Identifying new sources of resistance to tomato leaf curl New Delhi virus from Indian melon germplasm by designing an improved method of field screening
Tomato leaf curl New Delhi virus (ToLCNDV) is an emerging constraint in muskmelon production in India and other parts of the world. This study aims to identify the new sources of resistance against ToLCNDV from Indian melon germplasm, which has not been evaluated globally. Sixty melon germplasm com...
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| Veröffentlicht in: | Genetic resources and crop evolution 2024-06, Vol.71 (5), p.1911-1933 |
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| container_title | Genetic resources and crop evolution |
| container_volume | 71 |
| creator | Padmanabha, K. Choudhary, Harshawardhan Mishra, G. P. Mandal, Bikash Solanke, A. U. Mishra, D. C. Yadav, R. K. |
| description | Tomato leaf curl New Delhi virus
(ToLCNDV) is an emerging constraint in muskmelon production in India and other parts of the world. This study aims to identify the new sources of resistance against ToLCNDV from Indian melon germplasm, which has not been evaluated globally. Sixty melon germplasm comprising of both cultivated commercial types (vars.
reticulatus
and
inodorus
) from the subspecies
melo
and wild germplasm (vars.
momordica,
conomon
, and
callosus)
from subspecies
agrestis
were screened in the field for two consecutive years under natural epiphytotic condition. The infected plants showed varying degrees of phenotypic symptoms, such as yellow mosaic, stunting of plant growth, and restricted fruiting. The disease response of ToLCNDV in melon genotypes were measured by a robust rating scale, which was developed by providing differential weightage to morphogenic symptoms on foliage, reduction of vine length and fruiting of the plant. The genotype DSM 132 (
C.melo
var.
callosus)
could be identified as highly resistant to ToLCNDV
,
which recorded the minimum disease severity index (DSI) of 0.00, 0.00, followed by DSM 19 (3.50, 4.50) and DSM-11-7 (7.00, 6.11) from
C. melo
var.
momordica
for two consecutive years. The resistance in these genotypes was further confirmed through challenge inoculation with viruliferous whitefly (
Bemisia tabaci
) carrying ToLCNDV in the greenhouse conditions, which showed a minimum vulnerability index in genotype DSM 132 (VI = 2.0) followed by DSM 19 (VI = 6.67) and DSM-11-7 (VI = 11.34). The molecular technique of virus detection through polymerase chain reaction (PCR) specific to ToLCNDV failed to detect the presence of tomato leaf curl New Delhi virus in resistant genotypes DSM 132, DSM 19, and DSM-11-7. Quantitative PCR (qPCR) showed very low viral titer in resistant genotypes DSM 132, DSM 19, and DSM-11-7 compared to susceptible genotypes. This study could identify three Indian melon genotypes with high levels of resistance to ToLCNDV, which will be useful for resistance breeding across the globe. |
| doi_str_mv | 10.1007/s10722-023-01744-z |
| format | Article |
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(ToLCNDV) is an emerging constraint in muskmelon production in India and other parts of the world. This study aims to identify the new sources of resistance against ToLCNDV from Indian melon germplasm, which has not been evaluated globally. Sixty melon germplasm comprising of both cultivated commercial types (vars.
reticulatus
and
inodorus
) from the subspecies
melo
and wild germplasm (vars.
momordica,
conomon
, and
callosus)
from subspecies
agrestis
were screened in the field for two consecutive years under natural epiphytotic condition. The infected plants showed varying degrees of phenotypic symptoms, such as yellow mosaic, stunting of plant growth, and restricted fruiting. The disease response of ToLCNDV in melon genotypes were measured by a robust rating scale, which was developed by providing differential weightage to morphogenic symptoms on foliage, reduction of vine length and fruiting of the plant. The genotype DSM 132 (
C.melo
var.
callosus)
could be identified as highly resistant to ToLCNDV
,
which recorded the minimum disease severity index (DSI) of 0.00, 0.00, followed by DSM 19 (3.50, 4.50) and DSM-11-7 (7.00, 6.11) from
C. melo
var.
momordica
for two consecutive years. The resistance in these genotypes was further confirmed through challenge inoculation with viruliferous whitefly (
Bemisia tabaci
) carrying ToLCNDV in the greenhouse conditions, which showed a minimum vulnerability index in genotype DSM 132 (VI = 2.0) followed by DSM 19 (VI = 6.67) and DSM-11-7 (VI = 11.34). The molecular technique of virus detection through polymerase chain reaction (PCR) specific to ToLCNDV failed to detect the presence of tomato leaf curl New Delhi virus in resistant genotypes DSM 132, DSM 19, and DSM-11-7. Quantitative PCR (qPCR) showed very low viral titer in resistant genotypes DSM 132, DSM 19, and DSM-11-7 compared to susceptible genotypes. This study could identify three Indian melon genotypes with high levels of resistance to ToLCNDV, which will be useful for resistance breeding across the globe.</description><identifier>ISSN: 0925-9864</identifier><identifier>EISSN: 1573-5109</identifier><identifier>DOI: 10.1007/s10722-023-01744-z</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Bemisia tabaci ; Biomedical and Life Sciences ; Cucumis ; Disease resistance ; Foliage ; Fruits ; genotype ; Genotypes ; Germplasm ; greenhouses ; India ; Inoculation ; Leaf-curl ; Leaves ; Life Sciences ; muskmelons ; phenotype ; Plant Genetics and Genomics ; Plant growth ; Plant Physiology ; Plant Sciences ; Plant Systematics/Taxonomy/Biogeography ; Plant viruses ; Polymerase chain reaction ; quantitative polymerase chain reaction ; Research Article ; Signs and symptoms ; Tomato leaf curl New Delhi virus ; Tomatoes ; vines ; viral load ; Viruses</subject><ispartof>Genetic resources and crop evolution, 2024-06, Vol.71 (5), p.1911-1933</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-36e7d2ffdb0da0f61db69f02672dac5b368c0b552714e2eb6664fcc8256941343</citedby><cites>FETCH-LOGICAL-c352t-36e7d2ffdb0da0f61db69f02672dac5b368c0b552714e2eb6664fcc8256941343</cites><orcidid>0000-0002-7760-9979</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10722-023-01744-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10722-023-01744-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids></links><search><creatorcontrib>Padmanabha, K.</creatorcontrib><creatorcontrib>Choudhary, Harshawardhan</creatorcontrib><creatorcontrib>Mishra, G. P.</creatorcontrib><creatorcontrib>Mandal, Bikash</creatorcontrib><creatorcontrib>Solanke, A. U.</creatorcontrib><creatorcontrib>Mishra, D. C.</creatorcontrib><creatorcontrib>Yadav, R. K.</creatorcontrib><title>Identifying new sources of resistance to tomato leaf curl New Delhi virus from Indian melon germplasm by designing an improved method of field screening</title><title>Genetic resources and crop evolution</title><addtitle>Genet Resour Crop Evol</addtitle><description>Tomato leaf curl New Delhi virus
(ToLCNDV) is an emerging constraint in muskmelon production in India and other parts of the world. This study aims to identify the new sources of resistance against ToLCNDV from Indian melon germplasm, which has not been evaluated globally. Sixty melon germplasm comprising of both cultivated commercial types (vars.
reticulatus
and
inodorus
) from the subspecies
melo
and wild germplasm (vars.
momordica,
conomon
, and
callosus)
from subspecies
agrestis
were screened in the field for two consecutive years under natural epiphytotic condition. The infected plants showed varying degrees of phenotypic symptoms, such as yellow mosaic, stunting of plant growth, and restricted fruiting. The disease response of ToLCNDV in melon genotypes were measured by a robust rating scale, which was developed by providing differential weightage to morphogenic symptoms on foliage, reduction of vine length and fruiting of the plant. The genotype DSM 132 (
C.melo
var.
callosus)
could be identified as highly resistant to ToLCNDV
,
which recorded the minimum disease severity index (DSI) of 0.00, 0.00, followed by DSM 19 (3.50, 4.50) and DSM-11-7 (7.00, 6.11) from
C. melo
var.
momordica
for two consecutive years. The resistance in these genotypes was further confirmed through challenge inoculation with viruliferous whitefly (
Bemisia tabaci
) carrying ToLCNDV in the greenhouse conditions, which showed a minimum vulnerability index in genotype DSM 132 (VI = 2.0) followed by DSM 19 (VI = 6.67) and DSM-11-7 (VI = 11.34). The molecular technique of virus detection through polymerase chain reaction (PCR) specific to ToLCNDV failed to detect the presence of tomato leaf curl New Delhi virus in resistant genotypes DSM 132, DSM 19, and DSM-11-7. Quantitative PCR (qPCR) showed very low viral titer in resistant genotypes DSM 132, DSM 19, and DSM-11-7 compared to susceptible genotypes. This study could identify three Indian melon genotypes with high levels of resistance to ToLCNDV, which will be useful for resistance breeding across the globe.</description><subject>Agriculture</subject><subject>Bemisia tabaci</subject><subject>Biomedical and Life Sciences</subject><subject>Cucumis</subject><subject>Disease resistance</subject><subject>Foliage</subject><subject>Fruits</subject><subject>genotype</subject><subject>Genotypes</subject><subject>Germplasm</subject><subject>greenhouses</subject><subject>India</subject><subject>Inoculation</subject><subject>Leaf-curl</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>muskmelons</subject><subject>phenotype</subject><subject>Plant Genetics and Genomics</subject><subject>Plant growth</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Plant Systematics/Taxonomy/Biogeography</subject><subject>Plant viruses</subject><subject>Polymerase chain reaction</subject><subject>quantitative polymerase chain reaction</subject><subject>Research Article</subject><subject>Signs and symptoms</subject><subject>Tomato leaf curl New Delhi virus</subject><subject>Tomatoes</subject><subject>vines</subject><subject>viral load</subject><subject>Viruses</subject><issn>0925-9864</issn><issn>1573-5109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kU1uFDEQhS1EJIbABVhZYsOmif89vUSBwEgR2YS15bbLE0du92B3B01OwnHjZpAisUAqqRb1vVelegi9o-QjJURfVEo0Yx1hvCNUC9E9vkAbKjXvJCX9S7QhPZNdv1XiFXpd6z0hpNdqu0G_dx7yHMMx5j3O8AvXaSkOKp4CLlBjnW12gOep1WhbS2ADdktJ-HujP0O6i_ghlqXiUKYR77KPNuMR0pTxHsp4SLaOeDhi39z2eV3T5nE8lOkBfAPnu8mv20KE5HF1BWCl3qCzYFOFt3_7Ofpx9eX28lt3ffN1d_npunNcsrnjCrRnIfiBeEuCon5QfSBMaeatkwNXW0cGKZmmAhgMSikRnNsyqXpBueDn6MPJtx30c4E6mzFWBynZDNNSDaeSK9ZzvaLv_0Hv27Nyu85wIhRVQjHaKHaiXJlqLRDMocTRlqOhxKxhmVNYpoVl_oRlHpuIn0S1wbn97dn6P6on4nGaOw</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Padmanabha, K.</creator><creator>Choudhary, Harshawardhan</creator><creator>Mishra, G. P.</creator><creator>Mandal, Bikash</creator><creator>Solanke, A. U.</creator><creator>Mishra, D. C.</creator><creator>Yadav, R. K.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-7760-9979</orcidid></search><sort><creationdate>20240601</creationdate><title>Identifying new sources of resistance to tomato leaf curl New Delhi virus from Indian melon germplasm by designing an improved method of field screening</title><author>Padmanabha, K. ; Choudhary, Harshawardhan ; Mishra, G. P. ; Mandal, Bikash ; Solanke, A. U. ; Mishra, D. C. ; Yadav, R. K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-36e7d2ffdb0da0f61db69f02672dac5b368c0b552714e2eb6664fcc8256941343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Agriculture</topic><topic>Bemisia tabaci</topic><topic>Biomedical and Life Sciences</topic><topic>Cucumis</topic><topic>Disease resistance</topic><topic>Foliage</topic><topic>Fruits</topic><topic>genotype</topic><topic>Genotypes</topic><topic>Germplasm</topic><topic>greenhouses</topic><topic>India</topic><topic>Inoculation</topic><topic>Leaf-curl</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>muskmelons</topic><topic>phenotype</topic><topic>Plant Genetics and Genomics</topic><topic>Plant growth</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Plant Systematics/Taxonomy/Biogeography</topic><topic>Plant viruses</topic><topic>Polymerase chain reaction</topic><topic>quantitative polymerase chain reaction</topic><topic>Research Article</topic><topic>Signs and symptoms</topic><topic>Tomato leaf curl New Delhi virus</topic><topic>Tomatoes</topic><topic>vines</topic><topic>viral load</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Padmanabha, K.</creatorcontrib><creatorcontrib>Choudhary, Harshawardhan</creatorcontrib><creatorcontrib>Mishra, G. P.</creatorcontrib><creatorcontrib>Mandal, Bikash</creatorcontrib><creatorcontrib>Solanke, A. U.</creatorcontrib><creatorcontrib>Mishra, D. C.</creatorcontrib><creatorcontrib>Yadav, R. K.</creatorcontrib><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Genetic resources and crop evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Padmanabha, K.</au><au>Choudhary, Harshawardhan</au><au>Mishra, G. P.</au><au>Mandal, Bikash</au><au>Solanke, A. U.</au><au>Mishra, D. C.</au><au>Yadav, R. K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identifying new sources of resistance to tomato leaf curl New Delhi virus from Indian melon germplasm by designing an improved method of field screening</atitle><jtitle>Genetic resources and crop evolution</jtitle><stitle>Genet Resour Crop Evol</stitle><date>2024-06-01</date><risdate>2024</risdate><volume>71</volume><issue>5</issue><spage>1911</spage><epage>1933</epage><pages>1911-1933</pages><issn>0925-9864</issn><eissn>1573-5109</eissn><abstract>Tomato leaf curl New Delhi virus
(ToLCNDV) is an emerging constraint in muskmelon production in India and other parts of the world. This study aims to identify the new sources of resistance against ToLCNDV from Indian melon germplasm, which has not been evaluated globally. Sixty melon germplasm comprising of both cultivated commercial types (vars.
reticulatus
and
inodorus
) from the subspecies
melo
and wild germplasm (vars.
momordica,
conomon
, and
callosus)
from subspecies
agrestis
were screened in the field for two consecutive years under natural epiphytotic condition. The infected plants showed varying degrees of phenotypic symptoms, such as yellow mosaic, stunting of plant growth, and restricted fruiting. The disease response of ToLCNDV in melon genotypes were measured by a robust rating scale, which was developed by providing differential weightage to morphogenic symptoms on foliage, reduction of vine length and fruiting of the plant. The genotype DSM 132 (
C.melo
var.
callosus)
could be identified as highly resistant to ToLCNDV
,
which recorded the minimum disease severity index (DSI) of 0.00, 0.00, followed by DSM 19 (3.50, 4.50) and DSM-11-7 (7.00, 6.11) from
C. melo
var.
momordica
for two consecutive years. The resistance in these genotypes was further confirmed through challenge inoculation with viruliferous whitefly (
Bemisia tabaci
) carrying ToLCNDV in the greenhouse conditions, which showed a minimum vulnerability index in genotype DSM 132 (VI = 2.0) followed by DSM 19 (VI = 6.67) and DSM-11-7 (VI = 11.34). The molecular technique of virus detection through polymerase chain reaction (PCR) specific to ToLCNDV failed to detect the presence of tomato leaf curl New Delhi virus in resistant genotypes DSM 132, DSM 19, and DSM-11-7. Quantitative PCR (qPCR) showed very low viral titer in resistant genotypes DSM 132, DSM 19, and DSM-11-7 compared to susceptible genotypes. This study could identify three Indian melon genotypes with high levels of resistance to ToLCNDV, which will be useful for resistance breeding across the globe.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10722-023-01744-z</doi><tpages>23</tpages><orcidid>https://orcid.org/0000-0002-7760-9979</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0925-9864 |
| ispartof | Genetic resources and crop evolution, 2024-06, Vol.71 (5), p.1911-1933 |
| issn | 0925-9864 1573-5109 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3153629374 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Agriculture Bemisia tabaci Biomedical and Life Sciences Cucumis Disease resistance Foliage Fruits genotype Genotypes Germplasm greenhouses India Inoculation Leaf-curl Leaves Life Sciences muskmelons phenotype Plant Genetics and Genomics Plant growth Plant Physiology Plant Sciences Plant Systematics/Taxonomy/Biogeography Plant viruses Polymerase chain reaction quantitative polymerase chain reaction Research Article Signs and symptoms Tomato leaf curl New Delhi virus Tomatoes vines viral load Viruses |
| title | Identifying new sources of resistance to tomato leaf curl New Delhi virus from Indian melon germplasm by designing an improved method of field screening |
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