Single-point substitution F97M leads to in cellulo crystallization of the biphotochromic protein moxSAASoti

To enhance the photoconversion performance of biphotochromic moxSAASoti protein, a substitution F97 M was introduced. In addition to enhancing the target properties, this substitution also resulted in the crystallization of the recombinant protein within living HeLa cells (also referred to as in cel...

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Veröffentlicht in:	Biochemical and biophysical research communications 2024-11, Vol.732, p.150419, Article 150419
Hauptverfasser:	Marynich, N.K., Boyko, K.M., Matyuta, I.O., Minyaev, M.E., Khadiyatova, A.A., Popov, V.O., Savitsky, A.P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution crystal structure Crystallization Crystallography, X-Ray fluorescence HeLa Cells Humans Luminescent Proteins - chemistry Luminescent Proteins - genetics Luminescent Proteins - metabolism Models, Molecular Protein Conformation recombinant proteins Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism
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