D0K3lOSU0Op-n--ghZwRxnNaCKmqMzSjUqiMEC7P0YxwITNKqbpEVyntyVBlyWZos_w8RkgpdC3uPF48FQUOCUfYnRrTQ43tF16uVxyHFpfZaov7CD9z1zVdBNebBjvTOojYQdOka3ThTZPg5rfP0Xa1fFu8ZOvN8-vicZ05TkWfSa9oUXkrBaV1oaywyjDgVHlrGC2tV7Yy1jJmHVOllHXlKs8lqXylWCEsn6O7ae8xdu8nSL0-hDReYFroTklzWnLBylKyf6NMCsElUwUfomyKutilFMHrYwwHE780JXoUrfd6FK1H0XoSPUAPEwTDvx8Bok4uwKCkDqMgXXfhL_wbXnOEIQ</recordid><startdate>20240120</startdate><enddate>20240120</enddate><creator>Li, Fangzhou</creator><creator>Qiu, Fen</creator><creator>Fan, Xu</creator><creator>Yu, Qingqing</creator><creator>Liu, Shuaitong</creator><creator>Guo, Yang</creator><creator>Zhu, Yunhe</creator><creator>Xi, Xueyan</creator><creator>Du, Boyu</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-3164-9895</orcidid></search><sort><creationdate>20240120</creationdate><title>Expression of CD44 is regulated by ELF3 in 5-FU treated colorectal cancer cells</title><author>Li, Fangzhou ; Qiu, Fen ; Fan, Xu ; Yu, Qingqing ; Liu, Shuaitong ; Guo, Yang ; Zhu, Yunhe ; Xi, Xueyan ; Du, Boyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c317t-8f9146fb8711d49b7b9a2e319fba215bf9b6abb22bc29588d6c6f3806f69247b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>5-fluorouracil</topic><topic>bioluminescence assay</topic><topic>CD44</topic><topic>Chemotherapy</topic><topic>Colorectal cancer</topic><topic>colorectal neoplasms</topic><topic>drug therapy</topic><topic>ELF3</topic><topic>family</topic><topic>fluorouracil</topic><topic>genes</topic><topic>sequence analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Fangzhou</creatorcontrib><creatorcontrib>Qiu, Fen</creatorcontrib><creatorcontrib>Fan, Xu</creatorcontrib><creatorcontrib>Yu, Qingqing</creatorcontrib><creatorcontrib>Liu, Shuaitong</creatorcontrib><creatorcontrib>Guo, Yang</creatorcontrib><creatorcontrib>Zhu, Yunhe</creatorcontrib><creatorcontrib>Xi, Xueyan</creatorcontrib><creatorcontrib>Du, Boyu</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Fangzhou</au><au>Qiu, Fen</au><au>Fan, Xu</au><au>Yu, Qingqing</au><au>Liu, Shuaitong</au><au>Guo, Yang</au><au>Zhu, Yunhe</au><au>Xi, Xueyan</au><au>Du, Boyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of CD44 is regulated by ELF3 in 5-FU treated colorectal cancer cells</atitle><jtitle>Gene</jtitle><date>2024-01-20</date><risdate>2024</risdate><volume>892</volume><spage>147896</spage><epage>147896</epage><pages>147896-147896</pages><artnum>147896</artnum><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>•The expression of CD44, including some other CSC markers were all increased after 5-FU treatment of CRC cells. The stemness properties of survived CRC cells were also observed to be enhanced.•RNA-seq analysis revealed that ELF3 was increased along with CD44 after 5-FU treatment in CRC cells.•The expression of ELF3 is significantly higher in CD44+ CRC cells. CD44 expression might be regulated by ELF3 and could be induced after 5-FU treatment in CRC cells.
The development of chemoresistance in colorectal cancer (CRC) cells was usually thought to be inevitable as a result of continuing exposure to chemotherapeutic drugs. The existence of cancer stem cells (CSCs) within CRC tissues was recently suggested to play importance roles for this process. In this study, in order to mimic a dose schedule used in clinic (continuous infusion), low dose of fluorouracil (IC10 of 5-FU) was used to treat CRC cells. Our results showed that the expression of CD44, including some other CSCs markers were all increased after 5-FU treatment. The stemness properties of survived CRC cells were also observed to be enhanced. RNA-seq analysis revealed that ELF3, one of the members of ETS (E26 transformation-specific) transcription activator family, was increased along with CD44 after 5-FU treatment of CRC cells. Results from dual-luciferase reporter assay revealed that the transcription of CD44 could be activated by ELF3 in CRC cells. The induced CD44 expression in 5-FU treated CRC cells could also be decreased after the expression of ELF3 was inhibited. Moreover, it could be observed that the expression of ELF3 is significantly higher in CD44+ CRC cells. Taken together, our results suggested that CD44 expression might be regulated by ELF3 and could be induced after 5-FU treatment of CRC cells. Inhibition of ELF3 might be a promising treatment method when it was used in combination with chemotherapeutics to overcome chemoresistance formation during CRC treatment in clinic.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.gene.2023.147896</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-3164-9895</orcidid></addata></record> |
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| subjects | 5-fluorouracil bioluminescence assay CD44 Chemotherapy Colorectal cancer colorectal neoplasms drug therapy ELF3 family fluorouracil genes sequence analysis |
| title | Expression of CD44 is regulated by ELF3 in 5-FU treated colorectal cancer cells |
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