Loop-mediated isothermal amplification (LAMP) assay for early on-site detection of Group B Streptococcus infection in neonatal sepsis blood sample
Background Neonatal sepsis, often attributed to Group B Streptococcus (GBS) infection, poses a critical health risk to infants, demanding rapid and accurate diagnostic approaches. Existing diagnostic approaches are dependent on traditional culture methods, a process that requires substantial time an...
Gespeichert in:
| Veröffentlicht in: | Molecular biology reports 2024-12, Vol.51 (1), p.811-811, Article 811 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 811 |
|---|---|
| container_issue | 1 |
| container_start_page | 811 |
| container_title | Molecular biology reports |
| container_volume | 51 |
| creator | Banerjee, Shukla K., Mithun H. Shastry, Rajesh P. |
| description | Background
Neonatal sepsis, often attributed to Group B
Streptococcus
(GBS) infection, poses a critical health risk to infants, demanding rapid and accurate diagnostic approaches. Existing diagnostic approaches are dependent on traditional culture methods, a process that requires substantial time and has the potential to delay crucial therapeutic assessments.
Methods
This study introduces an innovative Loop-Mediated Isothermal Amplification (LAMP) assay for the early on-site detection of GBS infection from neonatal sepsis blood samples. To develop a LAMP assay, the primers are designed for the selective targeting of a highly conserved segment within the
cfb
gene encoding the CAMP factor in
Streptococcus agalactiae
ensuring high specificity.
Results
Rigorous optimization of reaction conditions, including temperature and incubation time, enhances the efficiency of the LAMP assay, enabling rapid and reliable GBS detection within a short timeframe. The diagnostic efficacy of the LAMP assay was evaluated using spiked blood samples by eliminating the DNA extraction step. The simplified colorimetric LAMP assay has the capability to detect
S. agalactiae
in a neonatal blood sample containing 2 CFU/mL during sepsis. Additionally, the LAMP assay effectively detected
S. agalactiae
in both the standard and spiked blood samples, with no detectable interference with blood.
Conclusion
This optimised LAMP assay emerges as a promising tool for early GBS detection, offering a rapid and accurate on-site solution that has the potential to inform timely interventions and improve outcomes in neonatal sepsis cases. |
| doi_str_mv | 10.1007/s11033-024-09705-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153717734</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3079955663</sourcerecordid><originalsourceid>FETCH-LOGICAL-c289t-fca3111656353dd907b396d99a9cddd3103e9668682db6c229055fa97ccdb4c33</originalsourceid><addsrcrecordid>eNqFkcGO1SAUhonRONfRF3BhSNyMC_RQCpTlzERHk2s0UdeEAlUmbalAF_c1fGK506smLnTFgu_8P4cPoacUXlIA-SpTCowRaFoCSgIncA_tKJeMtEp299EOGFDSdpyeoUc53wJASyV_iM6YAmiAdTv0Yx_jQibvgine4ZBj-ebTZEZspmUMQ7CmhDjji_3l-48vsMnZHPAQE_YmjQccZ5JD8dj54u0dGAd8k-K64Cv8qSS_lGijtWvGYR5OSJjx7ONsSm3Jfskh436M0eF87PSP0YPBjNk_OZ3n6Mub15-v35L9h5t315d7YptOFTJYwyilggvGmXMKZM-UcEoZZZ1zrP6NV0J0omtcL2zTKOB8MEpa6_rWMnaOLrbcJcXvq89FTyFbP46mvm7NmlHOJJWStf9HQSrFuRDH1Od_obdxTXNdZKNYKzpVqWajbIo5Jz_oJYXJpIOmoI9y9SZXV7n6Tq6GOvTsFL321djvkV82K8A2INer-atPf7r_EfsTICav_Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3079934689</pqid></control><display><type>article</type><title>Loop-mediated isothermal amplification (LAMP) assay for early on-site detection of Group B Streptococcus infection in neonatal sepsis blood sample</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Banerjee, Shukla ; K., Mithun H. ; Shastry, Rajesh P.</creator><creatorcontrib>Banerjee, Shukla ; K., Mithun H. ; Shastry, Rajesh P.</creatorcontrib><description>Background
Neonatal sepsis, often attributed to Group B
Streptococcus
(GBS) infection, poses a critical health risk to infants, demanding rapid and accurate diagnostic approaches. Existing diagnostic approaches are dependent on traditional culture methods, a process that requires substantial time and has the potential to delay crucial therapeutic assessments.
Methods
This study introduces an innovative Loop-Mediated Isothermal Amplification (LAMP) assay for the early on-site detection of GBS infection from neonatal sepsis blood samples. To develop a LAMP assay, the primers are designed for the selective targeting of a highly conserved segment within the
cfb
gene encoding the CAMP factor in
Streptococcus agalactiae
ensuring high specificity.
Results
Rigorous optimization of reaction conditions, including temperature and incubation time, enhances the efficiency of the LAMP assay, enabling rapid and reliable GBS detection within a short timeframe. The diagnostic efficacy of the LAMP assay was evaluated using spiked blood samples by eliminating the DNA extraction step. The simplified colorimetric LAMP assay has the capability to detect
S. agalactiae
in a neonatal blood sample containing 2 CFU/mL during sepsis. Additionally, the LAMP assay effectively detected
S. agalactiae
in both the standard and spiked blood samples, with no detectable interference with blood.
Conclusion
This optimised LAMP assay emerges as a promising tool for early GBS detection, offering a rapid and accurate on-site solution that has the potential to inform timely interventions and improve outcomes in neonatal sepsis cases.</description><identifier>ISSN: 0301-4851</identifier><identifier>ISSN: 1573-4978</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-024-09705-0</identifier><identifier>PMID: 39002038</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Bacterial Proteins - genetics ; Biomedical and Life Sciences ; blood ; blood sampling ; Colorimetry ; DNA ; DNA, Bacterial - blood ; DNA, Bacterial - genetics ; Gene amplification ; genes ; Histology ; Humans ; Infant, Newborn ; Life Sciences ; loop-mediated isothermal amplification ; Medical innovations ; Molecular Diagnostic Techniques - methods ; Morphology ; Neonatal Sepsis - blood ; Neonatal Sepsis - diagnosis ; Neonatal Sepsis - microbiology ; Neonates ; Nucleic Acid Amplification Techniques - methods ; Onsite ; Original Article ; risk ; Sensitivity and Specificity ; Sepsis ; Streptococcal Infections - blood ; Streptococcal Infections - diagnosis ; Streptococcal Infections - microbiology ; Streptococcus ; Streptococcus agalactiae ; Streptococcus agalactiae - genetics ; Streptococcus agalactiae - isolation & purification ; Streptococcus infections ; temperature ; Temperature requirements ; therapeutics</subject><ispartof>Molecular biology reports, 2024-12, Vol.51 (1), p.811-811, Article 811</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c289t-fca3111656353dd907b396d99a9cddd3103e9668682db6c229055fa97ccdb4c33</cites><orcidid>0000-0001-8627-9759</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-024-09705-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-024-09705-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39002038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Banerjee, Shukla</creatorcontrib><creatorcontrib>K., Mithun H.</creatorcontrib><creatorcontrib>Shastry, Rajesh P.</creatorcontrib><title>Loop-mediated isothermal amplification (LAMP) assay for early on-site detection of Group B Streptococcus infection in neonatal sepsis blood sample</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
Neonatal sepsis, often attributed to Group B
Streptococcus
(GBS) infection, poses a critical health risk to infants, demanding rapid and accurate diagnostic approaches. Existing diagnostic approaches are dependent on traditional culture methods, a process that requires substantial time and has the potential to delay crucial therapeutic assessments.
Methods
This study introduces an innovative Loop-Mediated Isothermal Amplification (LAMP) assay for the early on-site detection of GBS infection from neonatal sepsis blood samples. To develop a LAMP assay, the primers are designed for the selective targeting of a highly conserved segment within the
cfb
gene encoding the CAMP factor in
Streptococcus agalactiae
ensuring high specificity.
Results
Rigorous optimization of reaction conditions, including temperature and incubation time, enhances the efficiency of the LAMP assay, enabling rapid and reliable GBS detection within a short timeframe. The diagnostic efficacy of the LAMP assay was evaluated using spiked blood samples by eliminating the DNA extraction step. The simplified colorimetric LAMP assay has the capability to detect
S. agalactiae
in a neonatal blood sample containing 2 CFU/mL during sepsis. Additionally, the LAMP assay effectively detected
S. agalactiae
in both the standard and spiked blood samples, with no detectable interference with blood.
Conclusion
This optimised LAMP assay emerges as a promising tool for early GBS detection, offering a rapid and accurate on-site solution that has the potential to inform timely interventions and improve outcomes in neonatal sepsis cases.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>blood</subject><subject>blood sampling</subject><subject>Colorimetry</subject><subject>DNA</subject><subject>DNA, Bacterial - blood</subject><subject>DNA, Bacterial - genetics</subject><subject>Gene amplification</subject><subject>genes</subject><subject>Histology</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Life Sciences</subject><subject>loop-mediated isothermal amplification</subject><subject>Medical innovations</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Morphology</subject><subject>Neonatal Sepsis - blood</subject><subject>Neonatal Sepsis - diagnosis</subject><subject>Neonatal Sepsis - microbiology</subject><subject>Neonates</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>Onsite</subject><subject>Original Article</subject><subject>risk</subject><subject>Sensitivity and Specificity</subject><subject>Sepsis</subject><subject>Streptococcal Infections - blood</subject><subject>Streptococcal Infections - diagnosis</subject><subject>Streptococcal Infections - microbiology</subject><subject>Streptococcus</subject><subject>Streptococcus agalactiae</subject><subject>Streptococcus agalactiae - genetics</subject><subject>Streptococcus agalactiae - isolation & purification</subject><subject>Streptococcus infections</subject><subject>temperature</subject><subject>Temperature requirements</subject><subject>therapeutics</subject><issn>0301-4851</issn><issn>1573-4978</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGO1SAUhonRONfRF3BhSNyMC_RQCpTlzERHk2s0UdeEAlUmbalAF_c1fGK506smLnTFgu_8P4cPoacUXlIA-SpTCowRaFoCSgIncA_tKJeMtEp299EOGFDSdpyeoUc53wJASyV_iM6YAmiAdTv0Yx_jQibvgine4ZBj-ebTZEZspmUMQ7CmhDjji_3l-48vsMnZHPAQE_YmjQccZ5JD8dj54u0dGAd8k-K64Cv8qSS_lGijtWvGYR5OSJjx7ONsSm3Jfskh436M0eF87PSP0YPBjNk_OZ3n6Mub15-v35L9h5t315d7YptOFTJYwyilggvGmXMKZM-UcEoZZZ1zrP6NV0J0omtcL2zTKOB8MEpa6_rWMnaOLrbcJcXvq89FTyFbP46mvm7NmlHOJJWStf9HQSrFuRDH1Od_obdxTXNdZKNYKzpVqWajbIo5Jz_oJYXJpIOmoI9y9SZXV7n6Tq6GOvTsFL321djvkV82K8A2INer-atPf7r_EfsTICav_Q</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Banerjee, Shukla</creator><creator>K., Mithun H.</creator><creator>Shastry, Rajesh P.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-8627-9759</orcidid></search><sort><creationdate>20241201</creationdate><title>Loop-mediated isothermal amplification (LAMP) assay for early on-site detection of Group B Streptococcus infection in neonatal sepsis blood sample</title><author>Banerjee, Shukla ; K., Mithun H. ; Shastry, Rajesh P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c289t-fca3111656353dd907b396d99a9cddd3103e9668682db6c229055fa97ccdb4c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>blood</topic><topic>blood sampling</topic><topic>Colorimetry</topic><topic>DNA</topic><topic>DNA, Bacterial - blood</topic><topic>DNA, Bacterial - genetics</topic><topic>Gene amplification</topic><topic>genes</topic><topic>Histology</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Life Sciences</topic><topic>loop-mediated isothermal amplification</topic><topic>Medical innovations</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Morphology</topic><topic>Neonatal Sepsis - blood</topic><topic>Neonatal Sepsis - diagnosis</topic><topic>Neonatal Sepsis - microbiology</topic><topic>Neonates</topic><topic>Nucleic Acid Amplification Techniques - methods</topic><topic>Onsite</topic><topic>Original Article</topic><topic>risk</topic><topic>Sensitivity and Specificity</topic><topic>Sepsis</topic><topic>Streptococcal Infections - blood</topic><topic>Streptococcal Infections - diagnosis</topic><topic>Streptococcal Infections - microbiology</topic><topic>Streptococcus</topic><topic>Streptococcus agalactiae</topic><topic>Streptococcus agalactiae - genetics</topic><topic>Streptococcus agalactiae - isolation & purification</topic><topic>Streptococcus infections</topic><topic>temperature</topic><topic>Temperature requirements</topic><topic>therapeutics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Banerjee, Shukla</creatorcontrib><creatorcontrib>K., Mithun H.</creatorcontrib><creatorcontrib>Shastry, Rajesh P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Banerjee, Shukla</au><au>K., Mithun H.</au><au>Shastry, Rajesh P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Loop-mediated isothermal amplification (LAMP) assay for early on-site detection of Group B Streptococcus infection in neonatal sepsis blood sample</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>51</volume><issue>1</issue><spage>811</spage><epage>811</epage><pages>811-811</pages><artnum>811</artnum><issn>0301-4851</issn><issn>1573-4978</issn><eissn>1573-4978</eissn><abstract>Background
Neonatal sepsis, often attributed to Group B
Streptococcus
(GBS) infection, poses a critical health risk to infants, demanding rapid and accurate diagnostic approaches. Existing diagnostic approaches are dependent on traditional culture methods, a process that requires substantial time and has the potential to delay crucial therapeutic assessments.
Methods
This study introduces an innovative Loop-Mediated Isothermal Amplification (LAMP) assay for the early on-site detection of GBS infection from neonatal sepsis blood samples. To develop a LAMP assay, the primers are designed for the selective targeting of a highly conserved segment within the
cfb
gene encoding the CAMP factor in
Streptococcus agalactiae
ensuring high specificity.
Results
Rigorous optimization of reaction conditions, including temperature and incubation time, enhances the efficiency of the LAMP assay, enabling rapid and reliable GBS detection within a short timeframe. The diagnostic efficacy of the LAMP assay was evaluated using spiked blood samples by eliminating the DNA extraction step. The simplified colorimetric LAMP assay has the capability to detect
S. agalactiae
in a neonatal blood sample containing 2 CFU/mL during sepsis. Additionally, the LAMP assay effectively detected
S. agalactiae
in both the standard and spiked blood samples, with no detectable interference with blood.
Conclusion
This optimised LAMP assay emerges as a promising tool for early GBS detection, offering a rapid and accurate on-site solution that has the potential to inform timely interventions and improve outcomes in neonatal sepsis cases.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>39002038</pmid><doi>10.1007/s11033-024-09705-0</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-8627-9759</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4851 |
| ispartof | Molecular biology reports, 2024-12, Vol.51 (1), p.811-811, Article 811 |
| issn | 0301-4851 1573-4978 1573-4978 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3153717734 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animal Anatomy Animal Biochemistry Bacterial Proteins - genetics Biomedical and Life Sciences blood blood sampling Colorimetry DNA DNA, Bacterial - blood DNA, Bacterial - genetics Gene amplification genes Histology Humans Infant, Newborn Life Sciences loop-mediated isothermal amplification Medical innovations Molecular Diagnostic Techniques - methods Morphology Neonatal Sepsis - blood Neonatal Sepsis - diagnosis Neonatal Sepsis - microbiology Neonates Nucleic Acid Amplification Techniques - methods Onsite Original Article risk Sensitivity and Specificity Sepsis Streptococcal Infections - blood Streptococcal Infections - diagnosis Streptococcal Infections - microbiology Streptococcus Streptococcus agalactiae Streptococcus agalactiae - genetics Streptococcus agalactiae - isolation & purification Streptococcus infections temperature Temperature requirements therapeutics |
| title | Loop-mediated isothermal amplification (LAMP) assay for early on-site detection of Group B Streptococcus infection in neonatal sepsis blood sample |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T07%3A12%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Loop-mediated%20isothermal%20amplification%20(LAMP)%20assay%20for%20early%20on-site%20detection%20of%20Group%20B%20Streptococcus%20infection%20in%20neonatal%20sepsis%20blood%20sample&rft.jtitle=Molecular%20biology%20reports&rft.au=Banerjee,%20Shukla&rft.date=2024-12-01&rft.volume=51&rft.issue=1&rft.spage=811&rft.epage=811&rft.pages=811-811&rft.artnum=811&rft.issn=0301-4851&rft.eissn=1573-4978&rft_id=info:doi/10.1007/s11033-024-09705-0&rft_dat=%3Cproquest_cross%3E3079955663%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3079934689&rft_id=info:pmid/39002038&rfr_iscdi=true |