Profile of miRNA expression in the hippocampus of epileptic mice and the prediction of potential therapeutic targets
Epilepsy is a common neurological disease. Increasing evidence has highlighted the role of miRNAs in the molecular mechanisms underlying the development of neurological diseases such as epilepsy. In this study, we established a lithium chloride–pilocarpine epilepsy mouse model, performed miRNA seque...
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| description | Epilepsy is a common neurological disease. Increasing evidence has highlighted the role of miRNAs in the molecular mechanisms underlying the development of neurological diseases such as epilepsy. In this study, we established a lithium chloride–pilocarpine epilepsy mouse model, performed miRNA sequencing of hippocampal tissue samples, and compared the obtained miRNA expression profile with that of normal control mice to determine differences in expression levels. We found that 55 miRNAs were differentially expressed in status epilepticus mice compared with normal control mice, with 38 upregulated and 17 downregulated miRNAs. Through subsequent analysis of the five downregulated miRNAs (mmu-let-7a-1-3p, mmu-let-7a-2-3p, mmu-let-7c-5p, mmu-let-7d-5p, and mmu-let-7e-5p) with the most significant differences in expression, the key pathways involved included the MAPK signaling pathway and focal adhesion, among others. Therefore, we believe that let-7 family miRNAs may be potential therapeutic targets for epilepsy. |
| doi_str_mv | 10.1007/s11033-024-09861-3 |
| format | Article |
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Increasing evidence has highlighted the role of miRNAs in the molecular mechanisms underlying the development of neurological diseases such as epilepsy. In this study, we established a lithium chloride–pilocarpine epilepsy mouse model, performed miRNA sequencing of hippocampal tissue samples, and compared the obtained miRNA expression profile with that of normal control mice to determine differences in expression levels. We found that 55 miRNAs were differentially expressed in status epilepticus mice compared with normal control mice, with 38 upregulated and 17 downregulated miRNAs. Through subsequent analysis of the five downregulated miRNAs (mmu-let-7a-1-3p, mmu-let-7a-2-3p, mmu-let-7c-5p, mmu-let-7d-5p, and mmu-let-7e-5p) with the most significant differences in expression, the key pathways involved included the MAPK signaling pathway and focal adhesion, among others. Therefore, we believe that let-7 family miRNAs may be potential therapeutic targets for epilepsy.</description><identifier>ISSN: 0301-4851</identifier><identifier>ISSN: 1573-4978</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-024-09861-3</identifier><identifier>PMID: 39172288</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Animals ; Biomedical and Life Sciences ; Brain research ; Convulsions & seizures ; Disease Models, Animal ; Down-regulation ; Epilepsy ; Epilepsy - genetics ; Epilepsy - metabolism ; family ; focal adhesions ; Gene Expression Profiling - methods ; Gene Expression Regulation ; Hippocampus ; Hippocampus - metabolism ; Histology ; Hospitals ; Life Sciences ; Lithium ; Lithium chloride ; Lithium Chloride - pharmacology ; Male ; MAP kinase ; Medical research ; Mice ; microRNA ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; Molecular biology ; Molecular modelling ; Morphology ; Neurological diseases ; Original Article ; Physiology ; Pilocarpine ; prediction ; Reagents ; Ribonucleic acid ; RNA ; Signal transduction ; Status Epilepticus - chemically induced ; Status Epilepticus - genetics ; Status Epilepticus - metabolism ; Therapeutic targets ; therapeutics</subject><ispartof>Molecular biology reports, 2024-12, Vol.51 (1), p.929-929, Article 929</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024 Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><rights>Copyright Springer Nature B.V. 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Increasing evidence has highlighted the role of miRNAs in the molecular mechanisms underlying the development of neurological diseases such as epilepsy. In this study, we established a lithium chloride–pilocarpine epilepsy mouse model, performed miRNA sequencing of hippocampal tissue samples, and compared the obtained miRNA expression profile with that of normal control mice to determine differences in expression levels. We found that 55 miRNAs were differentially expressed in status epilepticus mice compared with normal control mice, with 38 upregulated and 17 downregulated miRNAs. Through subsequent analysis of the five downregulated miRNAs (mmu-let-7a-1-3p, mmu-let-7a-2-3p, mmu-let-7c-5p, mmu-let-7d-5p, and mmu-let-7e-5p) with the most significant differences in expression, the key pathways involved included the MAPK signaling pathway and focal adhesion, among others. Therefore, we believe that let-7 family miRNAs may be potential therapeutic targets for epilepsy.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Brain research</subject><subject>Convulsions & seizures</subject><subject>Disease Models, Animal</subject><subject>Down-regulation</subject><subject>Epilepsy</subject><subject>Epilepsy - genetics</subject><subject>Epilepsy - metabolism</subject><subject>family</subject><subject>focal adhesions</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation</subject><subject>Hippocampus</subject><subject>Hippocampus - metabolism</subject><subject>Histology</subject><subject>Hospitals</subject><subject>Life Sciences</subject><subject>Lithium</subject><subject>Lithium chloride</subject><subject>Lithium Chloride - pharmacology</subject><subject>Male</subject><subject>MAP kinase</subject><subject>Medical research</subject><subject>Mice</subject><subject>microRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>Molecular biology</subject><subject>Molecular modelling</subject><subject>Morphology</subject><subject>Neurological diseases</subject><subject>Original Article</subject><subject>Physiology</subject><subject>Pilocarpine</subject><subject>prediction</subject><subject>Reagents</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Signal transduction</subject><subject>Status Epilepticus - chemically induced</subject><subject>Status Epilepticus - genetics</subject><subject>Status Epilepticus - metabolism</subject><subject>Therapeutic targets</subject><subject>therapeutics</subject><issn>0301-4851</issn><issn>1573-4978</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U2L1TAUBuAginMd_QMupODGTfWcpGna5TD4BYOK6Dq06clMhraJSQr6703vHRVc6CqL87wnJC9jTxFeIoB6lRBBiBp4U0PftViLe-yAUom66VV3nx1AANZNJ_GMPUrpFgAaVPIhOxM9Ks677sDyp-itm6nytlrc5w8XFX0PkVJyfq3cWuUbqm5cCN4MS9jSzigUH7IzJWCoGtbpqEpqcibvuYKCz7RmN8z7LA6Btj2Qh3hNOT1mD-wwJ3pyd56zr29ef7l8V199fPv-8uKqNkL2uRatbRqwnFs5gVGtJIG85XJqp1b2RnFjQZEqfzHy0UxcEhc4WoTRcJAcxDl7cdobov-2Ucp6ccnQPA8r-S1pgVIolKDU_yn0su1A9G2hz_-it36La3nIUXXYc9UVxU_KRJ9SJKtDdMsQf2gEvdenT_XpUp8-1qdFCT27W72NC02_I7_6KkCcQCqj9Zrin7v_sfYnCiSk7g</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Tan, Yafu</creator><creator>Yu, Yongjia</creator><creator>Niu, Haodong</creator><creator>Wang, Chunxi</creator><creator>Mo, Panlin</creator><creator>Li, Dongqiao</creator><creator>Zhang, Qing</creator><creator>Feng, Daqin</creator><creator>Liu, Chang</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20241201</creationdate><title>Profile of miRNA expression in the hippocampus of epileptic mice and the prediction of potential therapeutic targets</title><author>Tan, Yafu ; 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Increasing evidence has highlighted the role of miRNAs in the molecular mechanisms underlying the development of neurological diseases such as epilepsy. In this study, we established a lithium chloride–pilocarpine epilepsy mouse model, performed miRNA sequencing of hippocampal tissue samples, and compared the obtained miRNA expression profile with that of normal control mice to determine differences in expression levels. We found that 55 miRNAs were differentially expressed in status epilepticus mice compared with normal control mice, with 38 upregulated and 17 downregulated miRNAs. Through subsequent analysis of the five downregulated miRNAs (mmu-let-7a-1-3p, mmu-let-7a-2-3p, mmu-let-7c-5p, mmu-let-7d-5p, and mmu-let-7e-5p) with the most significant differences in expression, the key pathways involved included the MAPK signaling pathway and focal adhesion, among others. Therefore, we believe that let-7 family miRNAs may be potential therapeutic targets for epilepsy.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>39172288</pmid><doi>10.1007/s11033-024-09861-3</doi><tpages>1</tpages></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Animals Biomedical and Life Sciences Brain research Convulsions & seizures Disease Models, Animal Down-regulation Epilepsy Epilepsy - genetics Epilepsy - metabolism family focal adhesions Gene Expression Profiling - methods Gene Expression Regulation Hippocampus Hippocampus - metabolism Histology Hospitals Life Sciences Lithium Lithium chloride Lithium Chloride - pharmacology Male MAP kinase Medical research Mice microRNA MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA Molecular biology Molecular modelling Morphology Neurological diseases Original Article Physiology Pilocarpine prediction Reagents Ribonucleic acid RNA Signal transduction Status Epilepticus - chemically induced Status Epilepticus - genetics Status Epilepticus - metabolism Therapeutic targets therapeutics |
| title | Profile of miRNA expression in the hippocampus of epileptic mice and the prediction of potential therapeutic targets |
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