Closed-tube saltatory rolling circle amplification assay for rapid and visual detection of allergenic cashew nut in foods
Cashew nut allergy shows an increasing prevalence along with its high consumption. Nowadays, food allergy still has no cure, and allergen avoidance is still the standard management, urging a sensitive and convenient cashew nut detection method. Herein, saltatory rolling circle amplification (SRCA),...
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| Veröffentlicht in: | Food control 2024-12, Vol.166, p.110769, Article 110769 |
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| creator | Chen, Yingying Guo, Zipeng Sun, Zhaoqing Wu, Xinyu Xu, Yifan Jin, Zhengye Cheng, Yuying She, Zhen Jiang, Qianqian Wo, Yuxin Wu, Ting Liu, Hongwang Liu, Meihui Liu, Yulu Diao, Enjie Li, Dengchao Mao, Ruifeng |
| description | Cashew nut allergy shows an increasing prevalence along with its high consumption. Nowadays, food allergy still has no cure, and allergen avoidance is still the standard management, urging a sensitive and convenient cashew nut detection method. Herein, saltatory rolling circle amplification (SRCA), loop-mediated isothermal amplification (LAMP), as well as polymerase chain reaction (PCR) targeting the Ana o 2 gene were proposed and compared. In addition, a closed-tube SRCA assay using hydroxynaphthol blue (HNB) as a color indicator (HNB-SRCA) was developed to realize visual on-site detection of cashew nut. SRCA and HNB-SRCA can detect 75.3 fg of cashew nut DNA, which is 10- and 100-times more sensitive than LAMP and PCR, respectively. SRCA and HNB-SRCA exhibited a relative sensitivity of 0.01% in a binary mixture, which is comparable to LAMP and 10-times higher than that of PCR. When combined with a rapid DNA extraction method, SRCA and HNB-SRCA performed better applicability compared to LAMP and PCR. Additionally, compared to SRCA using electrophoresis to analyze the amplification products, the visual closed-tube HNB-SRCA combined with the rapid DNA extraction protocol is a better potential cashew nut on-site detection strategy in foods.
•A rapid visual HNB-SRCA for on-site detection of cashew nut has been developed.•HNB-SRCA assay exhibits high sensitivity, detection 75.3 fg of target DNA.•The present assay exhibits high applicability for the rapid DNA extraction process. |
| doi_str_mv | 10.1016/j.foodcont.2024.110769 |
| format | Article |
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•A rapid visual HNB-SRCA for on-site detection of cashew nut has been developed.•HNB-SRCA assay exhibits high sensitivity, detection 75.3 fg of target DNA.•The present assay exhibits high applicability for the rapid DNA extraction process.</description><identifier>ISSN: 0956-7135</identifier><identifier>DOI: 10.1016/j.foodcont.2024.110769</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Allergen detection ; allergenicity ; allergens ; Anacardium occidentale L ; cashew nuts ; color ; DNA ; electrophoresis ; food allergies ; Food allergy ; food safety ; genes ; Isothermal assay ; loop-mediated isothermal amplification ; polymerase chain reaction ; protocols</subject><ispartof>Food control, 2024-12, Vol.166, p.110769, Article 110769</ispartof><rights>2024 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c222t-42d43c17b75290fd161b11967f1d4202cb58155787367f9ac04be4d27cfa9ef73</cites><orcidid>0000-0001-8204-571X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956713524004869$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Chen, Yingying</creatorcontrib><creatorcontrib>Guo, Zipeng</creatorcontrib><creatorcontrib>Sun, Zhaoqing</creatorcontrib><creatorcontrib>Wu, Xinyu</creatorcontrib><creatorcontrib>Xu, Yifan</creatorcontrib><creatorcontrib>Jin, Zhengye</creatorcontrib><creatorcontrib>Cheng, Yuying</creatorcontrib><creatorcontrib>She, Zhen</creatorcontrib><creatorcontrib>Jiang, Qianqian</creatorcontrib><creatorcontrib>Wo, Yuxin</creatorcontrib><creatorcontrib>Wu, Ting</creatorcontrib><creatorcontrib>Liu, Hongwang</creatorcontrib><creatorcontrib>Liu, Meihui</creatorcontrib><creatorcontrib>Liu, Yulu</creatorcontrib><creatorcontrib>Diao, Enjie</creatorcontrib><creatorcontrib>Li, Dengchao</creatorcontrib><creatorcontrib>Mao, Ruifeng</creatorcontrib><title>Closed-tube saltatory rolling circle amplification assay for rapid and visual detection of allergenic cashew nut in foods</title><title>Food control</title><description>Cashew nut allergy shows an increasing prevalence along with its high consumption. Nowadays, food allergy still has no cure, and allergen avoidance is still the standard management, urging a sensitive and convenient cashew nut detection method. Herein, saltatory rolling circle amplification (SRCA), loop-mediated isothermal amplification (LAMP), as well as polymerase chain reaction (PCR) targeting the Ana o 2 gene were proposed and compared. In addition, a closed-tube SRCA assay using hydroxynaphthol blue (HNB) as a color indicator (HNB-SRCA) was developed to realize visual on-site detection of cashew nut. SRCA and HNB-SRCA can detect 75.3 fg of cashew nut DNA, which is 10- and 100-times more sensitive than LAMP and PCR, respectively. SRCA and HNB-SRCA exhibited a relative sensitivity of 0.01% in a binary mixture, which is comparable to LAMP and 10-times higher than that of PCR. When combined with a rapid DNA extraction method, SRCA and HNB-SRCA performed better applicability compared to LAMP and PCR. Additionally, compared to SRCA using electrophoresis to analyze the amplification products, the visual closed-tube HNB-SRCA combined with the rapid DNA extraction protocol is a better potential cashew nut on-site detection strategy in foods.
•A rapid visual HNB-SRCA for on-site detection of cashew nut has been developed.•HNB-SRCA assay exhibits high sensitivity, detection 75.3 fg of target DNA.•The present assay exhibits high applicability for the rapid DNA extraction process.</description><subject>Allergen detection</subject><subject>allergenicity</subject><subject>allergens</subject><subject>Anacardium occidentale L</subject><subject>cashew nuts</subject><subject>color</subject><subject>DNA</subject><subject>electrophoresis</subject><subject>food allergies</subject><subject>Food allergy</subject><subject>food safety</subject><subject>genes</subject><subject>Isothermal assay</subject><subject>loop-mediated isothermal amplification</subject><subject>polymerase chain reaction</subject><subject>protocols</subject><issn>0956-7135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkDtPAzEQhK8AifD4C8glzQWv7-FcB4p4SZFooLZ89jo4cuxg-4Lu33MhUFOtNJoZ7XxFcQ10DhTa283chKBV8HnOKKvnAJS33Ukxo13Tlhyq5qw4T2lDKXAKdFaMSxcS6jIPPZIkXZY5xJHE4Jz1a6JsVA6J3O6cNVbJbIMnMiU5EhMiiXJnNZFek71Ng3REY0b1YwqGSOcwrtFbRZRMH_hF_JCJ9eTwY7osTo10Ca9-70Xx_vjwtnwuV69PL8v7VakYY7msma4rBbznDeuo0dBCD9C13ICup42qbxbQNHzBq0nrpKJ1j7VmXBnZoeHVRXFz7N3F8DlgymJrk0LnpMcwJFFBU3FoKWOTtT1aVQwpRTRiF-1WxlEAFQe-YiP--IoDX3HkOwXvjkGchuwtRpGURa9Q2zjxEDrY_yq-AYaki1o</recordid><startdate>202412</startdate><enddate>202412</enddate><creator>Chen, Yingying</creator><creator>Guo, Zipeng</creator><creator>Sun, Zhaoqing</creator><creator>Wu, Xinyu</creator><creator>Xu, Yifan</creator><creator>Jin, Zhengye</creator><creator>Cheng, Yuying</creator><creator>She, Zhen</creator><creator>Jiang, Qianqian</creator><creator>Wo, Yuxin</creator><creator>Wu, Ting</creator><creator>Liu, Hongwang</creator><creator>Liu, Meihui</creator><creator>Liu, Yulu</creator><creator>Diao, Enjie</creator><creator>Li, Dengchao</creator><creator>Mao, Ruifeng</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-8204-571X</orcidid></search><sort><creationdate>202412</creationdate><title>Closed-tube saltatory rolling circle amplification assay for rapid and visual detection of allergenic cashew nut in foods</title><author>Chen, Yingying ; Guo, Zipeng ; Sun, Zhaoqing ; Wu, Xinyu ; Xu, Yifan ; Jin, Zhengye ; Cheng, Yuying ; She, Zhen ; Jiang, Qianqian ; Wo, Yuxin ; Wu, Ting ; Liu, Hongwang ; Liu, Meihui ; Liu, Yulu ; Diao, Enjie ; Li, Dengchao ; Mao, Ruifeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c222t-42d43c17b75290fd161b11967f1d4202cb58155787367f9ac04be4d27cfa9ef73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Allergen detection</topic><topic>allergenicity</topic><topic>allergens</topic><topic>Anacardium occidentale L</topic><topic>cashew nuts</topic><topic>color</topic><topic>DNA</topic><topic>electrophoresis</topic><topic>food allergies</topic><topic>Food allergy</topic><topic>food safety</topic><topic>genes</topic><topic>Isothermal assay</topic><topic>loop-mediated isothermal amplification</topic><topic>polymerase chain reaction</topic><topic>protocols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Yingying</creatorcontrib><creatorcontrib>Guo, Zipeng</creatorcontrib><creatorcontrib>Sun, Zhaoqing</creatorcontrib><creatorcontrib>Wu, Xinyu</creatorcontrib><creatorcontrib>Xu, Yifan</creatorcontrib><creatorcontrib>Jin, Zhengye</creatorcontrib><creatorcontrib>Cheng, Yuying</creatorcontrib><creatorcontrib>She, Zhen</creatorcontrib><creatorcontrib>Jiang, Qianqian</creatorcontrib><creatorcontrib>Wo, Yuxin</creatorcontrib><creatorcontrib>Wu, Ting</creatorcontrib><creatorcontrib>Liu, Hongwang</creatorcontrib><creatorcontrib>Liu, Meihui</creatorcontrib><creatorcontrib>Liu, Yulu</creatorcontrib><creatorcontrib>Diao, Enjie</creatorcontrib><creatorcontrib>Li, Dengchao</creatorcontrib><creatorcontrib>Mao, Ruifeng</creatorcontrib><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Food control</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Yingying</au><au>Guo, Zipeng</au><au>Sun, Zhaoqing</au><au>Wu, Xinyu</au><au>Xu, Yifan</au><au>Jin, Zhengye</au><au>Cheng, Yuying</au><au>She, Zhen</au><au>Jiang, Qianqian</au><au>Wo, Yuxin</au><au>Wu, Ting</au><au>Liu, Hongwang</au><au>Liu, Meihui</au><au>Liu, Yulu</au><au>Diao, Enjie</au><au>Li, Dengchao</au><au>Mao, Ruifeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Closed-tube saltatory rolling circle amplification assay for rapid and visual detection of allergenic cashew nut in foods</atitle><jtitle>Food control</jtitle><date>2024-12</date><risdate>2024</risdate><volume>166</volume><spage>110769</spage><pages>110769-</pages><artnum>110769</artnum><issn>0956-7135</issn><abstract>Cashew nut allergy shows an increasing prevalence along with its high consumption. Nowadays, food allergy still has no cure, and allergen avoidance is still the standard management, urging a sensitive and convenient cashew nut detection method. Herein, saltatory rolling circle amplification (SRCA), loop-mediated isothermal amplification (LAMP), as well as polymerase chain reaction (PCR) targeting the Ana o 2 gene were proposed and compared. In addition, a closed-tube SRCA assay using hydroxynaphthol blue (HNB) as a color indicator (HNB-SRCA) was developed to realize visual on-site detection of cashew nut. SRCA and HNB-SRCA can detect 75.3 fg of cashew nut DNA, which is 10- and 100-times more sensitive than LAMP and PCR, respectively. SRCA and HNB-SRCA exhibited a relative sensitivity of 0.01% in a binary mixture, which is comparable to LAMP and 10-times higher than that of PCR. When combined with a rapid DNA extraction method, SRCA and HNB-SRCA performed better applicability compared to LAMP and PCR. Additionally, compared to SRCA using electrophoresis to analyze the amplification products, the visual closed-tube HNB-SRCA combined with the rapid DNA extraction protocol is a better potential cashew nut on-site detection strategy in foods.
•A rapid visual HNB-SRCA for on-site detection of cashew nut has been developed.•HNB-SRCA assay exhibits high sensitivity, detection 75.3 fg of target DNA.•The present assay exhibits high applicability for the rapid DNA extraction process.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.foodcont.2024.110769</doi><orcidid>https://orcid.org/0000-0001-8204-571X</orcidid></addata></record> |
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| subjects | Allergen detection allergenicity allergens Anacardium occidentale L cashew nuts color DNA electrophoresis food allergies Food allergy food safety genes Isothermal assay loop-mediated isothermal amplification polymerase chain reaction protocols |
| title | Closed-tube saltatory rolling circle amplification assay for rapid and visual detection of allergenic cashew nut in foods |
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