Development of a Novel Sulfur Quantum Dots: Synthesis, 99mTc Radiolabeling, and Biodistribution
Sulfur quantum dots (SQDs) as free heavy metal element quantum dots have promising applications in diagnosis and therapy; however, SQDs’ in vivo biodistribution has not been studied. In the current study, SQDs were synthesized directly from cheap sublimated sulfur powder via a one-pot solvothermal m...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2024-06, Vol.196 (6), p.3356-3373 |
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| creator | Mirzaei, Seyedeh Mozhdeh Oskuee, Reza Kazemi Sadri, Kayvan Sabouri, Zahra Far, Bahareh Farasati Abdulabbas, Hadi Sajid Darroudi, Majid |
| description | Sulfur quantum dots (SQDs) as free heavy metal element quantum dots have promising applications in diagnosis and therapy; however, SQDs’ in vivo biodistribution has not been studied. In the current study, SQDs were synthesized directly from cheap sublimated sulfur powder via a one-pot solvothermal method, and sucrose was used as a stabilizer to enhance stability and biocompatibility. The as-obtained SQDs with an average size of 4.6 nm exhibited great water dispersity, highly favorable quantum yield (21.5%), and uniformly spherical shape which were confirmed by UV-Vis, fluorescence spectrophotometer, TEM, and FESEM/EDX/PSA analyses. Moreover, the as-synthesized SQDs had very low cytotoxicity based on cancer (C26) and normal (L929) cell lines via MTT assay. And also, SQDs were radio-labeled directly by Technetium-99m (
99m
Tc), which had good stability ranging from 86 to 99% in PBS and human serum. The SQDs’ cell uptake on C26 and L929 cell lines demonstrated that cancer cells had more uptake than normal cells by increasing concentrations. Moreover, SQDs’ in vivo biodistribution results displayed high kidney dose accumulation and rapid renal clearance, making them suitable for imaging and therapeutic applications.
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| doi_str_mv | 10.1007/s12010-023-04703-7 |
| format | Article |
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99m
Tc), which had good stability ranging from 86 to 99% in PBS and human serum. The SQDs’ cell uptake on C26 and L929 cell lines demonstrated that cancer cells had more uptake than normal cells by increasing concentrations. Moreover, SQDs’ in vivo biodistribution results displayed high kidney dose accumulation and rapid renal clearance, making them suitable for imaging and therapeutic applications.
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99m
Tc), which had good stability ranging from 86 to 99% in PBS and human serum. The SQDs’ cell uptake on C26 and L929 cell lines demonstrated that cancer cells had more uptake than normal cells by increasing concentrations. Moreover, SQDs’ in vivo biodistribution results displayed high kidney dose accumulation and rapid renal clearance, making them suitable for imaging and therapeutic applications.
Graphical Abstract</description><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Biodistribution</subject><subject>Biotechnology</subject><subject>blood serum</subject><subject>Cancer</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cytotoxicity</subject><subject>fluorescence emission spectroscopy</subject><subject>Heavy metals</subject><subject>humans</subject><subject>In vivo methods and tests</subject><subject>kidneys</subject><subject>Original Article</subject><subject>Quantum dots</subject><subject>radiolabeling</subject><subject>Radiolabelling</subject><subject>renal clearance</subject><subject>Stability</subject><subject>stabilizers</subject><subject>Sucrose</subject><subject>Sulfur</subject><subject>Synthesis</subject><subject>Technetium</subject><subject>Technetium isotopes</subject><subject>Therapeutic applications</subject><subject>therapeutics</subject><subject>toxicity testing</subject><subject>Tumor cell lines</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkUFP3DAQhS1UJLaUP8DJEpceCB3bsR331u5SioRAwHK2HMdZjBJ7aydI_PsatlKlHuhppKfvvdHMQ-iYwBkBkF8yoUCgAsoqqCWwSu6hBeFcFUmRD2gBVLKK0kYdoI85PwEQ2nC5QHrlnt0Qt6MLE449Nvg6FgHfz0M_J3w7mzDNI17FKX_F9y9henTZ51Os1Li2-M50Pg6mdYMPm1NsQoe_-9j5PCXfzpOP4RPa782Q3dGfeYgefpyvlz-rq5uLy-W3q8oywqeqBVEr03Q9a1hjKJUtpZa3teiaTkouCmV5TynwXlnmOiF4rWw5oq-NYVawQ_R5l7tN8dfs8qRHn60bBhNcnLMuW5hogNb_R8tjlABKJCnoyT_oU5xTKIdoBkIIaGr2Gkh3lE0x5-R6vU1-NOlFE9Cv9ehdPbrUo9_q0bKY2M6UCxw2Lv2Nfsf1G_RCkJU</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Mirzaei, Seyedeh Mozhdeh</creator><creator>Oskuee, Reza Kazemi</creator><creator>Sadri, Kayvan</creator><creator>Sabouri, Zahra</creator><creator>Far, Bahareh Farasati</creator><creator>Abdulabbas, Hadi Sajid</creator><creator>Darroudi, Majid</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-2624-7242</orcidid></search><sort><creationdate>20240601</creationdate><title>Development of a Novel Sulfur Quantum Dots: Synthesis, 99mTc Radiolabeling, and Biodistribution</title><author>Mirzaei, Seyedeh Mozhdeh ; 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however, SQDs’ in vivo biodistribution has not been studied. In the current study, SQDs were synthesized directly from cheap sublimated sulfur powder via a one-pot solvothermal method, and sucrose was used as a stabilizer to enhance stability and biocompatibility. The as-obtained SQDs with an average size of 4.6 nm exhibited great water dispersity, highly favorable quantum yield (21.5%), and uniformly spherical shape which were confirmed by UV-Vis, fluorescence spectrophotometer, TEM, and FESEM/EDX/PSA analyses. Moreover, the as-synthesized SQDs had very low cytotoxicity based on cancer (C26) and normal (L929) cell lines via MTT assay. And also, SQDs were radio-labeled directly by Technetium-99m (
99m
Tc), which had good stability ranging from 86 to 99% in PBS and human serum. The SQDs’ cell uptake on C26 and L929 cell lines demonstrated that cancer cells had more uptake than normal cells by increasing concentrations. Moreover, SQDs’ in vivo biodistribution results displayed high kidney dose accumulation and rapid renal clearance, making them suitable for imaging and therapeutic applications.
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| subjects | Biochemistry Biocompatibility Biodistribution Biotechnology blood serum Cancer Chemistry Chemistry and Materials Science Cytotoxicity fluorescence emission spectroscopy Heavy metals humans In vivo methods and tests kidneys Original Article Quantum dots radiolabeling Radiolabelling renal clearance Stability stabilizers Sucrose Sulfur Synthesis Technetium Technetium isotopes Therapeutic applications therapeutics toxicity testing Tumor cell lines |
| title | Development of a Novel Sulfur Quantum Dots: Synthesis, 99mTc Radiolabeling, and Biodistribution |
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