Functional analysis of adipokinetic hormone signaling in reproductive diapause of Coccinella septempunctata
BACKGROUND The ladybeetle, Coccinella septempunctata, an important predator, is widely used to control aphids, whiteflies, mites, thrips, and lepidopteran pests. Diapause control technology is key to extending C. septempunctata shelf‐life and commercialization. Lipid accumulation is a major feature...
Gespeichert in:
| Veröffentlicht in: | Pest management science 2024-07, Vol.80 (7), p.3665-3674 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3674 |
|---|---|
| container_issue | 7 |
| container_start_page | 3665 |
| container_title | Pest management science |
| container_volume | 80 |
| creator | Shen, Zhongjian Liu, Zhaohan Chen, Junjie Li, Yuyan Mao, Jianjun Wang, Mengqing Zhang, Lisheng |
| description | BACKGROUND
The ladybeetle, Coccinella septempunctata, an important predator, is widely used to control aphids, whiteflies, mites, thrips, and lepidopteran pests. Diapause control technology is key to extending C. septempunctata shelf‐life and commercialization. Lipid accumulation is a major feature of reproductive diapause, but the function of AKH signaling as a regulator of lipid mobilization in reproductive diapause remains unclear. This study aimed to identify and characterize AKH and AKHR genes, and clarify their functions in reproductive diapause.
RESULTS
The relative expression levels of CsAKH and CsAKHR were the highest in the head and fat body, respectively, and were significantly decreased under diapause conditions, both in developmental stages and tissues (head, midgut, fat body, and ovary). Furthermore, CsAKH and CsAKHR expression was increased significantly after juvenile hormone (JH) injection, but CsMet silencing significantly inhibited CsAKH and CsAKHR expression, whereas CsMet knockdown blocked the induction effect of JH. CsAKH and CsAKHR knockdown significantly reduced water content, increased lipid storage, and promoted the expression of genes related to lipid synthesis, but significantly blocked ovarian development, and induced forkhead box O (FOXO) gene expression in C. septempunctata under reproduction conditions. By contrast, injection of AKH peptide significantly inhibited FOXO expression, reduced lipid storage, and increased water content in C. septempunctata under diapause conditions.
CONCLUSION
These results indicate that CsAKH and CsAKHR are involved in the regulation of lipid accumulation and ovarian development during diapause in C. septempunctata, and provide a promising target for manipulating C. septempunctata diapause. © 2024 Society of Chemical Industry.
CsAKH and CsAKHR expression is positively regulated by juvenile hormone and may be involved in reproductive diapause in Coccinella septempunctata by regulating FOXO expression. Our study provides a promising target for manipulating C. septempunctata diapause to facilitate large‐scale rearing and long‐term storage. |
| doi_str_mv | 10.1002/ps.8070 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153626253</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2954775845</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3400-f85cf6ca30ebfd4a277d10445922f55d91949181a2f4b1b8b1aedbe6ab887e7a3</originalsourceid><addsrcrecordid>eNqFkUtLxDAUhYMovvEfSMCFgsyYZ5ssZfAFgoIK7kqa3mq0bWrTKvPvTZ3RhSBucrP4zrmHexDao2RKCWEnbZgqkpIVtEklSyZCa7X681ePG2grhBdCiNaaraMNroTUWvBN9Ho-NLZ3vjEVNvGZBxewL7EpXOtfXQO9s_jZd7VvAAf3FBHXPGHX4A7azhdDFL8DLpxpzRBglM68tVFYVQYHaHuo23GF6c0OWitNFWB3ObfRw_nZ_exycn1zcTU7vZ5YLgiZlEraMrGGE8jLQhiWpgUlIiZmrJSy0FQLTRU1rBQ5zVVODRQ5JCZXKoXU8G10tPCNAd8GCH1Wu2DHQA34IWScSp6whEn-L8q0FGkq47kievALffFDF-8RDUkiEkETPlKHC8p2PoQOyqztXG26eUZJNlaVtSEbq4rk_tJvyGsofrjvbiJwvAA-XAXzv3yy27svu0_z_51p</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3064641635</pqid></control><display><type>article</type><title>Functional analysis of adipokinetic hormone signaling in reproductive diapause of Coccinella septempunctata</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Shen, Zhongjian ; Liu, Zhaohan ; Chen, Junjie ; Li, Yuyan ; Mao, Jianjun ; Wang, Mengqing ; Zhang, Lisheng</creator><creatorcontrib>Shen, Zhongjian ; Liu, Zhaohan ; Chen, Junjie ; Li, Yuyan ; Mao, Jianjun ; Wang, Mengqing ; Zhang, Lisheng</creatorcontrib><description>BACKGROUND
The ladybeetle, Coccinella septempunctata, an important predator, is widely used to control aphids, whiteflies, mites, thrips, and lepidopteran pests. Diapause control technology is key to extending C. septempunctata shelf‐life and commercialization. Lipid accumulation is a major feature of reproductive diapause, but the function of AKH signaling as a regulator of lipid mobilization in reproductive diapause remains unclear. This study aimed to identify and characterize AKH and AKHR genes, and clarify their functions in reproductive diapause.
RESULTS
The relative expression levels of CsAKH and CsAKHR were the highest in the head and fat body, respectively, and were significantly decreased under diapause conditions, both in developmental stages and tissues (head, midgut, fat body, and ovary). Furthermore, CsAKH and CsAKHR expression was increased significantly after juvenile hormone (JH) injection, but CsMet silencing significantly inhibited CsAKH and CsAKHR expression, whereas CsMet knockdown blocked the induction effect of JH. CsAKH and CsAKHR knockdown significantly reduced water content, increased lipid storage, and promoted the expression of genes related to lipid synthesis, but significantly blocked ovarian development, and induced forkhead box O (FOXO) gene expression in C. septempunctata under reproduction conditions. By contrast, injection of AKH peptide significantly inhibited FOXO expression, reduced lipid storage, and increased water content in C. septempunctata under diapause conditions.
CONCLUSION
These results indicate that CsAKH and CsAKHR are involved in the regulation of lipid accumulation and ovarian development during diapause in C. septempunctata, and provide a promising target for manipulating C. septempunctata diapause. © 2024 Society of Chemical Industry.
CsAKH and CsAKHR expression is positively regulated by juvenile hormone and may be involved in reproductive diapause in Coccinella septempunctata by regulating FOXO expression. Our study provides a promising target for manipulating C. septempunctata diapause to facilitate large‐scale rearing and long‐term storage.</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.8070</identifier><identifier>PMID: 38459943</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Accumulation ; adipokinetic hormone ; adipokinetic hormone receptor ; Animals ; Coccinella septempunctata ; Coleoptera - genetics ; Coleoptera - growth & development ; Coleoptera - metabolism ; Coleoptera - physiology ; Commercialization ; Developmental stages ; Diapause ; Diapause, Insect ; Fat body ; Female ; Forkhead protein ; Functional analysis ; Gene expression ; Genes ; head ; Injection ; Insect Hormones - genetics ; Insect Hormones - metabolism ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Juvenile hormones ; Lepidoptera ; Lipid Metabolism ; lipid mobilization ; Lipids ; Midgut ; Moisture content ; Oligopeptides - metabolism ; ovarian development ; Ovaries ; Pest control ; pest management ; Pests ; Pyrrolidonecarboxylic Acid - analogs & derivatives ; Pyrrolidonecarboxylic Acid - metabolism ; Reproduction ; reproductive diapause ; shelf life ; Signal Transduction ; Water content</subject><ispartof>Pest management science, 2024-07, Vol.80 (7), p.3665-3674</ispartof><rights>2024 Society of Chemical Industry.</rights><rights>Copyright © 2024 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3400-f85cf6ca30ebfd4a277d10445922f55d91949181a2f4b1b8b1aedbe6ab887e7a3</cites><orcidid>0000-0002-3045-5845</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.8070$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.8070$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38459943$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, Zhongjian</creatorcontrib><creatorcontrib>Liu, Zhaohan</creatorcontrib><creatorcontrib>Chen, Junjie</creatorcontrib><creatorcontrib>Li, Yuyan</creatorcontrib><creatorcontrib>Mao, Jianjun</creatorcontrib><creatorcontrib>Wang, Mengqing</creatorcontrib><creatorcontrib>Zhang, Lisheng</creatorcontrib><title>Functional analysis of adipokinetic hormone signaling in reproductive diapause of Coccinella septempunctata</title><title>Pest management science</title><addtitle>Pest Manag Sci</addtitle><description>BACKGROUND
The ladybeetle, Coccinella septempunctata, an important predator, is widely used to control aphids, whiteflies, mites, thrips, and lepidopteran pests. Diapause control technology is key to extending C. septempunctata shelf‐life and commercialization. Lipid accumulation is a major feature of reproductive diapause, but the function of AKH signaling as a regulator of lipid mobilization in reproductive diapause remains unclear. This study aimed to identify and characterize AKH and AKHR genes, and clarify their functions in reproductive diapause.
RESULTS
The relative expression levels of CsAKH and CsAKHR were the highest in the head and fat body, respectively, and were significantly decreased under diapause conditions, both in developmental stages and tissues (head, midgut, fat body, and ovary). Furthermore, CsAKH and CsAKHR expression was increased significantly after juvenile hormone (JH) injection, but CsMet silencing significantly inhibited CsAKH and CsAKHR expression, whereas CsMet knockdown blocked the induction effect of JH. CsAKH and CsAKHR knockdown significantly reduced water content, increased lipid storage, and promoted the expression of genes related to lipid synthesis, but significantly blocked ovarian development, and induced forkhead box O (FOXO) gene expression in C. septempunctata under reproduction conditions. By contrast, injection of AKH peptide significantly inhibited FOXO expression, reduced lipid storage, and increased water content in C. septempunctata under diapause conditions.
CONCLUSION
These results indicate that CsAKH and CsAKHR are involved in the regulation of lipid accumulation and ovarian development during diapause in C. septempunctata, and provide a promising target for manipulating C. septempunctata diapause. © 2024 Society of Chemical Industry.
CsAKH and CsAKHR expression is positively regulated by juvenile hormone and may be involved in reproductive diapause in Coccinella septempunctata by regulating FOXO expression. Our study provides a promising target for manipulating C. septempunctata diapause to facilitate large‐scale rearing and long‐term storage.</description><subject>Accumulation</subject><subject>adipokinetic hormone</subject><subject>adipokinetic hormone receptor</subject><subject>Animals</subject><subject>Coccinella septempunctata</subject><subject>Coleoptera - genetics</subject><subject>Coleoptera - growth & development</subject><subject>Coleoptera - metabolism</subject><subject>Coleoptera - physiology</subject><subject>Commercialization</subject><subject>Developmental stages</subject><subject>Diapause</subject><subject>Diapause, Insect</subject><subject>Fat body</subject><subject>Female</subject><subject>Forkhead protein</subject><subject>Functional analysis</subject><subject>Gene expression</subject><subject>Genes</subject><subject>head</subject><subject>Injection</subject><subject>Insect Hormones - genetics</subject><subject>Insect Hormones - metabolism</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Juvenile hormones</subject><subject>Lepidoptera</subject><subject>Lipid Metabolism</subject><subject>lipid mobilization</subject><subject>Lipids</subject><subject>Midgut</subject><subject>Moisture content</subject><subject>Oligopeptides - metabolism</subject><subject>ovarian development</subject><subject>Ovaries</subject><subject>Pest control</subject><subject>pest management</subject><subject>Pests</subject><subject>Pyrrolidonecarboxylic Acid - analogs & derivatives</subject><subject>Pyrrolidonecarboxylic Acid - metabolism</subject><subject>Reproduction</subject><subject>reproductive diapause</subject><subject>shelf life</subject><subject>Signal Transduction</subject><subject>Water content</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLxDAUhYMovvEfSMCFgsyYZ5ssZfAFgoIK7kqa3mq0bWrTKvPvTZ3RhSBucrP4zrmHexDao2RKCWEnbZgqkpIVtEklSyZCa7X681ePG2grhBdCiNaaraMNroTUWvBN9Ho-NLZ3vjEVNvGZBxewL7EpXOtfXQO9s_jZd7VvAAf3FBHXPGHX4A7azhdDFL8DLpxpzRBglM68tVFYVQYHaHuo23GF6c0OWitNFWB3ObfRw_nZ_exycn1zcTU7vZ5YLgiZlEraMrGGE8jLQhiWpgUlIiZmrJSy0FQLTRU1rBQ5zVVODRQ5JCZXKoXU8G10tPCNAd8GCH1Wu2DHQA34IWScSp6whEn-L8q0FGkq47kievALffFDF-8RDUkiEkETPlKHC8p2PoQOyqztXG26eUZJNlaVtSEbq4rk_tJvyGsofrjvbiJwvAA-XAXzv3yy27svu0_z_51p</recordid><startdate>202407</startdate><enddate>202407</enddate><creator>Shen, Zhongjian</creator><creator>Liu, Zhaohan</creator><creator>Chen, Junjie</creator><creator>Li, Yuyan</creator><creator>Mao, Jianjun</creator><creator>Wang, Mengqing</creator><creator>Zhang, Lisheng</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-3045-5845</orcidid></search><sort><creationdate>202407</creationdate><title>Functional analysis of adipokinetic hormone signaling in reproductive diapause of Coccinella septempunctata</title><author>Shen, Zhongjian ; Liu, Zhaohan ; Chen, Junjie ; Li, Yuyan ; Mao, Jianjun ; Wang, Mengqing ; Zhang, Lisheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3400-f85cf6ca30ebfd4a277d10445922f55d91949181a2f4b1b8b1aedbe6ab887e7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Accumulation</topic><topic>adipokinetic hormone</topic><topic>adipokinetic hormone receptor</topic><topic>Animals</topic><topic>Coccinella septempunctata</topic><topic>Coleoptera - genetics</topic><topic>Coleoptera - growth & development</topic><topic>Coleoptera - metabolism</topic><topic>Coleoptera - physiology</topic><topic>Commercialization</topic><topic>Developmental stages</topic><topic>Diapause</topic><topic>Diapause, Insect</topic><topic>Fat body</topic><topic>Female</topic><topic>Forkhead protein</topic><topic>Functional analysis</topic><topic>Gene expression</topic><topic>Genes</topic><topic>head</topic><topic>Injection</topic><topic>Insect Hormones - genetics</topic><topic>Insect Hormones - metabolism</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>Juvenile hormones</topic><topic>Lepidoptera</topic><topic>Lipid Metabolism</topic><topic>lipid mobilization</topic><topic>Lipids</topic><topic>Midgut</topic><topic>Moisture content</topic><topic>Oligopeptides - metabolism</topic><topic>ovarian development</topic><topic>Ovaries</topic><topic>Pest control</topic><topic>pest management</topic><topic>Pests</topic><topic>Pyrrolidonecarboxylic Acid - analogs & derivatives</topic><topic>Pyrrolidonecarboxylic Acid - metabolism</topic><topic>Reproduction</topic><topic>reproductive diapause</topic><topic>shelf life</topic><topic>Signal Transduction</topic><topic>Water content</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shen, Zhongjian</creatorcontrib><creatorcontrib>Liu, Zhaohan</creatorcontrib><creatorcontrib>Chen, Junjie</creatorcontrib><creatorcontrib>Li, Yuyan</creatorcontrib><creatorcontrib>Mao, Jianjun</creatorcontrib><creatorcontrib>Wang, Mengqing</creatorcontrib><creatorcontrib>Zhang, Lisheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shen, Zhongjian</au><au>Liu, Zhaohan</au><au>Chen, Junjie</au><au>Li, Yuyan</au><au>Mao, Jianjun</au><au>Wang, Mengqing</au><au>Zhang, Lisheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional analysis of adipokinetic hormone signaling in reproductive diapause of Coccinella septempunctata</atitle><jtitle>Pest management science</jtitle><addtitle>Pest Manag Sci</addtitle><date>2024-07</date><risdate>2024</risdate><volume>80</volume><issue>7</issue><spage>3665</spage><epage>3674</epage><pages>3665-3674</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>BACKGROUND
The ladybeetle, Coccinella septempunctata, an important predator, is widely used to control aphids, whiteflies, mites, thrips, and lepidopteran pests. Diapause control technology is key to extending C. septempunctata shelf‐life and commercialization. Lipid accumulation is a major feature of reproductive diapause, but the function of AKH signaling as a regulator of lipid mobilization in reproductive diapause remains unclear. This study aimed to identify and characterize AKH and AKHR genes, and clarify their functions in reproductive diapause.
RESULTS
The relative expression levels of CsAKH and CsAKHR were the highest in the head and fat body, respectively, and were significantly decreased under diapause conditions, both in developmental stages and tissues (head, midgut, fat body, and ovary). Furthermore, CsAKH and CsAKHR expression was increased significantly after juvenile hormone (JH) injection, but CsMet silencing significantly inhibited CsAKH and CsAKHR expression, whereas CsMet knockdown blocked the induction effect of JH. CsAKH and CsAKHR knockdown significantly reduced water content, increased lipid storage, and promoted the expression of genes related to lipid synthesis, but significantly blocked ovarian development, and induced forkhead box O (FOXO) gene expression in C. septempunctata under reproduction conditions. By contrast, injection of AKH peptide significantly inhibited FOXO expression, reduced lipid storage, and increased water content in C. septempunctata under diapause conditions.
CONCLUSION
These results indicate that CsAKH and CsAKHR are involved in the regulation of lipid accumulation and ovarian development during diapause in C. septempunctata, and provide a promising target for manipulating C. septempunctata diapause. © 2024 Society of Chemical Industry.
CsAKH and CsAKHR expression is positively regulated by juvenile hormone and may be involved in reproductive diapause in Coccinella septempunctata by regulating FOXO expression. Our study provides a promising target for manipulating C. septempunctata diapause to facilitate large‐scale rearing and long‐term storage.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>38459943</pmid><doi>10.1002/ps.8070</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-3045-5845</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1526-498X |
| ispartof | Pest management science, 2024-07, Vol.80 (7), p.3665-3674 |
| issn | 1526-498X 1526-4998 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3153626253 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Accumulation adipokinetic hormone adipokinetic hormone receptor Animals Coccinella septempunctata Coleoptera - genetics Coleoptera - growth & development Coleoptera - metabolism Coleoptera - physiology Commercialization Developmental stages Diapause Diapause, Insect Fat body Female Forkhead protein Functional analysis Gene expression Genes head Injection Insect Hormones - genetics Insect Hormones - metabolism Insect Proteins - genetics Insect Proteins - metabolism Juvenile hormones Lepidoptera Lipid Metabolism lipid mobilization Lipids Midgut Moisture content Oligopeptides - metabolism ovarian development Ovaries Pest control pest management Pests Pyrrolidonecarboxylic Acid - analogs & derivatives Pyrrolidonecarboxylic Acid - metabolism Reproduction reproductive diapause shelf life Signal Transduction Water content |
| title | Functional analysis of adipokinetic hormone signaling in reproductive diapause of Coccinella septempunctata |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T19%3A45%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20analysis%20of%20adipokinetic%20hormone%20signaling%20in%20reproductive%20diapause%20of%20Coccinella%20septempunctata&rft.jtitle=Pest%20management%20science&rft.au=Shen,%20Zhongjian&rft.date=2024-07&rft.volume=80&rft.issue=7&rft.spage=3665&rft.epage=3674&rft.pages=3665-3674&rft.issn=1526-498X&rft.eissn=1526-4998&rft_id=info:doi/10.1002/ps.8070&rft_dat=%3Cproquest_cross%3E2954775845%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3064641635&rft_id=info:pmid/38459943&rfr_iscdi=true |