Evaluation of a field deployable, high-throughput RT-LAMP device as an early warning system for COVID-19 through SARS-CoV-2 measurements in wastewater
Quantification of SARS-CoV-2 RNA copies in wastewater can be used to estimate COVID-19 prevalence in communities. While such results are important for mitigating disease spread, SARS-CoV-2 measurements require sophisticated equipment and trained personnel, for which a centralized laboratory is neces...
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| Veröffentlicht in: | The Science of the total environment 2024-09, Vol.944, p.173744, Article 173744 |
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| creator | Boza, J.M. Amirali, A. Williams, S.L. Currall, B.B. Grills, G.S. Mason, C.E. Solo-Gabriele, H.M. Erickson, D.C. |
| description | Quantification of SARS-CoV-2 RNA copies in wastewater can be used to estimate COVID-19 prevalence in communities. While such results are important for mitigating disease spread, SARS-CoV-2 measurements require sophisticated equipment and trained personnel, for which a centralized laboratory is necessary. This significantly impacts the time to result, defeating its purpose as an early warning detection tool. The objective of this study was to evaluate a field portable device (called MINI) for detecting SARS-CoV-2 viral loads in wastewater using real-time reverse transcriptase loop-mediated isothermal amplification (real-time RT-LAMP). The device was tested using wastewater samples collected from buildings (with 430 to 1430 inhabitants) that had known COVID-19-positive cases. Results show comparable performance of RT-LAMP against reverse transcriptase polymerase chain reaction (RT-qPCR) when detecting SARS-CoV-2 copies in wastewater. Both RT-LAMP and RT-qPCR detected SARS-CoV-2 in wastewater from buildings with at least three positive individuals within a 6-day time frame prior to diagnosis. The large 96-well throughput provided by MINI provided scalability to multi-building detection. The portability of the MINI device enabled decentralized on-site detection, significantly reducing the time to result. The overall findings support the use of RT-LAMP within the MINI configuration as an early detection system for COVID-19 infection using wastewater collected at the building scale.
[Display omitted]
•Decentralized testing of SARS-CoV-2 in wastewater•Wastewater as an early warning system for COVID-19•Wastewater-based surveillance as a tool for the allocation of resources•Real-Time RT-LAMP as an alternative approach to RT-qPCR•Rapid decentralized detection of wastewater |
| doi_str_mv | 10.1016/j.scitotenv.2024.173744 |
| format | Article |
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[Display omitted]
•Decentralized testing of SARS-CoV-2 in wastewater•Wastewater as an early warning system for COVID-19•Wastewater-based surveillance as a tool for the allocation of resources•Real-Time RT-LAMP as an alternative approach to RT-qPCR•Rapid decentralized detection of wastewater</description><identifier>ISSN: 0048-9697</identifier><identifier>ISSN: 1879-1026</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2024.173744</identifier><identifier>PMID: 38844223</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>COVID-19 - diagnosis ; COVID-19 infection ; environment ; human resources ; Humans ; Molecular Diagnostic Techniques - instrumentation ; Molecular Diagnostic Techniques - methods ; Nucleic Acid Amplification Techniques - instrumentation ; Nucleic Acid Amplification Techniques - methods ; portable equipment ; reverse transcriptase polymerase chain reaction ; reverse transcription loop-mediated isothermal amplification ; RNA ; RNA, Viral - analysis ; RT-LAMP ; RT-qPCR ; SARS-CoV-2 ; Severe acute respiratory syndrome coronavirus 2 ; Viral Load ; Wastewater ; Wastewater - virology ; Wastewater-based surveillance</subject><ispartof>The Science of the total environment, 2024-09, Vol.944, p.173744, Article 173744</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c350t-d3ed9f9795111975a41a7d4bbafc700cc99d95057499bd6956fdd8338a24cabe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0048969724038919$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38844223$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boza, J.M.</creatorcontrib><creatorcontrib>Amirali, A.</creatorcontrib><creatorcontrib>Williams, S.L.</creatorcontrib><creatorcontrib>Currall, B.B.</creatorcontrib><creatorcontrib>Grills, G.S.</creatorcontrib><creatorcontrib>Mason, C.E.</creatorcontrib><creatorcontrib>Solo-Gabriele, H.M.</creatorcontrib><creatorcontrib>Erickson, D.C.</creatorcontrib><title>Evaluation of a field deployable, high-throughput RT-LAMP device as an early warning system for COVID-19 through SARS-CoV-2 measurements in wastewater</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>Quantification of SARS-CoV-2 RNA copies in wastewater can be used to estimate COVID-19 prevalence in communities. While such results are important for mitigating disease spread, SARS-CoV-2 measurements require sophisticated equipment and trained personnel, for which a centralized laboratory is necessary. This significantly impacts the time to result, defeating its purpose as an early warning detection tool. The objective of this study was to evaluate a field portable device (called MINI) for detecting SARS-CoV-2 viral loads in wastewater using real-time reverse transcriptase loop-mediated isothermal amplification (real-time RT-LAMP). The device was tested using wastewater samples collected from buildings (with 430 to 1430 inhabitants) that had known COVID-19-positive cases. Results show comparable performance of RT-LAMP against reverse transcriptase polymerase chain reaction (RT-qPCR) when detecting SARS-CoV-2 copies in wastewater. Both RT-LAMP and RT-qPCR detected SARS-CoV-2 in wastewater from buildings with at least three positive individuals within a 6-day time frame prior to diagnosis. The large 96-well throughput provided by MINI provided scalability to multi-building detection. The portability of the MINI device enabled decentralized on-site detection, significantly reducing the time to result. The overall findings support the use of RT-LAMP within the MINI configuration as an early detection system for COVID-19 infection using wastewater collected at the building scale.
[Display omitted]
•Decentralized testing of SARS-CoV-2 in wastewater•Wastewater as an early warning system for COVID-19•Wastewater-based surveillance as a tool for the allocation of resources•Real-Time RT-LAMP as an alternative approach to RT-qPCR•Rapid decentralized detection of wastewater</description><subject>COVID-19 - diagnosis</subject><subject>COVID-19 infection</subject><subject>environment</subject><subject>human resources</subject><subject>Humans</subject><subject>Molecular Diagnostic Techniques - instrumentation</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Nucleic Acid Amplification Techniques - instrumentation</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>portable equipment</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>reverse transcription loop-mediated isothermal amplification</subject><subject>RNA</subject><subject>RNA, Viral - analysis</subject><subject>RT-LAMP</subject><subject>RT-qPCR</subject><subject>SARS-CoV-2</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Viral Load</subject><subject>Wastewater</subject><subject>Wastewater - virology</subject><subject>Wastewater-based surveillance</subject><issn>0048-9697</issn><issn>1879-1026</issn><issn>1879-1026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFuEzEQhi0EoqHwCuAjBxzsXXu9PkZpgUpBRW3p1fLas4mj3XWwvanyIjwvGyX02rnM5fv_keZD6BOjc0ZZ9XU7T9bnkGHYzwta8DmTpeT8FZqxWirCaFG9RjNKeU1UpeQFepfSlk4ja_YWXZR1zXlRlDP093pvutFkHwYcWmxw66Fz2MGuCwfTdPAFb_x6Q_ImhnG92Y0Z3z2Q1eLnr4nZewvYJGwGDCZ2B_xk4uCHNU6HlKHHbYh4eft4c0WYwucGfL-4uyfL8EgK3INJY4QehpywH6b4FHsyGeJ79KY1XYIP532Jfn-7flj-IKvb7zfLxYrYUtBMXAlOtUoqwRhTUhjOjHS8aUxrJaXWKuWUoEJypRpXKVG1ztVlWZuCW9NAeYk-n3p3MfwZIWXd-2Sh68wAYUy6ZKKsJBWieBmllVA1p4JNqDyhNoaUIrR6F31v4kEzqo_-9FY_-9NHf_rkb0p-PB8Zmx7cc-6_sAlYnACYvrL3EI9FMFhwPoLN2gX_4pF_Gyewxw</recordid><startdate>20240920</startdate><enddate>20240920</enddate><creator>Boza, J.M.</creator><creator>Amirali, A.</creator><creator>Williams, S.L.</creator><creator>Currall, B.B.</creator><creator>Grills, G.S.</creator><creator>Mason, C.E.</creator><creator>Solo-Gabriele, H.M.</creator><creator>Erickson, D.C.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240920</creationdate><title>Evaluation of a field deployable, high-throughput RT-LAMP device as an early warning system for COVID-19 through SARS-CoV-2 measurements in wastewater</title><author>Boza, J.M. ; Amirali, A. ; Williams, S.L. ; Currall, B.B. ; Grills, G.S. ; Mason, C.E. ; Solo-Gabriele, H.M. ; Erickson, D.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-d3ed9f9795111975a41a7d4bbafc700cc99d95057499bd6956fdd8338a24cabe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>COVID-19 - diagnosis</topic><topic>COVID-19 infection</topic><topic>environment</topic><topic>human resources</topic><topic>Humans</topic><topic>Molecular Diagnostic Techniques - instrumentation</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Nucleic Acid Amplification Techniques - instrumentation</topic><topic>Nucleic Acid Amplification Techniques - methods</topic><topic>portable equipment</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>reverse transcription loop-mediated isothermal amplification</topic><topic>RNA</topic><topic>RNA, Viral - analysis</topic><topic>RT-LAMP</topic><topic>RT-qPCR</topic><topic>SARS-CoV-2</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Viral Load</topic><topic>Wastewater</topic><topic>Wastewater - virology</topic><topic>Wastewater-based surveillance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boza, J.M.</creatorcontrib><creatorcontrib>Amirali, A.</creatorcontrib><creatorcontrib>Williams, S.L.</creatorcontrib><creatorcontrib>Currall, B.B.</creatorcontrib><creatorcontrib>Grills, G.S.</creatorcontrib><creatorcontrib>Mason, C.E.</creatorcontrib><creatorcontrib>Solo-Gabriele, H.M.</creatorcontrib><creatorcontrib>Erickson, D.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>The Science of the total environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boza, J.M.</au><au>Amirali, A.</au><au>Williams, S.L.</au><au>Currall, B.B.</au><au>Grills, G.S.</au><au>Mason, C.E.</au><au>Solo-Gabriele, H.M.</au><au>Erickson, D.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a field deployable, high-throughput RT-LAMP device as an early warning system for COVID-19 through SARS-CoV-2 measurements in wastewater</atitle><jtitle>The Science of the total environment</jtitle><addtitle>Sci Total Environ</addtitle><date>2024-09-20</date><risdate>2024</risdate><volume>944</volume><spage>173744</spage><pages>173744-</pages><artnum>173744</artnum><issn>0048-9697</issn><issn>1879-1026</issn><eissn>1879-1026</eissn><abstract>Quantification of SARS-CoV-2 RNA copies in wastewater can be used to estimate COVID-19 prevalence in communities. While such results are important for mitigating disease spread, SARS-CoV-2 measurements require sophisticated equipment and trained personnel, for which a centralized laboratory is necessary. This significantly impacts the time to result, defeating its purpose as an early warning detection tool. The objective of this study was to evaluate a field portable device (called MINI) for detecting SARS-CoV-2 viral loads in wastewater using real-time reverse transcriptase loop-mediated isothermal amplification (real-time RT-LAMP). The device was tested using wastewater samples collected from buildings (with 430 to 1430 inhabitants) that had known COVID-19-positive cases. Results show comparable performance of RT-LAMP against reverse transcriptase polymerase chain reaction (RT-qPCR) when detecting SARS-CoV-2 copies in wastewater. Both RT-LAMP and RT-qPCR detected SARS-CoV-2 in wastewater from buildings with at least three positive individuals within a 6-day time frame prior to diagnosis. The large 96-well throughput provided by MINI provided scalability to multi-building detection. The portability of the MINI device enabled decentralized on-site detection, significantly reducing the time to result. The overall findings support the use of RT-LAMP within the MINI configuration as an early detection system for COVID-19 infection using wastewater collected at the building scale.
[Display omitted]
•Decentralized testing of SARS-CoV-2 in wastewater•Wastewater as an early warning system for COVID-19•Wastewater-based surveillance as a tool for the allocation of resources•Real-Time RT-LAMP as an alternative approach to RT-qPCR•Rapid decentralized detection of wastewater</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>38844223</pmid><doi>10.1016/j.scitotenv.2024.173744</doi></addata></record> |
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| ispartof | The Science of the total environment, 2024-09, Vol.944, p.173744, Article 173744 |
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| subjects | COVID-19 - diagnosis COVID-19 infection environment human resources Humans Molecular Diagnostic Techniques - instrumentation Molecular Diagnostic Techniques - methods Nucleic Acid Amplification Techniques - instrumentation Nucleic Acid Amplification Techniques - methods portable equipment reverse transcriptase polymerase chain reaction reverse transcription loop-mediated isothermal amplification RNA RNA, Viral - analysis RT-LAMP RT-qPCR SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Viral Load Wastewater Wastewater - virology Wastewater-based surveillance |
| title | Evaluation of a field deployable, high-throughput RT-LAMP device as an early warning system for COVID-19 through SARS-CoV-2 measurements in wastewater |
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