Structural Characterization and Immunomodulatory Activity of a Mannose-Rich Polysaccharide Isolated from Bifidobacterium breve H4–2

In this study, a novel homogeneous mannose-rich polysaccharide named EPS-1 from the fermentation broth of Bifidobacterium breve H4–2 was isolated and purified by anion exchange column chromatography and gel column chromatography. The primary structure of EPS-1 was analyzed by high-performance liquid...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2023-12, Vol.71 (49), p.19791-19803
Hauptverfasser:	Niu, Meng-Meng, Guo, Huan-Xin, Shang, Jia-Cui, Meng, Xiang-Chen
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals anion exchange Bifidobacterium breve Bifidobacterium breve - metabolism culture media food chemistry Fourier transform infrared spectroscopy Functional Structure/Activity Relationships gas chromatography-mass spectrometry gels glucose high performance liquid chromatography immunomodulators interleukin-6 macrophages Macrophages - metabolism mannose Mannose - metabolism Mice Molecular Weight NF-kappa B - genetics NF-kappa B - metabolism nuclear magnetic resonance spectroscopy phagocytosis phosphorylation polysaccharides Polysaccharides - chemistry RAW 264.7 Cells secretion thermal stability Toll-like receptor 4 viability
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