A UPLC‐MS/MS method for quantification of metabolites in the ethylene biosynthesis pathway and its biological validation in Arabidopsis

Summary The plant hormone ethylene is of vital importance in the regulation of plant development and stress responses. Recent studies revealed that 1‐aminocyclopropane‐1‐carboxylic acid (ACC) plays a role beyond its function as an ethylene precursor. However, the absence of reliable methods to quant...

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Veröffentlicht in:	The New phytologist 2024-08, Vol.243 (3), p.1262-1275
Hauptverfasser:	Cao, Da, Depaepe, Thomas, Sanchez‐Muñoz, Raul, Janssens, Hilde, Lemière, Filip, Willems, Tim, Winne, Johan, Prinsen, Els, Van Der Straeten, Dominique
Format:	Artikel
Sprache:	eng
Schlagworte:	1-aminocyclopropane-1-carboxylic acid Amino Acids, Cyclic - metabolism Analytical chemistry Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Biosynthesis Biosynthetic Pathways Carboxylic acids Chromatography Chromatography, High Pressure Liquid Conjugates Ethene Ethylene ethylene biosynthesis ethylene production Ethylenes - biosynthesis Ethylenes - metabolism Genetic suppression glutamyl‐ACC Hormones jasmonyl‐ACC Liquid chromatography Liquid Chromatography-Mass Spectrometry malonyl‐ACC Mass spectrometry Mass spectroscopy Metabolism Metabolites method validation Mutation - genetics plant development Plant growth substances Plant hormones Reproducibility of Results Stress, Physiological Submergence synthetic chemistry of standards tandem mass spectrometry Tandem Mass Spectrometry - methods ultra-performance liquid chromatography UPLC‐MS/MS quantification method Wounding
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