Extraction, purification, and investigation of the antibacterial potential of lysozyme isolated from the latex of Calotropis procera
Calotropis procera produces a latex used in traditional medicine because of its components which are found to be biologically active. The latex of C. procera has many benefits such as pain reduction, antimicrobial attitude and other precious attributes. Latex lysozyme (LL) is a particular protein co...
Gespeichert in:
Veröffentlicht in: | European food research & technology 2024-05, Vol.250 (5), p.1401-1416 |
---|---|
Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 1416 |
---|---|
container_issue | 5 |
container_start_page | 1401 |
container_title | European food research & technology |
container_volume | 250 |
creator | Muthu, Sakthivel Gopal, Venkateshbabu AL-Younis, Zena Kadhim Altemimi, Ammar B. Perumal, Palani Lakshmikanthan, Mythileeswari Krishnan, Kathiravan Najm, Mazin A. A. Giuffrè, Angelo Maria Govindan, Lakshmanan |
description | Calotropis procera
produces a latex used in traditional medicine because of its components which are found to be biologically active. The latex of
C. procera
has many benefits such as pain reduction, antimicrobial attitude and other precious attributes. Latex lysozyme (LL) is a particular protein contained in
C. procera
plant having a promising antibacterial property. The process applied for extraction implicated ammonium sulfate precipitation, water extraction, ion exchange and gel filtration column chromatography evidencing 14.4 kDa for LL as a molecular mass. LL after purification showed 397.18 U mg
−1
as specific activity (10.30 purification fold), and with 37.9% as a yield. In addition, the chemical and physical analyses showed that LL had its best realization when the pH was 5.5 at 50 °C. Moreover when metal ions such as Ca
2+
and Mg
2+
were incorporated the relative activity of the enzyme resulted ampliflied. When a purification was conducted with CM-Cellulose and Sephadex G-100 chromatography, LL revealed an antibacterial property versus both
Escherichia coli
(MICs 14 µg ml
−1
) and
Bacillus cereus
(MICs 13 µg ml
−1
). HR-TEM analysis displayed an antimicrobial potential of LL after its purification; that analysis showed the deterioration of the cell wall (external membrane of the cell) of both
E. coli
and
B. cereus
. Therefore, the LL obtained from
C. procera
maintains ability as an antibacterial enzyme applicable in various domains such as biotechnological and pharmaceutical industries.
Graphical abstract |
doi_str_mv | 10.1007/s00217-024-04467-2 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153626678</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3153626678</sourcerecordid><originalsourceid>FETCH-LOGICAL-c347t-f71b9f54c1aa5c04a35d1272359226086b19d5ac31c71eb839d9048a445a52fc3</originalsourceid><addsrcrecordid>eNp9kbtOwzAUhiMEEqXwAkyWWBgI-BonI6rKRarEArPlOHZxlcbBdlDLzIPjNggkBqZz-_5jH_1Zdo7gNYKQ3wQIMeI5xDSHlBY8xwfZBFFS5piU7PAn5_w4OwlhBSGrCkQn2ed8E71U0bruCvSDt8YqOVaya4Dt3nWIdrlvAWdAfNVpEG2dNNpb2YLeRZ0aKUvjdhvcx3atgQ2ulVE3wHi33qt25WbHzGTrone9DaD3TmkvT7MjI9ugz77jNHu5mz_PHvLF0_3j7HaRK0J5zA1HdWUYVUhKpiCVhDUIc0xYhXEBy6JGVcOkIkhxpOuSVE0FaSkpZZJho8g0uxz3pnffhnSYWNugdNvKTrshCIIYKXBR8DKhF3_QlRt8l34ncMUJ46QqYKLwSCnvQvDaiN7btfRbgaDYGSNGY0QyRuyNETiJyCgKCe6W2v-u_kf1BWVtkmo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2973573960</pqid></control><display><type>article</type><title>Extraction, purification, and investigation of the antibacterial potential of lysozyme isolated from the latex of Calotropis procera</title><source>Springer Nature - Complete Springer Journals</source><creator>Muthu, Sakthivel ; Gopal, Venkateshbabu ; AL-Younis, Zena Kadhim ; Altemimi, Ammar B. ; Perumal, Palani ; Lakshmikanthan, Mythileeswari ; Krishnan, Kathiravan ; Najm, Mazin A. A. ; Giuffrè, Angelo Maria ; Govindan, Lakshmanan</creator><creatorcontrib>Muthu, Sakthivel ; Gopal, Venkateshbabu ; AL-Younis, Zena Kadhim ; Altemimi, Ammar B. ; Perumal, Palani ; Lakshmikanthan, Mythileeswari ; Krishnan, Kathiravan ; Najm, Mazin A. A. ; Giuffrè, Angelo Maria ; Govindan, Lakshmanan</creatorcontrib><description>Calotropis procera
produces a latex used in traditional medicine because of its components which are found to be biologically active. The latex of
C. procera
has many benefits such as pain reduction, antimicrobial attitude and other precious attributes. Latex lysozyme (LL) is a particular protein contained in
C. procera
plant having a promising antibacterial property. The process applied for extraction implicated ammonium sulfate precipitation, water extraction, ion exchange and gel filtration column chromatography evidencing 14.4 kDa for LL as a molecular mass. LL after purification showed 397.18 U mg
−1
as specific activity (10.30 purification fold), and with 37.9% as a yield. In addition, the chemical and physical analyses showed that LL had its best realization when the pH was 5.5 at 50 °C. Moreover when metal ions such as Ca
2+
and Mg
2+
were incorporated the relative activity of the enzyme resulted ampliflied. When a purification was conducted with CM-Cellulose and Sephadex G-100 chromatography, LL revealed an antibacterial property versus both
Escherichia coli
(MICs 14 µg ml
−1
) and
Bacillus cereus
(MICs 13 µg ml
−1
). HR-TEM analysis displayed an antimicrobial potential of LL after its purification; that analysis showed the deterioration of the cell wall (external membrane of the cell) of both
E. coli
and
B. cereus
. Therefore, the LL obtained from
C. procera
maintains ability as an antibacterial enzyme applicable in various domains such as biotechnological and pharmaceutical industries.
Graphical abstract</description><identifier>ISSN: 1438-2377</identifier><identifier>EISSN: 1438-2385</identifier><identifier>DOI: 10.1007/s00217-024-04467-2</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Agriculture ; Ammonium ; Ammonium sulfate ; Analytical Chemistry ; antibacterial properties ; Bacillus cereus ; Biological activity ; Biotechnology ; calcium ; Calcium ions ; Calotropis procera ; Cell walls ; Cellulose ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Column chromatography ; E coli ; enzyme activity ; Enzymes ; Escherichia coli ; Food Science ; Forestry ; gel chromatography ; Gel filtration ; Ion exchange ; Latex ; Lysozyme ; Magnesium ; Metal ions ; molecular weight ; Original Paper ; pain ; Pharmaceutical industry ; Purification ; traditional medicine</subject><ispartof>European food research & technology, 2024-05, Vol.250 (5), p.1401-1416</ispartof><rights>The Author(s) 2024</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c347t-f71b9f54c1aa5c04a35d1272359226086b19d5ac31c71eb839d9048a445a52fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00217-024-04467-2$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00217-024-04467-2$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids></links><search><creatorcontrib>Muthu, Sakthivel</creatorcontrib><creatorcontrib>Gopal, Venkateshbabu</creatorcontrib><creatorcontrib>AL-Younis, Zena Kadhim</creatorcontrib><creatorcontrib>Altemimi, Ammar B.</creatorcontrib><creatorcontrib>Perumal, Palani</creatorcontrib><creatorcontrib>Lakshmikanthan, Mythileeswari</creatorcontrib><creatorcontrib>Krishnan, Kathiravan</creatorcontrib><creatorcontrib>Najm, Mazin A. A.</creatorcontrib><creatorcontrib>Giuffrè, Angelo Maria</creatorcontrib><creatorcontrib>Govindan, Lakshmanan</creatorcontrib><title>Extraction, purification, and investigation of the antibacterial potential of lysozyme isolated from the latex of Calotropis procera</title><title>European food research & technology</title><addtitle>Eur Food Res Technol</addtitle><description>Calotropis procera
produces a latex used in traditional medicine because of its components which are found to be biologically active. The latex of
C. procera
has many benefits such as pain reduction, antimicrobial attitude and other precious attributes. Latex lysozyme (LL) is a particular protein contained in
C. procera
plant having a promising antibacterial property. The process applied for extraction implicated ammonium sulfate precipitation, water extraction, ion exchange and gel filtration column chromatography evidencing 14.4 kDa for LL as a molecular mass. LL after purification showed 397.18 U mg
−1
as specific activity (10.30 purification fold), and with 37.9% as a yield. In addition, the chemical and physical analyses showed that LL had its best realization when the pH was 5.5 at 50 °C. Moreover when metal ions such as Ca
2+
and Mg
2+
were incorporated the relative activity of the enzyme resulted ampliflied. When a purification was conducted with CM-Cellulose and Sephadex G-100 chromatography, LL revealed an antibacterial property versus both
Escherichia coli
(MICs 14 µg ml
−1
) and
Bacillus cereus
(MICs 13 µg ml
−1
). HR-TEM analysis displayed an antimicrobial potential of LL after its purification; that analysis showed the deterioration of the cell wall (external membrane of the cell) of both
E. coli
and
B. cereus
. Therefore, the LL obtained from
C. procera
maintains ability as an antibacterial enzyme applicable in various domains such as biotechnological and pharmaceutical industries.
Graphical abstract</description><subject>Agriculture</subject><subject>Ammonium</subject><subject>Ammonium sulfate</subject><subject>Analytical Chemistry</subject><subject>antibacterial properties</subject><subject>Bacillus cereus</subject><subject>Biological activity</subject><subject>Biotechnology</subject><subject>calcium</subject><subject>Calcium ions</subject><subject>Calotropis procera</subject><subject>Cell walls</subject><subject>Cellulose</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Column chromatography</subject><subject>E coli</subject><subject>enzyme activity</subject><subject>Enzymes</subject><subject>Escherichia coli</subject><subject>Food Science</subject><subject>Forestry</subject><subject>gel chromatography</subject><subject>Gel filtration</subject><subject>Ion exchange</subject><subject>Latex</subject><subject>Lysozyme</subject><subject>Magnesium</subject><subject>Metal ions</subject><subject>molecular weight</subject><subject>Original Paper</subject><subject>pain</subject><subject>Pharmaceutical industry</subject><subject>Purification</subject><subject>traditional medicine</subject><issn>1438-2377</issn><issn>1438-2385</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kbtOwzAUhiMEEqXwAkyWWBgI-BonI6rKRarEArPlOHZxlcbBdlDLzIPjNggkBqZz-_5jH_1Zdo7gNYKQ3wQIMeI5xDSHlBY8xwfZBFFS5piU7PAn5_w4OwlhBSGrCkQn2ed8E71U0bruCvSDt8YqOVaya4Dt3nWIdrlvAWdAfNVpEG2dNNpb2YLeRZ0aKUvjdhvcx3atgQ2ulVE3wHi33qt25WbHzGTrone9DaD3TmkvT7MjI9ugz77jNHu5mz_PHvLF0_3j7HaRK0J5zA1HdWUYVUhKpiCVhDUIc0xYhXEBy6JGVcOkIkhxpOuSVE0FaSkpZZJho8g0uxz3pnffhnSYWNugdNvKTrshCIIYKXBR8DKhF3_QlRt8l34ncMUJ46QqYKLwSCnvQvDaiN7btfRbgaDYGSNGY0QyRuyNETiJyCgKCe6W2v-u_kf1BWVtkmo</recordid><startdate>20240501</startdate><enddate>20240501</enddate><creator>Muthu, Sakthivel</creator><creator>Gopal, Venkateshbabu</creator><creator>AL-Younis, Zena Kadhim</creator><creator>Altemimi, Ammar B.</creator><creator>Perumal, Palani</creator><creator>Lakshmikanthan, Mythileeswari</creator><creator>Krishnan, Kathiravan</creator><creator>Najm, Mazin A. A.</creator><creator>Giuffrè, Angelo Maria</creator><creator>Govindan, Lakshmanan</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QR</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240501</creationdate><title>Extraction, purification, and investigation of the antibacterial potential of lysozyme isolated from the latex of Calotropis procera</title><author>Muthu, Sakthivel ; Gopal, Venkateshbabu ; AL-Younis, Zena Kadhim ; Altemimi, Ammar B. ; Perumal, Palani ; Lakshmikanthan, Mythileeswari ; Krishnan, Kathiravan ; Najm, Mazin A. A. ; Giuffrè, Angelo Maria ; Govindan, Lakshmanan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-f71b9f54c1aa5c04a35d1272359226086b19d5ac31c71eb839d9048a445a52fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Agriculture</topic><topic>Ammonium</topic><topic>Ammonium sulfate</topic><topic>Analytical Chemistry</topic><topic>antibacterial properties</topic><topic>Bacillus cereus</topic><topic>Biological activity</topic><topic>Biotechnology</topic><topic>calcium</topic><topic>Calcium ions</topic><topic>Calotropis procera</topic><topic>Cell walls</topic><topic>Cellulose</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography</topic><topic>Column chromatography</topic><topic>E coli</topic><topic>enzyme activity</topic><topic>Enzymes</topic><topic>Escherichia coli</topic><topic>Food Science</topic><topic>Forestry</topic><topic>gel chromatography</topic><topic>Gel filtration</topic><topic>Ion exchange</topic><topic>Latex</topic><topic>Lysozyme</topic><topic>Magnesium</topic><topic>Metal ions</topic><topic>molecular weight</topic><topic>Original Paper</topic><topic>pain</topic><topic>Pharmaceutical industry</topic><topic>Purification</topic><topic>traditional medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muthu, Sakthivel</creatorcontrib><creatorcontrib>Gopal, Venkateshbabu</creatorcontrib><creatorcontrib>AL-Younis, Zena Kadhim</creatorcontrib><creatorcontrib>Altemimi, Ammar B.</creatorcontrib><creatorcontrib>Perumal, Palani</creatorcontrib><creatorcontrib>Lakshmikanthan, Mythileeswari</creatorcontrib><creatorcontrib>Krishnan, Kathiravan</creatorcontrib><creatorcontrib>Najm, Mazin A. A.</creatorcontrib><creatorcontrib>Giuffrè, Angelo Maria</creatorcontrib><creatorcontrib>Govindan, Lakshmanan</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>European food research & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muthu, Sakthivel</au><au>Gopal, Venkateshbabu</au><au>AL-Younis, Zena Kadhim</au><au>Altemimi, Ammar B.</au><au>Perumal, Palani</au><au>Lakshmikanthan, Mythileeswari</au><au>Krishnan, Kathiravan</au><au>Najm, Mazin A. A.</au><au>Giuffrè, Angelo Maria</au><au>Govindan, Lakshmanan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extraction, purification, and investigation of the antibacterial potential of lysozyme isolated from the latex of Calotropis procera</atitle><jtitle>European food research & technology</jtitle><stitle>Eur Food Res Technol</stitle><date>2024-05-01</date><risdate>2024</risdate><volume>250</volume><issue>5</issue><spage>1401</spage><epage>1416</epage><pages>1401-1416</pages><issn>1438-2377</issn><eissn>1438-2385</eissn><abstract>Calotropis procera
produces a latex used in traditional medicine because of its components which are found to be biologically active. The latex of
C. procera
has many benefits such as pain reduction, antimicrobial attitude and other precious attributes. Latex lysozyme (LL) is a particular protein contained in
C. procera
plant having a promising antibacterial property. The process applied for extraction implicated ammonium sulfate precipitation, water extraction, ion exchange and gel filtration column chromatography evidencing 14.4 kDa for LL as a molecular mass. LL after purification showed 397.18 U mg
−1
as specific activity (10.30 purification fold), and with 37.9% as a yield. In addition, the chemical and physical analyses showed that LL had its best realization when the pH was 5.5 at 50 °C. Moreover when metal ions such as Ca
2+
and Mg
2+
were incorporated the relative activity of the enzyme resulted ampliflied. When a purification was conducted with CM-Cellulose and Sephadex G-100 chromatography, LL revealed an antibacterial property versus both
Escherichia coli
(MICs 14 µg ml
−1
) and
Bacillus cereus
(MICs 13 µg ml
−1
). HR-TEM analysis displayed an antimicrobial potential of LL after its purification; that analysis showed the deterioration of the cell wall (external membrane of the cell) of both
E. coli
and
B. cereus
. Therefore, the LL obtained from
C. procera
maintains ability as an antibacterial enzyme applicable in various domains such as biotechnological and pharmaceutical industries.
Graphical abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00217-024-04467-2</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1438-2377 |
ispartof | European food research & technology, 2024-05, Vol.250 (5), p.1401-1416 |
issn | 1438-2377 1438-2385 |
language | eng |
recordid | cdi_proquest_miscellaneous_3153626678 |
source | Springer Nature - Complete Springer Journals |
subjects | Agriculture Ammonium Ammonium sulfate Analytical Chemistry antibacterial properties Bacillus cereus Biological activity Biotechnology calcium Calcium ions Calotropis procera Cell walls Cellulose Chemistry Chemistry and Materials Science Chromatography Column chromatography E coli enzyme activity Enzymes Escherichia coli Food Science Forestry gel chromatography Gel filtration Ion exchange Latex Lysozyme Magnesium Metal ions molecular weight Original Paper pain Pharmaceutical industry Purification traditional medicine |
title | Extraction, purification, and investigation of the antibacterial potential of lysozyme isolated from the latex of Calotropis procera |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T08%3A47%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Extraction,%20purification,%20and%20investigation%20of%20the%20antibacterial%20potential%20of%20lysozyme%20isolated%20from%20the%20latex%20of%20Calotropis%20procera&rft.jtitle=European%20food%20research%20&%20technology&rft.au=Muthu,%20Sakthivel&rft.date=2024-05-01&rft.volume=250&rft.issue=5&rft.spage=1401&rft.epage=1416&rft.pages=1401-1416&rft.issn=1438-2377&rft.eissn=1438-2385&rft_id=info:doi/10.1007/s00217-024-04467-2&rft_dat=%3Cproquest_cross%3E3153626678%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2973573960&rft_id=info:pmid/&rfr_iscdi=true |