Profiles of interferon-stimulated genes in multiple tissues and circulating pregnancy-associated glycoproteins and their association with pregnancy loss in dairy cows
Pregnancy loss (PL) in lactating dairy cows disrupts reproductive and productive efficiency. We evaluated the expression of interferon-stimulated genes (ISG) in blood leukocytes, vaginal and cervical epithelial cells, luteolysis-related genes, progesterone, and pregnancy-associated glycoprotein (PAG...
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| Veröffentlicht in: | Biology of reproduction 2024-03, Vol.110 (3), p.558-568 |
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| creator | Domingues, Rafael R. Andrade, Joao Paulo N. Cunha, Thiago O. Madureira, Guilherme Hoppman, August S. Teixeira, Natalia N. Monteiro, Pedro L.J. Gomez-Leon, Victor H. Martins, Joao Paulo N. Wiltbank, Milo C. |
| description | Pregnancy loss (PL) in lactating dairy cows disrupts reproductive and productive efficiency. We evaluated the expression of interferon-stimulated genes (ISG) in blood leukocytes, vaginal and cervical epithelial cells, luteolysis-related genes, progesterone, and pregnancy-associated glycoprotein (PAG) profiles in lactating dairy cows (n = 86) to gain insight about PL. Expression of ISG on d17, d19, and d21 was greater in cows that maintained the pregnancy (P33) compared to nonpregnant with no PL (NP). Greater ISG differences between groups were observed in the cervix (96.7-fold) than vagina (31.0-fold), and least in blood leukocytes (5.6-fold). Based on individual profiles of ISG and PAG, PL was determined to occur either before (∼13%) or after (∼25%) d22. For cows with PL before d22, ISG expression was similar on d17 but by d21 was lower and OXTR was greater than P33 cows and similar to NP; timing of luteolysis was similar compared to NP cows suggesting embryonic failure to promote luteal maintenance and to attach to the endometrium (no increase in PAG). For cows with PL after d22, ISG expression was similar to P33 cows on d17, d19, and d21 and luteolysis, when it occurred, was later than NP cows; delayed increase in PAG suggested later or inadequate embryonic attachment. In conclusion, PL before d22 occurred due to embryonic demise/failure to signal for luteal maintenance, as reflected in reduced ISG expression by d21. Alternatively, embryos with PL between d22 and 33 adequately signaled for luteal maintenance (ISG) but had delayed/inadequate embryonic attachment and/or inappropriate luteolysis causing PL. Summary Sentence Evaluation of interferon-stimulated genes in multiple tissues showed that the cervix had the greatest expression and revealed that ∼13% of pregnancy loss occurred between d17 and 22, whereas ∼25% of pregnancy loss occurred between d22 and 33. Graphical Abstract |
| doi_str_mv | 10.1093/biolre/ioad164 |
| format | Article |
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We evaluated the expression of interferon-stimulated genes (ISG) in blood leukocytes, vaginal and cervical epithelial cells, luteolysis-related genes, progesterone, and pregnancy-associated glycoprotein (PAG) profiles in lactating dairy cows (n = 86) to gain insight about PL. Expression of ISG on d17, d19, and d21 was greater in cows that maintained the pregnancy (P33) compared to nonpregnant with no PL (NP). Greater ISG differences between groups were observed in the cervix (96.7-fold) than vagina (31.0-fold), and least in blood leukocytes (5.6-fold). Based on individual profiles of ISG and PAG, PL was determined to occur either before (∼13%) or after (∼25%) d22. For cows with PL before d22, ISG expression was similar on d17 but by d21 was lower and OXTR was greater than P33 cows and similar to NP; timing of luteolysis was similar compared to NP cows suggesting embryonic failure to promote luteal maintenance and to attach to the endometrium (no increase in PAG). For cows with PL after d22, ISG expression was similar to P33 cows on d17, d19, and d21 and luteolysis, when it occurred, was later than NP cows; delayed increase in PAG suggested later or inadequate embryonic attachment. In conclusion, PL before d22 occurred due to embryonic demise/failure to signal for luteal maintenance, as reflected in reduced ISG expression by d21. Alternatively, embryos with PL between d22 and 33 adequately signaled for luteal maintenance (ISG) but had delayed/inadequate embryonic attachment and/or inappropriate luteolysis causing PL. Summary Sentence Evaluation of interferon-stimulated genes in multiple tissues showed that the cervix had the greatest expression and revealed that ∼13% of pregnancy loss occurred between d17 and 22, whereas ∼25% of pregnancy loss occurred between d22 and 33. Graphical Abstract</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1093/biolre/ioad164</identifier><identifier>PMID: 38079518</identifier><language>eng</language><publisher>United States: Society for the Study of Reproduction</publisher><subject>Animal lactation ; blood ; Cervix ; Dairy cattle ; early embryonic loss ; embryo mortality ; Embryos ; Endometrium ; Epithelial cells ; epithelium ; Glycoproteins ; Interferon ; ISG15 ; Leukocytes ; luteolysis ; PAG ; Pregnancy ; Progesterone ; PSPB ; RESEARCH ARTICLE ; Vagina</subject><ispartof>Biology of reproduction, 2024-03, Vol.110 (3), p.558-568</ispartof><rights>The Author(s) 2023. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2023</rights><rights>The Author(s) 2023. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><rights>The Author(s) 2023. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b427t-b9fcabd8fd644904c5b186b62e3749291caa23948e40ccc241c60ddefef643683</citedby><cites>FETCH-LOGICAL-b427t-b9fcabd8fd644904c5b186b62e3749291caa23948e40ccc241c60ddefef643683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38079518$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Domingues, Rafael R.</creatorcontrib><creatorcontrib>Andrade, Joao Paulo N.</creatorcontrib><creatorcontrib>Cunha, Thiago O.</creatorcontrib><creatorcontrib>Madureira, Guilherme</creatorcontrib><creatorcontrib>Hoppman, August S.</creatorcontrib><creatorcontrib>Teixeira, Natalia N.</creatorcontrib><creatorcontrib>Monteiro, Pedro L.J.</creatorcontrib><creatorcontrib>Gomez-Leon, Victor H.</creatorcontrib><creatorcontrib>Martins, Joao Paulo N.</creatorcontrib><creatorcontrib>Wiltbank, Milo C.</creatorcontrib><title>Profiles of interferon-stimulated genes in multiple tissues and circulating pregnancy-associated glycoproteins and their association with pregnancy loss in dairy cows</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Pregnancy loss (PL) in lactating dairy cows disrupts reproductive and productive efficiency. We evaluated the expression of interferon-stimulated genes (ISG) in blood leukocytes, vaginal and cervical epithelial cells, luteolysis-related genes, progesterone, and pregnancy-associated glycoprotein (PAG) profiles in lactating dairy cows (n = 86) to gain insight about PL. Expression of ISG on d17, d19, and d21 was greater in cows that maintained the pregnancy (P33) compared to nonpregnant with no PL (NP). Greater ISG differences between groups were observed in the cervix (96.7-fold) than vagina (31.0-fold), and least in blood leukocytes (5.6-fold). Based on individual profiles of ISG and PAG, PL was determined to occur either before (∼13%) or after (∼25%) d22. For cows with PL before d22, ISG expression was similar on d17 but by d21 was lower and OXTR was greater than P33 cows and similar to NP; timing of luteolysis was similar compared to NP cows suggesting embryonic failure to promote luteal maintenance and to attach to the endometrium (no increase in PAG). For cows with PL after d22, ISG expression was similar to P33 cows on d17, d19, and d21 and luteolysis, when it occurred, was later than NP cows; delayed increase in PAG suggested later or inadequate embryonic attachment. In conclusion, PL before d22 occurred due to embryonic demise/failure to signal for luteal maintenance, as reflected in reduced ISG expression by d21. Alternatively, embryos with PL between d22 and 33 adequately signaled for luteal maintenance (ISG) but had delayed/inadequate embryonic attachment and/or inappropriate luteolysis causing PL. Summary Sentence Evaluation of interferon-stimulated genes in multiple tissues showed that the cervix had the greatest expression and revealed that ∼13% of pregnancy loss occurred between d17 and 22, whereas ∼25% of pregnancy loss occurred between d22 and 33. Graphical Abstract</description><subject>Animal lactation</subject><subject>blood</subject><subject>Cervix</subject><subject>Dairy cattle</subject><subject>early embryonic loss</subject><subject>embryo mortality</subject><subject>Embryos</subject><subject>Endometrium</subject><subject>Epithelial cells</subject><subject>epithelium</subject><subject>Glycoproteins</subject><subject>Interferon</subject><subject>ISG15</subject><subject>Leukocytes</subject><subject>luteolysis</subject><subject>PAG</subject><subject>Pregnancy</subject><subject>Progesterone</subject><subject>PSPB</subject><subject>RESEARCH ARTICLE</subject><subject>Vagina</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkU9rFTEUxYMo9lndupSAGwtOm3-TmSylWBUKutD1kEnuvKbkJWOSobwv5Oc0z3lVcPNWgZPfOfdeDkKvKbmkRPGr0UWf4MpFbakUT9CGtkw1HZP9U7QhhMiGc8nP0Iuc7wmhgjP-HJ3xnnSqpf0G_fqW4uQ8ZBwn7EKBNEGKocnF7RavC1i8hVC_XcBVKG72gIvLeamaDhYbl8wBdGGL5wTboIPZNzrnaNxq93sT5xQLuLBayh24hB8RFwN-cOXunxv7mP8MtNqlPTbxIb9EzybtM7w6vufox83H79efm9uvn75cf7htRsG60oxqMnq0_WSlEIoI0460l6NkwDuhmKJGa8aV6EEQYwwT1EhiLUwwScFlz8_RuzW3LvyznliGncsGvNcB4pIHTlsuhRR9dxJlijDFW85IRd_-h97HJYV6SA1kPSFc8MPsy5Uyqd6fYBrm5HY67QdKhkPZw1r2cCy7Gt4cY5dxB_Yv_thuBS5WIC7z6bD3K1v1GOAU_htLC8m3</recordid><startdate>20240313</startdate><enddate>20240313</enddate><creator>Domingues, Rafael R.</creator><creator>Andrade, Joao Paulo N.</creator><creator>Cunha, Thiago O.</creator><creator>Madureira, Guilherme</creator><creator>Hoppman, August S.</creator><creator>Teixeira, Natalia N.</creator><creator>Monteiro, Pedro L.J.</creator><creator>Gomez-Leon, Victor H.</creator><creator>Martins, Joao Paulo N.</creator><creator>Wiltbank, Milo C.</creator><general>Society for the Study of Reproduction</general><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240313</creationdate><title>Profiles of interferon-stimulated genes in multiple tissues and circulating pregnancy-associated glycoproteins and their association with pregnancy loss in dairy cows</title><author>Domingues, Rafael R. ; Andrade, Joao Paulo N. ; Cunha, Thiago O. ; Madureira, Guilherme ; Hoppman, August S. ; Teixeira, Natalia N. ; Monteiro, Pedro L.J. ; Gomez-Leon, Victor H. ; Martins, Joao Paulo N. ; Wiltbank, Milo C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b427t-b9fcabd8fd644904c5b186b62e3749291caa23948e40ccc241c60ddefef643683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animal lactation</topic><topic>blood</topic><topic>Cervix</topic><topic>Dairy cattle</topic><topic>early embryonic loss</topic><topic>embryo mortality</topic><topic>Embryos</topic><topic>Endometrium</topic><topic>Epithelial cells</topic><topic>epithelium</topic><topic>Glycoproteins</topic><topic>Interferon</topic><topic>ISG15</topic><topic>Leukocytes</topic><topic>luteolysis</topic><topic>PAG</topic><topic>Pregnancy</topic><topic>Progesterone</topic><topic>PSPB</topic><topic>RESEARCH ARTICLE</topic><topic>Vagina</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Domingues, Rafael R.</creatorcontrib><creatorcontrib>Andrade, Joao Paulo N.</creatorcontrib><creatorcontrib>Cunha, Thiago O.</creatorcontrib><creatorcontrib>Madureira, Guilherme</creatorcontrib><creatorcontrib>Hoppman, August S.</creatorcontrib><creatorcontrib>Teixeira, Natalia N.</creatorcontrib><creatorcontrib>Monteiro, Pedro L.J.</creatorcontrib><creatorcontrib>Gomez-Leon, Victor H.</creatorcontrib><creatorcontrib>Martins, Joao Paulo N.</creatorcontrib><creatorcontrib>Wiltbank, Milo C.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Domingues, Rafael R.</au><au>Andrade, Joao Paulo N.</au><au>Cunha, Thiago O.</au><au>Madureira, Guilherme</au><au>Hoppman, August S.</au><au>Teixeira, Natalia N.</au><au>Monteiro, Pedro L.J.</au><au>Gomez-Leon, Victor H.</au><au>Martins, Joao Paulo N.</au><au>Wiltbank, Milo C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Profiles of interferon-stimulated genes in multiple tissues and circulating pregnancy-associated glycoproteins and their association with pregnancy loss in dairy cows</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2024-03-13</date><risdate>2024</risdate><volume>110</volume><issue>3</issue><spage>558</spage><epage>568</epage><pages>558-568</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><abstract>Pregnancy loss (PL) in lactating dairy cows disrupts reproductive and productive efficiency. We evaluated the expression of interferon-stimulated genes (ISG) in blood leukocytes, vaginal and cervical epithelial cells, luteolysis-related genes, progesterone, and pregnancy-associated glycoprotein (PAG) profiles in lactating dairy cows (n = 86) to gain insight about PL. Expression of ISG on d17, d19, and d21 was greater in cows that maintained the pregnancy (P33) compared to nonpregnant with no PL (NP). Greater ISG differences between groups were observed in the cervix (96.7-fold) than vagina (31.0-fold), and least in blood leukocytes (5.6-fold). Based on individual profiles of ISG and PAG, PL was determined to occur either before (∼13%) or after (∼25%) d22. For cows with PL before d22, ISG expression was similar on d17 but by d21 was lower and OXTR was greater than P33 cows and similar to NP; timing of luteolysis was similar compared to NP cows suggesting embryonic failure to promote luteal maintenance and to attach to the endometrium (no increase in PAG). For cows with PL after d22, ISG expression was similar to P33 cows on d17, d19, and d21 and luteolysis, when it occurred, was later than NP cows; delayed increase in PAG suggested later or inadequate embryonic attachment. In conclusion, PL before d22 occurred due to embryonic demise/failure to signal for luteal maintenance, as reflected in reduced ISG expression by d21. Alternatively, embryos with PL between d22 and 33 adequately signaled for luteal maintenance (ISG) but had delayed/inadequate embryonic attachment and/or inappropriate luteolysis causing PL. Summary Sentence Evaluation of interferon-stimulated genes in multiple tissues showed that the cervix had the greatest expression and revealed that ∼13% of pregnancy loss occurred between d17 and 22, whereas ∼25% of pregnancy loss occurred between d22 and 33. Graphical Abstract</abstract><cop>United States</cop><pub>Society for the Study of Reproduction</pub><pmid>38079518</pmid><doi>10.1093/biolre/ioad164</doi><tpages>11</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2024-03, Vol.110 (3), p.558-568 |
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| source | Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animal lactation blood Cervix Dairy cattle early embryonic loss embryo mortality Embryos Endometrium Epithelial cells epithelium Glycoproteins Interferon ISG15 Leukocytes luteolysis PAG Pregnancy Progesterone PSPB RESEARCH ARTICLE Vagina |
| title | Profiles of interferon-stimulated genes in multiple tissues and circulating pregnancy-associated glycoproteins and their association with pregnancy loss in dairy cows |
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