Gene expression of iron transporters, markers of vascularization and structural integrity in placenta of mothers with and without anemia
Objective To compare the mRNA expression of placental iron transporters (TfR-1 and FPN), markers of placental vascularization (VEGF and sFLT1) and marker of structural integrity (LMN-A) in term women with and without iron deficiency anemia. Materials and methods A total of 30 pregnant women were enr...
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| Veröffentlicht in: | Molecular biology reports 2024-12, Vol.51 (1), p.652-652, Article 652 |
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| creator | Arora, Madhav Mehndiratta, Mohit Almeida, Edelbert Anthonio Kotru, Mrinalini Gupta, Bindiya |
| description | Objective
To compare the mRNA expression of placental iron transporters (TfR-1 and FPN), markers of placental vascularization (VEGF and sFLT1) and marker of structural integrity (LMN-A) in term women with and without iron deficiency anemia.
Materials and methods
A total of 30 pregnant women were enrolled; 15 cases of iron deficiency anemia (Hb 7-10.9 gm/dL) and 15 gestational age matched healthy controls (Hb ≥ 11 gm/dL). Peripheral venous blood was collected for assessment of hemoglobin levels and serum iron profile. Placental tissue was used for assessing the mRNA expression of TfR-1, FPN, VEGF, sFLT-1 and LMN-A via real time PCR.
Results
Placental expression of TfR-1, VEGF and LMN-A was increased in pregnant women with anemia compared to healthy pregnant controls. Placental expression of sFLT-1 was decreased in pregnant women with anemia compared to healthy pregnant controls. There was no change in the placental expression of FPN.
Conclusion
The increased expression of TfR-1, VEGF and LMN-A in cases of iron deficiency anemia are most likely to be compensatory in nature to help maintain adequate fetal iron delivery.
What does this study adds to the clinical work
: Compensatory changes in the placenta aimed at buffering transport of iron to the fetus are seen in pregnant women with anemia compared to healthy pregnant controls. |
| doi_str_mv | 10.1007/s11033-024-09609-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153647509</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3053630274</sourcerecordid><originalsourceid>FETCH-LOGICAL-c359t-60ca2d051947807d6555d4fc227693eff43b4f673e64058ea632ab43d6653d13</originalsourceid><addsrcrecordid>eNqFkc1O3DAUha2qqExpX6CLKlI3LBp6neufeIlQC0hI3bC3PIkDpkkcbAfKPAGPXWcGisSiXd0j3e8c_xxCPlE4ogDyW6QUEEuoWAlKgCo3b8iKcoklU7J-S1aAQEtWc7pP3sd4AwCMSv6O7GMtkUlVrcjjqR1tYX9Pwcbo_Fj4rnAhzxTMGCcfkg3xazGY8CuLZXtnYjP3JriNSYvBjG0RU5ibNAfTF25M9iq49JBVMfWmsWMyi2_w6XqJuHfpemtahJ9T1nZw5gPZ60wf7ceneUAuf3y_PDkrL36enp8cX5QNcpVKAY2pWuBUMVmDbAXnvGVdU1VSKLRdx3DNOiHRCga8tkZgZdYMWyE4thQPyOEudgr-drYx6cHFxvZ9voWfo0bKUTDJQf0fBY5KKFGzjH55hd74OYz5HVtKIFRyoaod1QQfY7CdnoLLP_ugKeilUb1rVOdG9bZRvcmmz0_R83qw7V_Lc4UZwB0Q82q8suHl7H_E_gGAnq3g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3053630274</pqid></control><display><type>article</type><title>Gene expression of iron transporters, markers of vascularization and structural integrity in placenta of mothers with and without anemia</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Arora, Madhav ; Mehndiratta, Mohit ; Almeida, Edelbert Anthonio ; Kotru, Mrinalini ; Gupta, Bindiya</creator><creatorcontrib>Arora, Madhav ; Mehndiratta, Mohit ; Almeida, Edelbert Anthonio ; Kotru, Mrinalini ; Gupta, Bindiya</creatorcontrib><description>Objective
To compare the mRNA expression of placental iron transporters (TfR-1 and FPN), markers of placental vascularization (VEGF and sFLT1) and marker of structural integrity (LMN-A) in term women with and without iron deficiency anemia.
Materials and methods
A total of 30 pregnant women were enrolled; 15 cases of iron deficiency anemia (Hb 7-10.9 gm/dL) and 15 gestational age matched healthy controls (Hb ≥ 11 gm/dL). Peripheral venous blood was collected for assessment of hemoglobin levels and serum iron profile. Placental tissue was used for assessing the mRNA expression of TfR-1, FPN, VEGF, sFLT-1 and LMN-A via real time PCR.
Results
Placental expression of TfR-1, VEGF and LMN-A was increased in pregnant women with anemia compared to healthy pregnant controls. Placental expression of sFLT-1 was decreased in pregnant women with anemia compared to healthy pregnant controls. There was no change in the placental expression of FPN.
Conclusion
The increased expression of TfR-1, VEGF and LMN-A in cases of iron deficiency anemia are most likely to be compensatory in nature to help maintain adequate fetal iron delivery.
What does this study adds to the clinical work
: Compensatory changes in the placenta aimed at buffering transport of iron to the fetus are seen in pregnant women with anemia compared to healthy pregnant controls.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-024-09609-z</identifier><identifier>PMID: 38734792</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adult ; Anemia ; Anemia, Iron-Deficiency - genetics ; Anemia, Iron-Deficiency - metabolism ; Animal Anatomy ; Animal Biochemistry ; Antigens, CD - genetics ; Antigens, CD - metabolism ; Biomarkers - blood ; Biomarkers - metabolism ; Biomedical and Life Sciences ; blood serum ; Brief Report ; Case-Control Studies ; Cation Transport Proteins - genetics ; Cation Transport Proteins - metabolism ; Deficiency diseases ; Female ; fetus ; Fetuses ; Gene expression ; Gene Expression - genetics ; Gestational age ; Hemoglobin ; Histology ; Humans ; Iron ; Iron - metabolism ; Iron deficiency ; iron deficiency anemia ; Life Sciences ; Morphology ; Placenta ; Placenta - metabolism ; Pregnancy ; quantitative polymerase chain reaction ; Receptors, Transferrin - genetics ; Receptors, Transferrin - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - genetics ; Vascular Endothelial Growth Factor A - metabolism ; Vascular Endothelial Growth Factor Receptor-1 - genetics ; Vascular Endothelial Growth Factor Receptor-1 - metabolism ; Vascularization</subject><ispartof>Molecular biology reports, 2024-12, Vol.51 (1), p.652-652, Article 652</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-60ca2d051947807d6555d4fc227693eff43b4f673e64058ea632ab43d6653d13</cites><orcidid>0009-0006-6010-0355 ; 0000-0002-0822-6544 ; 0000-0002-2555-9607 ; 0000-0001-8301-7964</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-024-09609-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-024-09609-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38734792$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arora, Madhav</creatorcontrib><creatorcontrib>Mehndiratta, Mohit</creatorcontrib><creatorcontrib>Almeida, Edelbert Anthonio</creatorcontrib><creatorcontrib>Kotru, Mrinalini</creatorcontrib><creatorcontrib>Gupta, Bindiya</creatorcontrib><title>Gene expression of iron transporters, markers of vascularization and structural integrity in placenta of mothers with and without anemia</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Objective
To compare the mRNA expression of placental iron transporters (TfR-1 and FPN), markers of placental vascularization (VEGF and sFLT1) and marker of structural integrity (LMN-A) in term women with and without iron deficiency anemia.
Materials and methods
A total of 30 pregnant women were enrolled; 15 cases of iron deficiency anemia (Hb 7-10.9 gm/dL) and 15 gestational age matched healthy controls (Hb ≥ 11 gm/dL). Peripheral venous blood was collected for assessment of hemoglobin levels and serum iron profile. Placental tissue was used for assessing the mRNA expression of TfR-1, FPN, VEGF, sFLT-1 and LMN-A via real time PCR.
Results
Placental expression of TfR-1, VEGF and LMN-A was increased in pregnant women with anemia compared to healthy pregnant controls. Placental expression of sFLT-1 was decreased in pregnant women with anemia compared to healthy pregnant controls. There was no change in the placental expression of FPN.
Conclusion
The increased expression of TfR-1, VEGF and LMN-A in cases of iron deficiency anemia are most likely to be compensatory in nature to help maintain adequate fetal iron delivery.
What does this study adds to the clinical work
: Compensatory changes in the placenta aimed at buffering transport of iron to the fetus are seen in pregnant women with anemia compared to healthy pregnant controls.</description><subject>Adult</subject><subject>Anemia</subject><subject>Anemia, Iron-Deficiency - genetics</subject><subject>Anemia, Iron-Deficiency - metabolism</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Antigens, CD - genetics</subject><subject>Antigens, CD - metabolism</subject><subject>Biomarkers - blood</subject><subject>Biomarkers - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>blood serum</subject><subject>Brief Report</subject><subject>Case-Control Studies</subject><subject>Cation Transport Proteins - genetics</subject><subject>Cation Transport Proteins - metabolism</subject><subject>Deficiency diseases</subject><subject>Female</subject><subject>fetus</subject><subject>Fetuses</subject><subject>Gene expression</subject><subject>Gene Expression - genetics</subject><subject>Gestational age</subject><subject>Hemoglobin</subject><subject>Histology</subject><subject>Humans</subject><subject>Iron</subject><subject>Iron - metabolism</subject><subject>Iron deficiency</subject><subject>iron deficiency anemia</subject><subject>Life Sciences</subject><subject>Morphology</subject><subject>Placenta</subject><subject>Placenta - metabolism</subject><subject>Pregnancy</subject><subject>quantitative polymerase chain reaction</subject><subject>Receptors, Transferrin - genetics</subject><subject>Receptors, Transferrin - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Vascular Endothelial Growth Factor Receptor-1 - genetics</subject><subject>Vascular Endothelial Growth Factor Receptor-1 - metabolism</subject><subject>Vascularization</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1O3DAUha2qqExpX6CLKlI3LBp6neufeIlQC0hI3bC3PIkDpkkcbAfKPAGPXWcGisSiXd0j3e8c_xxCPlE4ogDyW6QUEEuoWAlKgCo3b8iKcoklU7J-S1aAQEtWc7pP3sd4AwCMSv6O7GMtkUlVrcjjqR1tYX9Pwcbo_Fj4rnAhzxTMGCcfkg3xazGY8CuLZXtnYjP3JriNSYvBjG0RU5ibNAfTF25M9iq49JBVMfWmsWMyi2_w6XqJuHfpemtahJ9T1nZw5gPZ60wf7ceneUAuf3y_PDkrL36enp8cX5QNcpVKAY2pWuBUMVmDbAXnvGVdU1VSKLRdx3DNOiHRCga8tkZgZdYMWyE4thQPyOEudgr-drYx6cHFxvZ9voWfo0bKUTDJQf0fBY5KKFGzjH55hd74OYz5HVtKIFRyoaod1QQfY7CdnoLLP_ugKeilUb1rVOdG9bZRvcmmz0_R83qw7V_Lc4UZwB0Q82q8suHl7H_E_gGAnq3g</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Arora, Madhav</creator><creator>Mehndiratta, Mohit</creator><creator>Almeida, Edelbert Anthonio</creator><creator>Kotru, Mrinalini</creator><creator>Gupta, Bindiya</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0009-0006-6010-0355</orcidid><orcidid>https://orcid.org/0000-0002-0822-6544</orcidid><orcidid>https://orcid.org/0000-0002-2555-9607</orcidid><orcidid>https://orcid.org/0000-0001-8301-7964</orcidid></search><sort><creationdate>20241201</creationdate><title>Gene expression of iron transporters, markers of vascularization and structural integrity in placenta of mothers with and without anemia</title><author>Arora, Madhav ; Mehndiratta, Mohit ; Almeida, Edelbert Anthonio ; Kotru, Mrinalini ; Gupta, Bindiya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-60ca2d051947807d6555d4fc227693eff43b4f673e64058ea632ab43d6653d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adult</topic><topic>Anemia</topic><topic>Anemia, Iron-Deficiency - genetics</topic><topic>Anemia, Iron-Deficiency - metabolism</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Antigens, CD - genetics</topic><topic>Antigens, CD - metabolism</topic><topic>Biomarkers - blood</topic><topic>Biomarkers - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>blood serum</topic><topic>Brief Report</topic><topic>Case-Control Studies</topic><topic>Cation Transport Proteins - genetics</topic><topic>Cation Transport Proteins - metabolism</topic><topic>Deficiency diseases</topic><topic>Female</topic><topic>fetus</topic><topic>Fetuses</topic><topic>Gene expression</topic><topic>Gene Expression - genetics</topic><topic>Gestational age</topic><topic>Hemoglobin</topic><topic>Histology</topic><topic>Humans</topic><topic>Iron</topic><topic>Iron - metabolism</topic><topic>Iron deficiency</topic><topic>iron deficiency anemia</topic><topic>Life Sciences</topic><topic>Morphology</topic><topic>Placenta</topic><topic>Placenta - metabolism</topic><topic>Pregnancy</topic><topic>quantitative polymerase chain reaction</topic><topic>Receptors, Transferrin - genetics</topic><topic>Receptors, Transferrin - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>Vascular Endothelial Growth Factor Receptor-1 - genetics</topic><topic>Vascular Endothelial Growth Factor Receptor-1 - metabolism</topic><topic>Vascularization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arora, Madhav</creatorcontrib><creatorcontrib>Mehndiratta, Mohit</creatorcontrib><creatorcontrib>Almeida, Edelbert Anthonio</creatorcontrib><creatorcontrib>Kotru, Mrinalini</creatorcontrib><creatorcontrib>Gupta, Bindiya</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arora, Madhav</au><au>Mehndiratta, Mohit</au><au>Almeida, Edelbert Anthonio</au><au>Kotru, Mrinalini</au><au>Gupta, Bindiya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression of iron transporters, markers of vascularization and structural integrity in placenta of mothers with and without anemia</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>51</volume><issue>1</issue><spage>652</spage><epage>652</epage><pages>652-652</pages><artnum>652</artnum><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Objective
To compare the mRNA expression of placental iron transporters (TfR-1 and FPN), markers of placental vascularization (VEGF and sFLT1) and marker of structural integrity (LMN-A) in term women with and without iron deficiency anemia.
Materials and methods
A total of 30 pregnant women were enrolled; 15 cases of iron deficiency anemia (Hb 7-10.9 gm/dL) and 15 gestational age matched healthy controls (Hb ≥ 11 gm/dL). Peripheral venous blood was collected for assessment of hemoglobin levels and serum iron profile. Placental tissue was used for assessing the mRNA expression of TfR-1, FPN, VEGF, sFLT-1 and LMN-A via real time PCR.
Results
Placental expression of TfR-1, VEGF and LMN-A was increased in pregnant women with anemia compared to healthy pregnant controls. Placental expression of sFLT-1 was decreased in pregnant women with anemia compared to healthy pregnant controls. There was no change in the placental expression of FPN.
Conclusion
The increased expression of TfR-1, VEGF and LMN-A in cases of iron deficiency anemia are most likely to be compensatory in nature to help maintain adequate fetal iron delivery.
What does this study adds to the clinical work
: Compensatory changes in the placenta aimed at buffering transport of iron to the fetus are seen in pregnant women with anemia compared to healthy pregnant controls.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>38734792</pmid><doi>10.1007/s11033-024-09609-z</doi><tpages>1</tpages><orcidid>https://orcid.org/0009-0006-6010-0355</orcidid><orcidid>https://orcid.org/0000-0002-0822-6544</orcidid><orcidid>https://orcid.org/0000-0002-2555-9607</orcidid><orcidid>https://orcid.org/0000-0001-8301-7964</orcidid></addata></record> |
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| subjects | Adult Anemia Anemia, Iron-Deficiency - genetics Anemia, Iron-Deficiency - metabolism Animal Anatomy Animal Biochemistry Antigens, CD - genetics Antigens, CD - metabolism Biomarkers - blood Biomarkers - metabolism Biomedical and Life Sciences blood serum Brief Report Case-Control Studies Cation Transport Proteins - genetics Cation Transport Proteins - metabolism Deficiency diseases Female fetus Fetuses Gene expression Gene Expression - genetics Gestational age Hemoglobin Histology Humans Iron Iron - metabolism Iron deficiency iron deficiency anemia Life Sciences Morphology Placenta Placenta - metabolism Pregnancy quantitative polymerase chain reaction Receptors, Transferrin - genetics Receptors, Transferrin - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Vascular endothelial growth factor Vascular Endothelial Growth Factor A - genetics Vascular Endothelial Growth Factor A - metabolism Vascular Endothelial Growth Factor Receptor-1 - genetics Vascular Endothelial Growth Factor Receptor-1 - metabolism Vascularization |
| title | Gene expression of iron transporters, markers of vascularization and structural integrity in placenta of mothers with and without anemia |
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