Alternative system for micropropagation of Prunus campanulata (Maxim.)
Herein, an in vitro multiplication protocol for Prunus campanulata was evaluated. The effects of 15.0, 30.0, and 45.0 g L −1 sucrose and three flask sealing methods (PVC film, rigid polypropylene lid, and rigid lid with membrane) were analyzed at the multiplication, rooting, and acclimatization stag...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2024-06, Vol.60 (3), p.333-343 |
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| creator | Sanson, Daniela de Almeida Garrett, Alexandre Techy Rodrigues, Isy Cavalhães de Magalhães Miranda, Gabriel de Ávila, Fabricio William Fogaça, Cristiane Alves Gasparin, Ezequiel Bobrowski, Rogério Peres, Fabiana Schmidt Bandeira |
| description | Herein, an
in vitro
multiplication protocol for
Prunus campanulata
was evaluated. The effects of 15.0, 30.0, and 45.0 g L
−1
sucrose and three flask sealing methods (PVC film, rigid polypropylene lid, and rigid lid with membrane) were analyzed at the multiplication, rooting, and acclimatization stages. For
in vitro
multiplication, shoot tips of approximately 1 cm in length containing two pairs of leaves from seedlings germinated
in vitro
in Woody Plant Medium (WPM) were used. Evaluations considered the multiplication of shoot apexes in three subcultures (at 40, 80, and 120 d) for survival, number of shoots, shoot length, number of leaves, and multiplication rate. In the rooting phase, the rooting percentage, number of roots, and root length were assessed. In the acclimatization phase under shaded and full sun conditions, seedling survival and biometric characteristics were evaluated, including stem diameter, height, and number of leaves. The addition of sucrose to the medium, at a concentration of 45.0 g L
−1
, associated with sealing using PVC plastic film, showed inadequate results for
in vitro
multiplication of
P. campanulata
. The addition of sucrose to the culture medium in concentrations ranging from 15.0 to 30.0 g L
−1
favored
in vitro
rooting. The use of a permeable membrane for sealing enabled the growth of more vigorous shoots in terms of percentage of rooting and root length, and these seedlings stood out in the acclimatization phases with greater rates of survival and improved biometric characteristics. |
| doi_str_mv | 10.1007/s11627-024-10424-1 |
| format | Article |
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in vitro
multiplication protocol for
Prunus campanulata
was evaluated. The effects of 15.0, 30.0, and 45.0 g L
−1
sucrose and three flask sealing methods (PVC film, rigid polypropylene lid, and rigid lid with membrane) were analyzed at the multiplication, rooting, and acclimatization stages. For
in vitro
multiplication, shoot tips of approximately 1 cm in length containing two pairs of leaves from seedlings germinated
in vitro
in Woody Plant Medium (WPM) were used. Evaluations considered the multiplication of shoot apexes in three subcultures (at 40, 80, and 120 d) for survival, number of shoots, shoot length, number of leaves, and multiplication rate. In the rooting phase, the rooting percentage, number of roots, and root length were assessed. In the acclimatization phase under shaded and full sun conditions, seedling survival and biometric characteristics were evaluated, including stem diameter, height, and number of leaves. The addition of sucrose to the medium, at a concentration of 45.0 g L
−1
, associated with sealing using PVC plastic film, showed inadequate results for
in vitro
multiplication of
P. campanulata
. The addition of sucrose to the culture medium in concentrations ranging from 15.0 to 30.0 g L
−1
favored
in vitro
rooting. The use of a permeable membrane for sealing enabled the growth of more vigorous shoots in terms of percentage of rooting and root length, and these seedlings stood out in the acclimatization phases with greater rates of survival and improved biometric characteristics.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/s11627-024-10424-1</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>acclimation ; Acclimatization ; Biomedical and Life Sciences ; Biometrics ; biometry ; Cell Biology ; culture media ; Developmental Biology ; Leaves ; Life Sciences ; Membranes ; Micropropagation ; Morphology ; Multiplication ; Physiology ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Plant growth ; Plant Sciences ; Plant Tissue Culture ; plastic film ; Polypropylene ; polypropylenes ; Polyvinyl chloride ; Prunus campanulata ; Rooting ; Sealing ; Seedlings ; Shoots ; Sucrose ; Survival ; Woody plants</subject><ispartof>In vitro cellular & developmental biology. Plant, 2024-06, Vol.60 (3), p.333-343</ispartof><rights>The Society for In Vitro Biology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>Copyright Springer Nature B.V. Jul 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c303t-b38d231150c7769ce9c4ee8c917e73753325250873bb7a7de8fd0321368773893</cites><orcidid>0000-0003-4834-1667</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11627-024-10424-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11627-024-10424-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Sanson, Daniela</creatorcontrib><creatorcontrib>de Almeida Garrett, Alexandre Techy</creatorcontrib><creatorcontrib>Rodrigues, Isy Cavalhães</creatorcontrib><creatorcontrib>de Magalhães Miranda, Gabriel</creatorcontrib><creatorcontrib>de Ávila, Fabricio William</creatorcontrib><creatorcontrib>Fogaça, Cristiane Alves</creatorcontrib><creatorcontrib>Gasparin, Ezequiel</creatorcontrib><creatorcontrib>Bobrowski, Rogério</creatorcontrib><creatorcontrib>Peres, Fabiana Schmidt Bandeira</creatorcontrib><title>Alternative system for micropropagation of Prunus campanulata (Maxim.)</title><title>In vitro cellular & developmental biology. Plant</title><addtitle>In Vitro Cell.Dev.Biol.-Plant</addtitle><description>Herein, an
in vitro
multiplication protocol for
Prunus campanulata
was evaluated. The effects of 15.0, 30.0, and 45.0 g L
−1
sucrose and three flask sealing methods (PVC film, rigid polypropylene lid, and rigid lid with membrane) were analyzed at the multiplication, rooting, and acclimatization stages. For
in vitro
multiplication, shoot tips of approximately 1 cm in length containing two pairs of leaves from seedlings germinated
in vitro
in Woody Plant Medium (WPM) were used. Evaluations considered the multiplication of shoot apexes in three subcultures (at 40, 80, and 120 d) for survival, number of shoots, shoot length, number of leaves, and multiplication rate. In the rooting phase, the rooting percentage, number of roots, and root length were assessed. In the acclimatization phase under shaded and full sun conditions, seedling survival and biometric characteristics were evaluated, including stem diameter, height, and number of leaves. The addition of sucrose to the medium, at a concentration of 45.0 g L
−1
, associated with sealing using PVC plastic film, showed inadequate results for
in vitro
multiplication of
P. campanulata
. The addition of sucrose to the culture medium in concentrations ranging from 15.0 to 30.0 g L
−1
favored
in vitro
rooting. The use of a permeable membrane for sealing enabled the growth of more vigorous shoots in terms of percentage of rooting and root length, and these seedlings stood out in the acclimatization phases with greater rates of survival and improved biometric characteristics.</description><subject>acclimation</subject><subject>Acclimatization</subject><subject>Biomedical and Life Sciences</subject><subject>Biometrics</subject><subject>biometry</subject><subject>Cell Biology</subject><subject>culture media</subject><subject>Developmental Biology</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Membranes</subject><subject>Micropropagation</subject><subject>Morphology</subject><subject>Multiplication</subject><subject>Physiology</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant growth</subject><subject>Plant Sciences</subject><subject>Plant Tissue Culture</subject><subject>plastic film</subject><subject>Polypropylene</subject><subject>polypropylenes</subject><subject>Polyvinyl chloride</subject><subject>Prunus campanulata</subject><subject>Rooting</subject><subject>Sealing</subject><subject>Seedlings</subject><subject>Shoots</subject><subject>Sucrose</subject><subject>Survival</subject><subject>Woody plants</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEQhoMoWKt_wNOCl3rYOslsdrLHUqwKih70HNI0W7bsR012xf57UysIHoRhZmCed5h5GbvkMOUAdBM4zwWlILKUQ7bPR2zEM5KpyFVxHHuQWSozyk_ZWQgbAODAacQWs7p3vjV99eGSsAu9a5Ky80lTWd9tY5h1nHVt0pXJix_aISTWNFvTDrXpTTJ5Mp9VM70-ZyelqYO7-Klj9ra4fZ3fp4_Pdw_z2WNqEbBPl6hWAjmXYInywrrCZs4pW3ByhCQRhRQSFOFySYZWTpUrQMExV0SoChyzyWFvPO19cKHXTRWsq2vTum4IGrnEnBQpjOjVH3TTDfHTOlKgUGSSk4iUOFDx3RC8K_XWV43xO81B763VB2t1tFZ_W6t5FOFBFCLcrp3_Xf2P6gsjlHm6</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Sanson, Daniela</creator><creator>de Almeida Garrett, Alexandre Techy</creator><creator>Rodrigues, Isy Cavalhães</creator><creator>de Magalhães Miranda, Gabriel</creator><creator>de Ávila, Fabricio William</creator><creator>Fogaça, Cristiane Alves</creator><creator>Gasparin, Ezequiel</creator><creator>Bobrowski, Rogério</creator><creator>Peres, Fabiana Schmidt Bandeira</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>4T-</scope><scope>4U-</scope><scope>K9.</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0003-4834-1667</orcidid></search><sort><creationdate>20240601</creationdate><title>Alternative system for micropropagation of Prunus campanulata (Maxim.)</title><author>Sanson, Daniela ; de Almeida Garrett, Alexandre Techy ; Rodrigues, Isy Cavalhães ; de Magalhães Miranda, Gabriel ; de Ávila, Fabricio William ; Fogaça, Cristiane Alves ; Gasparin, Ezequiel ; Bobrowski, Rogério ; Peres, Fabiana Schmidt Bandeira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-b38d231150c7769ce9c4ee8c917e73753325250873bb7a7de8fd0321368773893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>acclimation</topic><topic>Acclimatization</topic><topic>Biomedical and Life Sciences</topic><topic>Biometrics</topic><topic>biometry</topic><topic>Cell Biology</topic><topic>culture media</topic><topic>Developmental Biology</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Membranes</topic><topic>Micropropagation</topic><topic>Morphology</topic><topic>Multiplication</topic><topic>Physiology</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant growth</topic><topic>Plant Sciences</topic><topic>Plant Tissue Culture</topic><topic>plastic film</topic><topic>Polypropylene</topic><topic>polypropylenes</topic><topic>Polyvinyl chloride</topic><topic>Prunus campanulata</topic><topic>Rooting</topic><topic>Sealing</topic><topic>Seedlings</topic><topic>Shoots</topic><topic>Sucrose</topic><topic>Survival</topic><topic>Woody plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sanson, Daniela</creatorcontrib><creatorcontrib>de Almeida Garrett, Alexandre Techy</creatorcontrib><creatorcontrib>Rodrigues, Isy Cavalhães</creatorcontrib><creatorcontrib>de Magalhães Miranda, Gabriel</creatorcontrib><creatorcontrib>de Ávila, Fabricio William</creatorcontrib><creatorcontrib>Fogaça, Cristiane Alves</creatorcontrib><creatorcontrib>Gasparin, Ezequiel</creatorcontrib><creatorcontrib>Bobrowski, Rogério</creatorcontrib><creatorcontrib>Peres, Fabiana Schmidt Bandeira</creatorcontrib><collection>CrossRef</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sanson, Daniela</au><au>de Almeida Garrett, Alexandre Techy</au><au>Rodrigues, Isy Cavalhães</au><au>de Magalhães Miranda, Gabriel</au><au>de Ávila, Fabricio William</au><au>Fogaça, Cristiane Alves</au><au>Gasparin, Ezequiel</au><au>Bobrowski, Rogério</au><au>Peres, Fabiana Schmidt Bandeira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alternative system for micropropagation of Prunus campanulata (Maxim.)</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><stitle>In Vitro Cell.Dev.Biol.-Plant</stitle><date>2024-06-01</date><risdate>2024</risdate><volume>60</volume><issue>3</issue><spage>333</spage><epage>343</epage><pages>333-343</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>Herein, an
in vitro
multiplication protocol for
Prunus campanulata
was evaluated. The effects of 15.0, 30.0, and 45.0 g L
−1
sucrose and three flask sealing methods (PVC film, rigid polypropylene lid, and rigid lid with membrane) were analyzed at the multiplication, rooting, and acclimatization stages. For
in vitro
multiplication, shoot tips of approximately 1 cm in length containing two pairs of leaves from seedlings germinated
in vitro
in Woody Plant Medium (WPM) were used. Evaluations considered the multiplication of shoot apexes in three subcultures (at 40, 80, and 120 d) for survival, number of shoots, shoot length, number of leaves, and multiplication rate. In the rooting phase, the rooting percentage, number of roots, and root length were assessed. In the acclimatization phase under shaded and full sun conditions, seedling survival and biometric characteristics were evaluated, including stem diameter, height, and number of leaves. The addition of sucrose to the medium, at a concentration of 45.0 g L
−1
, associated with sealing using PVC plastic film, showed inadequate results for
in vitro
multiplication of
P. campanulata
. The addition of sucrose to the culture medium in concentrations ranging from 15.0 to 30.0 g L
−1
favored
in vitro
rooting. The use of a permeable membrane for sealing enabled the growth of more vigorous shoots in terms of percentage of rooting and root length, and these seedlings stood out in the acclimatization phases with greater rates of survival and improved biometric characteristics.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s11627-024-10424-1</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-4834-1667</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1054-5476 |
| ispartof | In vitro cellular & developmental biology. Plant, 2024-06, Vol.60 (3), p.333-343 |
| issn | 1054-5476 1475-2689 |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | acclimation Acclimatization Biomedical and Life Sciences Biometrics biometry Cell Biology culture media Developmental Biology Leaves Life Sciences Membranes Micropropagation Morphology Multiplication Physiology Plant Breeding/Biotechnology Plant Genetics and Genomics Plant growth Plant Sciences Plant Tissue Culture plastic film Polypropylene polypropylenes Polyvinyl chloride Prunus campanulata Rooting Sealing Seedlings Shoots Sucrose Survival Woody plants |
| title | Alternative system for micropropagation of Prunus campanulata (Maxim.) |
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