A rationally optimised batch bioreactor cultivation of Viola odorata plant cells for sustainable production of its key bioactive principles
Viola odorata plant extracts are extensively used in indigenous medicine to treat respiratory diseases. V. odorata’s limited availability in nature, due to geographical conditions and adulteration, makes continuous supply of high-quality plant raw material challenging. This results in low-quality pl...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2024-08, Vol.158 (2), p.35-35, Article 35 |
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| creator | Babu, R. Srivastava, Smita |
| description | Viola odorata
plant extracts are extensively used in indigenous medicine to treat respiratory diseases.
V. odorata’s
limited availability in nature, due to geographical conditions and adulteration, makes continuous supply of high-quality plant raw material challenging. This results in low-quality plant extracts that can have inconsistent and sometimes adverse effects when used in medicine. As an effective solution, in vitro plant biomass cultivation conserves plant diversity, ensuring high-quality raw materials with uniform quality and quantity. Toward this, the current study focuses on establishing bioprocess strategy to achieve maximum.
V. odorata
biomass productivity up to bioreactor level. In this context, in vitro culture conditions were rationally optimised, focusing upon both statistical optimization and one-factor-at-a-time (OFAT) approach, for improved biomass productivity. Inoculum density, macronutrient concentration, shaking speed, temperature, pH and light intensity were systematically optimized to achieve the maximum biomass. This led to increased biomass production from 10.2 ± 0.8 g DW L
− 1
to 21.7 ± 0.8 g DW L
− 1
within 12 days of the batch cultivation period. Optimized culture conditions were implemented in three different types of bioreactors and cultivation in a stirred tank reactor produced ∼ 19.7 g DW L
− 1
of maximum biomass within a 12 day batch cultivation period. Further, the metabolite profile of the bioreactor cultivated biomass (alcoholic extract) was compared with that of the natural plant biomass using gas chromatography-mass spectrometry-based metabolomics. The antibacterial activity of the plant extract was tested against three bacteria responsible for respiratory tract infections, and their antioxidant activity was compared with that of Vitamin E, wherein the results demonstrated comparable activity. The novelty of this study is in the fact that the
V. odorata
plant cells were cultivated in bioreactors for the first time for potential medicinal applications. It provides an alternative method for sustainable production of plant biomass, thereby conserving the natural plant sources.
Graphical Abstract
Key message
Bioreactor cultivation of
V. odorata
plant cells with biomass productivity equivalent to that in shake flask culture, yielding extracts with a similar metabolite profile and associated bioactivity to that in the natural plant extracts. |
| doi_str_mv | 10.1007/s11240-024-02829-y |
| format | Article |
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plant extracts are extensively used in indigenous medicine to treat respiratory diseases.
V. odorata’s
limited availability in nature, due to geographical conditions and adulteration, makes continuous supply of high-quality plant raw material challenging. This results in low-quality plant extracts that can have inconsistent and sometimes adverse effects when used in medicine. As an effective solution, in vitro plant biomass cultivation conserves plant diversity, ensuring high-quality raw materials with uniform quality and quantity. Toward this, the current study focuses on establishing bioprocess strategy to achieve maximum.
V. odorata
biomass productivity up to bioreactor level. In this context, in vitro culture conditions were rationally optimised, focusing upon both statistical optimization and one-factor-at-a-time (OFAT) approach, for improved biomass productivity. Inoculum density, macronutrient concentration, shaking speed, temperature, pH and light intensity were systematically optimized to achieve the maximum biomass. This led to increased biomass production from 10.2 ± 0.8 g DW L
− 1
to 21.7 ± 0.8 g DW L
− 1
within 12 days of the batch cultivation period. Optimized culture conditions were implemented in three different types of bioreactors and cultivation in a stirred tank reactor produced ∼ 19.7 g DW L
− 1
of maximum biomass within a 12 day batch cultivation period. Further, the metabolite profile of the bioreactor cultivated biomass (alcoholic extract) was compared with that of the natural plant biomass using gas chromatography-mass spectrometry-based metabolomics. The antibacterial activity of the plant extract was tested against three bacteria responsible for respiratory tract infections, and their antioxidant activity was compared with that of Vitamin E, wherein the results demonstrated comparable activity. The novelty of this study is in the fact that the
V. odorata
plant cells were cultivated in bioreactors for the first time for potential medicinal applications. It provides an alternative method for sustainable production of plant biomass, thereby conserving the natural plant sources.
Graphical Abstract
Key message
Bioreactor cultivation of
V. odorata
plant cells with biomass productivity equivalent to that in shake flask culture, yielding extracts with a similar metabolite profile and associated bioactivity to that in the natural plant extracts.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-024-02829-y</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>adulterated products ; Antibacterial activity ; antibacterial properties ; antioxidant activity ; Biological activity ; Biomass ; biomass production ; Biomedical and Life Sciences ; bioprocessing ; Bioreactors ; Cell culture ; Cultivation ; Culture ; Gas chromatography ; Inoculum ; inoculum density ; Life Sciences ; Light intensity ; Luminous intensity ; Mass spectrometry ; Mass spectroscopy ; Medicinal plants ; Metabolites ; Metabolomics ; Original Article ; phytomass ; Plant biomass ; Plant cells ; Plant diversity ; Plant extracts ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Productivity ; Raw materials ; Respiratory diseases ; respiratory system ; Respiratory tract ; Respiratory tract infection ; Shaking ; species diversity ; Sustainable development ; Sustainable production ; temperature ; traditional medicine ; Viola odorata ; Vitamin E</subject><ispartof>Plant cell, tissue and organ culture, 2024-08, Vol.158 (2), p.35-35, Article 35</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c233t-2ff987904dbaa61c21e4cc7946f6a39348ff89cb0a8359c1c776b9a408d935133</cites><orcidid>0000-0001-9582-3904</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-024-02829-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-024-02829-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Babu, R.</creatorcontrib><creatorcontrib>Srivastava, Smita</creatorcontrib><title>A rationally optimised batch bioreactor cultivation of Viola odorata plant cells for sustainable production of its key bioactive principles</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>Viola odorata
plant extracts are extensively used in indigenous medicine to treat respiratory diseases.
V. odorata’s
limited availability in nature, due to geographical conditions and adulteration, makes continuous supply of high-quality plant raw material challenging. This results in low-quality plant extracts that can have inconsistent and sometimes adverse effects when used in medicine. As an effective solution, in vitro plant biomass cultivation conserves plant diversity, ensuring high-quality raw materials with uniform quality and quantity. Toward this, the current study focuses on establishing bioprocess strategy to achieve maximum.
V. odorata
biomass productivity up to bioreactor level. In this context, in vitro culture conditions were rationally optimised, focusing upon both statistical optimization and one-factor-at-a-time (OFAT) approach, for improved biomass productivity. Inoculum density, macronutrient concentration, shaking speed, temperature, pH and light intensity were systematically optimized to achieve the maximum biomass. This led to increased biomass production from 10.2 ± 0.8 g DW L
− 1
to 21.7 ± 0.8 g DW L
− 1
within 12 days of the batch cultivation period. Optimized culture conditions were implemented in three different types of bioreactors and cultivation in a stirred tank reactor produced ∼ 19.7 g DW L
− 1
of maximum biomass within a 12 day batch cultivation period. Further, the metabolite profile of the bioreactor cultivated biomass (alcoholic extract) was compared with that of the natural plant biomass using gas chromatography-mass spectrometry-based metabolomics. The antibacterial activity of the plant extract was tested against three bacteria responsible for respiratory tract infections, and their antioxidant activity was compared with that of Vitamin E, wherein the results demonstrated comparable activity. The novelty of this study is in the fact that the
V. odorata
plant cells were cultivated in bioreactors for the first time for potential medicinal applications. It provides an alternative method for sustainable production of plant biomass, thereby conserving the natural plant sources.
Graphical Abstract
Key message
Bioreactor cultivation of
V. odorata
plant cells with biomass productivity equivalent to that in shake flask culture, yielding extracts with a similar metabolite profile and associated bioactivity to that in the natural plant extracts.</description><subject>adulterated products</subject><subject>Antibacterial activity</subject><subject>antibacterial properties</subject><subject>antioxidant activity</subject><subject>Biological activity</subject><subject>Biomass</subject><subject>biomass production</subject><subject>Biomedical and Life Sciences</subject><subject>bioprocessing</subject><subject>Bioreactors</subject><subject>Cell culture</subject><subject>Cultivation</subject><subject>Culture</subject><subject>Gas chromatography</subject><subject>Inoculum</subject><subject>inoculum density</subject><subject>Life Sciences</subject><subject>Light intensity</subject><subject>Luminous intensity</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medicinal plants</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Original Article</subject><subject>phytomass</subject><subject>Plant biomass</subject><subject>Plant cells</subject><subject>Plant diversity</subject><subject>Plant extracts</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Productivity</subject><subject>Raw materials</subject><subject>Respiratory diseases</subject><subject>respiratory system</subject><subject>Respiratory tract</subject><subject>Respiratory tract infection</subject><subject>Shaking</subject><subject>species diversity</subject><subject>Sustainable development</subject><subject>Sustainable production</subject><subject>temperature</subject><subject>traditional medicine</subject><subject>Viola odorata</subject><subject>Vitamin E</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kc1u3SAQhVGVSL35eYGukLrJxu1gsDHLKOqfFKmbNFs0xpCSEnMDOJKfoS8dnNuqUhdZoFnwnaOZcwh5x-ADA5AfM2OtgAZaUd_QqmZ9Q3ask7zpQIgjsgPWy6YfOvmWnOR8DwA9F2xHfl_ShMXHGUNYadwX_-CzneiIxfyko4_JoikxUbOE4p9eUBodvfUxII1TrGqk-4BzocaGkKmrcF5yQT_jGCzdpzgt5q_Ol0x_2XVzrr7-afv3s_H7YPMZOXYYsj3_M0_Jj8-fbq6-Ntffv3y7urxuTMt5aVrn1CAViGlE7JlpmRXGSCV61yNXXAzODcqMgAPvlGFGyn5UKGCYFO8Y56fk4uBbV3tcbC663rwtj7ONS9acdVyCklxU9P1_6H1cUg2rUqAA1FAzrVR7oEyKOSfrdD3qAdOqGeitH33oR9d-9Es_eq0ifhDlLYE7m_5Zv6J6BhT7lj8</recordid><startdate>20240801</startdate><enddate>20240801</enddate><creator>Babu, R.</creator><creator>Srivastava, Smita</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-9582-3904</orcidid></search><sort><creationdate>20240801</creationdate><title>A rationally optimised batch bioreactor cultivation of Viola odorata plant cells for sustainable production of its key bioactive principles</title><author>Babu, R. ; Srivastava, Smita</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c233t-2ff987904dbaa61c21e4cc7946f6a39348ff89cb0a8359c1c776b9a408d935133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>adulterated products</topic><topic>Antibacterial activity</topic><topic>antibacterial properties</topic><topic>antioxidant activity</topic><topic>Biological activity</topic><topic>Biomass</topic><topic>biomass production</topic><topic>Biomedical and Life Sciences</topic><topic>bioprocessing</topic><topic>Bioreactors</topic><topic>Cell culture</topic><topic>Cultivation</topic><topic>Culture</topic><topic>Gas chromatography</topic><topic>Inoculum</topic><topic>inoculum density</topic><topic>Life Sciences</topic><topic>Light intensity</topic><topic>Luminous intensity</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medicinal plants</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Original Article</topic><topic>phytomass</topic><topic>Plant biomass</topic><topic>Plant cells</topic><topic>Plant diversity</topic><topic>Plant extracts</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Productivity</topic><topic>Raw materials</topic><topic>Respiratory diseases</topic><topic>respiratory system</topic><topic>Respiratory tract</topic><topic>Respiratory tract infection</topic><topic>Shaking</topic><topic>species diversity</topic><topic>Sustainable development</topic><topic>Sustainable production</topic><topic>temperature</topic><topic>traditional medicine</topic><topic>Viola odorata</topic><topic>Vitamin E</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Babu, R.</creatorcontrib><creatorcontrib>Srivastava, Smita</creatorcontrib><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Babu, R.</au><au>Srivastava, Smita</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rationally optimised batch bioreactor cultivation of Viola odorata plant cells for sustainable production of its key bioactive principles</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2024-08-01</date><risdate>2024</risdate><volume>158</volume><issue>2</issue><spage>35</spage><epage>35</epage><pages>35-35</pages><artnum>35</artnum><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>Viola odorata
plant extracts are extensively used in indigenous medicine to treat respiratory diseases.
V. odorata’s
limited availability in nature, due to geographical conditions and adulteration, makes continuous supply of high-quality plant raw material challenging. This results in low-quality plant extracts that can have inconsistent and sometimes adverse effects when used in medicine. As an effective solution, in vitro plant biomass cultivation conserves plant diversity, ensuring high-quality raw materials with uniform quality and quantity. Toward this, the current study focuses on establishing bioprocess strategy to achieve maximum.
V. odorata
biomass productivity up to bioreactor level. In this context, in vitro culture conditions were rationally optimised, focusing upon both statistical optimization and one-factor-at-a-time (OFAT) approach, for improved biomass productivity. Inoculum density, macronutrient concentration, shaking speed, temperature, pH and light intensity were systematically optimized to achieve the maximum biomass. This led to increased biomass production from 10.2 ± 0.8 g DW L
− 1
to 21.7 ± 0.8 g DW L
− 1
within 12 days of the batch cultivation period. Optimized culture conditions were implemented in three different types of bioreactors and cultivation in a stirred tank reactor produced ∼ 19.7 g DW L
− 1
of maximum biomass within a 12 day batch cultivation period. Further, the metabolite profile of the bioreactor cultivated biomass (alcoholic extract) was compared with that of the natural plant biomass using gas chromatography-mass spectrometry-based metabolomics. The antibacterial activity of the plant extract was tested against three bacteria responsible for respiratory tract infections, and their antioxidant activity was compared with that of Vitamin E, wherein the results demonstrated comparable activity. The novelty of this study is in the fact that the
V. odorata
plant cells were cultivated in bioreactors for the first time for potential medicinal applications. It provides an alternative method for sustainable production of plant biomass, thereby conserving the natural plant sources.
Graphical Abstract
Key message
Bioreactor cultivation of
V. odorata
plant cells with biomass productivity equivalent to that in shake flask culture, yielding extracts with a similar metabolite profile and associated bioactivity to that in the natural plant extracts.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-024-02829-y</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-9582-3904</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-6857 |
| ispartof | Plant cell, tissue and organ culture, 2024-08, Vol.158 (2), p.35-35, Article 35 |
| issn | 0167-6857 1573-5044 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3153709734 |
| source | Springer Nature - Complete Springer Journals |
| subjects | adulterated products Antibacterial activity antibacterial properties antioxidant activity Biological activity Biomass biomass production Biomedical and Life Sciences bioprocessing Bioreactors Cell culture Cultivation Culture Gas chromatography Inoculum inoculum density Life Sciences Light intensity Luminous intensity Mass spectrometry Mass spectroscopy Medicinal plants Metabolites Metabolomics Original Article phytomass Plant biomass Plant cells Plant diversity Plant extracts Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Productivity Raw materials Respiratory diseases respiratory system Respiratory tract Respiratory tract infection Shaking species diversity Sustainable development Sustainable production temperature traditional medicine Viola odorata Vitamin E |
| title | A rationally optimised batch bioreactor cultivation of Viola odorata plant cells for sustainable production of its key bioactive principles |
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