Oxaloacetate as new inducer for osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells in vitro
Background Mitochondrial organelles play a crucial role in cellular metabolism so different cell types exhibit diverse metabolic and energy demands. Therefore, alternations in the intracellular distribution, quantity, function, and structure of mitochondria are required for stem cell differentiation...
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| Veröffentlicht in: | Molecular biology reports 2024-12, Vol.51 (1), p.451-451, Article 451 |
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| creator | Shirkoohi, Fatemeh Jamali Ghollasi, Marzieh Halabian, Raheleh Eftekhari, Elahe Ghiasi, Mohsen |
| description | Background
Mitochondrial organelles play a crucial role in cellular metabolism so different cell types exhibit diverse metabolic and energy demands. Therefore, alternations in the intracellular distribution, quantity, function, and structure of mitochondria are required for stem cell differentiation. Finding an effective inducer capable of modulating mitochondrial activity is critical for the differentiation of specific stem cells into osteo-like cells for addressing issues related to osteogenic disorders. This study aimed to investigate the effect of oxaloacetate (OAA) on the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADSCs) in vitro.
Methods and results
First, the most favorable OAA concentration was measured through MTT assay and subsequently confirmed using acridine orange staining. Human ADSCs were cultured in osteogenic medium supplemented with OAA and analyzed on days 7 and 14 of differentiation. Various assays including alkaline phosphatase assay (ALP), cellular calcium content assay, mineralized matrix staining with alizarin red, catalase (CAT) and superoxide dismutase (SOD) activity, and real-time RT-PCR analysis of three bone-specific markers (ALP, osteocalcin, and collagen type I) were conducted to characterize the differentiated cells. Following viability assessment, OAA at a concentration of 1 µM was considered the optimal dosage for further studies. The results of osteogenic differentiation assays showed that OAA at a concentration of 1 × 10
− 6
M significantly increased ALP enzyme activity, mineralization, CAT and SOD activity and the expression of bone-specific genes in differentiated cells compared to control groups in vitro.
Conclusions
In conclusion, the fundings from this study suggest that OAA possesses favorable properties that make it a potential candidate for application in medical bone regeneration. |
| doi_str_mv | 10.1007/s11033-024-09389-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3153622006</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3153622006</sourcerecordid><originalsourceid>FETCH-LOGICAL-c359t-3f849962ae8fa7c3a7809317a380f67f8103aae3d1937f5ca2fea350c27afe003</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi1ERbcLf4ADssSll9BxHMfOEVWUIlXqBc7R4IxbV4m92E6h_Hq8bAGJA5x88DPvfDyMvRTwRgDosywESNlA2zUwSDM0_RO2EUrLphu0eco2IEE0nVHimJ3kfAcAndDqGTuWRslegd6w79ffcI5oqWAhjpkH-sp9mFZLibuYeMyF4g0Fb_nknaNEoXgsPgYeHb9dFwwcJ7-LmXjxOa_UTJT8PU18oUzB3j4sOPOasnBL85xrOr_3JcXn7MjhnOnF47tlny7efTy_bK6u3384f3vVWKmG0khnumHoWyTjUFuJ2tRthUZpwPXamXoERJKTGKR2ymLrCKUC22p0BCC37PSQu0vxy0q5jIvP-1EwUFzzKEU9RtsC9P9HQXQAg6k33rLXf6F3cU2hLlKpqkX1rdz3bg-UTTHnRG7cJb9gehgFjHuJ40HiWCWOPyWO-ylePUavnxeafpf8slYBeQBy_Qo3lP70_kfsD5mzqGo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3003356230</pqid></control><display><type>article</type><title>Oxaloacetate as new inducer for osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells in vitro</title><source>SpringerLink Journals - AutoHoldings</source><creator>Shirkoohi, Fatemeh Jamali ; Ghollasi, Marzieh ; Halabian, Raheleh ; Eftekhari, Elahe ; Ghiasi, Mohsen</creator><creatorcontrib>Shirkoohi, Fatemeh Jamali ; Ghollasi, Marzieh ; Halabian, Raheleh ; Eftekhari, Elahe ; Ghiasi, Mohsen</creatorcontrib><description>Background
Mitochondrial organelles play a crucial role in cellular metabolism so different cell types exhibit diverse metabolic and energy demands. Therefore, alternations in the intracellular distribution, quantity, function, and structure of mitochondria are required for stem cell differentiation. Finding an effective inducer capable of modulating mitochondrial activity is critical for the differentiation of specific stem cells into osteo-like cells for addressing issues related to osteogenic disorders. This study aimed to investigate the effect of oxaloacetate (OAA) on the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADSCs) in vitro.
Methods and results
First, the most favorable OAA concentration was measured through MTT assay and subsequently confirmed using acridine orange staining. Human ADSCs were cultured in osteogenic medium supplemented with OAA and analyzed on days 7 and 14 of differentiation. Various assays including alkaline phosphatase assay (ALP), cellular calcium content assay, mineralized matrix staining with alizarin red, catalase (CAT) and superoxide dismutase (SOD) activity, and real-time RT-PCR analysis of three bone-specific markers (ALP, osteocalcin, and collagen type I) were conducted to characterize the differentiated cells. Following viability assessment, OAA at a concentration of 1 µM was considered the optimal dosage for further studies. The results of osteogenic differentiation assays showed that OAA at a concentration of 1 × 10
− 6
M significantly increased ALP enzyme activity, mineralization, CAT and SOD activity and the expression of bone-specific genes in differentiated cells compared to control groups in vitro.
Conclusions
In conclusion, the fundings from this study suggest that OAA possesses favorable properties that make it a potential candidate for application in medical bone regeneration.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-024-09389-6</identifier><identifier>PMID: 38536507</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>acridine orange ; Adipose tissue ; alizarin ; Alkaline phosphatase ; Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; bone formation ; Bone growth ; calcium ; catalase ; Cell differentiation ; collagen ; Collagen (type I) ; energy ; Enzymatic activity ; enzyme activity ; Histology ; humans ; Life Sciences ; Mesenchymal stem cells ; metabolism ; Mineralization ; Mitochondria ; Morphology ; Organelles ; Original Article ; Osteocalcin ; oxaloacetic acid ; Regeneration ; reverse transcriptase polymerase chain reaction ; Stem cells ; Structure-function relationships ; Superoxide dismutase ; toxicity testing ; viability</subject><ispartof>Molecular biology reports, 2024-12, Vol.51 (1), p.451-451, Article 451</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024 Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><rights>Copyright Springer Nature B.V. Dec 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-3f849962ae8fa7c3a7809317a380f67f8103aae3d1937f5ca2fea350c27afe003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-024-09389-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-024-09389-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38536507$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shirkoohi, Fatemeh Jamali</creatorcontrib><creatorcontrib>Ghollasi, Marzieh</creatorcontrib><creatorcontrib>Halabian, Raheleh</creatorcontrib><creatorcontrib>Eftekhari, Elahe</creatorcontrib><creatorcontrib>Ghiasi, Mohsen</creatorcontrib><title>Oxaloacetate as new inducer for osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells in vitro</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
Mitochondrial organelles play a crucial role in cellular metabolism so different cell types exhibit diverse metabolic and energy demands. Therefore, alternations in the intracellular distribution, quantity, function, and structure of mitochondria are required for stem cell differentiation. Finding an effective inducer capable of modulating mitochondrial activity is critical for the differentiation of specific stem cells into osteo-like cells for addressing issues related to osteogenic disorders. This study aimed to investigate the effect of oxaloacetate (OAA) on the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADSCs) in vitro.
Methods and results
First, the most favorable OAA concentration was measured through MTT assay and subsequently confirmed using acridine orange staining. Human ADSCs were cultured in osteogenic medium supplemented with OAA and analyzed on days 7 and 14 of differentiation. Various assays including alkaline phosphatase assay (ALP), cellular calcium content assay, mineralized matrix staining with alizarin red, catalase (CAT) and superoxide dismutase (SOD) activity, and real-time RT-PCR analysis of three bone-specific markers (ALP, osteocalcin, and collagen type I) were conducted to characterize the differentiated cells. Following viability assessment, OAA at a concentration of 1 µM was considered the optimal dosage for further studies. The results of osteogenic differentiation assays showed that OAA at a concentration of 1 × 10
− 6
M significantly increased ALP enzyme activity, mineralization, CAT and SOD activity and the expression of bone-specific genes in differentiated cells compared to control groups in vitro.
Conclusions
In conclusion, the fundings from this study suggest that OAA possesses favorable properties that make it a potential candidate for application in medical bone regeneration.</description><subject>acridine orange</subject><subject>Adipose tissue</subject><subject>alizarin</subject><subject>Alkaline phosphatase</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>bone formation</subject><subject>Bone growth</subject><subject>calcium</subject><subject>catalase</subject><subject>Cell differentiation</subject><subject>collagen</subject><subject>Collagen (type I)</subject><subject>energy</subject><subject>Enzymatic activity</subject><subject>enzyme activity</subject><subject>Histology</subject><subject>humans</subject><subject>Life Sciences</subject><subject>Mesenchymal stem cells</subject><subject>metabolism</subject><subject>Mineralization</subject><subject>Mitochondria</subject><subject>Morphology</subject><subject>Organelles</subject><subject>Original Article</subject><subject>Osteocalcin</subject><subject>oxaloacetic acid</subject><subject>Regeneration</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>Stem cells</subject><subject>Structure-function relationships</subject><subject>Superoxide dismutase</subject><subject>toxicity testing</subject><subject>viability</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkU1v1DAQhi1ERbcLf4ADssSll9BxHMfOEVWUIlXqBc7R4IxbV4m92E6h_Hq8bAGJA5x88DPvfDyMvRTwRgDosywESNlA2zUwSDM0_RO2EUrLphu0eco2IEE0nVHimJ3kfAcAndDqGTuWRslegd6w79ffcI5oqWAhjpkH-sp9mFZLibuYeMyF4g0Fb_nknaNEoXgsPgYeHb9dFwwcJ7-LmXjxOa_UTJT8PU18oUzB3j4sOPOasnBL85xrOr_3JcXn7MjhnOnF47tlny7efTy_bK6u3384f3vVWKmG0khnumHoWyTjUFuJ2tRthUZpwPXamXoERJKTGKR2ymLrCKUC22p0BCC37PSQu0vxy0q5jIvP-1EwUFzzKEU9RtsC9P9HQXQAg6k33rLXf6F3cU2hLlKpqkX1rdz3bg-UTTHnRG7cJb9gehgFjHuJ40HiWCWOPyWO-ylePUavnxeafpf8slYBeQBy_Qo3lP70_kfsD5mzqGo</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Shirkoohi, Fatemeh Jamali</creator><creator>Ghollasi, Marzieh</creator><creator>Halabian, Raheleh</creator><creator>Eftekhari, Elahe</creator><creator>Ghiasi, Mohsen</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20241201</creationdate><title>Oxaloacetate as new inducer for osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells in vitro</title><author>Shirkoohi, Fatemeh Jamali ; Ghollasi, Marzieh ; Halabian, Raheleh ; Eftekhari, Elahe ; Ghiasi, Mohsen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-3f849962ae8fa7c3a7809317a380f67f8103aae3d1937f5ca2fea350c27afe003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>acridine orange</topic><topic>Adipose tissue</topic><topic>alizarin</topic><topic>Alkaline phosphatase</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>bone formation</topic><topic>Bone growth</topic><topic>calcium</topic><topic>catalase</topic><topic>Cell differentiation</topic><topic>collagen</topic><topic>Collagen (type I)</topic><topic>energy</topic><topic>Enzymatic activity</topic><topic>enzyme activity</topic><topic>Histology</topic><topic>humans</topic><topic>Life Sciences</topic><topic>Mesenchymal stem cells</topic><topic>metabolism</topic><topic>Mineralization</topic><topic>Mitochondria</topic><topic>Morphology</topic><topic>Organelles</topic><topic>Original Article</topic><topic>Osteocalcin</topic><topic>oxaloacetic acid</topic><topic>Regeneration</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>Stem cells</topic><topic>Structure-function relationships</topic><topic>Superoxide dismutase</topic><topic>toxicity testing</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shirkoohi, Fatemeh Jamali</creatorcontrib><creatorcontrib>Ghollasi, Marzieh</creatorcontrib><creatorcontrib>Halabian, Raheleh</creatorcontrib><creatorcontrib>Eftekhari, Elahe</creatorcontrib><creatorcontrib>Ghiasi, Mohsen</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shirkoohi, Fatemeh Jamali</au><au>Ghollasi, Marzieh</au><au>Halabian, Raheleh</au><au>Eftekhari, Elahe</au><au>Ghiasi, Mohsen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oxaloacetate as new inducer for osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells in vitro</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>51</volume><issue>1</issue><spage>451</spage><epage>451</epage><pages>451-451</pages><artnum>451</artnum><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
Mitochondrial organelles play a crucial role in cellular metabolism so different cell types exhibit diverse metabolic and energy demands. Therefore, alternations in the intracellular distribution, quantity, function, and structure of mitochondria are required for stem cell differentiation. Finding an effective inducer capable of modulating mitochondrial activity is critical for the differentiation of specific stem cells into osteo-like cells for addressing issues related to osteogenic disorders. This study aimed to investigate the effect of oxaloacetate (OAA) on the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADSCs) in vitro.
Methods and results
First, the most favorable OAA concentration was measured through MTT assay and subsequently confirmed using acridine orange staining. Human ADSCs were cultured in osteogenic medium supplemented with OAA and analyzed on days 7 and 14 of differentiation. Various assays including alkaline phosphatase assay (ALP), cellular calcium content assay, mineralized matrix staining with alizarin red, catalase (CAT) and superoxide dismutase (SOD) activity, and real-time RT-PCR analysis of three bone-specific markers (ALP, osteocalcin, and collagen type I) were conducted to characterize the differentiated cells. Following viability assessment, OAA at a concentration of 1 µM was considered the optimal dosage for further studies. The results of osteogenic differentiation assays showed that OAA at a concentration of 1 × 10
− 6
M significantly increased ALP enzyme activity, mineralization, CAT and SOD activity and the expression of bone-specific genes in differentiated cells compared to control groups in vitro.
Conclusions
In conclusion, the fundings from this study suggest that OAA possesses favorable properties that make it a potential candidate for application in medical bone regeneration.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>38536507</pmid><doi>10.1007/s11033-024-09389-6</doi><tpages>1</tpages></addata></record> |
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| subjects | acridine orange Adipose tissue alizarin Alkaline phosphatase Animal Anatomy Animal Biochemistry Biomedical and Life Sciences bone formation Bone growth calcium catalase Cell differentiation collagen Collagen (type I) energy Enzymatic activity enzyme activity Histology humans Life Sciences Mesenchymal stem cells metabolism Mineralization Mitochondria Morphology Organelles Original Article Osteocalcin oxaloacetic acid Regeneration reverse transcriptase polymerase chain reaction Stem cells Structure-function relationships Superoxide dismutase toxicity testing viability |
| title | Oxaloacetate as new inducer for osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells in vitro |
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