Highly efficient expression of Rasamsonia emersonii lipase in Pichia pastoris: characterization and gastrointestinal simulated digestion in vitro

BACKGROUND Acidic lipases with high catalytic activities under acidic conditions have important application values in the food, feed and pharmaceutical industries. However, the availability of acidic lipases is still the main obstacle to their industrial applications. Although a novel acidic lipase...

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Veröffentlicht in:	Journal of the science of food and agriculture 2024-07, Vol.104 (9), p.5603-5613
Hauptverfasser:	Wang, Buqing, Wang, Yasen, Zhou, Xiaoman, Gao, Xiao‐Dong, Fujita, Morihisa, Li, Zijie
Format:	Artikel
Sprache:	eng
Schlagworte:	acidic lipase agriculture biodiesel Biodiesel fuels Biofuels Bioreactors Biosynthesis calcium Calcium ions carboxylic ester hydrolases codon usage Combinatorial analysis culture flasks Digestion Digestive system E coli Enzymatic activity Enzyme activity Escherichia coli Fermentation Food industry gastrointestinal simulated digestion Gastrointestinal tract in vitro digestion Industrial applications Intestine intestines Komagataella pastoris Lipase lipid metabolism Lipids methanol molecular chaperone molecular chaperones Optimization Pepsin Pharmaceutical industry Pichia pastoris Rasamsonia secretion signal peptide sodium stomach Trypsin vacuoles
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