Quercetin inhibits neuronal Ferroptosis and promotes immune response by targeting lipid metabolism-related gene PTGS2 to alleviate breast cancer-related depression
Quercetin, the key ingredient in Xiaoyao Kangai Jieyu Formula, has been previously found to relieve breast cancer-related depression (BCRD). We want to explore the potential mechanisms and therapeutic targets of quercetin alleviating BCRD. BALB/c mice were injected subcutaneously with 4T1 cells and...
Gespeichert in:
| Veröffentlicht in: | Phytomedicine (Stuttgart) 2024-07, Vol.130, p.155560, Article 155560 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | 155560 |
| container_title | Phytomedicine (Stuttgart) |
| container_volume | 130 |
| creator | Zhu, Qing Han, Yuanshan He, Ying Meng, Pan Fu, Yilan Yang, Hui He, Gefei Long, Minghui Shi, Yingrui |
| description | Quercetin, the key ingredient in Xiaoyao Kangai Jieyu Formula, has been previously found to relieve breast cancer-related depression (BCRD).
We want to explore the potential mechanisms and therapeutic targets of quercetin alleviating BCRD.
BALB/c mice were injected subcutaneously with 4T1 cells and corticosterone (CORT) to create a BCRD mice model. The primary hippocampal neurons were co-induced with 10 μg/ml lipopolysaccharide (LPS) and 200 μM CORT for 6 h to establish an in vitro model of BCRD. Quercetin was applied to explore its effect on disease symptoms, gut microbiota, and lipid metabolism of BCRD mice. Lipid metabolism-related genes were screened based on network pharmacology. Molecular docking was employed to prove whether quercetin bound to prostaglandin-endoperoxide synthase 2 (PTGS2). PTGS2 overexpression was carried out to explore the underlying mechanism of quercetin treatment on BCRD.
Quercetin treatment not only altered the composition and abundance of gut microbiota but also alleviated abnormal lipid metabolism in BCRD mice. In particular, quercetin down-regulated BCRD and lipid metabolism-related genes screened by network pharmacology, especially PTGS2. Further, molecular docking verified the stable binding between quercetin and PTGS2. In hippocampal neurons, quercetin promoted proliferation but reduced ferroptosis-related markers (total Fe, Fe2+, MDA, and ROS) levels by targeting PTGS2. In BCRD mice, quercetin reduced the high immobility time and increased the sucrose preference rate and serotonin (5-HT), dopamine (DA), and noradrenaline (NE) levels. Meanwhile, quercetin increased CD4+/CD8+ T cells ratio and IL-2 and IFN-γ levels but reduced CA153 and IL-10 levels to alleviate BCRD development. However, PTGS2 overexpression reversed these effects of quercetin on BCRD.
Quercetin inhibited neuronal ferroptosis and promoted immune responses in BCRD mice by targeting the lipid metabolism-related gene PTGS2. This provided a reference for quercetin in the treatment of BCRD.
[Display omitted] |
| doi_str_mv | 10.1016/j.phymed.2024.155560 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153623887</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0944711324002253</els_id><sourcerecordid>3153623887</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-708b1786d3691952d5e08b3149aba429889ab488b20fe0feaf9d7bf084053fe53</originalsourceid><addsrcrecordid>eNqFkdFqHCEUhqW0NNu0b1CKl72ZrY4649wUSmjSQCANSaF34oxnNi6OTtUJ7PPkResySS5bEBTPd_7D-X-EPlKypYQ2X_bb-f4wgdnWpOZbKoRoyCu0oQ2VFenE79doQzrOq5ZSdoLepbQnhPKuJW_RCZOSCk74Bj3eLBAHyNZj6-9tb3PCHpYYvHb4HGIMcw7JJqy9wXMMU8iQsJ2mxQOOkObgE-D-gLOOu6PMDjs7W4MnyLoPzqapiuB0BoN3UHp-3l3c1jgHrJ2DB1sKuI-gU8aD9gPEF9rAXPSTDf49ejNql-DD032Kfp1_vzv7UV1dX1yefbuqBsZ5rloie9rKxrCmo52ojYDyw8rOute87qQsDy5lX5MRytFjZ9p-JJITwUYQ7BR9XnXLnn8WSFlNNg3gnPYQlqQYFaypi3ft_1HSMC5kW9OC8hUdYkgpwqjmaCcdD4oSdUxS7dWapDomqdYkS9unpwlLf6w9Nz1HV4CvKwDFkgcLUaXBQrHQ2AhDVibYf0_4C8E5tL4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3063458721</pqid></control><display><type>article</type><title>Quercetin inhibits neuronal Ferroptosis and promotes immune response by targeting lipid metabolism-related gene PTGS2 to alleviate breast cancer-related depression</title><source>Elsevier ScienceDirect Journals</source><creator>Zhu, Qing ; Han, Yuanshan ; He, Ying ; Meng, Pan ; Fu, Yilan ; Yang, Hui ; He, Gefei ; Long, Minghui ; Shi, Yingrui</creator><creatorcontrib>Zhu, Qing ; Han, Yuanshan ; He, Ying ; Meng, Pan ; Fu, Yilan ; Yang, Hui ; He, Gefei ; Long, Minghui ; Shi, Yingrui</creatorcontrib><description>Quercetin, the key ingredient in Xiaoyao Kangai Jieyu Formula, has been previously found to relieve breast cancer-related depression (BCRD).
We want to explore the potential mechanisms and therapeutic targets of quercetin alleviating BCRD.
BALB/c mice were injected subcutaneously with 4T1 cells and corticosterone (CORT) to create a BCRD mice model. The primary hippocampal neurons were co-induced with 10 μg/ml lipopolysaccharide (LPS) and 200 μM CORT for 6 h to establish an in vitro model of BCRD. Quercetin was applied to explore its effect on disease symptoms, gut microbiota, and lipid metabolism of BCRD mice. Lipid metabolism-related genes were screened based on network pharmacology. Molecular docking was employed to prove whether quercetin bound to prostaglandin-endoperoxide synthase 2 (PTGS2). PTGS2 overexpression was carried out to explore the underlying mechanism of quercetin treatment on BCRD.
Quercetin treatment not only altered the composition and abundance of gut microbiota but also alleviated abnormal lipid metabolism in BCRD mice. In particular, quercetin down-regulated BCRD and lipid metabolism-related genes screened by network pharmacology, especially PTGS2. Further, molecular docking verified the stable binding between quercetin and PTGS2. In hippocampal neurons, quercetin promoted proliferation but reduced ferroptosis-related markers (total Fe, Fe2+, MDA, and ROS) levels by targeting PTGS2. In BCRD mice, quercetin reduced the high immobility time and increased the sucrose preference rate and serotonin (5-HT), dopamine (DA), and noradrenaline (NE) levels. Meanwhile, quercetin increased CD4+/CD8+ T cells ratio and IL-2 and IFN-γ levels but reduced CA153 and IL-10 levels to alleviate BCRD development. However, PTGS2 overexpression reversed these effects of quercetin on BCRD.
Quercetin inhibited neuronal ferroptosis and promoted immune responses in BCRD mice by targeting the lipid metabolism-related gene PTGS2. This provided a reference for quercetin in the treatment of BCRD.
[Display omitted]</description><identifier>ISSN: 0944-7113</identifier><identifier>ISSN: 1618-095X</identifier><identifier>EISSN: 1618-095X</identifier><identifier>DOI: 10.1016/j.phymed.2024.155560</identifier><identifier>PMID: 38815404</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Breast cancer-related depression ; breasts ; corticosterone ; dopamine ; Ferroptosis ; genes ; Gut microbiota ; immune response ; ingredients ; interleukin-10 ; interleukin-2 ; intestinal microorganisms ; iron ; Lipid metabolism ; lipopolysaccharides ; Molecular docking ; Network pharmacology ; neurons ; norepinephrine ; pharmacology ; prostaglandin synthase ; Quercetin ; serotonin ; sucrose ; therapeutics</subject><ispartof>Phytomedicine (Stuttgart), 2024-07, Vol.130, p.155560, Article 155560</ispartof><rights>2024</rights><rights>Copyright © 2024. Published by Elsevier GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c344t-708b1786d3691952d5e08b3149aba429889ab488b20fe0feaf9d7bf084053fe53</cites><orcidid>0000-0002-2057-9422</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0944711324002253$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38815404$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Qing</creatorcontrib><creatorcontrib>Han, Yuanshan</creatorcontrib><creatorcontrib>He, Ying</creatorcontrib><creatorcontrib>Meng, Pan</creatorcontrib><creatorcontrib>Fu, Yilan</creatorcontrib><creatorcontrib>Yang, Hui</creatorcontrib><creatorcontrib>He, Gefei</creatorcontrib><creatorcontrib>Long, Minghui</creatorcontrib><creatorcontrib>Shi, Yingrui</creatorcontrib><title>Quercetin inhibits neuronal Ferroptosis and promotes immune response by targeting lipid metabolism-related gene PTGS2 to alleviate breast cancer-related depression</title><title>Phytomedicine (Stuttgart)</title><addtitle>Phytomedicine</addtitle><description>Quercetin, the key ingredient in Xiaoyao Kangai Jieyu Formula, has been previously found to relieve breast cancer-related depression (BCRD).
We want to explore the potential mechanisms and therapeutic targets of quercetin alleviating BCRD.
BALB/c mice were injected subcutaneously with 4T1 cells and corticosterone (CORT) to create a BCRD mice model. The primary hippocampal neurons were co-induced with 10 μg/ml lipopolysaccharide (LPS) and 200 μM CORT for 6 h to establish an in vitro model of BCRD. Quercetin was applied to explore its effect on disease symptoms, gut microbiota, and lipid metabolism of BCRD mice. Lipid metabolism-related genes were screened based on network pharmacology. Molecular docking was employed to prove whether quercetin bound to prostaglandin-endoperoxide synthase 2 (PTGS2). PTGS2 overexpression was carried out to explore the underlying mechanism of quercetin treatment on BCRD.
Quercetin treatment not only altered the composition and abundance of gut microbiota but also alleviated abnormal lipid metabolism in BCRD mice. In particular, quercetin down-regulated BCRD and lipid metabolism-related genes screened by network pharmacology, especially PTGS2. Further, molecular docking verified the stable binding between quercetin and PTGS2. In hippocampal neurons, quercetin promoted proliferation but reduced ferroptosis-related markers (total Fe, Fe2+, MDA, and ROS) levels by targeting PTGS2. In BCRD mice, quercetin reduced the high immobility time and increased the sucrose preference rate and serotonin (5-HT), dopamine (DA), and noradrenaline (NE) levels. Meanwhile, quercetin increased CD4+/CD8+ T cells ratio and IL-2 and IFN-γ levels but reduced CA153 and IL-10 levels to alleviate BCRD development. However, PTGS2 overexpression reversed these effects of quercetin on BCRD.
Quercetin inhibited neuronal ferroptosis and promoted immune responses in BCRD mice by targeting the lipid metabolism-related gene PTGS2. This provided a reference for quercetin in the treatment of BCRD.
[Display omitted]</description><subject>Breast cancer-related depression</subject><subject>breasts</subject><subject>corticosterone</subject><subject>dopamine</subject><subject>Ferroptosis</subject><subject>genes</subject><subject>Gut microbiota</subject><subject>immune response</subject><subject>ingredients</subject><subject>interleukin-10</subject><subject>interleukin-2</subject><subject>intestinal microorganisms</subject><subject>iron</subject><subject>Lipid metabolism</subject><subject>lipopolysaccharides</subject><subject>Molecular docking</subject><subject>Network pharmacology</subject><subject>neurons</subject><subject>norepinephrine</subject><subject>pharmacology</subject><subject>prostaglandin synthase</subject><subject>Quercetin</subject><subject>serotonin</subject><subject>sucrose</subject><subject>therapeutics</subject><issn>0944-7113</issn><issn>1618-095X</issn><issn>1618-095X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkdFqHCEUhqW0NNu0b1CKl72ZrY4649wUSmjSQCANSaF34oxnNi6OTtUJ7PPkResySS5bEBTPd_7D-X-EPlKypYQ2X_bb-f4wgdnWpOZbKoRoyCu0oQ2VFenE79doQzrOq5ZSdoLepbQnhPKuJW_RCZOSCk74Bj3eLBAHyNZj6-9tb3PCHpYYvHb4HGIMcw7JJqy9wXMMU8iQsJ2mxQOOkObgE-D-gLOOu6PMDjs7W4MnyLoPzqapiuB0BoN3UHp-3l3c1jgHrJ2DB1sKuI-gU8aD9gPEF9rAXPSTDf49ejNql-DD032Kfp1_vzv7UV1dX1yefbuqBsZ5rloie9rKxrCmo52ojYDyw8rOute87qQsDy5lX5MRytFjZ9p-JJITwUYQ7BR9XnXLnn8WSFlNNg3gnPYQlqQYFaypi3ft_1HSMC5kW9OC8hUdYkgpwqjmaCcdD4oSdUxS7dWapDomqdYkS9unpwlLf6w9Nz1HV4CvKwDFkgcLUaXBQrHQ2AhDVibYf0_4C8E5tL4</recordid><startdate>20240725</startdate><enddate>20240725</enddate><creator>Zhu, Qing</creator><creator>Han, Yuanshan</creator><creator>He, Ying</creator><creator>Meng, Pan</creator><creator>Fu, Yilan</creator><creator>Yang, Hui</creator><creator>He, Gefei</creator><creator>Long, Minghui</creator><creator>Shi, Yingrui</creator><general>Elsevier GmbH</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-2057-9422</orcidid></search><sort><creationdate>20240725</creationdate><title>Quercetin inhibits neuronal Ferroptosis and promotes immune response by targeting lipid metabolism-related gene PTGS2 to alleviate breast cancer-related depression</title><author>Zhu, Qing ; Han, Yuanshan ; He, Ying ; Meng, Pan ; Fu, Yilan ; Yang, Hui ; He, Gefei ; Long, Minghui ; Shi, Yingrui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-708b1786d3691952d5e08b3149aba429889ab488b20fe0feaf9d7bf084053fe53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Breast cancer-related depression</topic><topic>breasts</topic><topic>corticosterone</topic><topic>dopamine</topic><topic>Ferroptosis</topic><topic>genes</topic><topic>Gut microbiota</topic><topic>immune response</topic><topic>ingredients</topic><topic>interleukin-10</topic><topic>interleukin-2</topic><topic>intestinal microorganisms</topic><topic>iron</topic><topic>Lipid metabolism</topic><topic>lipopolysaccharides</topic><topic>Molecular docking</topic><topic>Network pharmacology</topic><topic>neurons</topic><topic>norepinephrine</topic><topic>pharmacology</topic><topic>prostaglandin synthase</topic><topic>Quercetin</topic><topic>serotonin</topic><topic>sucrose</topic><topic>therapeutics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Qing</creatorcontrib><creatorcontrib>Han, Yuanshan</creatorcontrib><creatorcontrib>He, Ying</creatorcontrib><creatorcontrib>Meng, Pan</creatorcontrib><creatorcontrib>Fu, Yilan</creatorcontrib><creatorcontrib>Yang, Hui</creatorcontrib><creatorcontrib>He, Gefei</creatorcontrib><creatorcontrib>Long, Minghui</creatorcontrib><creatorcontrib>Shi, Yingrui</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Phytomedicine (Stuttgart)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Qing</au><au>Han, Yuanshan</au><au>He, Ying</au><au>Meng, Pan</au><au>Fu, Yilan</au><au>Yang, Hui</au><au>He, Gefei</au><au>Long, Minghui</au><au>Shi, Yingrui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quercetin inhibits neuronal Ferroptosis and promotes immune response by targeting lipid metabolism-related gene PTGS2 to alleviate breast cancer-related depression</atitle><jtitle>Phytomedicine (Stuttgart)</jtitle><addtitle>Phytomedicine</addtitle><date>2024-07-25</date><risdate>2024</risdate><volume>130</volume><spage>155560</spage><pages>155560-</pages><artnum>155560</artnum><issn>0944-7113</issn><issn>1618-095X</issn><eissn>1618-095X</eissn><abstract>Quercetin, the key ingredient in Xiaoyao Kangai Jieyu Formula, has been previously found to relieve breast cancer-related depression (BCRD).
We want to explore the potential mechanisms and therapeutic targets of quercetin alleviating BCRD.
BALB/c mice were injected subcutaneously with 4T1 cells and corticosterone (CORT) to create a BCRD mice model. The primary hippocampal neurons were co-induced with 10 μg/ml lipopolysaccharide (LPS) and 200 μM CORT for 6 h to establish an in vitro model of BCRD. Quercetin was applied to explore its effect on disease symptoms, gut microbiota, and lipid metabolism of BCRD mice. Lipid metabolism-related genes were screened based on network pharmacology. Molecular docking was employed to prove whether quercetin bound to prostaglandin-endoperoxide synthase 2 (PTGS2). PTGS2 overexpression was carried out to explore the underlying mechanism of quercetin treatment on BCRD.
Quercetin treatment not only altered the composition and abundance of gut microbiota but also alleviated abnormal lipid metabolism in BCRD mice. In particular, quercetin down-regulated BCRD and lipid metabolism-related genes screened by network pharmacology, especially PTGS2. Further, molecular docking verified the stable binding between quercetin and PTGS2. In hippocampal neurons, quercetin promoted proliferation but reduced ferroptosis-related markers (total Fe, Fe2+, MDA, and ROS) levels by targeting PTGS2. In BCRD mice, quercetin reduced the high immobility time and increased the sucrose preference rate and serotonin (5-HT), dopamine (DA), and noradrenaline (NE) levels. Meanwhile, quercetin increased CD4+/CD8+ T cells ratio and IL-2 and IFN-γ levels but reduced CA153 and IL-10 levels to alleviate BCRD development. However, PTGS2 overexpression reversed these effects of quercetin on BCRD.
Quercetin inhibited neuronal ferroptosis and promoted immune responses in BCRD mice by targeting the lipid metabolism-related gene PTGS2. This provided a reference for quercetin in the treatment of BCRD.
[Display omitted]</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>38815404</pmid><doi>10.1016/j.phymed.2024.155560</doi><orcidid>https://orcid.org/0000-0002-2057-9422</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0944-7113 |
| ispartof | Phytomedicine (Stuttgart), 2024-07, Vol.130, p.155560, Article 155560 |
| issn | 0944-7113 1618-095X 1618-095X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3153623887 |
| source | Elsevier ScienceDirect Journals |
| subjects | Breast cancer-related depression breasts corticosterone dopamine Ferroptosis genes Gut microbiota immune response ingredients interleukin-10 interleukin-2 intestinal microorganisms iron Lipid metabolism lipopolysaccharides Molecular docking Network pharmacology neurons norepinephrine pharmacology prostaglandin synthase Quercetin serotonin sucrose therapeutics |
| title | Quercetin inhibits neuronal Ferroptosis and promotes immune response by targeting lipid metabolism-related gene PTGS2 to alleviate breast cancer-related depression |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T17%3A31%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quercetin%20inhibits%20neuronal%20Ferroptosis%20and%20promotes%20immune%20response%20by%20targeting%20lipid%20metabolism-related%20gene%20PTGS2%20to%20alleviate%20breast%20cancer-related%20depression&rft.jtitle=Phytomedicine%20(Stuttgart)&rft.au=Zhu,%20Qing&rft.date=2024-07-25&rft.volume=130&rft.spage=155560&rft.pages=155560-&rft.artnum=155560&rft.issn=0944-7113&rft.eissn=1618-095X&rft_id=info:doi/10.1016/j.phymed.2024.155560&rft_dat=%3Cproquest_cross%3E3153623887%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3063458721&rft_id=info:pmid/38815404&rft_els_id=S0944711324002253&rfr_iscdi=true |