Extraction, purification, and investigation of the antibacterial potential of lysozyme isolated from the latex of Calotropis procera

Calotropis procera produces a latex used in traditional medicine because of its components which are found to be biologically active. The latex of C. procera has many benefits such as pain reduction, antimicrobial attitude and other precious attributes. Latex lysozyme (LL) is a particular protein co...

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Veröffentlicht in:European food research & technology 2024-05, Vol.250 (5), p.1401-1416
Hauptverfasser: Muthu, Sakthivel, Gopal, Venkateshbabu, AL-Younis, Zena Kadhim, Altemimi, Ammar B., Perumal, Palani, Lakshmikanthan, Mythileeswari, Krishnan, Kathiravan, Najm, Mazin A. A., Giuffrè, Angelo Maria, Govindan, Lakshmanan
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container_issue 5
container_start_page 1401
container_title European food research & technology
container_volume 250
creator Muthu, Sakthivel
Gopal, Venkateshbabu
AL-Younis, Zena Kadhim
Altemimi, Ammar B.
Perumal, Palani
Lakshmikanthan, Mythileeswari
Krishnan, Kathiravan
Najm, Mazin A. A.
Giuffrè, Angelo Maria
Govindan, Lakshmanan
description Calotropis procera produces a latex used in traditional medicine because of its components which are found to be biologically active. The latex of C. procera has many benefits such as pain reduction, antimicrobial attitude and other precious attributes. Latex lysozyme (LL) is a particular protein contained in C. procera plant having a promising antibacterial property. The process applied for extraction implicated ammonium sulfate precipitation, water extraction, ion exchange and gel filtration column chromatography evidencing 14.4 kDa for LL as a molecular mass. LL after purification showed 397.18 U mg −1 as specific activity (10.30 purification fold), and with 37.9% as a yield. In addition, the chemical and physical analyses showed that LL had its best realization when the pH was 5.5 at 50 °C. Moreover when metal ions such as Ca 2+ and Mg 2+ were incorporated the relative activity of the enzyme resulted ampliflied. When a purification was conducted with CM-Cellulose and Sephadex G-100 chromatography, LL revealed an antibacterial property versus both Escherichia coli (MICs 14 µg ml −1 ) and Bacillus cereus (MICs 13 µg ml −1 ). HR-TEM analysis displayed an antimicrobial potential of LL after its purification; that analysis showed the deterioration of the cell wall (external membrane of the cell) of both E. coli and B. cereus . Therefore, the LL obtained from C. procera maintains ability as an antibacterial enzyme applicable in various domains such as biotechnological and pharmaceutical industries. Graphical abstract
doi_str_mv 10.1007/s00217-024-04467-2
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The process applied for extraction implicated ammonium sulfate precipitation, water extraction, ion exchange and gel filtration column chromatography evidencing 14.4 kDa for LL as a molecular mass. LL after purification showed 397.18 U mg −1 as specific activity (10.30 purification fold), and with 37.9% as a yield. In addition, the chemical and physical analyses showed that LL had its best realization when the pH was 5.5 at 50 °C. Moreover when metal ions such as Ca 2+ and Mg 2+ were incorporated the relative activity of the enzyme resulted ampliflied. When a purification was conducted with CM-Cellulose and Sephadex G-100 chromatography, LL revealed an antibacterial property versus both Escherichia coli (MICs 14 µg ml −1 ) and Bacillus cereus (MICs 13 µg ml −1 ). HR-TEM analysis displayed an antimicrobial potential of LL after its purification; that analysis showed the deterioration of the cell wall (external membrane of the cell) of both E. coli and B. cereus . 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source Springer Nature - Complete Springer Journals
subjects Agriculture
Ammonium
Ammonium sulfate
Analytical Chemistry
antibacterial properties
Bacillus cereus
Biological activity
Biotechnology
calcium
Calcium ions
Calotropis procera
Cell walls
Cellulose
Chemistry
Chemistry and Materials Science
Chromatography
Column chromatography
E coli
enzyme activity
Enzymes
Escherichia coli
Food Science
Forestry
gel chromatography
Gel filtration
Ion exchange
Latex
Lysozyme
Magnesium
Metal ions
molecular weight
Original Paper
pain
Pharmaceutical industry
Purification
traditional medicine
title Extraction, purification, and investigation of the antibacterial potential of lysozyme isolated from the latex of Calotropis procera
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