A reverse transcription loop-mediated isothermal amplification assay for the detection of citrus exocortis viroid in Australian citrus
Citrus exocortis viroid (CEVd), the causal agent of exocortis, is a pathogen that is thought to infect all citrus varieties, although it is asymptomatic in most. Symptoms of exocortis develop on susceptible rootstocks, resulting in stunting and yield reduction. To aid the detection and management of...
Gespeichert in:
| Veröffentlicht in: | Australasian plant pathology 2023-03, Vol.52 (2), p.121-132 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 132 |
|---|---|
| container_issue | 2 |
| container_start_page | 121 |
| container_title | Australasian plant pathology |
| container_volume | 52 |
| creator | Chambers, Grant A Geering, Andrew DW Holford, Paul Kehoe, Monica A Vidalakis, Georgios Donovan, Nerida J |
| description | Citrus exocortis viroid (CEVd), the causal agent of exocortis, is a pathogen that is thought to infect all citrus varieties, although it is asymptomatic in most. Symptoms of exocortis develop on susceptible rootstocks, resulting in stunting and yield reduction. To aid the detection and management of CEVd, a rapid near-field assay was developed using reverse transcription loop-mediated isothermal amplification (RT-LAMP) for the detection of the viroids in nursery and field trees. Over 240 CEVd sequences, including sequence variants from representative Australian isolates that induce mild and severe symptoms, were used in the design of the primers. The RT-LAMP successfully detected CEVd in a 1:1000 dilution (236 pg) of plant total RNA indicating high sensitivity, and also detected the viroid in rapid, crude plant extractions. The assay was highly specific to CEVd, given there was no cross-reactivity with other citrus-infecting pathogens. This new assay provides a simple, robust, specific, and sensitive method to detect CEVd in Australian citrus and to our knowledge, is the first RT-LAMP assay to detect any citrus-infecting viroid. |
| doi_str_mv | 10.1007/s13313-023-00903-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153708695</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2898627616</sourcerecordid><originalsourceid>FETCH-LOGICAL-c396t-b39344bbe2fda2adbfad7beacbf53112775c6aa7d140fe4c11e962f2f5006c483</originalsourceid><addsrcrecordid>eNp9kcFq3DAQhkVpoNukL9CToJdenGgkW7aPS0iTQCCX9ixkedRqsS1XI4fmBfLcVXcDhR56GAZmvv-fgZ-xjyAuQYj2ikApUJWQpUQvVAVv2A7ququ0UPIt24kOmkpBD-_Ye6KDEFBrJXbsZc8TPmEi5DnZhVwKaw5x4VOMazXjGGzGkQeK-Qem2U7czusUfHD2iFki-8x9TLzs-YgZ3XEePXchp404_oouphyIP4UUQ_Fa-H6jcm0KdnmlLtiZtxPhh9d-zr59ufl6fVc9PN7eX-8fKqd6natB9aquhwGlH6204-Dt2A5o3eAbBSDbtnHa2naEWnisHQD2WnrpGyG0qzt1zj6ffNcUf25I2cyBHE6TXTBuZBQ0qhWd7puCfvoHPcQtLeU7I7u-07LVoAslT5RLkSihN2sKs03PBoT5E405RWNKNOYYjYEiUicRFXj5jumv9X9UvwGoxpUS</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2898627616</pqid></control><display><type>article</type><title>A reverse transcription loop-mediated isothermal amplification assay for the detection of citrus exocortis viroid in Australian citrus</title><source>SpringerLink Journals - AutoHoldings</source><creator>Chambers, Grant A ; Geering, Andrew DW ; Holford, Paul ; Kehoe, Monica A ; Vidalakis, Georgios ; Donovan, Nerida J</creator><creatorcontrib>Chambers, Grant A ; Geering, Andrew DW ; Holford, Paul ; Kehoe, Monica A ; Vidalakis, Georgios ; Donovan, Nerida J</creatorcontrib><description>Citrus exocortis viroid (CEVd), the causal agent of exocortis, is a pathogen that is thought to infect all citrus varieties, although it is asymptomatic in most. Symptoms of exocortis develop on susceptible rootstocks, resulting in stunting and yield reduction. To aid the detection and management of CEVd, a rapid near-field assay was developed using reverse transcription loop-mediated isothermal amplification (RT-LAMP) for the detection of the viroids in nursery and field trees. Over 240 CEVd sequences, including sequence variants from representative Australian isolates that induce mild and severe symptoms, were used in the design of the primers. The RT-LAMP successfully detected CEVd in a 1:1000 dilution (236 pg) of plant total RNA indicating high sensitivity, and also detected the viroid in rapid, crude plant extractions. The assay was highly specific to CEVd, given there was no cross-reactivity with other citrus-infecting pathogens. This new assay provides a simple, robust, specific, and sensitive method to detect CEVd in Australian citrus and to our knowledge, is the first RT-LAMP assay to detect any citrus-infecting viroid.</description><identifier>ISSN: 0815-3191</identifier><identifier>EISSN: 1448-6032</identifier><identifier>DOI: 10.1007/s13313-023-00903-1</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Assaying ; Biomedical and Life Sciences ; Citrus ; Citrus exocortis viroid ; cross reaction ; Cross-reactivity ; Dilution ; Ecology ; Entomology ; Fruits ; Gene amplification ; Life Sciences ; Original Research Article ; Pathogens ; Plant extracts ; Plant Pathology ; Plant Sciences ; Reverse transcription ; reverse transcription loop-mediated isothermal amplification ; RNA ; Viroids</subject><ispartof>Australasian plant pathology, 2023-03, Vol.52 (2), p.121-132</ispartof><rights>The Author(s) 2023</rights><rights>The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-b39344bbe2fda2adbfad7beacbf53112775c6aa7d140fe4c11e962f2f5006c483</citedby><cites>FETCH-LOGICAL-c396t-b39344bbe2fda2adbfad7beacbf53112775c6aa7d140fe4c11e962f2f5006c483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13313-023-00903-1$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13313-023-00903-1$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Chambers, Grant A</creatorcontrib><creatorcontrib>Geering, Andrew DW</creatorcontrib><creatorcontrib>Holford, Paul</creatorcontrib><creatorcontrib>Kehoe, Monica A</creatorcontrib><creatorcontrib>Vidalakis, Georgios</creatorcontrib><creatorcontrib>Donovan, Nerida J</creatorcontrib><title>A reverse transcription loop-mediated isothermal amplification assay for the detection of citrus exocortis viroid in Australian citrus</title><title>Australasian plant pathology</title><addtitle>Australasian Plant Pathol</addtitle><description>Citrus exocortis viroid (CEVd), the causal agent of exocortis, is a pathogen that is thought to infect all citrus varieties, although it is asymptomatic in most. Symptoms of exocortis develop on susceptible rootstocks, resulting in stunting and yield reduction. To aid the detection and management of CEVd, a rapid near-field assay was developed using reverse transcription loop-mediated isothermal amplification (RT-LAMP) for the detection of the viroids in nursery and field trees. Over 240 CEVd sequences, including sequence variants from representative Australian isolates that induce mild and severe symptoms, were used in the design of the primers. The RT-LAMP successfully detected CEVd in a 1:1000 dilution (236 pg) of plant total RNA indicating high sensitivity, and also detected the viroid in rapid, crude plant extractions. The assay was highly specific to CEVd, given there was no cross-reactivity with other citrus-infecting pathogens. This new assay provides a simple, robust, specific, and sensitive method to detect CEVd in Australian citrus and to our knowledge, is the first RT-LAMP assay to detect any citrus-infecting viroid.</description><subject>Agriculture</subject><subject>Assaying</subject><subject>Biomedical and Life Sciences</subject><subject>Citrus</subject><subject>Citrus exocortis viroid</subject><subject>cross reaction</subject><subject>Cross-reactivity</subject><subject>Dilution</subject><subject>Ecology</subject><subject>Entomology</subject><subject>Fruits</subject><subject>Gene amplification</subject><subject>Life Sciences</subject><subject>Original Research Article</subject><subject>Pathogens</subject><subject>Plant extracts</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Reverse transcription</subject><subject>reverse transcription loop-mediated isothermal amplification</subject><subject>RNA</subject><subject>Viroids</subject><issn>0815-3191</issn><issn>1448-6032</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kcFq3DAQhkVpoNukL9CToJdenGgkW7aPS0iTQCCX9ixkedRqsS1XI4fmBfLcVXcDhR56GAZmvv-fgZ-xjyAuQYj2ikApUJWQpUQvVAVv2A7ququ0UPIt24kOmkpBD-_Ye6KDEFBrJXbsZc8TPmEi5DnZhVwKaw5x4VOMazXjGGzGkQeK-Qem2U7czusUfHD2iFki-8x9TLzs-YgZ3XEePXchp404_oouphyIP4UUQ_Fa-H6jcm0KdnmlLtiZtxPhh9d-zr59ufl6fVc9PN7eX-8fKqd6natB9aquhwGlH6204-Dt2A5o3eAbBSDbtnHa2naEWnisHQD2WnrpGyG0qzt1zj6ffNcUf25I2cyBHE6TXTBuZBQ0qhWd7puCfvoHPcQtLeU7I7u-07LVoAslT5RLkSihN2sKs03PBoT5E405RWNKNOYYjYEiUicRFXj5jumv9X9UvwGoxpUS</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Chambers, Grant A</creator><creator>Geering, Andrew DW</creator><creator>Holford, Paul</creator><creator>Kehoe, Monica A</creator><creator>Vidalakis, Georgios</creator><creator>Donovan, Nerida J</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20230301</creationdate><title>A reverse transcription loop-mediated isothermal amplification assay for the detection of citrus exocortis viroid in Australian citrus</title><author>Chambers, Grant A ; Geering, Andrew DW ; Holford, Paul ; Kehoe, Monica A ; Vidalakis, Georgios ; Donovan, Nerida J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-b39344bbe2fda2adbfad7beacbf53112775c6aa7d140fe4c11e962f2f5006c483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Agriculture</topic><topic>Assaying</topic><topic>Biomedical and Life Sciences</topic><topic>Citrus</topic><topic>Citrus exocortis viroid</topic><topic>cross reaction</topic><topic>Cross-reactivity</topic><topic>Dilution</topic><topic>Ecology</topic><topic>Entomology</topic><topic>Fruits</topic><topic>Gene amplification</topic><topic>Life Sciences</topic><topic>Original Research Article</topic><topic>Pathogens</topic><topic>Plant extracts</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Reverse transcription</topic><topic>reverse transcription loop-mediated isothermal amplification</topic><topic>RNA</topic><topic>Viroids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chambers, Grant A</creatorcontrib><creatorcontrib>Geering, Andrew DW</creatorcontrib><creatorcontrib>Holford, Paul</creatorcontrib><creatorcontrib>Kehoe, Monica A</creatorcontrib><creatorcontrib>Vidalakis, Georgios</creatorcontrib><creatorcontrib>Donovan, Nerida J</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Australasian plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chambers, Grant A</au><au>Geering, Andrew DW</au><au>Holford, Paul</au><au>Kehoe, Monica A</au><au>Vidalakis, Georgios</au><au>Donovan, Nerida J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A reverse transcription loop-mediated isothermal amplification assay for the detection of citrus exocortis viroid in Australian citrus</atitle><jtitle>Australasian plant pathology</jtitle><stitle>Australasian Plant Pathol</stitle><date>2023-03-01</date><risdate>2023</risdate><volume>52</volume><issue>2</issue><spage>121</spage><epage>132</epage><pages>121-132</pages><issn>0815-3191</issn><eissn>1448-6032</eissn><abstract>Citrus exocortis viroid (CEVd), the causal agent of exocortis, is a pathogen that is thought to infect all citrus varieties, although it is asymptomatic in most. Symptoms of exocortis develop on susceptible rootstocks, resulting in stunting and yield reduction. To aid the detection and management of CEVd, a rapid near-field assay was developed using reverse transcription loop-mediated isothermal amplification (RT-LAMP) for the detection of the viroids in nursery and field trees. Over 240 CEVd sequences, including sequence variants from representative Australian isolates that induce mild and severe symptoms, were used in the design of the primers. The RT-LAMP successfully detected CEVd in a 1:1000 dilution (236 pg) of plant total RNA indicating high sensitivity, and also detected the viroid in rapid, crude plant extractions. The assay was highly specific to CEVd, given there was no cross-reactivity with other citrus-infecting pathogens. This new assay provides a simple, robust, specific, and sensitive method to detect CEVd in Australian citrus and to our knowledge, is the first RT-LAMP assay to detect any citrus-infecting viroid.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s13313-023-00903-1</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0815-3191 |
| ispartof | Australasian plant pathology, 2023-03, Vol.52 (2), p.121-132 |
| issn | 0815-3191 1448-6032 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3153708695 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Assaying Biomedical and Life Sciences Citrus Citrus exocortis viroid cross reaction Cross-reactivity Dilution Ecology Entomology Fruits Gene amplification Life Sciences Original Research Article Pathogens Plant extracts Plant Pathology Plant Sciences Reverse transcription reverse transcription loop-mediated isothermal amplification RNA Viroids |
| title | A reverse transcription loop-mediated isothermal amplification assay for the detection of citrus exocortis viroid in Australian citrus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T23%3A46%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20reverse%20transcription%20loop-mediated%20isothermal%20amplification%20assay%20for%20the%20detection%20of%20citrus%20exocortis%20viroid%20in%20Australian%20citrus&rft.jtitle=Australasian%20plant%20pathology&rft.au=Chambers,%20Grant%20A&rft.date=2023-03-01&rft.volume=52&rft.issue=2&rft.spage=121&rft.epage=132&rft.pages=121-132&rft.issn=0815-3191&rft.eissn=1448-6032&rft_id=info:doi/10.1007/s13313-023-00903-1&rft_dat=%3Cproquest_cross%3E2898627616%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2898627616&rft_id=info:pmid/&rfr_iscdi=true |