Highly Efficient Biosynthesis of Rebaudioside M8 through Structure-Guided Engineering of Glycosyltransferase UGT94E13

Given the low-calorie, high-sweetness characteristics of steviol glycosides (SGs), developing SGs with improved taste profiles is a key focus. Rebaudioside M8 (Reb M8), a novel non-natural SG derivative obtained through glycosylation at the C-13 position of rebaudioside D (Reb D) using glycosyltrans...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2024-07, Vol.72 (28), p.15823-15831
Hauptverfasser:	Yang, Lifeng, Yang, Mengliang, Deng, Zhiwei, Luo, Zhengshan, Yuan, Zhenbo, Rao, Yijian, Zhang, Yan
Format:	Artikel
Sprache:	eng
Schlagworte:	biosynthesis Biotechnology and Biological Transformations catalytic activity commercialization Diterpenes, Kaurane - chemistry Diterpenes, Kaurane - metabolism enzyme activity food chemistry glucose Glucosyltransferases - chemistry Glucosyltransferases - genetics Glucosyltransferases - metabolism Glycosides Glycosylation Glycosyltransferases - chemistry Glycosyltransferases - genetics Glycosyltransferases - metabolism molecular dynamics Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Protein Engineering Stevia - chemistry Stevia - enzymology Stevia - genetics Stevia - metabolism steviol sucrose synthase Sweetening Agents - chemistry Sweetening Agents - metabolism sweetness taste uridine diphosphate
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